BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00015247
         (1931 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002264839.2| PREDICTED: probable serine/threonine-protein...   777   0.0  
emb|CBI36661.3| unnamed protein product [Vitis vinifera]              760   0.0  
ref|XP_003540698.1| PREDICTED: probable serine/threonine-protein...   749   0.0  
ref|NP_191500.2| non-specific serine/threonine protein kinase [A...   741   0.0  
ref|NP_001190135.1| non-specific serine/threonine protein kinase...   741   0.0  

>ref|XP_002264839.2| PREDICTED: probable serine/threonine-protein kinase GCN2-like [Vitis
            vinifera]
          Length = 1244

 Score =  777 bits (2006), Expect = 0.0
 Identities = 384/549 (69%), Positives = 452/549 (82%)
 Frame = -1

Query: 1931 LPSDWVAEFPEQAILLRRLMSPSPSDRPSATELLKQVFPPRMEYELVDNILRKMHTTEDT 1752
            LPS WVAEFPEQA LL+ LMSPSPSDRP ATELL+  FPPRMEYEL+DNILR M T+EDT
Sbjct: 698  LPSSWVAEFPEQASLLQHLMSPSPSDRPFATELLQHAFPPRMEYELLDNILRTMQTSEDT 757

Query: 1751 SIRDKVVNAIFDEDMVGTKGRQENYDTPKLVKNDASSTQYAELDTAIRDQVVDITIEVFK 1572
             + DKVVNAIFD++M+  K  Q ++   +LV +D SS QY + DT +RD V ++T EVF+
Sbjct: 758  GVYDKVVNAIFDKEMLSAKNLQ-HFGRLRLVGDDTSSIQYTDSDTELRDHVSEVTREVFR 816

Query: 1571 LHCAKHLEILPMKLLGGSSQLERKSVKLLTSGGDMIELCQELRLPFVKWVVANQKTAYKR 1392
             HCAK LE++P++LL    Q  R +VKLLT GGDMIELC ELRLPFV WV+ NQK+++KR
Sbjct: 817  HHCAKRLEVVPIRLLDDCPQTIRNTVKLLTHGGDMIELCHELRLPFVNWVITNQKSSFKR 876

Query: 1391 YEVCCVYRRAIGHSPPNRYLQGDFDIVGGAAALTEAEIIKAAMNTIMHCFHPECCDIHLN 1212
            YEV  VYRRAIGHS PNRYLQGDFD++GGA ALTEAE+IK AM+ + H FH   C IHLN
Sbjct: 877  YEVSYVYRRAIGHSAPNRYLQGDFDMIGGATALTEAEVIKVAMDIVTHFFHSNSCGIHLN 936

Query: 1211 HGDLLDALWSWTGIKEDYRHKVAELLSLLGSLRPQSPERKLKWGLIRQQLRQELNLTEAA 1032
            HGDLL+A+WSW GIK ++R KVAELLS++ SLRPQSPERKLKW +IR+QL+QELNL EA 
Sbjct: 937  HGDLLEAIWSWIGIKAEHRQKVAELLSMMSSLRPQSPERKLKWVVIRRQLQQELNLAEAV 996

Query: 1031 VNKLHTVGLRFCGVADQALPRLRGALPADKATRRALDELAELVSYLRVWKIDKIVHIDPL 852
            VN+L TVGLRFCG ADQALPRLRGALPADK TR+ALDELA+L SYLRVW+I+K V ID L
Sbjct: 997  VNRLQTVGLRFCGAADQALPRLRGALPADKPTRKALDELADLFSYLRVWRIEKHVFIDAL 1056

Query: 851  MPPTESYHRNLFFQIFLRKDTNSSSLVEGTLLAVGGRYDHLIHNLWNHVHKSNPPGAVGV 672
            MPPTESYHR+LFFQI+L K+ N  SL EG LLA+GGRYD L+  +     KSNPPGAVGV
Sbjct: 1057 MPPTESYHRDLFFQIYLMKENNPGSLKEGVLLAIGGRYDQLLREMC-FASKSNPPGAVGV 1115

Query: 671  SLALETIIQHCSAELKSFRNDATSSVLICSRGGGGLLKERMELVAELWEENIKAEFVPTS 492
            SLALET+IQH S +++ FRN+   +VL+CSRGGGGLL+ERMELVA LWEENIKAEFVP S
Sbjct: 1116 SLALETVIQHSSMDIRPFRNEVGINVLVCSRGGGGLLEERMELVAGLWEENIKAEFVPVS 1175

Query: 491  DPSLTEQYEYANEHDIRCLVIITEMSVTEKGFVKVRHLELKRAKEIDRENLVKFLLEAMA 312
            DPSLTEQYEYANEHDI+CLVIIT+  V+   FVKVRHLELK+ KE++RENLVKF L ++A
Sbjct: 1176 DPSLTEQYEYANEHDIKCLVIITDTGVSPTDFVKVRHLELKKEKEVERENLVKF-LHSIA 1234

Query: 311  SQFRNPSIW 285
            +QFRN SIW
Sbjct: 1235 TQFRNLSIW 1243


>emb|CBI36661.3| unnamed protein product [Vitis vinifera]
          Length = 859

 Score =  760 bits (1963), Expect = 0.0
 Identities = 374/533 (70%), Positives = 439/533 (82%)
 Frame = -1

Query: 1931 LPSDWVAEFPEQAILLRRLMSPSPSDRPSATELLKQVFPPRMEYELVDNILRKMHTTEDT 1752
            LPS WVAEFPEQA LL+ LMSPSPSDRP ATELL+  FPPRMEYEL+DNILR M T+EDT
Sbjct: 291  LPSSWVAEFPEQASLLQHLMSPSPSDRPFATELLQHAFPPRMEYELLDNILRTMQTSEDT 350

Query: 1751 SIRDKVVNAIFDEDMVGTKGRQENYDTPKLVKNDASSTQYAELDTAIRDQVVDITIEVFK 1572
             + DKVVNAIFD++M+  K  Q ++   +LV +D SS QY + DT +RD V ++T EVF+
Sbjct: 351  GVYDKVVNAIFDKEMLSAKNLQ-HFGRLRLVGDDTSSIQYTDSDTELRDHVSEVTREVFR 409

Query: 1571 LHCAKHLEILPMKLLGGSSQLERKSVKLLTSGGDMIELCQELRLPFVKWVVANQKTAYKR 1392
             HCAK LE++P++LL    Q  R +VKLLT GGDMIELC ELRLPFV WV+ NQK+++KR
Sbjct: 410  HHCAKRLEVVPIRLLDDCPQTIRNTVKLLTHGGDMIELCHELRLPFVNWVITNQKSSFKR 469

Query: 1391 YEVCCVYRRAIGHSPPNRYLQGDFDIVGGAAALTEAEIIKAAMNTIMHCFHPECCDIHLN 1212
            YEV  VYRRAIGHS PNRYLQGDFD++GGA ALTEAE+IK AM+ + H FH   C IHLN
Sbjct: 470  YEVSYVYRRAIGHSAPNRYLQGDFDMIGGATALTEAEVIKVAMDIVTHFFHSNSCGIHLN 529

Query: 1211 HGDLLDALWSWTGIKEDYRHKVAELLSLLGSLRPQSPERKLKWGLIRQQLRQELNLTEAA 1032
            HGDLL+A+WSW GIK ++R KVAELLS++ SLRPQSPERKLKW +IR+QL+QELNL EA 
Sbjct: 530  HGDLLEAIWSWIGIKAEHRQKVAELLSMMSSLRPQSPERKLKWVVIRRQLQQELNLAEAV 589

Query: 1031 VNKLHTVGLRFCGVADQALPRLRGALPADKATRRALDELAELVSYLRVWKIDKIVHIDPL 852
            VN+L TVGLRFCG ADQALPRLRGALPADK TR+ALDELA+L SYLRVW+I+K V ID L
Sbjct: 590  VNRLQTVGLRFCGAADQALPRLRGALPADKPTRKALDELADLFSYLRVWRIEKHVFIDAL 649

Query: 851  MPPTESYHRNLFFQIFLRKDTNSSSLVEGTLLAVGGRYDHLIHNLWNHVHKSNPPGAVGV 672
            MPPTESYHR+LFFQI+L K+ N  SL EG LLA+GGRYD L+  +     KSNPPGAVGV
Sbjct: 650  MPPTESYHRDLFFQIYLMKENNPGSLKEGVLLAIGGRYDQLLREMC-FASKSNPPGAVGV 708

Query: 671  SLALETIIQHCSAELKSFRNDATSSVLICSRGGGGLLKERMELVAELWEENIKAEFVPTS 492
            SLALET+IQH S +++ FRN+   +VL+CSRGGGGLL+ERMELVA LWEENIKAEFVP S
Sbjct: 709  SLALETVIQHSSMDIRPFRNEVGINVLVCSRGGGGLLEERMELVAGLWEENIKAEFVPVS 768

Query: 491  DPSLTEQYEYANEHDIRCLVIITEMSVTEKGFVKVRHLELKRAKEIDRENLVK 333
            DPSLTEQYEYANEHDI+CLVIIT+  V+   FVKVRHLELK+ KE++RENLVK
Sbjct: 769  DPSLTEQYEYANEHDIKCLVIITDTGVSPTDFVKVRHLELKKEKEVERENLVK 821


>ref|XP_003540698.1| PREDICTED: probable serine/threonine-protein kinase GCN2-like
            [Glycine max]
          Length = 1222

 Score =  749 bits (1933), Expect = 0.0
 Identities = 364/549 (66%), Positives = 439/549 (79%)
 Frame = -1

Query: 1931 LPSDWVAEFPEQAILLRRLMSPSPSDRPSATELLKQVFPPRMEYELVDNILRKMHTTEDT 1752
            +P  WV EFPEQ  LLR+LMSP+PSDRPSATELL+  FP RME EL+D+ILR M  +EDT
Sbjct: 683  VPHTWVVEFPEQESLLRQLMSPAPSDRPSATELLQNAFPQRMESELLDDILRTMQKSEDT 742

Query: 1751 SIRDKVVNAIFDEDMVGTKGRQENYDTPKLVKNDASSTQYAELDTAIRDQVVDITIEVFK 1572
            SI DKV+NAIFDE+M+ TK  ++N          +SS QY + +T +RD VVD   E+F+
Sbjct: 743  SIYDKVLNAIFDEEMLSTKHIRQN----------SSSIQYTDFETEVRDYVVDANREIFR 792

Query: 1571 LHCAKHLEILPMKLLGGSSQLERKSVKLLTSGGDMIELCQELRLPFVKWVVANQKTAYKR 1392
             HCAKHLEI  M+LL    Q  R +VKLLT GGDM+ELC ELRLPFV W+++NQK+++KR
Sbjct: 793  QHCAKHLEIPTMRLLDDCPQFNRNAVKLLTHGGDMLELCHELRLPFVNWIISNQKSSFKR 852

Query: 1391 YEVCCVYRRAIGHSPPNRYLQGDFDIVGGAAALTEAEIIKAAMNTIMHCFHPECCDIHLN 1212
            YE+ CV+RRAIGHS PN YLQGDFDI+GG +ALTEAE+IK   + +   FH + CDIHLN
Sbjct: 853  YEISCVFRRAIGHSSPNHYLQGDFDIIGGTSALTEAEVIKVTRDIVTCFFHEDSCDIHLN 912

Query: 1211 HGDLLDALWSWTGIKEDYRHKVAELLSLLGSLRPQSPERKLKWGLIRQQLRQELNLTEAA 1032
            HGDLLDA+WSW G+K ++R KVAELLS++GSLRPQS ERK KW +IR+QL QELNL EA 
Sbjct: 913  HGDLLDAIWSWIGVKVEHRLKVAELLSMMGSLRPQSSERKSKWVVIRRQLLQELNLAEAM 972

Query: 1031 VNKLHTVGLRFCGVADQALPRLRGALPADKATRRALDELAELVSYLRVWKIDKIVHIDPL 852
            VN+L TVGLRFCG ADQALPRLRGALP+DK   +ALDEL+ELVS LR+W+IDK ++ID L
Sbjct: 973  VNRLQTVGLRFCGSADQALPRLRGALPSDKRAFKALDELSELVSLLRIWRIDKNIYIDAL 1032

Query: 851  MPPTESYHRNLFFQIFLRKDTNSSSLVEGTLLAVGGRYDHLIHNLWNHVHKSNPPGAVGV 672
            MPPTESYHR+LFFQ++LRK+ +  SL EG LLAVGGRYD+L H LW+  +K NPP  VG 
Sbjct: 1033 MPPTESYHRDLFFQVYLRKENSPGSLSEGALLAVGGRYDYLFHQLWSSDYKGNPPTGVGT 1092

Query: 671  SLALETIIQHCSAELKSFRNDATSSVLICSRGGGGLLKERMELVAELWEENIKAEFVPTS 492
            SLALETIIQ+C  + K  RN+A+ ++L+CSRGGGGLL ERMELVAELWEEN KAEFVPT 
Sbjct: 1093 SLALETIIQNCPVDFKPNRNEASINILVCSRGGGGLLVERMELVAELWEENFKAEFVPTP 1152

Query: 491  DPSLTEQYEYANEHDIRCLVIITEMSVTEKGFVKVRHLELKRAKEIDRENLVKFLLEAMA 312
            DPSLTEQYEYANEH I+CLVIIT+   +  G VKVRHLE KR K ++++NLVKFL +AMA
Sbjct: 1153 DPSLTEQYEYANEHGIKCLVIITDTDFSLTGSVKVRHLEHKREKNVEKKNLVKFLSDAMA 1212

Query: 311  SQFRNPSIW 285
            +QFRNPSIW
Sbjct: 1213 TQFRNPSIW 1221


>ref|NP_191500.2| non-specific serine/threonine protein kinase [Arabidopsis thaliana]
            gi|68052253|sp|Q9LX30.2|GCN2_ARATH RecName: Full=Probable
            serine/threonine-protein kinase GCN2
            gi|24940154|emb|CAD30860.1| GCN2 homologue [Arabidopsis
            thaliana] gi|332646397|gb|AEE79918.1| non-specific
            serine/threonine protein kinase [Arabidopsis thaliana]
          Length = 1241

 Score =  741 bits (1914), Expect = 0.0
 Identities = 369/552 (66%), Positives = 442/552 (80%), Gaps = 2/552 (0%)
 Frame = -1

Query: 1931 LPSDWVAEFPEQAILLRRLMSPSPSDRPSATELLKQVFPPRMEYELVDNILRKMHTTEDT 1752
            LP  WV EFPEQA LLRRLMSPSPSDRPSATELLK  FPPRME EL+DNILR M T+ED+
Sbjct: 693  LPLKWVNEFPEQASLLRRLMSPSPSDRPSATELLKHAFPPRMESELLDNILRIMQTSEDS 752

Query: 1751 SIRDKVVNAIFDEDMVGTKGRQENYDTPKLVKNDASSTQYAELDTAIRDQVVDITIEVFK 1572
            S+ D+VV+ IFDE+++  K  Q +    +L  +D S  QY E++T +RD VV+IT EVF+
Sbjct: 753  SVYDRVVSVIFDEEVLEMKSHQSSRS--RLCADD-SYIQYTEINTELRDYVVEITKEVFR 809

Query: 1571 LHCAKHLEILPMKLLGGSSQLERKSVKLLTSGGDMIELCQELRLPFVKWVVANQKTAYKR 1392
             HCAKHLE++PM+LL    Q  RK+VKLLT+GGDM+ELC ELRLPFV W+  NQK+++KR
Sbjct: 810  QHCAKHLEVIPMRLLSDCPQFSRKTVKLLTNGGDMLELCYELRLPFVHWISVNQKSSFKR 869

Query: 1391 YEVCCVYRRAIGHSPPNRYLQGDFDIVGGAAALTEAEIIKAAMNTIMHCFHPECCDIHLN 1212
            YE+  VYRRAIGHSPPN  LQ DFDIVGG  +LTEAE++K  ++   H FH   CDIHLN
Sbjct: 870  YEISHVYRRAIGHSPPNPCLQADFDIVGGTLSLTEAEVLKVIVDITTHIFHRGSCDIHLN 929

Query: 1211 HGDLLDALWSWTGIKEDYRHKVAELLSLLGSLRPQSPERKLKWGLIRQQLRQELNLTEAA 1032
            HGDLLDA+WSW GIK ++R KVAELLS++GSLRPQS ERKLKW  IR+QL QEL L EA 
Sbjct: 930  HGDLLDAIWSWAGIKAEHRRKVAELLSMMGSLRPQSSERKLKWVFIRRQLLQELKLPEAV 989

Query: 1031 VNKLHTVGLRFCGVADQALPRLRGALPADKATRRALDELAELVSYLRVWKIDKIVHIDPL 852
            VN+L TV  RFCG ADQALPRLRGAL AD+ TR+ALDEL+ L++YLRVW+I++ VHID L
Sbjct: 990  VNRLQTVASRFCGDADQALPRLRGALRADRPTRKALDELSNLLTYLRVWRIEEHVHIDVL 1049

Query: 851  MPPTESYHRNLFFQIFLRKDTNSSSLVEGTLLAVGGRYDHLIHNLWNHVHKSNPPGAVGV 672
            MPPTESYHRNLFFQ+FL K+ +S +  +G LLAVGGRYD L+  + +  HK N PGAVGV
Sbjct: 1050 MPPTESYHRNLFFQVFLTKENSSGTSNDGVLLAVGGRYDWLVQEVCDREHKMNLPGAVGV 1109

Query: 671  SLALETIIQHCSAELKSFRNDATSSVLICSRGGGGLLKERMELVAELWEENIKAEFVPTS 492
            SLALETI QH   +L+  RN+ ++SVL+CSRGGGGLL +RMELVAELWE++IKAEFVPT 
Sbjct: 1110 SLALETIFQHLPMDLRPIRNEVSTSVLVCSRGGGGLLVQRMELVAELWEKSIKAEFVPTP 1169

Query: 491  DPSLTEQYEYANEHDIRCLVIITEMSVTEK--GFVKVRHLELKRAKEIDRENLVKFLLEA 318
            DPSLTEQYEYANEH+I+CLVIITE  V +    FVKVRHLELK+ K + RE LVKFLL+A
Sbjct: 1170 DPSLTEQYEYANEHEIKCLVIITESGVAQNQIEFVKVRHLELKKEKVVGREELVKFLLDA 1229

Query: 317  MASQFRNPSIWT 282
            MA QFRNPS+W+
Sbjct: 1230 MAVQFRNPSVWS 1241


>ref|NP_001190135.1| non-specific serine/threonine protein kinase [Arabidopsis thaliana]
            gi|332646398|gb|AEE79919.1| non-specific serine/threonine
            protein kinase [Arabidopsis thaliana]
          Length = 1265

 Score =  741 bits (1914), Expect = 0.0
 Identities = 369/552 (66%), Positives = 442/552 (80%), Gaps = 2/552 (0%)
 Frame = -1

Query: 1931 LPSDWVAEFPEQAILLRRLMSPSPSDRPSATELLKQVFPPRMEYELVDNILRKMHTTEDT 1752
            LP  WV EFPEQA LLRRLMSPSPSDRPSATELLK  FPPRME EL+DNILR M T+ED+
Sbjct: 717  LPLKWVNEFPEQASLLRRLMSPSPSDRPSATELLKHAFPPRMESELLDNILRIMQTSEDS 776

Query: 1751 SIRDKVVNAIFDEDMVGTKGRQENYDTPKLVKNDASSTQYAELDTAIRDQVVDITIEVFK 1572
            S+ D+VV+ IFDE+++  K  Q +    +L  +D S  QY E++T +RD VV+IT EVF+
Sbjct: 777  SVYDRVVSVIFDEEVLEMKSHQSSRS--RLCADD-SYIQYTEINTELRDYVVEITKEVFR 833

Query: 1571 LHCAKHLEILPMKLLGGSSQLERKSVKLLTSGGDMIELCQELRLPFVKWVVANQKTAYKR 1392
             HCAKHLE++PM+LL    Q  RK+VKLLT+GGDM+ELC ELRLPFV W+  NQK+++KR
Sbjct: 834  QHCAKHLEVIPMRLLSDCPQFSRKTVKLLTNGGDMLELCYELRLPFVHWISVNQKSSFKR 893

Query: 1391 YEVCCVYRRAIGHSPPNRYLQGDFDIVGGAAALTEAEIIKAAMNTIMHCFHPECCDIHLN 1212
            YE+  VYRRAIGHSPPN  LQ DFDIVGG  +LTEAE++K  ++   H FH   CDIHLN
Sbjct: 894  YEISHVYRRAIGHSPPNPCLQADFDIVGGTLSLTEAEVLKVIVDITTHIFHRGSCDIHLN 953

Query: 1211 HGDLLDALWSWTGIKEDYRHKVAELLSLLGSLRPQSPERKLKWGLIRQQLRQELNLTEAA 1032
            HGDLLDA+WSW GIK ++R KVAELLS++GSLRPQS ERKLKW  IR+QL QEL L EA 
Sbjct: 954  HGDLLDAIWSWAGIKAEHRRKVAELLSMMGSLRPQSSERKLKWVFIRRQLLQELKLPEAV 1013

Query: 1031 VNKLHTVGLRFCGVADQALPRLRGALPADKATRRALDELAELVSYLRVWKIDKIVHIDPL 852
            VN+L TV  RFCG ADQALPRLRGAL AD+ TR+ALDEL+ L++YLRVW+I++ VHID L
Sbjct: 1014 VNRLQTVASRFCGDADQALPRLRGALRADRPTRKALDELSNLLTYLRVWRIEEHVHIDVL 1073

Query: 851  MPPTESYHRNLFFQIFLRKDTNSSSLVEGTLLAVGGRYDHLIHNLWNHVHKSNPPGAVGV 672
            MPPTESYHRNLFFQ+FL K+ +S +  +G LLAVGGRYD L+  + +  HK N PGAVGV
Sbjct: 1074 MPPTESYHRNLFFQVFLTKENSSGTSNDGVLLAVGGRYDWLVQEVCDREHKMNLPGAVGV 1133

Query: 671  SLALETIIQHCSAELKSFRNDATSSVLICSRGGGGLLKERMELVAELWEENIKAEFVPTS 492
            SLALETI QH   +L+  RN+ ++SVL+CSRGGGGLL +RMELVAELWE++IKAEFVPT 
Sbjct: 1134 SLALETIFQHLPMDLRPIRNEVSTSVLVCSRGGGGLLVQRMELVAELWEKSIKAEFVPTP 1193

Query: 491  DPSLTEQYEYANEHDIRCLVIITEMSVTEK--GFVKVRHLELKRAKEIDRENLVKFLLEA 318
            DPSLTEQYEYANEH+I+CLVIITE  V +    FVKVRHLELK+ K + RE LVKFLL+A
Sbjct: 1194 DPSLTEQYEYANEHEIKCLVIITESGVAQNQIEFVKVRHLELKKEKVVGREELVKFLLDA 1253

Query: 317  MASQFRNPSIWT 282
            MA QFRNPS+W+
Sbjct: 1254 MAVQFRNPSVWS 1265