BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00015319
         (1305 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002281783.1| PREDICTED: vacuole membrane protein 1 [Vitis...   524   e-146
ref|XP_002312524.1| predicted protein [Populus trichocarpa] gi|2...   509   e-142
ref|NP_567450.1| SNARE associated Golgi protein [Arabidopsis tha...   508   e-141
gb|AAL24087.1| unknown protein [Arabidopsis thaliana]                 505   e-141
ref|XP_002516770.1| vacuole membrane protein, putative [Ricinus ...   503   e-140

>ref|XP_002281783.1| PREDICTED: vacuole membrane protein 1 [Vitis vinifera]
            gi|297737027|emb|CBI26228.3| unnamed protein product
            [Vitis vinifera]
          Length = 439

 Score =  524 bits (1350), Expect = e-146
 Identities = 251/390 (64%), Positives = 299/390 (76%)
 Frame = +2

Query: 134  SLSAVSQGIDTIDSGLREKHQRELENXXXXXXXXXXXXFFVLAIIQYVKRTILYLLGHGA 313
            S S+ S  ++   SGLRE+ Q+E+EN            FF+L ++Q+VK+++ YLL  G 
Sbjct: 12   SASSSSGSLEMAISGLRERQQQEMENLTLKTEPFKVLKFFILVVVQHVKQSVAYLLAKGG 71

Query: 314  WXXXXXXXXXXXXXXXMNINGPHEQHIEEVFQYLHFGLWWLALGVASSIGLGSGLHTFVL 493
            W               M I+GPHE+H++E+ QY  FGLWW+ALG+ASSIGLGSGLHTFVL
Sbjct: 72   WVMLLSVVAMALGFVLMAIDGPHEKHVQELHQYFLFGLWWIALGIASSIGLGSGLHTFVL 131

Query: 494  YLGPHIAFFTIKAMECGRVDIKSAPYDTIQLNRRPSWLAKNCSEYGPPMSSSSHGSDIPF 673
            YLGPHIA FTIKAM+CGRVDIKSAPYDTI+L   PSWL K+CSE+GPP+ SS H   +P 
Sbjct: 132  YLGPHIALFTIKAMQCGRVDIKSAPYDTIRLKSGPSWLDKDCSEFGPPVFSSLHALRVPL 191

Query: 674  SSILPQVQLEAILWGIGTALGELPPYFISRAASLSGGKLEALEEFGTSSSEDDGWIAARL 853
            SSILPQVQ+EA+LWGIGTALGELPPYFIS+AASLSG K +A+EE   SS EDDG ++  L
Sbjct: 192  SSILPQVQVEAVLWGIGTALGELPPYFISKAASLSGSKSDAIEELDASSPEDDGVVSTHL 251

Query: 854  NQLKHWFLSHAQYLNFFTILLLASVPNPLFDLAGIMCGQFGIPFWNFFLATMVGKAXXXX 1033
             Q+K WFLSH+Q+LNFFTIL+LASVPNPLFDLAGIMCGQFGIPFW FFLAT +GKA    
Sbjct: 252  KQIKRWFLSHSQHLNFFTILVLASVPNPLFDLAGIMCGQFGIPFWKFFLATFIGKAIIKT 311

Query: 1034 XXXXXXXXSVCNNQLLNWIENELIWVLSLIPGFDSILPNLVMKLRAMKEKYMTTPPHVTS 1213
                    SVCNNQLL+WIENELIWVLSLIPGF S+LPNL+ KL AM++KY+   P V S
Sbjct: 312  HIQTVFIISVCNNQLLDWIENELIWVLSLIPGFASVLPNLISKLNAMRDKYLAPSPPVPS 371

Query: 1214 NIKVKRWDLSVASIWNTVVLLMLVNFFVQI 1303
            N+KVK+WDLS ASIWNTVV LML+NFF +I
Sbjct: 372  NMKVKKWDLSFASIWNTVVWLMLINFFFKI 401


>ref|XP_002312524.1| predicted protein [Populus trichocarpa] gi|222852344|gb|EEE89891.1|
            predicted protein [Populus trichocarpa]
          Length = 413

 Score =  509 bits (1312), Expect = e-142
 Identities = 241/375 (64%), Positives = 283/375 (75%)
 Frame = +2

Query: 179  LREKHQRELENXXXXXXXXXXXXFFVLAIIQYVKRTILYLLGHGAWXXXXXXXXXXXXXX 358
            +RE+HQ+ELEN            FF LA++QY KR++ YLL  G W              
Sbjct: 8    IRERHQQELENLTLTAQPFKTLKFFALAMVQYCKRSVFYLLAKGGWLMLLSTVVAAVGVV 67

Query: 359  XMNINGPHEQHIEEVFQYLHFGLWWLALGVASSIGLGSGLHTFVLYLGPHIAFFTIKAME 538
             + I+ PHE+H+EE+  YL FGLWW+ALGVASSIGLGSGLHTFV+YLGPHIA FTIKAM+
Sbjct: 68   LVTIDSPHEKHVEELSHYLRFGLWWIALGVASSIGLGSGLHTFVMYLGPHIALFTIKAMQ 127

Query: 539  CGRVDIKSAPYDTIQLNRRPSWLAKNCSEYGPPMSSSSHGSDIPFSSILPQVQLEAILWG 718
            CGRVD+KSAPYDTIQL R PSWL ++C EYG P+   SHG  +P SSILPQVQ+EAILWG
Sbjct: 128  CGRVDLKSAPYDTIQLKRGPSWLDRDCHEYGDPVFPLSHGLRVPLSSILPQVQVEAILWG 187

Query: 719  IGTALGELPPYFISRAASLSGGKLEALEEFGTSSSEDDGWIAARLNQLKHWFLSHAQYLN 898
            +GTALGELPPYFISRAA +SG  L+A+EE   SS  D G +AARL  +KHW L+H+Q+LN
Sbjct: 188  VGTALGELPPYFISRAARMSGSNLDAMEELDASSDGDSGIVAARLKAIKHWLLNHSQHLN 247

Query: 899  FFTILLLASVPNPLFDLAGIMCGQFGIPFWNFFLATMVGKAXXXXXXXXXXXXSVCNNQL 1078
            FFTIL+LASVPNPLFDLAGIMCGQFG+PFW FFLAT++GKA            SVCNNQL
Sbjct: 248  FFTILVLASVPNPLFDLAGIMCGQFGVPFWKFFLATLIGKAIVKTHIQTVFIISVCNNQL 307

Query: 1079 LNWIENELIWVLSLIPGFDSILPNLVMKLRAMKEKYMTTPPHVTSNIKVKRWDLSVASIW 1258
            LNWIENELIWVL L+PGFDS LP+L  KL AMKEKYM   P  +++IKVK+WD S   IW
Sbjct: 308  LNWIENELIWVLGLVPGFDSALPDLTAKLHAMKEKYMAPAPPASADIKVKKWDFSFTGIW 367

Query: 1259 NTVVLLMLVNFFVQI 1303
            NTVV LML+NFF +I
Sbjct: 368  NTVVWLMLLNFFFKI 382


>ref|NP_567450.1| SNARE associated Golgi protein [Arabidopsis thaliana]
            gi|53749170|gb|AAU90070.1| At4g14950 [Arabidopsis
            thaliana] gi|332658128|gb|AEE83528.1| SNARE associated
            Golgi protein [Arabidopsis thaliana]
          Length = 416

 Score =  508 bits (1307), Expect = e-141
 Identities = 243/392 (61%), Positives = 292/392 (74%)
 Frame = +2

Query: 128  MGSLSAVSQGIDTIDSGLREKHQRELENXXXXXXXXXXXXFFVLAIIQYVKRTILYLLGH 307
            MGS    S   D   S LREKH++E+EN             FV A IQY+KR+I YLL H
Sbjct: 1    MGSAGVASSSSDVAISALREKHEKEVENLTLTTQPLNTLKLFVEATIQYIKRSISYLLAH 60

Query: 308  GAWXXXXXXXXXXXXXXXMNINGPHEQHIEEVFQYLHFGLWWLALGVASSIGLGSGLHTF 487
            G W               + ++GPH +H+EEV +Y+ +GLWW+ALGVASSIGLGSGLHTF
Sbjct: 61   GGWFILITTLLVVSGGLLVTVDGPHGKHVEEVLEYVRYGLWWIALGVASSIGLGSGLHTF 120

Query: 488  VLYLGPHIAFFTIKAMECGRVDIKSAPYDTIQLNRRPSWLAKNCSEYGPPMSSSSHGSDI 667
            VLYLGPHIA FT+KA  CGRVD+KSAPYDTIQL R PSWL K+CSE+GPP+  S+ GS +
Sbjct: 121  VLYLGPHIALFTLKATLCGRVDLKSAPYDTIQLKRVPSWLDKSCSEFGPPLMISAAGSRV 180

Query: 668  PFSSILPQVQLEAILWGIGTALGELPPYFISRAASLSGGKLEALEEFGTSSSEDDGWIAA 847
            P +SILPQVQLEAILWGIGTALGELPPYFISRAAS+SG  ++ +EE   SS+ED G++A 
Sbjct: 181  PLTSILPQVQLEAILWGIGTALGELPPYFISRAASISGSTVDGMEELDGSSTEDSGFMAT 240

Query: 848  RLNQLKHWFLSHAQYLNFFTILLLASVPNPLFDLAGIMCGQFGIPFWNFFLATMVGKAXX 1027
             LN++K W L+H+Q+LNFFT+L+LASVPNPLFDLAGIMCGQFGIPFW FFLAT++GKA  
Sbjct: 241  HLNRVKRWLLTHSQHLNFFTVLVLASVPNPLFDLAGIMCGQFGIPFWEFFLATLIGKAII 300

Query: 1028 XXXXXXXXXXSVCNNQLLNWIENELIWVLSLIPGFDSILPNLVMKLRAMKEKYMTTPPHV 1207
                       VCNNQLL+W+ENELIW+LS +PG  S+LP L  KL AMKEKY+  P  V
Sbjct: 301  KTHIQTIFIICVCNNQLLDWMENELIWILSHVPGLASMLPGLTAKLHAMKEKYIDAPSPV 360

Query: 1208 TSNIKVKRWDLSVASIWNTVVLLMLVNFFVQI 1303
             S+IKVK+WD S ASIWN +V LML+NFFV+I
Sbjct: 361  PSHIKVKKWDFSFASIWNGIVWLMLLNFFVKI 392


>gb|AAL24087.1| unknown protein [Arabidopsis thaliana]
          Length = 416

 Score =  505 bits (1301), Expect = e-141
 Identities = 243/392 (61%), Positives = 291/392 (74%)
 Frame = +2

Query: 128  MGSLSAVSQGIDTIDSGLREKHQRELENXXXXXXXXXXXXFFVLAIIQYVKRTILYLLGH 307
            MGS    S   D   S LREKH++E+EN             FV A IQY+KR+I YLL H
Sbjct: 1    MGSAGVASSSSDVAISALREKHEKEVENLTLTTQPLNTLKLFVEATIQYIKRSISYLLAH 60

Query: 308  GAWXXXXXXXXXXXXXXXMNINGPHEQHIEEVFQYLHFGLWWLALGVASSIGLGSGLHTF 487
            G W               + ++GPH +H+EEV  Y+ +GLWW+ALGVASSIGLGSGLHTF
Sbjct: 61   GGWFILITTLLVVSGGLLVTVDGPHGKHVEEVLGYVRYGLWWIALGVASSIGLGSGLHTF 120

Query: 488  VLYLGPHIAFFTIKAMECGRVDIKSAPYDTIQLNRRPSWLAKNCSEYGPPMSSSSHGSDI 667
            VLYLGPHIA FT+KA  CGRVD+KSAPYDTIQL R PSWL K+CSE+GPP+  S+ GS +
Sbjct: 121  VLYLGPHIALFTLKATLCGRVDLKSAPYDTIQLKRVPSWLDKSCSEFGPPLMISAAGSRV 180

Query: 668  PFSSILPQVQLEAILWGIGTALGELPPYFISRAASLSGGKLEALEEFGTSSSEDDGWIAA 847
            P +SILPQVQLEAILWGIGTALGELPPYFISRAAS+SG  ++ +EE   SS+ED G++A 
Sbjct: 181  PLTSILPQVQLEAILWGIGTALGELPPYFISRAASISGSTVDGMEELDGSSTEDSGFMAT 240

Query: 848  RLNQLKHWFLSHAQYLNFFTILLLASVPNPLFDLAGIMCGQFGIPFWNFFLATMVGKAXX 1027
             LN++K W L+H+Q+LNFFT+L+LASVPNPLFDLAGIMCGQFGIPFW FFLAT++GKA  
Sbjct: 241  HLNRVKRWLLTHSQHLNFFTVLVLASVPNPLFDLAGIMCGQFGIPFWEFFLATLIGKAII 300

Query: 1028 XXXXXXXXXXSVCNNQLLNWIENELIWVLSLIPGFDSILPNLVMKLRAMKEKYMTTPPHV 1207
                       VCNNQLL+W+ENELIW+LS +PG  S+LP L  KL AMKEKY+  P  V
Sbjct: 301  KTHIQTIFIICVCNNQLLDWMENELIWILSHVPGLASMLPGLTAKLHAMKEKYIDAPSPV 360

Query: 1208 TSNIKVKRWDLSVASIWNTVVLLMLVNFFVQI 1303
             S+IKVK+WD S ASIWN +V LML+NFFV+I
Sbjct: 361  PSHIKVKKWDFSFASIWNGMVWLMLLNFFVKI 392


>ref|XP_002516770.1| vacuole membrane protein, putative [Ricinus communis]
            gi|223543858|gb|EEF45384.1| vacuole membrane protein,
            putative [Ricinus communis]
          Length = 427

 Score =  503 bits (1296), Expect = e-140
 Identities = 242/395 (61%), Positives = 293/395 (74%), Gaps = 2/395 (0%)
 Frame = +2

Query: 125  IMGSLSAVSQGIDTIDS--GLREKHQRELENXXXXXXXXXXXXFFVLAIIQYVKRTILYL 298
            ++ SL  +  G+    S  GL E+ Q+ELEN            FF+LA+IQY K+++LYL
Sbjct: 2    VIFSLLVIQIGLSVFRSQRGLHERQQQELENLTLTTQPLKTLKFFILAMIQYFKKSVLYL 61

Query: 299  LGHGAWXXXXXXXXXXXXXXXMNINGPHEQHIEEVFQYLHFGLWWLALGVASSIGLGSGL 478
            L  G W               + I+GPHE+HI+E+  Y  FGLWW+ALGVASSIGLGSGL
Sbjct: 62   LAKGGWLMLLSIMVAAIGILLVTIDGPHEKHIDELSHYSRFGLWWIALGVASSIGLGSGL 121

Query: 479  HTFVLYLGPHIAFFTIKAMECGRVDIKSAPYDTIQLNRRPSWLAKNCSEYGPPMSSSSHG 658
            HTFVLYLGPHIA FTIKAM+CGRVD+KSA YDTIQL R PSWL K+C E+G P+   SHG
Sbjct: 122  HTFVLYLGPHIALFTIKAMQCGRVDLKSAIYDTIQLKRGPSWLDKDCHEFGDPVFPLSHG 181

Query: 659  SDIPFSSILPQVQLEAILWGIGTALGELPPYFISRAASLSGGKLEALEEFGTSSSEDDGW 838
              +P SSILPQVQ+EA+LWG+GTALGELPPYFISRAAS+SG K++A+EE   SSSED G+
Sbjct: 182  LRVPLSSILPQVQIEAVLWGVGTALGELPPYFISRAASVSGSKVDAMEELNASSSEDSGF 241

Query: 839  IAARLNQLKHWFLSHAQYLNFFTILLLASVPNPLFDLAGIMCGQFGIPFWNFFLATMVGK 1018
            IA  LN +K W LSH+Q+LNFFTIL+LASVPNPLFDLAGIMCGQFGI FW FF AT++GK
Sbjct: 242  IATHLNAIKRWLLSHSQHLNFFTILVLASVPNPLFDLAGIMCGQFGISFWKFFFATLIGK 301

Query: 1019 AXXXXXXXXXXXXSVCNNQLLNWIENELIWVLSLIPGFDSILPNLVMKLRAMKEKYMTTP 1198
            A            SVCNNQLLNW+ENELIWVLS +PG  S+LPN++ KL  +KEKYM   
Sbjct: 302  AIIKTHIQTVFIISVCNNQLLNWMENELIWVLSFVPGLASVLPNIIAKLHTIKEKYMAPA 361

Query: 1199 PHVTSNIKVKRWDLSVASIWNTVVLLMLVNFFVQI 1303
            P V+S+ KVK+WD S A++WNT+V LML+NFF +I
Sbjct: 362  PPVSSSNKVKKWDFSFAALWNTIVWLMLLNFFFKI 396