BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00015220
         (1185 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ABD33126.2| RNA-directed DNA polymerase (Reverse transcriptas...   246   1e-65
gb|ABN08038.1| RNA-directed DNA polymerase (Reverse transcriptas...   221   4e-61
emb|CCA66054.1| hypothetical protein [Beta vulgaris subsp. vulga...   214   7e-58
emb|CCA66044.1| hypothetical protein [Beta vulgaris subsp. vulga...   207   9e-56
gb|AAG51783.1|AC079679_3 reverse transcriptase, putative; 16838-...   208   1e-55

>gb|ABD33126.2| RNA-directed DNA polymerase (Reverse transcriptase) [Medicago
            truncatula]
          Length = 653

 Score =  246 bits (629), Expect(3) = 1e-65
 Identities = 108/247 (43%), Positives = 169/247 (68%)
 Frame = -2

Query: 1019 KANQEEATIVKNLLVNYERLSGQSINFQKSGVFYSSNVRRDKQHEISSILGVFNDISNNN 840
            +AN+ E   +K++L  Y   S Q IN QKS +++S NV   K++ +S++L V   I    
Sbjct: 282  RANENETRALKDILDTYANASDQLINMQKSEIYFSRNVPVTKKNTLSNMLWVSEGIGIRK 341

Query: 839  YLGLPSLVGRSKKRVFSFLKDKISKRIQGWRSKPISRAGKSVLIRNVARSIPSYCMSCFL 660
            YLGLPS++GRSKK +F+++KD+I  RI G  SK +S+AGK VLI++VA++IPSYCMS FL
Sbjct: 342  YLGLPSMIGRSKKSIFNYIKDRIWNRISGLSSKMLSQAGKEVLIKSVAQAIPSYCMSVFL 401

Query: 659  LPKMLCQELERSFNNFWWKSSTREGKGINWLNWDGMSMSKSRGGLGFRNLYGFNIALLGK 480
            LP  +  ++E+  N+FWW        GI W++W+ ++M K  GG+GFR+++GF++A+ GK
Sbjct: 402  LPHSIADDIEKMLNSFWWGMKNEGRGGIRWMSWERLTMRKEWGGMGFRHIHGFDLAMSGK 461

Query: 479  NCWNLMTNSSSLVARVYKARYYTDTHFLKSRKGQGVSFIWAGIWSAKEVLSKSFRWVLGD 300
             CWN +TN  ++V+R++KA+Y+T+ +FL +  G   S++W  IWS++  L   ++W +GD
Sbjct: 462  QCWNFLTNPDAMVSRIFKAKYFTNENFLGASLGHNPSYVWRSIWSSRIFLKTGYQWRIGD 521

Query: 299  GSDIVAV 279
            GS    V
Sbjct: 522  GSTTATV 528



 Score = 29.3 bits (64), Expect(3) = 1e-65
 Identities = 14/60 (23%), Positives = 26/60 (43%)
 Frame = -1

Query: 204 VSSFFIPNSKQWDTCRVYESFLEEDANAILAVHIPQRDVKDKVAWVRSNDGKYNVKTGYQ 25
           VS      ++ W+   +  +F   D + IL + +   +  D + W     G Y+VK  Y+
Sbjct: 528 VSHLIDAQTRSWNEPLIRNNFNLRDVDEILKIPLLHCEQNDAIIWRFDKKGVYSVKNAYR 587



 Score = 22.7 bits (47), Expect(3) = 1e-65
 Identities = 12/43 (27%), Positives = 21/43 (48%)
 Frame = -1

Query: 1185 GNTIIHQITILCY*YTKRSIDRAAMSGMLNGCCISSSAPVVTH 1057
            G+ +   + ILC       I +A  + +L+G  +   AP V+H
Sbjct: 228  GDPLSPYLFILCAEGMSTLIKQAERNNILHGVKVCKRAPTVSH 270


>gb|ABN08038.1| RNA-directed DNA polymerase (Reverse transcriptase) [Medicago
           truncatula]
          Length = 573

 Score =  221 bits (563), Expect(2) = 4e-61
 Identities = 100/205 (48%), Positives = 142/205 (69%)
 Frame = -2

Query: 902 RDKQHEISSILGVFNDISNNNYLGLPSLVGRSKKRVFSFLKDKISKRIQGWRSKPISRAG 723
           +D    I++ LGV N+I +  YLGLPS+VGR+K  +FS+LKD+I K+ Q W  + +SRA 
Sbjct: 260 KDIISSITASLGVHNNIGSGKYLGLPSMVGRNKNAIFSYLKDRIWKKCQSWSVRSLSRAD 319

Query: 722 KSVLIRNVARSIPSYCMSCFLLPKMLCQELERSFNNFWWKSSTREGKGINWLNWDGMSMS 543
           K VLI++VA++IPSYCM  FL+P  LC+ELER  N+F+W S     +GINW+ WD +++ 
Sbjct: 320 KEVLIKSVAQAIPSYCMGAFLIPASLCEELERMMNSFYWGSKKNGRRGINWMRWDFLTLH 379

Query: 542 KSRGGLGFRNLYGFNIALLGKNCWNLMTNSSSLVARVYKARYYTDTHFLKSRKGQGVSFI 363
           KS GGLGFRNL  FN+++LGK  W L+++SSSL  R+ KA+Y+    FL +  G   S+ 
Sbjct: 380 KSLGGLGFRNLEAFNLSMLGKQGWKLLSDSSSLFTRILKAKYFPRRDFLDATLGHNPSYT 439

Query: 362 WAGIWSAKEVLSKSFRWVLGDGSDI 288
           W  +WS + +L+   RW +GDGS I
Sbjct: 440 WRSLWSTQSLLTLGHRWKIGDGSKI 464



 Score = 41.2 bits (95), Expect(2) = 4e-61
 Identities = 28/88 (31%), Positives = 42/88 (47%)
 Frame = -1

Query: 276 RSMLRLKDDFLVTSNSIYEGRDERVSSFFIPNSKQWDTCRVYESFLEEDANAILAVHIPQ 97
           R++L LK     TS  +++  D  V+S    +   WDT  V   F + +A AIL+  +  
Sbjct: 473 RNLLSLKPS---TSPPLHQ-HDLTVNSLLSADFNSWDTTLVQSLFNDAEAAAILSTPLFA 528

Query: 96  RDVKDKVAWVRSNDGKYNVKTGYQFWHD 13
           R   D   W  + DG Y VK+ Y+   D
Sbjct: 529 RSGSDVRIWKATVDGTYTVKSAYRICSD 556


>emb|CCA66054.1| hypothetical protein [Beta vulgaris subsp. vulgaris]
          Length = 1355

 Score =  214 bits (546), Expect(2) = 7e-58
 Identities = 105/250 (42%), Positives = 158/250 (63%)
 Frame = -2

Query: 1019 KANQEEATIVKNLLVNYERLSGQSINFQKSGVFYSSNVRRDKQHEISSILGVFNDISNNN 840
            +AN++E TI+ ++L  YE  SGQ IN++KS V YS  V   ++ E+++IL +     +  
Sbjct: 694  RANRQECTIIVDILNQYELASGQKINYEKSEVSYSRGVSVSQKDELTNILNMRQVDRHEK 753

Query: 839  YLGLPSLVGRSKKRVFSFLKDKISKRIQGWRSKPISRAGKSVLIRNVARSIPSYCMSCFL 660
            YLG+PS+ GRSKK +F  L D+I K++QGW+ K +SRAGK VL+++V ++IP+Y M  + 
Sbjct: 754  YLGIPSISGRSKKAIFDSLIDRIWKKLQGWKEKLLSRAGKEVLLKSVIQAIPTYLMGVYK 813

Query: 659  LPKMLCQELERSFNNFWWKSSTREGKGINWLNWDGMSMSKSRGGLGFRNLYGFNIALLGK 480
             P  + Q+++ +   FWW SS  + K I+W NWD M   K  GG+GF++L  FN ALLG+
Sbjct: 814  FPVFIIQKIQSAMARFWWGSSDTQRK-IHWKNWDSMCNLKCFGGMGFKDLTIFNDALLGR 872

Query: 479  NCWNLMTNSSSLVARVYKARYYTDTHFLKSRKGQGVSFIWAGIWSAKEVLSKSFRWVLGD 300
              W L     SL+ RV KA+Y+ +  FL +  G   S+ W+ IWS+K +L +   W +G+
Sbjct: 873  QAWRLTREPQSLLGRVMKAKYFPNCDFLNAPLGHSSSYSWSSIWSSKALLKEGVIWRVGN 932

Query: 299  GSDIVAVKDP 270
            GS I    DP
Sbjct: 933  GSQINMWSDP 942



 Score = 37.0 bits (84), Expect(2) = 7e-58
 Identities = 21/58 (36%), Positives = 29/58 (50%), Gaps = 1/58 (1%)
 Frame = -1

Query: 174  QWDTCRVYESFLEE-DANAILAVHIPQRDVKDKVAWVRSNDGKYNVKTGYQFWHDNNV 4
            +W T  + ESFL E D   ILA  +    V D++ W  + D  Y+VKT Y      N+
Sbjct: 973  EWKTS-LLESFLNERDLRCILASPLSATPVPDELTWAFTKDATYSVKTAYMIGKGGNL 1029


>emb|CCA66044.1| hypothetical protein [Beta vulgaris subsp. vulgaris]
          Length = 1355

 Score =  207 bits (528), Expect(2) = 9e-56
 Identities = 100/250 (40%), Positives = 159/250 (63%)
 Frame = -2

Query: 1019 KANQEEATIVKNLLVNYERLSGQSINFQKSGVFYSSNVRRDKQHEISSILGVFNDISNNN 840
            +A+++E  I+  +L  YE+ SGQ IN+ KS V +S  V   ++ E+S+IL +     +  
Sbjct: 694  RASRQECAIIVEILNLYEQASGQKINYDKSEVSFSKGVSIAQKEELSNILQMKQVERHMK 753

Query: 839  YLGLPSLVGRSKKRVFSFLKDKISKRIQGWRSKPISRAGKSVLIRNVARSIPSYCMSCFL 660
            YLG+PS+ GRS+  +F  L D+I K++QGW+ K +SRAGK +L+++V ++IP+Y M  + 
Sbjct: 754  YLGIPSITGRSRTAIFDSLMDRIWKKLQGWKEKLLSRAGKEILLKSVIQAIPTYLMGVYK 813

Query: 659  LPKMLCQELERSFNNFWWKSSTREGKGINWLNWDGMSMSKSRGGLGFRNLYGFNIALLGK 480
            LP  + Q++  +   FWW SS  + + I+W NWD +   K  GG+GFR+L  FN ALLG+
Sbjct: 814  LPCSIIQKIHSAMARFWWGSSDTQ-RRIHWKNWDSLCTLKCFGGMGFRDLRVFNDALLGR 872

Query: 479  NCWNLMTNSSSLVARVYKARYYTDTHFLKSRKGQGVSFIWAGIWSAKEVLSKSFRWVLGD 300
              W L+    SL+ARV KA+YY++  FL +  G   S+ W  IWS+K +L +   W +G+
Sbjct: 873  QAWRLVREPHSLLARVMKAKYYSNHDFLDAPLGVSTSYSWRSIWSSKALLKEGMVWRIGN 932

Query: 299  GSDIVAVKDP 270
            G+++   +DP
Sbjct: 933  GTNVRIWEDP 942



 Score = 37.0 bits (84), Expect(2) = 9e-56
 Identities = 18/72 (25%), Positives = 31/72 (43%)
 Frame = -1

Query: 219  GRDERVSSFFIPNSKQWDTCRVYESFLEEDANAILAVHIPQRDVKDKVAWVRSNDGKYNV 40
            G    VS     +  +W    +   F E D   IL++ +    +KD++ W  + +  Y+V
Sbjct: 958  GNLNMVSELIDFDRMEWKVSLIETVFNERDIKCILSIPLSSLPLKDELTWAFTKNAHYSV 1017

Query: 39   KTGYQFWHDNNV 4
            KT Y      N+
Sbjct: 1018 KTAYMLGKGGNL 1029


>gb|AAG51783.1|AC079679_3 reverse transcriptase, putative; 16838-20266 [Arabidopsis thaliana]
          Length = 1142

 Score =  208 bits (529), Expect(3) = 1e-55
 Identities = 105/250 (42%), Positives = 153/250 (61%)
 Frame = -2

Query: 1019 KANQEEATIVKNLLVNYERLSGQSINFQKSGVFYSSNVRRDKQHEISSILGVFNDISNNN 840
            KAN+E+  I+  +L  YE +SGQ INF KS + +   V    + +I  ILG+ N     +
Sbjct: 474  KANKEQCGIILEILKQYESVSGQQINFSKSSIQFGHKVEDSIKADIKLILGIHNLGGMGS 533

Query: 839  YLGLPSLVGRSKKRVFSFLKDKISKRIQGWRSKPISRAGKSVLIRNVARSIPSYCMSCFL 660
            YLGLP  +G SK +VFSF++D++  RI GW +K +S+ GK V+I++VA ++P Y MSCF 
Sbjct: 534  YLGLPESLGGSKTKVFSFVRDRLQSRINGWSAKFLSKGGKEVMIKSVAATLPRYVMSCFR 593

Query: 659  LPKMLCQELERSFNNFWWKSSTREGKGINWLNWDGMSMSKSRGGLGFRNLYGFNIALLGK 480
            LPK +  +L  +   FWW SS  + +G++W+ WD +  SKS GGLGFRN+  FN ALL K
Sbjct: 594  LPKAITSKLTSAVAKFWW-SSNGDSRGMHWMAWDKLCSSKSDGGLGFRNVDDFNSALLAK 652

Query: 479  NCWNLMTNSSSLVARVYKARYYTDTHFLKSRKGQGVSFIWAGIWSAKEVLSKSFRWVLGD 300
              W L+T   SL A+V+K RY+  ++ L S K    S+ W  + SA+ ++ K     +G 
Sbjct: 653  QLWRLITAPDSLFAKVFKGRYFRKSNPLDSIKSYSPSYGWRSMISARSLVYKGLIKRVGS 712

Query: 299  GSDIVAVKDP 270
            G+ I    DP
Sbjct: 713  GASISVWNDP 722



 Score = 34.7 bits (78), Expect(3) = 1e-55
 Identities = 19/60 (31%), Positives = 29/60 (48%)
 Frame = -1

Query: 207 RVSSFFIPNSKQWDTCRVYESFLEEDANAILAVHIPQRDVKDKVAWVRSNDGKYNVKTGY 28
           +V S     S  W+   + E F  ED   I A+ I   +++D + W  +  G Y VK+GY
Sbjct: 744 KVKSLIDSRSNFWNIDLLKELFDPEDVPLISALPIGNPNMEDTLGWHFTKAGNYTVKSGY 803



 Score = 22.3 bits (46), Expect(3) = 1e-55
 Identities = 10/43 (23%), Positives = 22/43 (51%)
 Frame = -1

Query: 1185 GNTIIHQITILCY*YTKRSIDRAAMSGMLNGCCISSSAPVVTH 1057
            G+ +   + ILC      +I +A    ++ G  +++ +P V+H
Sbjct: 420  GDPLSPYLFILCTEVLIANIRKAERQNLITGIKVATPSPAVSH 462