BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00015237
         (1417 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003635424.1| PREDICTED: LOW QUALITY PROTEIN: putative rib...   130   2e-54
ref|XP_002522452.1| nucleic acid binding protein, putative [Rici...   137   3e-45
ref|NP_194638.1| Ribonuclease H-like protein [Arabidopsis thalia...   100   1e-35
gb|ABN08723.1| Polynucleotidyl transferase, Ribonuclease H fold ...   101   5e-32
gb|AAG13524.1|AC068924_29 putative non-LTR retroelement reverse ...   114   2e-31

>ref|XP_003635424.1| PREDICTED: LOW QUALITY PROTEIN: putative ribonuclease H protein
           At1g65750-like [Vitis vinifera]
          Length = 820

 Score =  130 bits (328), Expect(3) = 2e-54
 Identities = 77/207 (37%), Positives = 102/207 (49%), Gaps = 5/207 (2%)
 Frame = +1

Query: 7   AKLGRNPSYVWRSILESQTLLKRAARWRIGDGRSISVMDQPWLPESQNPFVTSQHLA-LQ 183
           A+LG NPSY+WRSIL +Q LLKR   W I  G  + V    WLP+S N  + +  +A   
Sbjct: 366 AELGSNPSYMWRSILAAQGLLKRGCYWSIASGTKVQVWGDSWLPDSSNRLIITPPVAGFD 425

Query: 184 GVTVYNLFKTNSREWDEDIIKELCNDRDRMSIYDIVLCRNQYIDTRYWYYEQNGNYTVKS 363
           G+ V  L       W ED I++    RD   I  I L  +   D   W ++  G YT +S
Sbjct: 426 GIKVDELITEGL--WREDFIRDKFMARDADLILSIPLPMSSREDQISWSFDARGEYTARS 483

Query: 364 AYKLLQSFYGRW----EENDEVKLWRSLWKLQVPAKVKVFLWRALNNCLPTKCNLQTRMV 531
            Y  L+ F         + D   +W  LWK+  P K+  F WRA  NCLPT+  L  R V
Sbjct: 484 GYGALRCFRQSTALVASDVDSNFVWAQLWKVTAPPKILNFAWRAARNCLPTRFALTIRHV 543

Query: 532 QTDDVCPVCGMDKETTCIPLLYSLFVC 612
            T   CP+C  + ETT    L++L  C
Sbjct: 544 DTPMCCPICRSELETT----LHALVEC 566



 Score = 67.4 bits (163), Expect(3) = 2e-54
 Identities = 37/128 (28%), Positives = 54/128 (42%), Gaps = 4/128 (3%)
 Frame = +2

Query: 587 HCFIHCLFAKSCWH---IVCPESNFNSTMNFSMWFLYMLEHHKEKAAAISM-VCWSIWRA 754
           H  + C+ A+  W    +   + NF S   F  W   M  +      A  + VCW +W  
Sbjct: 561 HALVECVAARDVWDESGLAMLQGNFGS---FVDWLATMFAYCDFVVFAKYLAVCWGLWWR 617

Query: 755 RNDVVWNNTNWRAGNVVTSAVAYLTQWNDVQNLGKATSPTPAHSTGDTEQWNKPEIGCVK 934
           RNDVVWN   W +  VV      L  W             P++S+    +W KP+ G +K
Sbjct: 618 RNDVVWNGRIWHSQQVVNGCFTMLESWFHANETLATAVTVPSYSS----KWQKPDYGWIK 673

Query: 935 VNCDATIF 958
           +N D  +F
Sbjct: 674 INVDGAVF 681



 Score = 63.5 bits (153), Expect(3) = 2e-54
 Identities = 36/117 (30%), Positives = 59/117 (50%)
 Frame = +1

Query: 994  RNDKGELLFAARSCFPGKPEVHHAEAIGIREALSWIKAELDKNQGNTVCNLQHRVLVESD 1173
            R+ +G  +      FP +      EA+G+RE LSWI               + R++VE+D
Sbjct: 692  RDHQGRFMGGFAKPFPHQTLPKVVEALGVREVLSWIHE-----------RSRSRIVVETD 740

Query: 1174 CQVAVNAVLKKELICSPFGGVIDECRSILESYNNIDIVFVKRSGNRAADWLASSSCS 1344
            C   V A+  K    + FG +I +C  +L+   ++ +V+ +RS N AA  LA+ +CS
Sbjct: 741  CLRVVQAIQHKSCPNTSFGFIIVDCLDVLQHLVDVQVVYARRSANSAAHCLANGACS 797


>ref|XP_002522452.1| nucleic acid binding protein, putative [Ricinus communis]
           gi|223538337|gb|EEF39944.1| nucleic acid binding
           protein, putative [Ricinus communis]
          Length = 483

 Score =  137 bits (344), Expect(3) = 3e-45
 Identities = 72/203 (35%), Positives = 114/203 (56%), Gaps = 3/203 (1%)
 Frame = +1

Query: 13  LGRNPSYVWRSILESQTLLKRAARWRIGDGRSISVMDQPWLPESQNPFVTSQ---HLALQ 183
           LG NPSY+W S+ + + L+ +  +W+IG G  +S+    WL ++ N F+ +     L LQ
Sbjct: 47  LGTNPSYIWASLQQVRDLICKGVQWKIGKGTEVSIGLHEWLLDASNGFIETNLPDELRLQ 106

Query: 184 GVTVYNLFKTNSREWDEDIIKELCNDRDRMSIYDIVLCRNQYIDTRYWYYEQNGNYTVKS 363
            V   +L K  + +WD++I+ +L ++RD+  I  IVL      D  +W+ +  G ++VK 
Sbjct: 107 PVC--SLLKPGTCDWDQEILNDLFSERDKNLINSIVLSPTVGTDQLFWFKDPKGKFSVKD 164

Query: 364 AYKLLQSFYGRWEENDEVKLWRSLWKLQVPAKVKVFLWRALNNCLPTKCNLQTRMVQTDD 543
            Y++ Q  +     ++ V +WR LWKL + AK KVF+WRAL N LP + NL  R V  D 
Sbjct: 165 VYRVQQPDFSVLYPSNVV-VWRRLWKLNIAAKCKVFMWRALTNRLPVRTNLVMRKVTEDS 223

Query: 544 VCPVCGMDKETTCIPLLYSLFVC 612
            CP C    ET    +++ L +C
Sbjct: 224 SCPCCVSQPET----IMHILVLC 242



 Score = 52.4 bits (124), Expect(3) = 3e-45
 Identities = 39/131 (29%), Positives = 52/131 (39%), Gaps = 9/131 (6%)
 Frame = +2

Query: 587 HCFIHCLFAKSCWHIVCPESNFNSTMNFSMWFLYMLEHHKEKAAAISMVCW-SIWRARND 763
           H  + C      W  V      +   N       + EH  +   A  +V W S+W   ND
Sbjct: 237 HILVLCDVTTQSWKYVNLYQFLSQVSNLLEGARSVFEHFDDSIVASFVVLWWSLWTNMND 296

Query: 764 VVWN--NTNWRAGNVVTSAVAYLTQW------NDVQNLGKATSPTPAHSTGDTEQWNKPE 919
           VVWN    +WRA  V + A ++L QW       D   LG+       H  G    W KP 
Sbjct: 297 VVWNGKKLSWRA--VASRASSFLFQWGKARKLTDYHQLGR-------HFAGGDCLWQKPA 347

Query: 920 IGCVKVNCDAT 952
            G  K+N DA+
Sbjct: 348 TGKYKLNVDAS 358



 Score = 41.2 bits (95), Expect(3) = 3e-45
 Identities = 29/96 (30%), Positives = 52/96 (54%), Gaps = 1/96 (1%)
 Frame = +1

Query: 1063 AEAIGIREALSWIKAE-LDKNQGNTVCNLQHRVLVESDCQVAVNAVLKKELICSPFGGVI 1239
            AEA  ++EALSWI A+ +++ Q  T C L++  L+E +            L+C     ++
Sbjct: 396  AEAWALKEALSWIHAKGMEEVQIETDC-LRNIELLEEELHP------NSYLLC-----LL 443

Query: 1240 DECRSILESYNNIDIVFVKRSGNRAADWLASSSCSS 1347
             +C+ +L   N  ++VFV  S N  A  ++ ++CS+
Sbjct: 444  KDCQDLLRVLNRCNLVFVYGSANTVAHMISKATCSA 479


>ref|NP_194638.1| Ribonuclease H-like protein [Arabidopsis thaliana]
           gi|4972055|emb|CAB43923.1| putative protein [Arabidopsis
           thaliana] gi|7269807|emb|CAB79667.1| putative protein
           [Arabidopsis thaliana] gi|67633766|gb|AAY78807.1|
           putative reverse transcriptase/RNA-dependent DNA
           polymerase [Arabidopsis thaliana]
           gi|332660185|gb|AEE85585.1| Ribonuclease H-like protein
           [Arabidopsis thaliana]
          Length = 575

 Score =  100 bits (249), Expect(3) = 1e-35
 Identities = 64/208 (30%), Positives = 99/208 (47%), Gaps = 18/208 (8%)
 Frame = +1

Query: 7   AKLGRNPSYVWRSILESQTLLKRAARWRIGDGRSISVMDQPWLPESQNPFVTSQHLALQG 186
           A LG  PS+VW+SI  SQ +L++ AR  +G+G  I +    WL         S  L +Q 
Sbjct: 101 APLGSRPSFVWKSIHASQEILRQGARAVVGNGEDIIIWRHKWLDSKP----ASAALRMQR 156

Query: 187 VT------------VYNLFKTNSREWDEDIIKELCNDRDRMSIYDIVLCRNQYIDTRYWY 330
           V             V +L   + REW +D+I+ L  + +R  I ++     + +D+  W 
Sbjct: 157 VPPQEYASVSSILKVSDLIDESGREWRKDVIEMLFPEVERKLIGELRPGGRRILDSYTWD 216

Query: 331 YEQNGNYTVKSAYKLLQSFYGRWEENDEVK------LWRSLWKLQVPAKVKVFLWRALNN 492
           Y  +G+YTVKS Y +L     +     EV       +++ +WK Q   K++ FLW+ L+N
Sbjct: 217 YTSSGDYTVKSGYWVLTQIINKRSSPQEVSEPSLNPIYQKIWKSQTSPKIQHFLWKCLSN 276

Query: 493 CLPTKCNLQTRMVQTDDVCPVCGMDKET 576
            LP    L  R +  +  C  C   KET
Sbjct: 277 SLPVAGALAYRHLSKESACIRCPSCKET 304



 Score = 51.2 bits (121), Expect(3) = 1e-35
 Identities = 31/149 (20%), Positives = 69/149 (46%), Gaps = 8/149 (5%)
 Frame = +2

Query: 572 RQHASHCFIHCLFAKSCWHI----VCPESNFNSTMNFSMWFLYMLEHHK---EKAAA-IS 727
           ++  +H    C FA+  W I    +     +  ++  ++++++ L +     EKA+  + 
Sbjct: 302 KETVNHLLFKCTFARLTWAISSIPIPLGGEWADSIYVNLYWVFNLGNGNPQWEKASQLVP 361

Query: 728 MVCWSIWRARNDVVWNNTNWRAGNVVTSAVAYLTQWNDVQNLGKATSPTPAHSTGDTEQW 907
            + W +W+ RN++V+    + A  V+  A   L +W  ++   ++    P  +     +W
Sbjct: 362 WLLWRLWKNRNELVFRGREFNAQEVLRRAEDDLEEWR-IRTEAESCGTKPQVNRSSCGRW 420

Query: 908 NKPEIGCVKVNCDATIFKSSSQFSVGWIV 994
             P    VK N DAT  + + +  +GW++
Sbjct: 421 RPPPHQWVKCNTDATWNRDNERCGIGWVL 449



 Score = 46.6 bits (109), Expect(3) = 1e-35
 Identities = 36/125 (28%), Positives = 55/125 (44%), Gaps = 2/125 (1%)
 Frame = +1

Query: 976  QCGLD--SRNDKGELLFAARSCFPGKPEVHHAEAIGIREALSWIKAELDKNQGNTVCNLQ 1149
            +CG+    RN+KGE+ +      P    V  AE     EA+ W    L + Q N V    
Sbjct: 442  RCGIGWVLRNEKGEVKWMGARALPKLKSVLEAEL----EAMRWAVLSLSRFQYNYV---- 493

Query: 1150 HRVLVESDCQVAVNAVLKKELICSPFGGVIDECRSILESYNNIDIVFVKRSGNRAADWLA 1329
               + ESD QV +  +L  + I       I + + +L  +  +  VF+ R GN  A+ +A
Sbjct: 494  ---IFESDSQVLIE-ILNNDEIWPSLKPTIQDLQRLLSQFTEVKFVFIPREGNTLAERVA 549

Query: 1330 SSSCS 1344
              S S
Sbjct: 550  RESLS 554


>gb|ABN08723.1| Polynucleotidyl transferase, Ribonuclease H fold [Medicago
           truncatula]
          Length = 355

 Score =  101 bits (252), Expect(3) = 5e-32
 Identities = 52/155 (33%), Positives = 76/155 (49%), Gaps = 2/155 (1%)
 Frame = +1

Query: 91  IGDGRSISVMDQPWLPE--SQNPFVTSQHLALQGVTVYNLFKTNSREWDEDIIKELCNDR 264
           IGDG  I++   PW+    S  P  T      + +TV  L       W++ I+  + N +
Sbjct: 3   IGDGSKINLWTMPWIRNLPSHKPS-TPMPAHHEDLTVNYLLNLEGLSWNQQIVTSIFNPQ 61

Query: 265 DRMSIYDIVLCRNQYIDTRYWYYEQNGNYTVKSAYKLLQSFYGRWEENDEVKLWRSLWKL 444
           D  +I  I L      DTR W     GNYTVKSAY++        +       W ++W L
Sbjct: 62  DAAAILSIPLHNRLLSDTRIWKTTTTGNYTVKSAYRICSDLLHPHDSAHNNSSWMNIWNL 121

Query: 445 QVPAKVKVFLWRALNNCLPTKCNLQTRMVQTDDVC 549
            +P +V+ FLWR  +NCLPT+ NL  R +Q ++ C
Sbjct: 122 NIPHRVRAFLWRLAHNCLPTRVNLNARGIQCEESC 156



 Score = 61.6 bits (148), Expect(3) = 5e-32
 Identities = 41/137 (29%), Positives = 69/137 (50%), Gaps = 9/137 (6%)
 Frame = +2

Query: 584 SHCFIHCLFAKSCW-----HIVCPE----SNFNSTMNFSMWFLYMLEHHKEKAAAISMVC 736
           +H F  C  A  CW     H+V  E    +N  +T+ F   F++ L   +++AA   M+ 
Sbjct: 167 THLFFVCAKAMDCWKNIGLHVVLSELLPLANNFTTLLFD--FMHRLCSQQQQAAG--MLL 222

Query: 737 WSIWRARNDVVWNNTNWRAGNVVTSAVAYLTQWNDVQNLGKATSPTPAHSTGDTEQWNKP 916
           WS+W++RN  +W++T+      ++ A   L +W+ +Q+  K     P H       W KP
Sbjct: 223 WSLWKSRNIKLWDSTDTTTAITISRAKDTLHEWSCMQS-AKTQPINPNHHI----SWVKP 277

Query: 917 EIGCVKVNCDATIFKSS 967
             G +K N DA IF+++
Sbjct: 278 TGGMIKCNMDAAIFQNN 294



 Score = 22.7 bits (47), Expect(3) = 5e-32
 Identities = 13/37 (35%), Positives = 18/37 (48%)
 Frame = +1

Query: 982  GLDSRNDKGELLFAARSCFPGKPEVHHAEAIGIREAL 1092
            G+  RN + ELL    +       V  AEAI + EA+
Sbjct: 300  GICCRNSEDELLLGKSAIIHSSLSVLEAEAIVLLEAM 336


>gb|AAG13524.1|AC068924_29 putative non-LTR retroelement reverse transcriptase [Oryza sativa
            Japonica Group]
          Length = 1382

 Score =  114 bits (286), Expect(2) = 2e-31
 Identities = 62/194 (31%), Positives = 98/194 (50%), Gaps = 11/194 (5%)
 Frame = +1

Query: 19   RNPSYVWRSILESQTLLKRAARWRIGDGRSISVMDQPWLPESQNPFVTSQHLALQGVTVY 198
            ++PS+ WRS+L  + LL +  RW +GDG++I +    W+P  +   VT+        TV 
Sbjct: 917  KSPSFTWRSLLFGRELLAKGVRWGVGDGKTIKIFSDNWIPGFRPQLVTTLSPFPTDATVS 976

Query: 199  NLFKTNSREWDEDIIKELCNDRDRMSIYDIVLCRNQYIDTRYWYYEQNGNYTVKSAYKLL 378
             L   ++R WD D+I+ L        I  I + R+   D   W +++ G Y+V+SAY L 
Sbjct: 977  CLMNEDARCWDGDLIRSLFPVDIAKEILQIPISRHGDADFASWPHDKLGLYSVRSAYNLA 1036

Query: 379  QS--FYGRWEEN---------DEVKLWRSLWKLQVPAKVKVFLWRALNNCLPTKCNLQTR 525
            +S  F+     +         +  K W+ LWK+  P K+K+ LWRA + CL T   L+ R
Sbjct: 1037 RSEAFFADQSNSGRGMASRLLESQKDWKGLWKINAPGKMKITLWRAAHECLATGFQLRRR 1096

Query: 526  MVQTDDVCPVCGMD 567
             + + D C  C  D
Sbjct: 1097 HIPSTDGCVFCNRD 1110



 Score = 48.9 bits (115), Expect(2) = 2e-31
 Identities = 35/147 (23%), Positives = 54/147 (36%), Gaps = 5/147 (3%)
 Frame = +2

Query: 569  RRQHASHCFIHCLFAKSCWHIV----CPESNFNSTMNFSMWFLYMLEHHKEKA-AAISMV 733
            R     H F+ C FA   W  +      +   N       W    L+     A   +++ 
Sbjct: 1109 RDDTVEHVFLFCPFAAQIWEEIKGKCAVKLGRNGFSTMRQWIFDFLKRGSSHANTLLAVT 1168

Query: 734  CWSIWRARNDVVWNNTNWRAGNVVTSAVAYLTQWNDVQNLGKATSPTPAHSTGDTEQWNK 913
             W IW ARN+   NN       VV   ++Y+     +++  K        +T    +W  
Sbjct: 1169 FWHIWEARNNTKNNNGTVHPQRVVIKILSYVDM--ILKHNTKTVDGQRGGNTQAIPRWQP 1226

Query: 914  PEIGCVKVNCDATIFKSSSQFSVGWIV 994
            P      +N DA IF SS    VG ++
Sbjct: 1227 PPASVWMINSDAAIFSSSRTMGVGALI 1253