BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00015352
         (1302 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002325942.1| tubulin folding cofactor [Populus trichocarp...   318   e-126
ref|XP_002275735.1| PREDICTED: tubulin-specific chaperone E [Vit...   323   e-124
ref|XP_002525519.1| tubulin-specific chaperone E, putative [Rici...   317   e-122
ref|NP_565017.1| tubulin folding cofactor E / Pfifferling (PFI) ...   325   e-121
gb|AAM22962.1|AF486853_1 tubulin folding cofactor E [Arabidopsis...   324   e-121

>ref|XP_002325942.1| tubulin folding cofactor [Populus trichocarpa]
            gi|222862817|gb|EEF00324.1| tubulin folding cofactor
            [Populus trichocarpa]
          Length = 537

 Score =  318 bits (815), Expect(2) = e-126
 Identities = 158/234 (67%), Positives = 193/234 (82%), Gaps = 1/234 (0%)
 Frame = -3

Query: 1183 KIGQRVHSVGDCRRIGTVKYTGSVEGYSGNWVGVEWDNEGDGKHDGSVNGVRYFQANSPT 1004
            K+ QRVHS  D RRIGTVKY G VEG+ G WVGV+WDN G+ KHDGS+NGVRYF+A S  
Sbjct: 11   KLDQRVHSTNDPRRIGTVKYIGPVEGHPGTWVGVDWDN-GEAKHDGSLNGVRYFEARSQL 69

Query: 1003 SASFVRPHNLSSGISLLQALDLRYRTASTKEEEDEMYVFSANNRRVSVQLLGKDKIQDKL 824
            S SFVR  NL++GIS ++AL +RYR   T+E+EDEMYV SA+N+RVSVQL+GK+KIQDKL
Sbjct: 70   SGSFVRAQNLTAGISFIEALYIRYRDQPTQEDEDEMYVLSASNKRVSVQLVGKEKIQDKL 129

Query: 823  NQFQDLRSASLFYLGVSSCGHPSQISASVPNLKELDLTGNLLSKWEDIGNICDALPSLVA 644
            ++ ++L  ASL YLGVS+ G P++I   VPNLKELDLTGNLLS+W+D+G IC+ LPSL A
Sbjct: 130  SRLEELTGASLSYLGVSNPGSPNEIRNIVPNLKELDLTGNLLSEWKDVGIICEQLPSLAA 189

Query: 643  LNLSYNKMSHDLVGLQ-LKSIKILVLNNTGLNWTQVAILENSLPVIEELHLMGN 485
            LNLS N MSH++VGL  LKSI ILVLNNTG+NWTQ+ +L++ LPVIEELHLMGN
Sbjct: 190  LNLSNNSMSHEIVGLPLLKSIHILVLNNTGINWTQIEVLKDLLPVIEELHLMGN 243



 Score =  160 bits (405), Expect(2) = e-126
 Identities = 82/137 (59%), Positives = 99/137 (72%)
 Frame = -1

Query: 411 VSDIQPMSSTAVKGFNSLRLLNLEDNCLADWSEIXXXXXXXXXXXXXLNKNKINCIRYPG 232
           ++ I+  SS+ V GF+SLRLLNLE+NC+A+W+EI             LNKN +N I YP 
Sbjct: 245 INAIKTASSSIVHGFDSLRLLNLEENCIAEWNEIVKLSQLRSLEELHLNKNNLNHIFYPD 304

Query: 231 KDEIQKLVNHYGSSENSFVPFPNLSCLLIGGNNIGDLASLDSLNSFPRLTDIRLSENPIT 52
            D I KLV    S + S +PF NL CLL+GGNNI DLAS+DSLNSFP+L DIRLSENPI 
Sbjct: 305 HDTIDKLVGGDESHDQSCIPFQNLRCLLLGGNNIDDLASVDSLNSFPKLIDIRLSENPIA 364

Query: 51  DPGKGGIARFVLIARLA 1
           DPG+GGI RFVL+ARLA
Sbjct: 365 DPGRGGIPRFVLVARLA 381


>ref|XP_002275735.1| PREDICTED: tubulin-specific chaperone E [Vitis vinifera]
            gi|297741006|emb|CBI31318.3| unnamed protein product
            [Vitis vinifera]
          Length = 541

 Score =  323 bits (827), Expect(2) = e-124
 Identities = 157/234 (67%), Positives = 198/234 (84%), Gaps = 1/234 (0%)
 Frame = -3

Query: 1183 KIGQRVHSVGDCRRIGTVKYTGSVEGYSGNWVGVEWDNEGDGKHDGSVNGVRYFQANSPT 1004
            ++GQRVHS+GD RR+GTVKY G V+GYSG WVGV+WDN GD KHDG+++G RYFQA++  
Sbjct: 14   RLGQRVHSLGDPRRMGTVKYVGPVQGYSGTWVGVDWDN-GDAKHDGALDGRRYFQAHAAK 72

Query: 1003 SASFVRPHNLSSGISLLQALDLRYRTASTKEEEDEMYVFSANNRRVSVQLLGKDKIQDKL 824
            S SFVRPHNLS+GISLLQAL LRYR+ ++KEEE+EMYV SA+NRRVSVQL+GK++I+DKL
Sbjct: 73   SGSFVRPHNLSAGISLLQALLLRYRSTTSKEEEEEMYVLSASNRRVSVQLVGKEQIEDKL 132

Query: 823  NQFQDLRSASLFYLGVSSCGHPSQISASVPNLKELDLTGNLLSKWEDIGNICDALPSLVA 644
            ++F++L +ASL YLGVSS G P +I + VPNLKELDLTGNLLS+W+D+G IC  LP L A
Sbjct: 133  SRFEELTAASLSYLGVSSIGAPFEICSVVPNLKELDLTGNLLSEWKDVGTICVQLPGLAA 192

Query: 643  LNLSYNKMSHDLVGLQ-LKSIKILVLNNTGLNWTQVAILENSLPVIEELHLMGN 485
            LNLS N M+HD+ GL  L ++++LVLNNTG+ W +V I+ +SLP IEELHLMGN
Sbjct: 193  LNLSNNLMAHDITGLPLLMNLRVLVLNNTGIKWKEVEIIRHSLPAIEELHLMGN 246



 Score =  150 bits (378), Expect(2) = e-124
 Identities = 79/134 (58%), Positives = 92/134 (68%)
 Frame = -1

Query: 402 IQPMSSTAVKGFNSLRLLNLEDNCLADWSEIXXXXXXXXXXXXXLNKNKINCIRYPGKDE 223
           I P SS+ V+GF+ LRLLNLEDN +A+W EI             LNKN +  I YP  D 
Sbjct: 251 ITPASSSIVQGFDYLRLLNLEDNHIAEWDEILKLSQLRSLEQLHLNKNHLKHIFYPDSDA 310

Query: 222 IQKLVNHYGSSENSFVPFPNLSCLLIGGNNIGDLASLDSLNSFPRLTDIRLSENPITDPG 43
           I +L+N   S E    PF NL CLL+GGNNI DLAS+DSLNSFP L DIRLSENP+ DPG
Sbjct: 311 IHQLLNGIDSLEKGCKPFQNLHCLLLGGNNIEDLASVDSLNSFPMLKDIRLSENPVADPG 370

Query: 42  KGGIARFVLIARLA 1
           +GGI RFVLIARL+
Sbjct: 371 RGGIPRFVLIARLS 384


>ref|XP_002525519.1| tubulin-specific chaperone E, putative [Ricinus communis]
            gi|223535198|gb|EEF36877.1| tubulin-specific chaperone E,
            putative [Ricinus communis]
          Length = 533

 Score =  317 bits (811), Expect(2) = e-122
 Identities = 159/234 (67%), Positives = 191/234 (81%), Gaps = 1/234 (0%)
 Frame = -3

Query: 1183 KIGQRVHSVGDCRRIGTVKYTGSVEGYSGNWVGVEWDNEGDGKHDGSVNGVRYFQANSPT 1004
            K+GQRVHS  D RRIG VKY G VEGYSG WVGV+WDN G+GKHDGSVNGVRYFQA S  
Sbjct: 8    KLGQRVHSASDPRRIGMVKYIGPVEGYSGTWVGVDWDN-GEGKHDGSVNGVRYFQARSDK 66

Query: 1003 SASFVRPHNLSSGISLLQALDLRYRTASTKEEEDEMYVFSANNRRVSVQLLGKDKIQDKL 824
            S SF+R  NL+ GISL++AL +RY+  STKEEEDEMYV SA+N++VSV+ +GK+KI+DKL
Sbjct: 67   SGSFIRVPNLNPGISLVEALHIRYKGESTKEEEDEMYVLSASNKKVSVEFVGKEKIKDKL 126

Query: 823  NQFQDLRSASLFYLGVSSCGHPSQISASVPNLKELDLTGNLLSKWEDIGNICDALPSLVA 644
            ++F +L+SASL +LG+SS G P+ I   VPNLKELDLTGNLLS+W+DIG IC+ LP+L A
Sbjct: 127  SKFDELKSASLPFLGISSPGSPTDIRNLVPNLKELDLTGNLLSEWKDIGTICEQLPALAA 186

Query: 643  LNLSYNKMSHDLVGL-QLKSIKILVLNNTGLNWTQVAILENSLPVIEELHLMGN 485
            LNLS N MS  +  L QLK+I ILVLNNTG+NWTQV +L + LP IEELHLMGN
Sbjct: 187  LNLSNNLMSRTIAELSQLKNIHILVLNNTGINWTQVEVLNHLLPAIEELHLMGN 240



 Score =  149 bits (376), Expect(2) = e-122
 Identities = 79/137 (57%), Positives = 95/137 (69%)
 Frame = -1

Query: 411 VSDIQPMSSTAVKGFNSLRLLNLEDNCLADWSEIXXXXXXXXXXXXXLNKNKINCIRYPG 232
           +  I   SS+ V+GF+SLRLLNLEDN +A+W+EI             LNKN++  I YP 
Sbjct: 242 IGTIMSSSSSIVQGFDSLRLLNLEDNFIAEWNEILKLSSLRSLEQLYLNKNRLKRIFYPD 301

Query: 231 KDEIQKLVNHYGSSENSFVPFPNLSCLLIGGNNIGDLASLDSLNSFPRLTDIRLSENPIT 52
            D I KL+   GS  N  +PF NL CLL+GGN I DLAS+DSLNSFPRL D+RLSENPI 
Sbjct: 302 ADTIDKLLT--GSESNVEIPFQNLRCLLLGGNRIEDLASVDSLNSFPRLVDVRLSENPIA 359

Query: 51  DPGKGGIARFVLIARLA 1
           D G+GGI RFVLIARL+
Sbjct: 360 DTGRGGIPRFVLIARLS 376


>ref|NP_565017.1| tubulin folding cofactor E / Pfifferling (PFI) [Arabidopsis thaliana]
            gi|26452683|dbj|BAC43424.1| unknown protein [Arabidopsis
            thaliana] gi|27311595|gb|AAO00763.1| Unknown protein
            [Arabidopsis thaliana] gi|31711942|gb|AAP68327.1|
            At1g71440 [Arabidopsis thaliana]
            gi|332197080|gb|AEE35201.1| tubulin folding cofactor E /
            Pfifferling (PFI) [Arabidopsis thaliana]
          Length = 531

 Score =  325 bits (833), Expect(2) = e-121
 Identities = 161/233 (69%), Positives = 191/233 (81%), Gaps = 1/233 (0%)
 Frame = -3

Query: 1180 IGQRVHSVGDCRRIGTVKYTGSVEGYSGNWVGVEWDNEGDGKHDGSVNGVRYFQANSPTS 1001
            IGQRVHS+ D RR+GTVKY G VEGYSG W+GV+WD +GDGKH+GSVNGV YF   S +S
Sbjct: 12   IGQRVHSLNDSRRVGTVKYVGDVEGYSGTWIGVDWDQDGDGKHNGSVNGVFYFNGRSQSS 71

Query: 1000 ASFVRPHNLSSGISLLQALDLRYRTASTKEEEDEMYVFSANNRRVSVQLLGKDKIQDKLN 821
            ASFVR  NLS GI+LLQAL+LRYRT STK+EEDEMYV SA NRRVS+QLLG DKIQDKL+
Sbjct: 72   ASFVRSQNLSRGITLLQALELRYRTISTKDEEDEMYVLSAGNRRVSIQLLGGDKIQDKLS 131

Query: 820  QFQDLRSASLFYLGVSSCGHPSQISASVPNLKELDLTGNLLSKWEDIGNICDALPSLVAL 641
            +F++L SASL YLGVSS G  S + + +PNLK LDLTGNL+S WE+IG +C+ LP+L  L
Sbjct: 132  RFEELTSASLSYLGVSSLGVSSDLGSILPNLKLLDLTGNLISDWEEIGALCEQLPALTTL 191

Query: 640  NLSYNKMSHDLVGL-QLKSIKILVLNNTGLNWTQVAILENSLPVIEELHLMGN 485
            NLS N +S D+  L QLK+I++LVLNN+GL+WTQV IL  SLP IEELHLMGN
Sbjct: 192  NLSCNSLSSDIKSLPQLKNIRVLVLNNSGLSWTQVEILRRSLPGIEELHLMGN 244



 Score =  139 bits (351), Expect(2) = e-121
 Identities = 74/136 (54%), Positives = 93/136 (68%)
 Frame = -1

Query: 411 VSDIQPMSSTAVKGFNSLRLLNLEDNCLADWSEIXXXXXXXXXXXXXLNKNKINCIRYPG 232
           +S I   SS+  + FNSLRLLNL+DNC++DWSE+             LNKNK++      
Sbjct: 246 ISTITSTSSSDDQAFNSLRLLNLDDNCISDWSEVLKLSQLPCLEQLYLNKNKLS------ 299

Query: 231 KDEIQKLVNHYGSSENSFVPFPNLSCLLIGGNNIGDLASLDSLNSFPRLTDIRLSENPIT 52
              I + VN   SSE    PFP+LSCLL+G NNIGDLAS+D+LN FP+L DIRLSENPI+
Sbjct: 300 --RIFQSVNGTESSEKGSDPFPSLSCLLLGANNIGDLASVDALNGFPQLVDIRLSENPIS 357

Query: 51  DPGKGGIARFVLIARL 4
           DP +GG+ RFVL+ARL
Sbjct: 358 DPVRGGVPRFVLVARL 373


>gb|AAM22962.1|AF486853_1 tubulin folding cofactor E [Arabidopsis thaliana]
            gi|21554061|gb|AAM63142.1| unknown [Arabidopsis thaliana]
          Length = 531

 Score =  324 bits (830), Expect(2) = e-121
 Identities = 160/233 (68%), Positives = 191/233 (81%), Gaps = 1/233 (0%)
 Frame = -3

Query: 1180 IGQRVHSVGDCRRIGTVKYTGSVEGYSGNWVGVEWDNEGDGKHDGSVNGVRYFQANSPTS 1001
            IGQRVHS+ D RR+GTVKY G VEGYSG W+GV+WD +GDGKH+GSVNGV YF   S +S
Sbjct: 12   IGQRVHSLNDSRRVGTVKYVGDVEGYSGTWIGVDWDQDGDGKHNGSVNGVFYFNGRSQSS 71

Query: 1000 ASFVRPHNLSSGISLLQALDLRYRTASTKEEEDEMYVFSANNRRVSVQLLGKDKIQDKLN 821
            ASFVR  NLS GI+LLQAL+LRYRT STK+EEDEMYV SA NRRVS+QLLG DKIQDKL+
Sbjct: 72   ASFVRSQNLSRGITLLQALELRYRTISTKDEEDEMYVLSAGNRRVSIQLLGGDKIQDKLS 131

Query: 820  QFQDLRSASLFYLGVSSCGHPSQISASVPNLKELDLTGNLLSKWEDIGNICDALPSLVAL 641
            +F++L SASL YLGVSS G  S + + +PNLK LDLTGNL+S WE+IG +C+ LP+L  L
Sbjct: 132  RFEELTSASLSYLGVSSLGVSSDLGSILPNLKLLDLTGNLISDWEEIGALCEQLPALTTL 191

Query: 640  NLSYNKMSHDLVGL-QLKSIKILVLNNTGLNWTQVAILENSLPVIEELHLMGN 485
            NLS N +S D+  L +LK+I++LVLNN+GL+WTQV IL  SLP IEELHLMGN
Sbjct: 192  NLSCNSLSSDIKSLPELKNIRVLVLNNSGLSWTQVEILRRSLPGIEELHLMGN 244



 Score =  139 bits (350), Expect(2) = e-121
 Identities = 74/136 (54%), Positives = 93/136 (68%)
 Frame = -1

Query: 411 VSDIQPMSSTAVKGFNSLRLLNLEDNCLADWSEIXXXXXXXXXXXXXLNKNKINCIRYPG 232
           +S I   SS+  + FNSLRLLNL+DNC++DWSE+             LNKNK++      
Sbjct: 246 ISTITSTSSSDDQAFNSLRLLNLDDNCISDWSEVLKLSQLPCLEQLYLNKNKLS------ 299

Query: 231 KDEIQKLVNHYGSSENSFVPFPNLSCLLIGGNNIGDLASLDSLNSFPRLTDIRLSENPIT 52
              I + VN   SSE    PFP+LSCLL+G NNIGDLAS+D+LN FP+L DIRLSENPI+
Sbjct: 300 --RIFQSVNGTESSEKGSDPFPSLSCLLLGANNIGDLASVDALNGFPQLLDIRLSENPIS 357

Query: 51  DPGKGGIARFVLIARL 4
           DP +GG+ RFVL+ARL
Sbjct: 358 DPVRGGVPRFVLVARL 373