BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00015301
         (1766 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002263129.2| PREDICTED: aspartic proteinase-like protein ...   619   e-174
ref|XP_004137853.1| PREDICTED: aspartic proteinase-like protein ...   588   e-165
ref|XP_002519417.1| Aspartic proteinase Asp1 precursor, putative...   580   e-163
ref|XP_002326227.1| predicted protein [Populus trichocarpa] gi|2...   571   e-160
ref|XP_003553571.1| PREDICTED: aspartic proteinase-like protein ...   562   e-158

>ref|XP_002263129.2| PREDICTED: aspartic proteinase-like protein 2 isoform 2 [Vitis
            vinifera] gi|297740017|emb|CBI30199.3| unnamed protein
            product [Vitis vinifera]
          Length = 502

 Score =  619 bits (1595), Expect = e-174
 Identities = 301/473 (63%), Positives = 366/473 (77%), Gaps = 4/473 (0%)
 Frame = -3

Query: 1698 GLPATVLPLERAFPANETVELDVLRGLDRARHARILQGVV----DLDVFGASDVYAAGLY 1531
            G PA++L LERAFP N+ VEL+VLR  D+ARH R+L+GVV    D  V+G SD Y  GLY
Sbjct: 27   GSPASLLTLERAFPVNQRVELEVLRARDQARHGRLLRGVVGGVVDFTVYGTSDPYLVGLY 86

Query: 1530 YTKLRLGSPPKEYNVQIDTGSDILWITCSSCSDCPQTSGLGVELNFYDIAXXXXXXXXXX 1351
            +TK++LGSPP+E+NVQIDTGSDILW+TC+SC+DCP+TSGLG+EL+F+D +          
Sbjct: 87   FTKVKLGSPPREFNVQIDTGSDILWVTCNSCNDCPRTSGLGIELSFFDPSSSSTTSLVSC 146

Query: 1350 SNPICNSMVQTNAAGCIAEGNQCSYSFQYGDGSGTAGHYVSDLLYFDTIMGPSVTANSSA 1171
            S+PIC S+VQT AA C  + NQCSYSF YGDGSGT G+YVSD+LYFDT++G S+ ANSSA
Sbjct: 147  SHPICTSLVQTTAAECSPQSNQCSYSFHYGDGSGTTGYYVSDMLYFDTVLGDSLIANSSA 206

Query: 1170 PIVFGCSSYQSGDLTKTDRALDGIIGFGQQDLSVMSQLSARGFTPKVFSHCLKXXXXXXX 991
             IVFGCS+YQSGDLTK D+A+DGI GFGQQDLSV+SQLS+ G TPKVFSHCLK       
Sbjct: 207  SIVFGCSTYQSGDLTKVDKAIDGIFGFGQQDLSVVSQLSSLGITPKVFSHCLKGEGDGGG 266

Query: 990  XXXXGEILDPTIVYSPLVPSQPHYNLYLLSISVGGQLLPVDPAAFATSSNRGTIVDSGTT 811
                GEIL+P I+YSPLVPSQ HYNL L SISV GQLLP+DPA FATS+N+GTIVDSGTT
Sbjct: 267  KLVLGEILEPNIIYSPLVPSQSHYNLNLQSISVNGQLLPIDPAVFATSNNQGTIVDSGTT 326

Query: 810  LTYLVEEAYDPFVDTVTASVSQYTTPIVSKGNQCYAVSSSVNEIFPLVAFNFEGGASIVL 631
            LTYLVE AYDPFV  +TA+VS  TTP++SKGNQCY VS+SV+EIFP V+ NF GGAS+VL
Sbjct: 327  LTYLVETAYDPFVSAITATVSSSTTPVLSKGNQCYLVSTSVDEIFPPVSLNFAGGASMVL 386

Query: 630  KPTNYLVRLGIVDGAPLWCIGFQKSQDQAVTILGDLVLKDKVFVYDLDRQRIGWADYDCX 451
            KP  YL+ LG  DGA +WCIGFQK  +  +TILGDLVLKDK+FVYDL  QRIGWA+YDC 
Sbjct: 387  KPGEYLMHLGFSDGAAMWCIGFQKVAEPGITILGDLVLKDKIFVYDLAHQRIGWANYDCS 446

Query: 450  XXXXXXXXXSKDEYVNAGQLHADSSTRNVRLQLVLISSFQFFLHTLFYAGFIF 292
                      KDE++N+GQL   SS++N+  + +  S     +H L ++GF+F
Sbjct: 447  LSVNVSVTSGKDEFINSGQLSMSSSSQNMLFEPIPRSIKALLIHILVFSGFLF 499


>ref|XP_004137853.1| PREDICTED: aspartic proteinase-like protein 2-like [Cucumis sativus]
            gi|449521209|ref|XP_004167622.1| PREDICTED: aspartic
            proteinase-like protein 2-like [Cucumis sativus]
          Length = 492

 Score =  588 bits (1517), Expect = e-165
 Identities = 290/465 (62%), Positives = 349/465 (75%), Gaps = 6/465 (1%)
 Frame = -3

Query: 1692 PATVLPLERAFPANETVELDVLRGLDRARHARILQGVVDLDVFGASDVYAAGLYYTKLRL 1513
            P  +L L RA P++  V+L+ LR  DR RHARILQGVVD  V G+SD    GLY+TK++L
Sbjct: 26   PVPLLSLYRALPSSSPVQLETLRARDRLRHARILQGVVDFSVEGSSDPLLVGLYFTKVKL 85

Query: 1512 GSPPKEYNVQIDTGSDILWITCSSCSDCPQTSGLGVELNFYDIAXXXXXXXXXXSNPICN 1333
            G+PP E+ VQIDTGSDILW+ C+SC+ CP++SGLG++LNF+D +          S+PICN
Sbjct: 86   GTPPMEFTVQIDTGSDILWVNCNSCNGCPRSSGLGIQLNFFDASSSSSSSLVSCSDPICN 145

Query: 1332 SMVQTNAAGCIAEGNQCSYSFQYGDGSGTAGHYVSDLLYFDTIMGPSVTANSSAPIVFGC 1153
            S  QT A  C+ + NQCSY+FQYGDGSGT+G+YVS+ +YFD +MG S+ ANSSA +VFGC
Sbjct: 146  SAFQTTATQCLTQSNQCSYTFQYGDGSGTSGYYVSESMYFDMVMGQSMIANSSASVVFGC 205

Query: 1152 SSYQSGDLTKTDRALDGIIGFGQQDLSVMSQLSARGFTPKVFSHCLKXXXXXXXXXXXGE 973
            S+YQSGDLTK+D A+DGI GFG  DLSV+SQLSARG TPKVFSHCLK           GE
Sbjct: 206  STYQSGDLTKSDHAIDGIFGFGPGDLSVISQLSARGITPKVFSHCLKGEGNGGGILVLGE 265

Query: 972  ILDPTIVYSPLVPSQPHYNLYLLSISVGGQLLPVDPAAFATSSNRGTIVDSGTTLTYLVE 793
            +L+P IVYSPLVPSQPHYNLYL SISV GQ LP+DP+ FATS NRGTI+DSGTTL YLVE
Sbjct: 266  VLEPGIVYSPLVPSQPHYNLYLQSISVNGQTLPIDPSVFATSINRGTIIDSGTTLAYLVE 325

Query: 792  EAYDPFVDTVTASVSQYTTPIVSKGNQCYAVSSSVNEIFPLVAFNFEGGASIVLKPTNYL 613
            EAY PFV  +TA+VSQ  TP +SKGNQCY VS+SV EIFPLV+ NF G AS+VLKP  YL
Sbjct: 326  EAYTPFVSAITAAVSQSVTPTISKGNQCYLVSTSVGEIFPLVSLNFAGSASMVLKPEEYL 385

Query: 612  VRLGIVDGAPLWCIGFQKSQDQAVTILGDLVLKDKVFVYDLDRQRIGWADYDCXXXXXXX 433
            + LG  DGA LWCIGFQK Q + VTILGDLV+KDK+FVYDL RQRIGWA YDC       
Sbjct: 386  MHLGFYDGAALWCIGFQKVQ-EGVTILGDLVMKDKIFVYDLARQRIGWASYDCSQAVNVS 444

Query: 432  XXXSKDEYVNAGQLHADSSTRNVRLQ------LVLISSFQFFLHT 316
                K+E+VNAGQL   SS+R+  LQ      L +++S   F+H+
Sbjct: 445  VTSGKNEFVNAGQLSVSSSSRDKLLQSLTMEALAMLTSLILFIHS 489


>ref|XP_002519417.1| Aspartic proteinase Asp1 precursor, putative [Ricinus communis]
            gi|223541280|gb|EEF42831.1| Aspartic proteinase Asp1
            precursor, putative [Ricinus communis]
          Length = 494

 Score =  580 bits (1495), Expect = e-163
 Identities = 289/463 (62%), Positives = 352/463 (76%), Gaps = 4/463 (0%)
 Frame = -3

Query: 1689 ATVLPLERAFPANETVELDVLRGLDRARHARILQG----VVDLDVFGASDVYAAGLYYTK 1522
            AT L LERA P N++ EL  LR  D  RHAR+LQG    VVD  V G+SD Y  GLY+T+
Sbjct: 25   ATFLSLERALPLNQSFELAQLRARDHLRHARLLQGFVGGVVDFSVQGSSDPYLVGLYFTR 84

Query: 1521 LRLGSPPKEYNVQIDTGSDILWITCSSCSDCPQTSGLGVELNFYDIAXXXXXXXXXXSNP 1342
            ++LG+PP+E+NVQIDTGSD+LW+TCSSCS+CPQTSGLG++LN++D            S+P
Sbjct: 85   VKLGTPPREFNVQIDTGSDVLWVTCSSCSNCPQTSGLGIQLNYFDTTSSSTARLVPCSHP 144

Query: 1341 ICNSMVQTNAAGCIAEGNQCSYSFQYGDGSGTAGHYVSDLLYFDTIMGPSVTANSSAPIV 1162
            IC S +QT A  C  + NQCSY+FQYGDGSGT+G+YVSD  YFD ++G S+ ANSSA IV
Sbjct: 145  ICTSQIQTTATQCPPQSNQCSYAFQYGDGSGTSGYYVSDTFYFDAVLGESLIANSSAAIV 204

Query: 1161 FGCSSYQSGDLTKTDRALDGIIGFGQQDLSVMSQLSARGFTPKVFSHCLKXXXXXXXXXX 982
            FGCS+YQSGDLTKTD+A+DGI GFGQ +LSV+SQLS+ G TP+VFSHCLK          
Sbjct: 205  FGCSTYQSGDLTKTDKAVDGIFGFGQGELSVISQLSSHGITPRVFSHCLKGEDSGGGILV 264

Query: 981  XGEILDPTIVYSPLVPSQPHYNLYLLSISVGGQLLPVDPAAFATSSNRGTIVDSGTTLTY 802
             GEIL+P IVYSPLVPSQPHYNL L SI+V GQLLP+DPAAFATSSNRGTI+D+GTTL Y
Sbjct: 265  LGEILEPGIVYSPLVPSQPHYNLDLQSIAVSGQLLPIDPAAFATSSNRGTIIDTGTTLAY 324

Query: 801  LVEEAYDPFVDTVTASVSQYTTPIVSKGNQCYAVSSSVNEIFPLVAFNFEGGASIVLKPT 622
            LVEEAYDPFV  +TA+VSQ  TP ++KGNQCY VS+SV+E+FP V+FNF GGA+++LKP 
Sbjct: 325  LVEEAYDPFVSAITAAVSQLATPTINKGNQCYLVSNSVSEVFPPVSFNFAGGATMLLKPE 384

Query: 621  NYLVRLGIVDGAPLWCIGFQKSQDQAVTILGDLVLKDKVFVYDLDRQRIGWADYDCXXXX 442
             YL+ L    GA LWCIGFQK Q   +TILGDLVLKDK+FVYDL  QRIGWA+YDC    
Sbjct: 385  EYLMYLTNYAGAALWCIGFQKIQG-GITILGDLVLKDKIFVYDLAHQRIGWANYDCSSSV 443

Query: 441  XXXXXXSKDEYVNAGQLHADSSTRNVRLQLVLISSFQFFLHTL 313
                  SKD ++NAGQL   SS+++  L+L+ +SS    +H L
Sbjct: 444  NVSVTSSKD-FINAGQLSVSSSSKDNLLKLLPLSSVALLMHIL 485


>ref|XP_002326227.1| predicted protein [Populus trichocarpa] gi|222833420|gb|EEE71897.1|
            predicted protein [Populus trichocarpa]
          Length = 477

 Score =  571 bits (1471), Expect = e-160
 Identities = 292/465 (62%), Positives = 355/465 (76%), Gaps = 6/465 (1%)
 Frame = -3

Query: 1689 ATVLPLERAFPANET-VELDVLRGLDRARHARILQG----VVDLDVFGASDVYAAGLYYT 1525
            A++L LERAFP N   +EL  LR  DR RHAR+LQG    VVD  V G+SD Y  GLY+T
Sbjct: 9    ASLLHLERAFPLNNHGLELHQLRARDRLRHARLLQGFVGGVVDFSVQGSSDPYLVGLYFT 68

Query: 1524 KLRLGSPPKEYNVQIDTGSDILWITCSSCSDCPQTSGLGVELNFYDIAXXXXXXXXXXSN 1345
            K++LGSPP+E+NVQIDTGSD+LW+ C+SC++CP+TSGLG++LNF+D +          S+
Sbjct: 69   KVKLGSPPREFNVQIDTGSDVLWVCCNSCNNCPRTSGLGIQLNFFDSSSSSTAGQVRCSD 128

Query: 1344 PICNSMVQTNAAGCIAEGNQCSYSFQYGDGSGTAGHYVSDLLYFDTIMGPSVTANSSAPI 1165
            PIC S VQT A  C ++ +QCSY+FQYGDGSGT+G+YVSD LYFD I+G S+  NSSA I
Sbjct: 129  PICTSAVQTTATQCSSQTDQCSYTFQYGDGSGTSGYYVSDTLYFDAILGQSLIDNSSALI 188

Query: 1164 VFGCSSYQSGDLTKTDRALDGIIGFGQQDLSVMSQLSARGFTPKVFSHCLKXXXXXXXXX 985
            VFGCS+YQSGDLTKTD+A+DGI GFGQ +LSV+SQLS RG TP+VFSHCLK         
Sbjct: 189  VFGCSAYQSGDLTKTDKAVDGIFGFGQGELSVISQLSTRGITPRVFSHCLKGDGSGGGIL 248

Query: 984  XXGEILDPTIVYSPLVPSQPHYNLYLLSISVGGQLLPVDPAAFATSSNRGTIVDSGTTLT 805
              GEIL+P IVYSPLVPSQPHYNL LLSI+V GQLLP+DPAAFATS+++GTIVDSGTTL 
Sbjct: 249  VLGEILEPGIVYSPLVPSQPHYNLNLLSIAVNGQLLPIDPAAFATSNSQGTIVDSGTTLA 308

Query: 804  YLVEEAYDPFVDTVTASVSQYTTPIVSKGNQCYAVSSSVNEIFPLVAFNFEGGASIVLKP 625
            YLV EAYDPFV  V A VS   TPI SKGNQCY VS+SV+++FPL +FNF GGAS+VLKP
Sbjct: 309  YLVAEAYDPFVSAVNAIVSPSVTPITSKGNQCYLVSTSVSQMFPLASFNFAGGASMVLKP 368

Query: 624  TNYLVRLGIVDGAPLWCIGFQKSQDQAVTILGDLVLKDKVFVYDLDRQRIGWADYDCXXX 445
             +YL+  G   G+ +WCIGFQK   Q VTILGDLVLKDK+FVYDL RQRIGWA+YDC   
Sbjct: 369  EDYLIPFGSSGGSAMWCIGFQKV--QGVTILGDLVLKDKIFVYDLVRQRIGWANYDCSLS 426

Query: 444  XXXXXXXSKDEYVNAGQLHADSSTRNVRL-QLVLISSFQFFLHTL 313
                   SKD ++NAGQL   SS+R++ L +L+ ++   F +H L
Sbjct: 427  VNVSVTSSKD-FINAGQLSVSSSSRDIMLFELLPLTVMVFLMHIL 470


>ref|XP_003553571.1| PREDICTED: aspartic proteinase-like protein 2-like [Glycine max]
          Length = 500

 Score =  562 bits (1449), Expect = e-158
 Identities = 286/473 (60%), Positives = 348/473 (73%), Gaps = 8/473 (1%)
 Frame = -3

Query: 1701 GGLPATVLPLERAFPANETVELDVLRGLDRARHARILQGVV----DLDVFGASDVYAAGL 1534
            GGL  T LPLERA P N+ VEL+ LR  DRARH RILQGVV    D  V G SD Y  GL
Sbjct: 23   GGLAGTFLPLERAIPLNQQVELEALRARDRARHGRILQGVVGGVVDFSVQGTSDPYFVGL 82

Query: 1533 YYTKLRLGSPPKEYNVQIDTGSDILWITCSSCSDCPQTSGLGVELNFYDIAXXXXXXXXX 1354
            Y+TK++LGSP KE+ VQIDTGSDILWI C +CS+CP +SGLG+EL+F+D A         
Sbjct: 83   YFTKVKLGSPAKEFYVQIDTGSDILWINCITCSNCPHSSGLGIELDFFDTAGSSTAALVS 142

Query: 1353 XSNPICNSMVQTNAAGCIAEGNQCSYSFQYGDGSGTAGHYVSDLLYFDTIM-GPSVTANS 1177
              +PIC+  VQT  + C ++ NQCSY+FQYGDGSGT G+YVSD +YFDT++ G SV ANS
Sbjct: 143  CGDPICSYAVQTATSECSSQANQCSYTFQYGDGSGTTGYYVSDTMYFDTVLLGQSVVANS 202

Query: 1176 SAPIVFGCSSYQSGDLTKTDRALDGIIGFGQQDLSVMSQLSARGFTPKVFSHCLKXXXXX 997
            S+ I+FGCS+YQSGDLTKTD+A+DGI GFG   LSV+SQLS+RG TPKVFSHCLK     
Sbjct: 203  SSTIIFGCSTYQSGDLTKTDKAVDGIFGFGPGALSVISQLSSRGVTPKVFSHCLKGGENG 262

Query: 996  XXXXXXGEILDPTIVYSPLVPSQPHYNLYLLSISVGGQLLPVDPAAFATSSNRGTIVDSG 817
                  GEIL+P+IVYSPLVPSQPHYNL L SI+V GQLLP+D   FAT++N+GTIVDSG
Sbjct: 263  GGVLVLGEILEPSIVYSPLVPSQPHYNLNLQSIAVNGQLLPIDSNVFATTNNQGTIVDSG 322

Query: 816  TTLTYLVEEAYDPFVDTVTASVSQYTTPIVSKGNQCYAVSSSVNEIFPLVAFNFEGGASI 637
            TTL YLV+EAY+PFV  +TA+VSQ++ PI+SKGNQCY VS+SV +IFP V+ NF GGAS+
Sbjct: 323  TTLAYLVQEAYNPFVKAITAAVSQFSKPIISKGNQCYLVSNSVGDIFPQVSLNFMGGASM 382

Query: 636  VLKPTNYLVRLGIVDGAPLWCIGFQKSQDQAVTILGDLVLKDKVFVYDLDRQRIGWADYD 457
            VL P +YL+  G +DGA +WCIGFQK  +Q  TILGDLVLKDK+FVYDL  QRIGWADYD
Sbjct: 383  VLNPEHYLMHYGFLDGAAMWCIGFQK-VEQGFTILGDLVLKDKIFVYDLANQRIGWADYD 441

Query: 456  C--XXXXXXXXXXSKDEYV-NAGQLHADSSTRNVRLQLVLISSFQFFLHTLFY 307
            C            SKD Y+ N+GQ+ A  S      +L+ +    F +H + +
Sbjct: 442  CSLSVNVSLATSKSKDAYINNSGQMSASCSHIGTFSKLLAVGIAAFLVHIIVF 494