BLASTX nr result
ID: Bupleurum21_contig00015497
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00015497
(1691 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002510725.1| hydroxysteroid dehydrogenase, putative [Rici... 736 0.0
ref|XP_002275214.1| PREDICTED: short-chain dehydrogenase/reducta... 728 0.0
gb|AFX66971.1| 3-beta-hydroxy-delta5-steroid dehydrogenase [Sola... 721 0.0
emb|CBI40764.3| unnamed protein product [Vitis vinifera] 715 0.0
ref|XP_004145560.1| PREDICTED: short-chain dehydrogenase/reducta... 689 0.0
>ref|XP_002510725.1| hydroxysteroid dehydrogenase, putative [Ricinus communis]
gi|223551426|gb|EEF52912.1| hydroxysteroid dehydrogenase,
putative [Ricinus communis]
Length = 478
Score = 736 bits (1899), Expect = 0.0
Identities = 347/477 (72%), Positives = 410/477 (85%)
Frame = -3
Query: 1680 MHLSENEGIERKXXXXXXXXXXXGSALCVELIRRGAFQVRAFDLVSTSSWSHTLLQNGVK 1501
MHL+ENEGIE GSALC+EL+RRGA QVRAFD+ TS WSH L GV
Sbjct: 1 MHLTENEGIENNNFAVTGGLGFVGSALCLELMRRGARQVRAFDVRPTSPWSHQLTSRGVH 60
Query: 1500 CIQGDITSKKDVDRALRGADCVFHLASFGMSGKEMLQYGRIDQVNINGTCHVLDACLDHG 1321
CI+GD+T K DV++ALRG DCVFHLASFGMSGKEM+++GR+D+VNINGTCHVL+AC++ G
Sbjct: 61 CIRGDLTRKSDVEKALRGVDCVFHLASFGMSGKEMIRFGRVDEVNINGTCHVLEACIEFG 120
Query: 1320 IKRLVYVSTYNVVFGGKEIVNGNETLPYFPMDDHADPYGRSKAIAEQLVLKSNGRPFKEK 1141
IKRLVYVSTYNVVFGGK+IVNGNE+LPYFP DDH D YG SK+IAEQLVLK NGRP K+
Sbjct: 121 IKRLVYVSTYNVVFGGKQIVNGNESLPYFPTDDHVDSYGGSKSIAEQLVLKYNGRPLKKN 180
Query: 1140 NGKCLYTCAIRPAAIYGPGEQRHLPRIVNLAKLGLLPFRIGEANVKTDWVYVDNLVLALI 961
NGK LYTCAIRPAAIYGPGE+RH+PRIV+ AKLGL+PFRIG+ANVKTDWVYVDNLVLALI
Sbjct: 181 NGKRLYTCAIRPAAIYGPGEERHMPRIVSYAKLGLMPFRIGDANVKTDWVYVDNLVLALI 240
Query: 960 MASMGLLDDIPGRGSQPVAAGQPYFISDGSPVNTFVFLRPLLQSLEYDMPKASLGVPRAL 781
+ASMGLLDDIP G PVAAGQPYFISDGSP+N+F FL+PLL+SL YDMPKASL VP AL
Sbjct: 241 LASMGLLDDIPNSGGHPVAAGQPYFISDGSPINSFEFLQPLLKSLNYDMPKASLSVPHAL 300
Query: 780 LLGKFFWALYTIMHPLLNKWWLPEPLMLPTEVHKVGVTHHFSLLKARQELGYIPLVSPQE 601
+LGK F A+YT+++P LN+WWLP+P +LP EV+KVGVTH+FS LKA++ELGY+P+V+P+E
Sbjct: 301 ILGKIFGAIYTLLYPWLNRWWLPQPFILPAEVYKVGVTHYFSFLKAKEELGYVPMVTPRE 360
Query: 600 GMAATISYWQDKKRKSLDGPSIFTWILAVGGMLAVFAAAYLPDIGPVPIIRALALFIFRS 421
GMAATI+YWQ+KKRKSLDGP+I+ W+ AV GM +F AAYLPDIGPVP+ RA++LF RS
Sbjct: 361 GMAATIAYWQEKKRKSLDGPTIYIWLFAVIGMSTLFCAAYLPDIGPVPLFRAISLFFLRS 420
Query: 420 LWGIRVLFFLASAAHMGEGIYAYQLAKKVDPANALGWFWQTTALGMFSLRYLLKRAK 250
+ R +F LASAAH+GE IYA+ LAK+VDPANA GWFWQT ALG+FSLR+LLKRA+
Sbjct: 421 MRMTRTVFLLASAAHIGESIYAWHLAKRVDPANARGWFWQTLALGIFSLRFLLKRAR 477
>ref|XP_002275214.1| PREDICTED: short-chain dehydrogenase/reductase family 42E member 1
[Vitis vinifera]
Length = 478
Score = 728 bits (1879), Expect = 0.0
Identities = 344/477 (72%), Positives = 407/477 (85%)
Frame = -3
Query: 1680 MHLSENEGIERKXXXXXXXXXXXGSALCVELIRRGAFQVRAFDLVSTSSWSHTLLQNGVK 1501
MHLSENEGIE G+ALC+EL+RRGA Q+RA DL STS WS L GV
Sbjct: 1 MHLSENEGIEGSTFVVTGGLGFVGAALCLELVRRGARQIRAIDLRSTSPWSDDLENKGVH 60
Query: 1500 CIQGDITSKKDVDRALRGADCVFHLASFGMSGKEMLQYGRIDQVNINGTCHVLDACLDHG 1321
CIQGDIT KKDV+RALRGADCVFHLAS+GMSGKEM+QYGR+D+VNINGTCH+LDAC++ G
Sbjct: 61 CIQGDITVKKDVERALRGADCVFHLASYGMSGKEMIQYGRVDEVNINGTCHILDACIEFG 120
Query: 1320 IKRLVYVSTYNVVFGGKEIVNGNETLPYFPMDDHADPYGRSKAIAEQLVLKSNGRPFKEK 1141
IKRLVY STYNVVFGGKEI+NGNE LPYFP+DDH D Y RSK+IAEQLVLK+NGRPFK K
Sbjct: 121 IKRLVYTSTYNVVFGGKEILNGNEALPYFPLDDHVDSYSRSKSIAEQLVLKNNGRPFKNK 180
Query: 1140 NGKCLYTCAIRPAAIYGPGEQRHLPRIVNLAKLGLLPFRIGEANVKTDWVYVDNLVLALI 961
+GKCLYTCA+RPAAIYGPGE RH PRI++LAKLG+LPF IGEANVK DW+YVDNLV A I
Sbjct: 181 SGKCLYTCAVRPAAIYGPGEDRHFPRIISLAKLGVLPFTIGEANVKGDWIYVDNLVHAQI 240
Query: 960 MASMGLLDDIPGRGSQPVAAGQPYFISDGSPVNTFVFLRPLLQSLEYDMPKASLGVPRAL 781
+ASMGLLDDIPGR +P+AAGQ YFI+DGSPVN + FLRPLL+SLEYD+PKASL VP AL
Sbjct: 241 LASMGLLDDIPGREKRPIAAGQSYFINDGSPVNIYEFLRPLLRSLEYDLPKASLPVPYAL 300
Query: 780 LLGKFFWALYTIMHPLLNKWWLPEPLMLPTEVHKVGVTHHFSLLKARQELGYIPLVSPQE 601
+ + +YT+++P LN+WWLP+PLMLP EV+KVGVTH+FS LKA++ELGY+PLVSP+E
Sbjct: 301 FMSRINCMIYTLLYPWLNRWWLPQPLMLPAEVYKVGVTHYFSYLKAKEELGYVPLVSPRE 360
Query: 600 GMAATISYWQDKKRKSLDGPSIFTWILAVGGMLAVFAAAYLPDIGPVPIIRALALFIFRS 421
GMAATISYWQ++KR+SL+GP++ TW+ + GM +F AAYLPDIGPVPI RA++LF RS
Sbjct: 361 GMAATISYWQERKRRSLEGPTLQTWLFCIIGMFVLFCAAYLPDIGPVPIFRAISLFFLRS 420
Query: 420 LWGIRVLFFLASAAHMGEGIYAYQLAKKVDPANALGWFWQTTALGMFSLRYLLKRAK 250
+ IRV+F LA+AAH+GE +YA+ LAK+VDPANA GWFWQT ALG+FSLR+LLKRAK
Sbjct: 421 MAIIRVVFLLATAAHIGEAVYAWHLAKRVDPANARGWFWQTFALGIFSLRFLLKRAK 477
>gb|AFX66971.1| 3-beta-hydroxy-delta5-steroid dehydrogenase [Solanum tuberosum]
Length = 478
Score = 721 bits (1862), Expect = 0.0
Identities = 342/477 (71%), Positives = 405/477 (84%)
Frame = -3
Query: 1680 MHLSENEGIERKXXXXXXXXXXXGSALCVELIRRGAFQVRAFDLVSTSSWSHTLLQNGVK 1501
MHLSENEGIE+K G+ALC+EL+RRGA V+AFDL + S WS L Q GV
Sbjct: 1 MHLSENEGIEKKSFVVTGGLGFIGAALCLELVRRGARLVKAFDLRTHSPWSSQLRQYGVH 60
Query: 1500 CIQGDITSKKDVDRALRGADCVFHLASFGMSGKEMLQYGRIDQVNINGTCHVLDACLDHG 1321
IQGDIT K+ V +AL+G+DCVFHLAS+GMSGKEMLQY R+D+VNINGTCH++DACLDH
Sbjct: 61 LIQGDITDKQHVQKALQGSDCVFHLASYGMSGKEMLQYSRVDEVNINGTCHIIDACLDHQ 120
Query: 1320 IKRLVYVSTYNVVFGGKEIVNGNETLPYFPMDDHADPYGRSKAIAEQLVLKSNGRPFKEK 1141
IKRLVYVST NVV+GGKEIVNGNE LPYFP+DDH DPYGRSK+IAEQLVLKSNGRPF +K
Sbjct: 121 IKRLVYVSTPNVVYGGKEIVNGNENLPYFPIDDHVDPYGRSKSIAEQLVLKSNGRPFTKK 180
Query: 1140 NGKCLYTCAIRPAAIYGPGEQRHLPRIVNLAKLGLLPFRIGEANVKTDWVYVDNLVLALI 961
NGKCLYTCAIRPAAIYGPGE+RHLPRI+ L KLGL PF+IG NVK+DWVYVDNLVLAL+
Sbjct: 181 NGKCLYTCAIRPAAIYGPGEERHLPRIITLTKLGLFPFKIGSPNVKSDWVYVDNLVLALL 240
Query: 960 MASMGLLDDIPGRGSQPVAAGQPYFISDGSPVNTFVFLRPLLQSLEYDMPKASLGVPRAL 781
+ASMGLLDDIPGR P+AAGQPYFISDGSP+N+F FL PLL+SL+YD+PK SL V AL
Sbjct: 241 LASMGLLDDIPGREGLPIAAGQPYFISDGSPINSFEFLLPLLKSLDYDLPKTSLAVSHAL 300
Query: 780 LLGKFFWALYTIMHPLLNKWWLPEPLMLPTEVHKVGVTHHFSLLKARQELGYIPLVSPQE 601
LLGK FWA Y+ ++P LN WLP+PL+LP EV+KVGVTH+FS LKA++ELGY+P+VS +E
Sbjct: 301 LLGKIFWAFYSFLYPWLNSRWLPQPLILPAEVYKVGVTHYFSFLKAKEELGYVPMVSSKE 360
Query: 600 GMAATISYWQDKKRKSLDGPSIFTWILAVGGMLAVFAAAYLPDIGPVPIIRALALFIFRS 421
GMAATI+YWQ++KR+SLDGP+I+ W+ V GM A+FAAAYLPD GP+P IRA+ LF FRS
Sbjct: 361 GMAATIAYWQERKRRSLDGPTIWAWLFCVIGMSALFAAAYLPDYGPIPFIRAVHLFFFRS 420
Query: 420 LWGIRVLFFLASAAHMGEGIYAYQLAKKVDPANALGWFWQTTALGMFSLRYLLKRAK 250
+ ++V+F LA+AAH+GE IYA+ +AK +DPANA GWFWQT ALG+FSLR LLKRAK
Sbjct: 421 MLALKVIFVLAAAAHVGEAIYAWNVAKTIDPANARGWFWQTFALGIFSLRLLLKRAK 477
>emb|CBI40764.3| unnamed protein product [Vitis vinifera]
Length = 479
Score = 715 bits (1845), Expect = 0.0
Identities = 337/470 (71%), Positives = 400/470 (85%)
Frame = -3
Query: 1680 MHLSENEGIERKXXXXXXXXXXXGSALCVELIRRGAFQVRAFDLVSTSSWSHTLLQNGVK 1501
MHLSENEGIE G+ALC+EL+RRGA Q+RA DL STS WS L GV
Sbjct: 1 MHLSENEGIEGSTFVVTGGLGFVGAALCLELVRRGARQIRAIDLRSTSPWSDDLENKGVH 60
Query: 1500 CIQGDITSKKDVDRALRGADCVFHLASFGMSGKEMLQYGRIDQVNINGTCHVLDACLDHG 1321
CIQGDIT KKDV+RALRGADCVFHLAS+GMSGKEM+QYGR+D+VNINGTCH+LDAC++ G
Sbjct: 61 CIQGDITVKKDVERALRGADCVFHLASYGMSGKEMIQYGRVDEVNINGTCHILDACIEFG 120
Query: 1320 IKRLVYVSTYNVVFGGKEIVNGNETLPYFPMDDHADPYGRSKAIAEQLVLKSNGRPFKEK 1141
IKRLVY STYNVVFGGKEI+NGNE LPYFP+DDH D Y RSK+IAEQLVLK+NGRPFK K
Sbjct: 121 IKRLVYTSTYNVVFGGKEILNGNEALPYFPLDDHVDSYSRSKSIAEQLVLKNNGRPFKNK 180
Query: 1140 NGKCLYTCAIRPAAIYGPGEQRHLPRIVNLAKLGLLPFRIGEANVKTDWVYVDNLVLALI 961
+GKCLYTCA+RPAAIYGPGE RH PRI++LAKLG+LPF IGEANVK DW+YVDNLV A I
Sbjct: 181 SGKCLYTCAVRPAAIYGPGEDRHFPRIISLAKLGVLPFTIGEANVKGDWIYVDNLVHAQI 240
Query: 960 MASMGLLDDIPGRGSQPVAAGQPYFISDGSPVNTFVFLRPLLQSLEYDMPKASLGVPRAL 781
+ASMGLLDDIPGR +P+AAGQ YFI+DGSPVN + FLRPLL+SLEYD+PKASL VP AL
Sbjct: 241 LASMGLLDDIPGREKRPIAAGQSYFINDGSPVNIYEFLRPLLRSLEYDLPKASLPVPYAL 300
Query: 780 LLGKFFWALYTIMHPLLNKWWLPEPLMLPTEVHKVGVTHHFSLLKARQELGYIPLVSPQE 601
+ + +YT+++P LN+WWLP+PLMLP EV+KVGVTH+FS LKA++ELGY+PLVSP+E
Sbjct: 301 FMSRINCMIYTLLYPWLNRWWLPQPLMLPAEVYKVGVTHYFSYLKAKEELGYVPLVSPRE 360
Query: 600 GMAATISYWQDKKRKSLDGPSIFTWILAVGGMLAVFAAAYLPDIGPVPIIRALALFIFRS 421
GMAATISYWQ++KR+SL+GP++ TW+ + GM +F AAYLPDIGPVPI RA++LF RS
Sbjct: 361 GMAATISYWQERKRRSLEGPTLQTWLFCIIGMFVLFCAAYLPDIGPVPIFRAISLFFLRS 420
Query: 420 LWGIRVLFFLASAAHMGEGIYAYQLAKKVDPANALGWFWQTTALGMFSLR 271
+ IRV+F LA+AAH+GE +YA+ LAK+VDPANA GWFWQT ALG+FSL+
Sbjct: 421 MAIIRVVFLLATAAHIGEAVYAWHLAKRVDPANARGWFWQTFALGIFSLQ 470
>ref|XP_004145560.1| PREDICTED: short-chain dehydrogenase/reductase family 42E member
1-like [Cucumis sativus] gi|449522962|ref|XP_004168494.1|
PREDICTED: short-chain dehydrogenase/reductase family 42E
member 1-like [Cucumis sativus]
Length = 478
Score = 689 bits (1778), Expect = 0.0
Identities = 323/478 (67%), Positives = 397/478 (83%)
Frame = -3
Query: 1680 MHLSENEGIERKXXXXXXXXXXXGSALCVELIRRGAFQVRAFDLVSTSSWSHTLLQNGVK 1501
MHLS +EGIE GSALC+EL+RRGA QVRAFDL S WS L GVK
Sbjct: 1 MHLSASEGIEGNTFTVTGGLGFAGSALCLELLRRGALQVRAFDLRPASPWSDHLKTQGVK 60
Query: 1500 CIQGDITSKKDVDRALRGADCVFHLASFGMSGKEMLQYGRIDQVNINGTCHVLDACLDHG 1321
IQGD+T KKDV+RALRG DCVFHLA++G+SGKEMLQ GRID+VNINGTCHVLDACL+ G
Sbjct: 61 IIQGDVTCKKDVERALRGVDCVFHLAAYGLSGKEMLQVGRIDEVNINGTCHVLDACLEFG 120
Query: 1320 IKRLVYVSTYNVVFGGKEIVNGNETLPYFPMDDHADPYGRSKAIAEQLVLKSNGRPFKEK 1141
++RL+Y+STYNVVFG +EIVNGNE LPY P+DDH D YGRSK+IAEQLVLK+NGRP K +
Sbjct: 121 VRRLIYMSTYNVVFGSQEIVNGNEGLPYLPIDDHVDAYGRSKSIAEQLVLKTNGRPLKNR 180
Query: 1140 NGKCLYTCAIRPAAIYGPGEQRHLPRIVNLAKLGLLPFRIGEANVKTDWVYVDNLVLALI 961
NGKCL+TCAIR AIYGPGE+RH R+V+LAKLGLLPFR+G+ + KTDW+YVDNLVLALI
Sbjct: 181 NGKCLHTCAIRSCAIYGPGEERHFTRLVSLAKLGLLPFRVGKQSAKTDWIYVDNLVLALI 240
Query: 960 MASMGLLDDIPGRGSQPVAAGQPYFISDGSPVNTFVFLRPLLQSLEYDMPKASLGVPRAL 781
+ASMGLLDDIPG+G PVAAGQPY++SDG PVN+F F++PLL SL YD+P L VP+AL
Sbjct: 241 LASMGLLDDIPGKGKDPVAAGQPYYVSDGHPVNSFEFVKPLLNSLGYDLPNYYLPVPKAL 300
Query: 780 LLGKFFWALYTIMHPLLNKWWLPEPLMLPTEVHKVGVTHHFSLLKARQELGYIPLVSPQE 601
LGKFF LYTI++P L++WWLP PLMLP E++KVGV+++FS LKA++ELGY P+V+P+E
Sbjct: 301 PLGKFFALLYTILYPWLDRWWLPHPLMLPAEIYKVGVSNYFSYLKAKEELGYAPIVTPKE 360
Query: 600 GMAATISYWQDKKRKSLDGPSIFTWILAVGGMLAVFAAAYLPDIGPVPIIRALALFIFRS 421
GMAATISYWQ+++RKSLDGP+I+ W+ + GM +F AA+LPD+GPVP +A++LF FRS
Sbjct: 361 GMAATISYWQERERKSLDGPTIYVWLFCLVGMSILFCAAFLPDVGPVPFFKAISLFFFRS 420
Query: 420 LWGIRVLFFLASAAHMGEGIYAYQLAKKVDPANALGWFWQTTALGMFSLRYLLKRAKN 247
+ +R++F +A H+GE IYA+ LA+KVDPAN+ GWFWQT ALG FSLR+LLKRA+N
Sbjct: 421 IKVLRMVFLVALLLHVGEAIYAWFLARKVDPANSRGWFWQTFALGFFSLRFLLKRARN 478