BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00015326
         (268 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002524674.1| Sphingosine kinase, putative [Ricinus commun...   118   4e-25
ref|XP_003545841.1| PREDICTED: sphingosine kinase A-like [Glycin...   117   7e-25
ref|XP_002869884.1| D-erythro-sphingosine kinase [Arabidopsis ly...   115   5e-24
ref|NP_001031688.1| sphingosine kinase 1 [Arabidopsis thaliana] ...   114   8e-24
emb|CAA18718.1| putative protein [Arabidopsis thaliana] gi|72689...   114   8e-24

>ref|XP_002524674.1| Sphingosine kinase, putative [Ricinus communis]
           gi|223536035|gb|EEF37693.1| Sphingosine kinase, putative
           [Ricinus communis]
          Length = 479

 Score =  118 bits (296), Expect = 4e-25
 Identities = 55/81 (67%), Positives = 69/81 (85%)
 Frame = -3

Query: 260 FLPHVVRPKKLLILVNPFGGKKSASKIFTADVKPLLDDANIKYTMQETTHQLHAKEIASK 81
           +L  + RPK+LL+ VNPFGGK+SASKIF   VKPLL+DA+++ T+QET HQLHAKE+ S 
Sbjct: 102 YLDSLGRPKRLLVFVNPFGGKRSASKIFFDTVKPLLEDADVQITLQETKHQLHAKEVTST 161

Query: 80  MDLSMYDGIVCVSGDGILVEM 18
           +D+S YDGIVCVSGDGILVE+
Sbjct: 162 LDISKYDGIVCVSGDGILVEV 182


>ref|XP_003545841.1| PREDICTED: sphingosine kinase A-like [Glycine max]
          Length = 488

 Score =  117 bits (294), Expect = 7e-25
 Identities = 54/81 (66%), Positives = 68/81 (83%)
 Frame = -3

Query: 260 FLPHVVRPKKLLILVNPFGGKKSASKIFTADVKPLLDDANIKYTMQETTHQLHAKEIASK 81
           F+  + RPK+LL+ VNPFGGKKSA+KIF   VKPL +DA+I+ T+QET HQLHAKE+A  
Sbjct: 102 FIDSLGRPKRLLVFVNPFGGKKSATKIFAEQVKPLFEDAHIQITVQETKHQLHAKEVARS 161

Query: 80  MDLSMYDGIVCVSGDGILVEM 18
           +D++ YDGIVCVSGDGILVE+
Sbjct: 162 LDITKYDGIVCVSGDGILVEV 182


>ref|XP_002869884.1| D-erythro-sphingosine kinase [Arabidopsis lyrata subsp. lyrata]
            gi|297315720|gb|EFH46143.1| D-erythro-sphingosine kinase
            [Arabidopsis lyrata subsp. lyrata]
          Length = 1273

 Score =  115 bits (287), Expect = 5e-24
 Identities = 55/75 (73%), Positives = 63/75 (84%)
 Frame = -3

Query: 242  RPKKLLILVNPFGGKKSASKIFTADVKPLLDDANIKYTMQETTHQLHAKEIASKMDLSMY 63
            RPKKLL+ VNPFGGKKSA KIF  +VKPL +DANI+  +QET +QLHAKEI   MD+S Y
Sbjct: 905  RPKKLLVFVNPFGGKKSARKIFQEEVKPLFEDANIQLEIQETKYQLHAKEIVRSMDVSKY 964

Query: 62   DGIVCVSGDGILVEM 18
            DGIVCVSGDGILVE+
Sbjct: 965  DGIVCVSGDGILVEV 979



 Score =  112 bits (279), Expect = 4e-23
 Identities = 52/81 (64%), Positives = 66/81 (81%)
 Frame = -3

Query: 260 FLPHVVRPKKLLILVNPFGGKKSASKIFTADVKPLLDDANIKYTMQETTHQLHAKEIASK 81
           +L  + RPKKLL+LVNPFGGKK A +IF  +VKPL +DA+++  +QET +QLHAKEI   
Sbjct: 380 YLDSLGRPKKLLVLVNPFGGKKLAREIFVKEVKPLFEDADVQLEIQETKYQLHAKEIVKS 439

Query: 80  MDLSMYDGIVCVSGDGILVEM 18
           MD+S YDGIVCVSGDG+LVE+
Sbjct: 440 MDVSKYDGIVCVSGDGVLVEV 460


>ref|NP_001031688.1| sphingosine kinase 1 [Arabidopsis thaliana]
           gi|332659066|gb|AEE84466.1| sphingosine kinase 1
           [Arabidopsis thaliana]
          Length = 320

 Score =  114 bits (285), Expect = 8e-24
 Identities = 57/84 (67%), Positives = 67/84 (79%), Gaps = 3/84 (3%)
 Frame = -3

Query: 260 FLPHVV---RPKKLLILVNPFGGKKSASKIFTADVKPLLDDANIKYTMQETTHQLHAKEI 90
           F  H+V   RPKKLL+ VNPFGGKK+A KIF  +VKPL +DANI+  +QET +QLHAKEI
Sbjct: 108 FHQHLVSLGRPKKLLVFVNPFGGKKTARKIFQEEVKPLFEDANIQLEIQETKYQLHAKEI 167

Query: 89  ASKMDLSMYDGIVCVSGDGILVEM 18
              MD+S YDGIVCVSGDGILVE+
Sbjct: 168 VRSMDVSKYDGIVCVSGDGILVEV 191


>emb|CAA18718.1| putative protein [Arabidopsis thaliana] gi|7268951|emb|CAB81261.1|
            putative protein [Arabidopsis thaliana]
          Length = 1240

 Score =  114 bits (285), Expect = 8e-24
 Identities = 57/84 (67%), Positives = 67/84 (79%), Gaps = 3/84 (3%)
 Frame = -3

Query: 260  FLPHVV---RPKKLLILVNPFGGKKSASKIFTADVKPLLDDANIKYTMQETTHQLHAKEI 90
            F  H+V   RPKKLL+ VNPFGGKK+A KIF  +VKPL +DANI+  +QET +QLHAKEI
Sbjct: 897  FHQHLVSLGRPKKLLVFVNPFGGKKTARKIFQEEVKPLFEDANIQLEIQETKYQLHAKEI 956

Query: 89   ASKMDLSMYDGIVCVSGDGILVEM 18
               MD+S YDGIVCVSGDGILVE+
Sbjct: 957  VRSMDVSKYDGIVCVSGDGILVEV 980



 Score =  110 bits (275), Expect = 1e-22
 Identities = 51/81 (62%), Positives = 65/81 (80%)
 Frame = -3

Query: 260 FLPHVVRPKKLLILVNPFGGKKSASKIFTADVKPLLDDANIKYTMQETTHQLHAKEIASK 81
           +L  + RPK+LL+ VNPFGGKKSA +IF  +VKPL +DA+++  +QET +QLHAKE    
Sbjct: 370 YLDSLGRPKRLLVFVNPFGGKKSAREIFVKEVKPLFEDADVQLEIQETKYQLHAKEFVKS 429

Query: 80  MDLSMYDGIVCVSGDGILVEM 18
           MD+S YDGIVCVSGDGILVE+
Sbjct: 430 MDVSKYDGIVCVSGDGILVEV 450