BLASTX nr result
ID: Bupleurum21_contig00015296
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00015296
(1668 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_004141433.1| PREDICTED: mechanosensitive ion channel prot... 543 e-152
ref|XP_004157404.1| PREDICTED: mechanosensitive ion channel prot... 540 e-151
ref|XP_004141434.1| PREDICTED: mechanosensitive ion channel prot... 524 e-146
ref|XP_004172736.1| PREDICTED: LOW QUALITY PROTEIN: mechanosensi... 513 e-143
ref|XP_004141432.1| PREDICTED: mechanosensitive ion channel prot... 504 e-140
>ref|XP_004141433.1| PREDICTED: mechanosensitive ion channel protein 10-like [Cucumis
sativus]
Length = 721
Score = 543 bits (1398), Expect = e-152
Identities = 292/554 (52%), Positives = 367/554 (66%), Gaps = 32/554 (5%)
Frame = +3
Query: 3 ACLTIDKLHHITIWGLEVWKWCLLVMVIVSGLLFTSWVMHXXXXXXXXXXXXRKKVLYFV 182
A LT++ L + +WGL+VWKWCLL VI+ GL+FT WVM+ +KKVLYFV
Sbjct: 176 ASLTVNPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLYFV 235
Query: 183 HGLKRSARVCLWLTLVLATWLSLF---KHGVRRSASATKALDYITYTIVSLLIGSVXXXX 353
HGLK+S +V LWL+LVLATW SLF H + S K LD +T+T+ S LIG+
Sbjct: 236 HGLKKSVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASFLIGAFLWLI 295
Query: 354 XXXXXXXXASSFHVTTFFDRIQESIFHQYVLQVXXXXXXXXXXXXXXXTSHVSFNRTKKD 533
AS FH+ FFDRIQES+F +VLQ S F +
Sbjct: 296 KTLLLKIVASKFHMNRFFDRIQESLFLHHVLQTLLRPPLVEDE------STAKFRCCRFC 349
Query: 534 TKGREKTKTAVIDMNRLHRMKQEKVSAWTMKMLVDVISNKGLSTISDALDESDFDGNAKK 713
+ ++ + VIDM ++H +K+EKVSAWTMK+LVD +++ +S +S LD+ + A
Sbjct: 350 FESKKPDRKKVIDMGKIHELKREKVSAWTMKVLVDAVTSSEMS-VSQILDDESYRDVA-- 406
Query: 714 TDKEITNEME-AIAAAYHIFKNVAQPDCMYINESDLRRFMLKEEVDIVFPLIDVAEKGQI 890
D +ITNEM+ A AA IFKNVA P +I E DL FM+ EEV++V+P +V + +I
Sbjct: 407 -DGDITNEMKVAKEAAKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPHFEVDKTRKI 465
Query: 891 DKKALTEWVVKVYNGRKTLAHALNDTKTAVKQLNSXXXXXXXXXXXX------------- 1031
D KALT WVVKVY GRKTLAHAL DTKTAVKQLN+
Sbjct: 466 DMKALTNWVVKVYQGRKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWLLLMEIATTKV 525
Query: 1032 ---------------GNTCKTIFEAIIFVFVMHPFDVGDRCVVDGVQMVVEEMNILNTVF 1166
GNTCKT FEA+IFVFVMHPFDVGDRCVVDGVQ++VEEMNIL TVF
Sbjct: 526 LVFLLTQLAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVF 585
Query: 1167 LKFDNEKIYYPNSVLATKAISNFYRSPDMGDSLEFSIDFTTTVEKIGALKEKILKYLERT 1346
LK +NEK+YYPNSVLATK I+N+YRSPDMGD++EFSI F T VE+IGA+KE+I +YLE
Sbjct: 586 LKLNNEKVYYPNSVLATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEEN 645
Query: 1347 PQYWHPNHNLVVKEIENVNKIEMALFFNHTMNFQDFAEKTRRRTELVIEMKKIFDELNIR 1526
PQ+W+PNHN+VVKEIENVNKI++AL+ NHTMNFQD+AEK RRRT+LV+E+K+IF+EL I
Sbjct: 646 PQHWYPNHNVVVKEIENVNKIKIALYTNHTMNFQDWAEKNRRRTKLVMELKRIFEELKIN 705
Query: 1527 YNLLPQEIRMVKSE 1568
YNLLPQ + + E
Sbjct: 706 YNLLPQTVHLFPVE 719
>ref|XP_004157404.1| PREDICTED: mechanosensitive ion channel protein 10-like [Cucumis
sativus]
Length = 710
Score = 540 bits (1392), Expect = e-151
Identities = 290/554 (52%), Positives = 366/554 (66%), Gaps = 32/554 (5%)
Frame = +3
Query: 3 ACLTIDKLHHITIWGLEVWKWCLLVMVIVSGLLFTSWVMHXXXXXXXXXXXXRKKVLYFV 182
A LT++ L + +WGL+VWKWCLL VI+ GL+FT WVM+ +KKVLYFV
Sbjct: 176 ASLTVNPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLYFV 235
Query: 183 HGLKRSARVCLWLTLVLATWLSLF---KHGVRRSASATKALDYITYTIVSLLIGSVXXXX 353
HGLK+S +V LWL+LVLATW SLF H + S K LD +T+T+ S LIG+
Sbjct: 236 HGLKKSVQVTLWLSLVLATWGSLFDRRNHMISSSRITAKILDAVTWTLASFLIGAFLWLI 295
Query: 354 XXXXXXXXASSFHVTTFFDRIQESIFHQYVLQVXXXXXXXXXXXXXXXTSHVSFNRTKKD 533
AS FH+ FFDRIQES+F +VLQ + +
Sbjct: 296 KTLLLKILASKFHMNRFFDRIQESLFLHHVLQTLLRPPL-----------------VEDE 338
Query: 534 TKGREKTKTAVIDMNRLHRMKQEKVSAWTMKMLVDVISNKGLSTISDALDESDFDGNAKK 713
+ + + + VIDM ++H +K+EKVSAWTMK+LVD +++ +S +S LD+ + A
Sbjct: 339 STAKFRYRKKVIDMGKIHELKREKVSAWTMKVLVDAVTSSEMS-VSQILDDESYRDVA-- 395
Query: 714 TDKEITNEME-AIAAAYHIFKNVAQPDCMYINESDLRRFMLKEEVDIVFPLIDVAEKGQI 890
D +ITNEM+ A AA IFKNVA P +I E DL FM+ EEV++V+P +V + +I
Sbjct: 396 -DGDITNEMKVAKEAAKEIFKNVALPGNKFIEERDLLEFMIPEEVNLVWPHFEVDKTRKI 454
Query: 891 DKKALTEWVVKVYNGRKTLAHALNDTKTAVKQLNSXXXXXXXXXXXX------------- 1031
D KALT WVVKVY GRKTLAHAL DTKTAVKQLN+
Sbjct: 455 DMKALTNWVVKVYQGRKTLAHALKDTKTAVKQLNNLITALIIVVTAVIWLLLMEIATTKV 514
Query: 1032 ---------------GNTCKTIFEAIIFVFVMHPFDVGDRCVVDGVQMVVEEMNILNTVF 1166
GNTCKT FEA+IFVFVMHPFDVGDRCVVDGVQ++VEEMNIL TVF
Sbjct: 515 LVFLLTQLAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDRCVVDGVQLLVEEMNILTTVF 574
Query: 1167 LKFDNEKIYYPNSVLATKAISNFYRSPDMGDSLEFSIDFTTTVEKIGALKEKILKYLERT 1346
LK +NEK+YYPNSVLATK I+N+YRSPDMGD++EFSI F T VE+IGA+KE+I +YLE
Sbjct: 575 LKLNNEKVYYPNSVLATKPITNYYRSPDMGDTVEFSIGFETPVERIGAMKEQIKRYLEEN 634
Query: 1347 PQYWHPNHNLVVKEIENVNKIEMALFFNHTMNFQDFAEKTRRRTELVIEMKKIFDELNIR 1526
PQ+W+PNHN+VVKEIENVNKI++AL+ NHTMNFQD+AEK RRRT+LV+E+K+IF+EL I
Sbjct: 635 PQHWYPNHNVVVKEIENVNKIKIALYTNHTMNFQDWAEKNRRRTKLVMELKRIFEELKIN 694
Query: 1527 YNLLPQEIRMVKSE 1568
YNLLPQ + + E
Sbjct: 695 YNLLPQTVHLFPVE 708
>ref|XP_004141434.1| PREDICTED: mechanosensitive ion channel protein 10-like [Cucumis
sativus]
Length = 709
Score = 524 bits (1349), Expect = e-146
Identities = 287/555 (51%), Positives = 364/555 (65%), Gaps = 33/555 (5%)
Frame = +3
Query: 3 ACLTIDKLHHITIWGLEVWKWCLLVMVIVSGLLFTSWVMHXXXXXXXXXXXXRKKVLYFV 182
A LT++ L + +WGL+VWKWCLL VI+ GL+FT WVM+ +KKVLYFV
Sbjct: 165 ASLTVNPLKNRFLWGLKVWKWCLLATVILCGLIFTRWVMNVVVFLIERNFLLKKKVLYFV 224
Query: 183 HGLKRSARVCLWLTLVLATWLSLF---KHGVRRSASATKALDYITYTIVSLLIGSVXXXX 353
HGLK+S +V LWLTLVLATW SLF H V S K LD +T+T+VSLLIG+
Sbjct: 225 HGLKKSVQVTLWLTLVLATWESLFDRRNHMVSNSGINAKVLDLVTWTLVSLLIGAFLWLI 284
Query: 354 XXXXXXXXASSFHVTTFFDRIQESIFHQYVLQVXXXXXXXXXXXXXXXTSHVSFNRTKKD 533
AS FH+ FFDRIQESIFH ++LQ S F +
Sbjct: 285 KTLLLKIVASKFHMNRFFDRIQESIFHHHILQTLLMARTQEDE------SFAEFRCCRFS 338
Query: 534 TKGREKTKTAVIDMNRLHRMKQEKVSAWTMKMLVDVISNKGLSTISDALDESDFDGNAKK 713
+ ++ I++ ++ ++K+EKVSAW MK LVD +++ +S IS LDES + +
Sbjct: 339 FESKKSDCQKAINIEKILQLKREKVSAWKMKTLVDAVTSSEMS-ISKTLDESYRNAD--- 394
Query: 714 TDKEITNEME-AIAAAYHIFKNVAQPDCMYINESDLRRFMLKE-EVDIVFPLIDVAEKGQ 887
D EIT+EM+ A A IFKNVA P +I E DL +FM+ E EV++++P +V + +
Sbjct: 395 -DGEITDEMKVAKQTAKKIFKNVA-PGKKFIEEKDLLKFMIDEAEVNLLWPHFEVDKTKK 452
Query: 888 IDKKALTEWVVKVYNGRKTLAHALNDTKTAVKQLNSXXXXXXXXXXXX------------ 1031
ID K LT WVVKVY GRKTLAHAL DTKTAVKQLN+
Sbjct: 453 IDMKGLTNWVVKVYQGRKTLAHALKDTKTAVKQLNNLVAALIVVVTAVIWLLLMEIATTK 512
Query: 1032 ----------------GNTCKTIFEAIIFVFVMHPFDVGDRCVVDGVQMVVEEMNILNTV 1163
GNTCKT FEA+IFVFVMHPFDVGD CVVDG+Q++VEEMNIL TV
Sbjct: 513 VLVFLLTQLAVAAFMFGNTCKTAFEALIFVFVMHPFDVGDLCVVDGIQLLVEEMNILTTV 572
Query: 1164 FLKFDNEKIYYPNSVLATKAISNFYRSPDMGDSLEFSIDFTTTVEKIGALKEKILKYLER 1343
FLK +NEK+YYPNSVLATK I+N+YRSPDMGD++EFSI FTT +EKIG +KEKI +YLE
Sbjct: 573 FLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTIEFSISFTTPLEKIGVMKEKIKRYLED 632
Query: 1344 TPQYWHPNHNLVVKEIENVNKIEMALFFNHTMNFQDFAEKTRRRTELVIEMKKIFDELNI 1523
PQ+W+PNH++VV+EIENVNKI++AL+ NHTMNFQD+ EK +RRTELV+E+K+IF+EL I
Sbjct: 633 NPQHWYPNHSVVVQEIENVNKIKIALYTNHTMNFQDWTEKNQRRTELVMELKRIFEELKI 692
Query: 1524 RYNLLPQEIRMVKSE 1568
YNLLPQ + + E
Sbjct: 693 NYNLLPQTVHLFPIE 707
>ref|XP_004172736.1| PREDICTED: LOW QUALITY PROTEIN: mechanosensitive ion channel protein
10-like [Cucumis sativus]
Length = 710
Score = 513 bits (1321), Expect = e-143
Identities = 285/556 (51%), Positives = 360/556 (64%), Gaps = 34/556 (6%)
Frame = +3
Query: 3 ACLTIDKLHHITIWGLEVWKWCLLVMVIVSGLLFTSWVMHXXXXXXXXXXXXRKKVLYFV 182
A LT++ L + +WGL+VWKWCLL VI+ GLLFT WVM+ +KKVLYFV
Sbjct: 165 ASLTVNPLKNRFLWGLKVWKWCLLATVILCGLLFTRWVMNVVVFLIEKNFLFKKKVLYFV 224
Query: 183 HGLKRSARVCLWLTLVLATWLSLF---KHGVRRSASATKALDYITYTIVSLLIGSVXXXX 353
HGLK+S +V LWLTLVLATW SLF H V S K LD +T+T+VSLLIG+
Sbjct: 225 HGLKKSVQVTLWLTLVLATWESLFDRRNHMVSNSGINAKVLDLVTWTLVSLLIGAFLWLI 284
Query: 354 XXXXXXXXASSFHVTTFFDRIQESIFHQYVLQVXXXXXXXXXXXXXXXTSHVSFNRTKKD 533
AS FH+ FFDRIQESIFH ++LQ S F +
Sbjct: 285 KTLLLKIVASKFHMNRFFDRIQESIFHHHILQTLLMARTQEDE------SFAEFRCCRFS 338
Query: 534 TKGREKTKTAVIDMNRLHRMKQEKVSAWTMKMLVDVISNKGLSTISDALDESDFDGNAKK 713
+ ++ I++ ++ ++K+EKVSAW MK LVD +++ +S IS LDES + +
Sbjct: 339 FESKKSDCQKAINIEKILQLKREKVSAWKMKTLVDAVTSSEMS-ISKTLDESYRNAD--- 394
Query: 714 TDKEITNEME-AIAAAYHIFKNVAQPDCMYINESDLRRFMLKE-EVDIVFPLIDVAEKGQ 887
D EIT+EM+ A A IFKNVA P +I E DL +FM+ E EV++++P +V + +
Sbjct: 395 -DGEITDEMKVAKQTAKKIFKNVA-PGKKFIEEKDLLKFMIDEAEVNLLWPHFEVDKTKK 452
Query: 888 IDKKALTEWVVKVYNGRKTLAHALNDTKTAVKQLNSXXXXXXXXXXXX------------ 1031
ID K LT WVVKVY GRKTLAHAL DTKTAVKQLN+
Sbjct: 453 IDMKGLTNWVVKVYQGRKTLAHALKDTKTAVKQLNNLVAALIVVVTAVIWLLLMEIATTK 512
Query: 1032 ----------------GNTCKTIFEAIIFVFVMHPFDVGDRCVVDGVQMVVEEMNILNTV 1163
GNTCK FE +IFVFVMHPFDVGD CVVDG+Q++VEEMNIL TV
Sbjct: 513 VLVFLLTQLAVAAFMFGNTCKNTFEGLIFVFVMHPFDVGDLCVVDGIQLLVEEMNILTTV 572
Query: 1164 FLKFDNEKIYYPNSVLATKAISNFYRSPDMGDSLEFSIDFTTTVEKIGALKEKIL-KYLE 1340
FLK +NEK+YYPNSVLATK I+N+YRSPDMGD++EFSI FTT +EKIG +KEK YLE
Sbjct: 573 FLKLNNEKVYYPNSVLATKPITNYYRSPDMGDTIEFSISFTTPLEKIGVMKEKXRGGYLE 632
Query: 1341 RTPQYWHPNHNLVVKEIENVNKIEMALFFNHTMNFQDFAEKTRRRTELVIEMKKIFDELN 1520
PQ+W+PNH++VV+EIENVNKI++AL+ NHTMNFQD+ EK +RRTELV+E+K+IF+EL
Sbjct: 633 DNPQHWYPNHSVVVQEIENVNKIKIALYTNHTMNFQDWTEKNQRRTELVMELKRIFEELK 692
Query: 1521 IRYNLLPQEIRMVKSE 1568
I YNLLPQ + + E
Sbjct: 693 INYNLLPQTVHLFPIE 708
>ref|XP_004141432.1| PREDICTED: mechanosensitive ion channel protein 10-like, partial
[Cucumis sativus]
Length = 720
Score = 504 bits (1299), Expect = e-140
Identities = 274/552 (49%), Positives = 354/552 (64%), Gaps = 30/552 (5%)
Frame = +3
Query: 3 ACLTIDKLHHITIWGLEVWKWCLLVMVIVSGLLFTSWVMHXXXXXXXXXXXXRKKVLYFV 182
A LT+++L + GLE+WKWCLL VI GL+ T W M+ +KKVLYFV
Sbjct: 177 ASLTVNRLKNCFFLGLEIWKWCLLATVIFCGLILTHWAMNVVVSLIEGNFLLKKKVLYFV 236
Query: 183 HGLKRSARVCLWLTLVLATWLSLFKHGVRRSASAT-KALDYITYTIVSLLIGSVXXXXXX 359
HGLK+S +V LWL VL+TW LF RS+ T K LD IT+T+V+LLIGS
Sbjct: 237 HGLKKSVQVTLWLASVLSTWEPLFNQRNHRSSRTTGKILDAITWTLVALLIGSFLWLVKT 296
Query: 360 XXXXXXASSFHVTTFFDRIQESIFHQYVLQVXXXXXXXXXXXXXXXTSHVSFNRTKKDTK 539
AS FH FFDRIQESIFH +VLQ S F+R +
Sbjct: 297 LLLKILASKFHKDRFFDRIQESIFHHHVLQA-----LLGPPLMQEVESAAKFSRCLFSWE 351
Query: 540 GREKTKTAVIDMNRLHRMKQEKVSAWTMKMLVDVISNKGLSTISDALDESDFDGNAKKTD 719
++ +ID ++H +++EKVS+WTMK+LV+ +++ +S IS LDES ++ + D
Sbjct: 352 NKKSDLKKIIDTGKIHHLQREKVSSWTMKVLVEAVTSSAMS-ISQILDESYYNVD----D 406
Query: 720 KEITNEME-AIAAAYHIFKNVAQPDCMYINESDLRRFMLKEEVDIVFPLIDVAEKGQIDK 896
EI +EME A A I +NVA P +I E DL +F++KEE+D+V P +V E +I K
Sbjct: 407 GEIDHEMEIASVVASKILRNVALPGKKFIQEEDLLQFVVKEEIDLVLPHFEVDETKRIGK 466
Query: 897 KALTEWVVKVYNGRKTLAHALNDTKTAVKQLNS--------------------------- 995
KAL +WVVKV+ RKTLAHAL DTKTAVKQLN+
Sbjct: 467 KALKKWVVKVFQERKTLAHALKDTKTAVKQLNNLVTAVVIIVMAVIWLLLMEIATSKVLV 526
Query: 996 -XXXXXXXXXXXXGNTCKTIFEAIIFVFVMHPFDVGDRCVVDGVQMVVEEMNILNTVFLK 1172
GN CKT FEA+IFVFVMHPFDVGDRCVVDGV ++VEEMNIL TVFLK
Sbjct: 527 FLLSQLAVAAFMFGNACKTTFEALIFVFVMHPFDVGDRCVVDGVPLLVEEMNILTTVFLK 586
Query: 1173 FDNEKIYYPNSVLATKAISNFYRSPDMGDSLEFSIDFTTTVEKIGALKEKILKYLERTPQ 1352
+NEK+YYPNSVLATK ISN+YRSPDM ++ EFSI+F T +E+IGA+KEKI +YLE+ PQ
Sbjct: 587 LNNEKVYYPNSVLATKPISNYYRSPDMSETTEFSINFATPLERIGAMKEKIKRYLEKNPQ 646
Query: 1353 YWHPNHNLVVKEIENVNKIEMALFFNHTMNFQDFAEKTRRRTELVIEMKKIFDELNIRYN 1532
+W P+H +VVKEIENVN+I++AL+ HTM++QD+ EK +RR+ELV+E+K+IF+EL I Y
Sbjct: 647 HWRPSHLMVVKEIENVNEIKIALYSTHTMSYQDYGEKMKRRSELVMELKRIFEELKINYT 706
Query: 1533 LLPQEIRMVKSE 1568
LLPQ I + E
Sbjct: 707 LLPQTIHLFPVE 718