BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00015109
         (974 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002273627.2| PREDICTED: diphthamide biosynthesis protein ...   441   e-121
ref|XP_002326199.1| predicted protein [Populus trichocarpa] gi|2...   397   e-108
ref|XP_002530564.1| diphteria toxin resistance protein 2, dph2, ...   396   e-108
ref|XP_003546007.1| PREDICTED: diphthamide biosynthesis protein ...   383   e-104
ref|XP_004152127.1| PREDICTED: diphthamide biosynthesis protein ...   378   e-102

>ref|XP_002273627.2| PREDICTED: diphthamide biosynthesis protein 2-like [Vitis vinifera]
            gi|296084273|emb|CBI24661.3| unnamed protein product
            [Vitis vinifera]
          Length = 506

 Score =  441 bits (1133), Expect = e-121
 Identities = 219/314 (69%), Positives = 257/314 (81%)
 Frame = -2

Query: 973  FGKALISASDTAESLCEFALASVKPILVLFGLEYAYALQEIKEMAIVESLKLCGLASKLD 794
            FGKA IS S+ AE+L  +AL   K I+VLFGLEY++A++ IKE  ++ES KL G  S L+
Sbjct: 109  FGKAPISISNCAENLSTYALTQGKHIMVLFGLEYSHAIEMIKEALMLESSKLSGSTSNLE 168

Query: 793  ILYAEVICSIMNPLKKFETSNEHLDSVSESTSDGCTGKTTGLSYKIGGLGWNIPEGCSID 614
              +AEV+CS+MNP K   TSN HL SV   T++   GK TG  Y IGGL WN+PEG  ++
Sbjct: 169  FHFAEVMCSVMNPSKDCTTSNGHLGSVGSFTANEGFGKATGTRYSIGGLAWNLPEGHRME 228

Query: 613  DYTLFWIGSDNSAFANVVLTFNGCEIVRYDATENHIITDFSQQRRVLKRRYYLVEKAKDA 434
            DY L WIG+DNSAFANVVLTFNGC+IVRYDA +N ++TD SQQRR+LKRRYYLVEKAKD+
Sbjct: 229  DYLLVWIGADNSAFANVVLTFNGCDIVRYDAVDNRLVTDLSQQRRILKRRYYLVEKAKDS 288

Query: 433  SIVGILVGTLGVAGYLCMIHQMKELITKAGKKVYILAMGRPNPAKLANFPECDVFIYVSC 254
            +I+G+LVGTLGVAGYL MI QMKELI  AGKK Y L MGRPNPAKLANFPECDVFIYVSC
Sbjct: 289  NIIGLLVGTLGVAGYLHMIRQMKELIAAAGKKAYTLVMGRPNPAKLANFPECDVFIYVSC 348

Query: 253  AQTALLDSKEFLAPVITPFEAMLAFSSGTDWTGAYVMEFQQLISSTPVEGRSLSKEARFS 74
            AQTALLDSKEFLAPVITPFEAMLAF+ G+ WTGAYVMEF+ LISS+PVE R+ S+EARFS
Sbjct: 349  AQTALLDSKEFLAPVITPFEAMLAFNRGSQWTGAYVMEFRDLISSSPVEVRNQSEEARFS 408

Query: 73   FIHGGYVEDHNLQE 32
            F+ GGYVED +L+E
Sbjct: 409  FLQGGYVEDFDLKE 422


>ref|XP_002326199.1| predicted protein [Populus trichocarpa] gi|222833392|gb|EEE71869.1|
            predicted protein [Populus trichocarpa]
          Length = 496

 Score =  397 bits (1020), Expect = e-108
 Identities = 204/314 (64%), Positives = 238/314 (75%)
 Frame = -2

Query: 973  FGKALISASDTAESLCEFALASVKPILVLFGLEYAYALQEIKEMAIVESLKLCGLASKLD 794
            FGKA+I  S  A SL  +A  + KP LVLFGLEYA+A+  I+E  +  +  L G  +K  
Sbjct: 104  FGKAMIDVSSCAASLSNYASNNSKPFLVLFGLEYAHAIPYIREELVAAASTLSGSKTKTV 163

Query: 793  ILYAEVICSIMNPLKKFETSNEHLDSVSESTSDGCTGKTTGLSYKIGGLGWNIPEGCSID 614
              +AEV+C ++NP    + S     SV +       G      + IGGL W +P G  ++
Sbjct: 164  FHFAEVMCPLINPPDCHKDSGGLPRSVGDGEVSNELGVAACSRHTIGGLSWELPSGQKME 223

Query: 613  DYTLFWIGSDNSAFANVVLTFNGCEIVRYDATENHIITDFSQQRRVLKRRYYLVEKAKDA 434
            DY L WIGSDNSAFANVVLTFNGCEIVRYDA  N + TD SQQRR+LKRRYYLVEKAKDA
Sbjct: 224  DYLLLWIGSDNSAFANVVLTFNGCEIVRYDANGNFLGTDLSQQRRILKRRYYLVEKAKDA 283

Query: 433  SIVGILVGTLGVAGYLCMIHQMKELITKAGKKVYILAMGRPNPAKLANFPECDVFIYVSC 254
            +IVGILVGTLGVAGYL MIHQMK+LIT AGKKVY L MGRPNPAKLANFPECDVFIYVSC
Sbjct: 284  NIVGILVGTLGVAGYLNMIHQMKDLITGAGKKVYTLVMGRPNPAKLANFPECDVFIYVSC 343

Query: 253  AQTALLDSKEFLAPVITPFEAMLAFSSGTDWTGAYVMEFQQLISSTPVEGRSLSKEARFS 74
            AQTALLDSK+F APVITPFEAMLAF+ G++WTGAYVMEF+ LI+S+PVE +S S+EARFS
Sbjct: 344  AQTALLDSKDFYAPVITPFEAMLAFNRGSEWTGAYVMEFRDLITSSPVEVKSYSEEARFS 403

Query: 73   FIHGGYVEDHNLQE 32
            F+ G YVED +LQE
Sbjct: 404  FVQGRYVEDFDLQE 417


>ref|XP_002530564.1| diphteria toxin resistance protein 2, dph2, putative [Ricinus
            communis] gi|223529863|gb|EEF31794.1| diphteria toxin
            resistance protein 2, dph2, putative [Ricinus communis]
          Length = 499

 Score =  396 bits (1017), Expect = e-108
 Identities = 197/314 (62%), Positives = 242/314 (77%)
 Frame = -2

Query: 973  FGKALISASDTAESLCEFALASVKPILVLFGLEYAYALQEIKEMAIVESLKLCGLASKLD 794
            FGKA I+ S  A+SL ++A +S KPI+VL+GLEYA+A+  I++  ++ +  +       +
Sbjct: 108  FGKASINVSSCAQSLSDYAFSSTKPIMVLYGLEYAHAIPHIRQ-ELIAAASMQSRPRHSE 166

Query: 793  ILYAEVICSIMNPLKKFETSNEHLDSVSESTSDGCTGKTTGLSYKIGGLGWNIPEGCSID 614
            + +A+V+CS +NP    + S+E    + +  +    G+  G  +KIGGL W++  G  ++
Sbjct: 167  LHFADVMCSAINPSNNQKKSDEPARLIGDCVATNDVGEAAGCRHKIGGLIWDLSRGQRME 226

Query: 613  DYTLFWIGSDNSAFANVVLTFNGCEIVRYDATENHIITDFSQQRRVLKRRYYLVEKAKDA 434
            DY L WIGS NSAFANVVLTFNGCEIVRYDA E+ ++TD SQQRR+LKRRYYLVEKAKDA
Sbjct: 227  DYLLVWIGSGNSAFANVVLTFNGCEIVRYDAKESRLVTDLSQQRRILKRRYYLVEKAKDA 286

Query: 433  SIVGILVGTLGVAGYLCMIHQMKELITKAGKKVYILAMGRPNPAKLANFPECDVFIYVSC 254
            +IVGILVGTLGVAGYL MIHQMK+LIT AGKK Y L MGRPNPAKLANFPECD+FIYVSC
Sbjct: 287  NIVGILVGTLGVAGYLDMIHQMKDLITSAGKKAYTLVMGRPNPAKLANFPECDIFIYVSC 346

Query: 253  AQTALLDSKEFLAPVITPFEAMLAFSSGTDWTGAYVMEFQQLISSTPVEGRSLSKEARFS 74
            AQTALLDSKEFLAPVITPFEAM+AF+ G+ WTGAYVMEF+ LI S+PVE      EARFS
Sbjct: 347  AQTALLDSKEFLAPVITPFEAMIAFNRGSQWTGAYVMEFRDLIDSSPVEAMIKPAEARFS 406

Query: 73   FIHGGYVEDHNLQE 32
            FI G YVED +LQE
Sbjct: 407  FIQGRYVEDFDLQE 420


>ref|XP_003546007.1| PREDICTED: diphthamide biosynthesis protein 2-like [Glycine max]
          Length = 485

 Score =  383 bits (984), Expect = e-104
 Identities = 199/315 (63%), Positives = 240/315 (76%), Gaps = 1/315 (0%)
 Frame = -2

Query: 973  FGKALISASDTAESLCEFALASVKPILVLFGLEYAYALQEIKEMAIVESLKLCGLASKLD 794
            FGKA I  +D  ES+ ++AL + KPI+VLFGLEYA+++Q IKE A++ES   C    K +
Sbjct: 100  FGKASICIADCVESMSKYALTNSKPIMVLFGLEYAHSMQRIKE-ALLESSMSCRFDPKSE 158

Query: 793  ILYAEVICSIMNPLKKFETSNEHLDSVSESTSDGCTGKTTGLSYKIGGLGWNIPEGCSID 614
            + +A+V  S+M P K  +  N   + VS+     C G  +G  Y IGGL W +PEG S+D
Sbjct: 159  VHFADVPSSVMFPSKDIKKINVLQEPVSD-----CNG-ASGTMYSIGGLTWKLPEGQSMD 212

Query: 613  DYTLFWIGSDNSAFANVVLTFNGCEIVRYDATENHIITDFSQQRRVLKRRYYLVEKAKDA 434
            DY +FWIG D+SAFANVVLTFN CEIVRYDA EN ++TD  Q RR+LKRRYYLVE+AKDA
Sbjct: 213  DYLVFWIGHDDSAFANVVLTFNACEIVRYDANENRMVTDLFQHRRILKRRYYLVERAKDA 272

Query: 433  SIVGILVGTLGVAGYLCMIHQMKELITKAGKKVYILAMGRPNPAKLANFPECDVFIYVSC 254
            +IVGILVGTLGVAGYL +I+QM ELIT AGKK Y L MGRPNPAKLANFPECDVF+YVSC
Sbjct: 273  NIVGILVGTLGVAGYLHIINQMMELITGAGKKAYTLVMGRPNPAKLANFPECDVFLYVSC 332

Query: 253  AQTALLDSKEFLAPVITPFEAMLAFSSGTDWTGAYVMEFQQLISSTPV-EGRSLSKEARF 77
            AQTALLDSKE+LAPVITPFEAM+AF+ G+ WTGAYVMEF+ LI+   +  G    +EARF
Sbjct: 333  AQTALLDSKEYLAPVITPFEAMIAFNRGSQWTGAYVMEFRDLINLPQMGVGNQEEEEARF 392

Query: 76   SFIHGGYVEDHNLQE 32
            SF+ GGYVED   QE
Sbjct: 393  SFLKGGYVEDFENQE 407


>ref|XP_004152127.1| PREDICTED: diphthamide biosynthesis protein 2-like [Cucumis sativus]
            gi|449484704|ref|XP_004156957.1| PREDICTED: diphthamide
            biosynthesis protein 2-like [Cucumis sativus]
          Length = 500

 Score =  378 bits (970), Expect = e-102
 Identities = 193/323 (59%), Positives = 245/323 (75%)
 Frame = -2

Query: 973  FGKALISASDTAESLCEFALASVKPILVLFGLEYAYALQEIKEMAIVESLKLCGLASKLD 794
            FGK  I     A++L +++L+S KP+LVLFGLEYA+++ +IK+  +++S +   L S+L+
Sbjct: 112  FGKDSIDVPTCAKTLSQYSLSSGKPVLVLFGLEYAHSMLDIKQR-LLDSFQTSSLGSELE 170

Query: 793  ILYAEVICSIMNPLKKFETSNEHLDSVSESTSDGCTGKTTGLSYKIGGLGWNIPEGCSID 614
            + +A+V CS ++P    E   +  +  S + S G +    G  + IGGL W +P+   + 
Sbjct: 171  VHFADVKCSSLDPSPHHENVVKGGEQASNAES-GSSESIAGARHHIGGLFWELPKERRVK 229

Query: 613  DYTLFWIGSDNSAFANVVLTFNGCEIVRYDATENHIITDFSQQRRVLKRRYYLVEKAKDA 434
            D +LFWIGS+NSAFANVVLTFNGCEIVRYDA E+ ++TD S+QRR+LKRRYYLVEKAKDA
Sbjct: 230  DCSLFWIGSENSAFANVVLTFNGCEIVRYDAKESWLVTDVSKQRRILKRRYYLVEKAKDA 289

Query: 433  SIVGILVGTLGVAGYLCMIHQMKELITKAGKKVYILAMGRPNPAKLANFPECDVFIYVSC 254
            +IVGILVGTLG+AGYL +IHQMKELIT AGKK Y L MG+PNPAKLANFPEC VFIYVSC
Sbjct: 290  AIVGILVGTLGLAGYLHIIHQMKELITGAGKKAYTLVMGKPNPAKLANFPECGVFIYVSC 349

Query: 253  AQTALLDSKEFLAPVITPFEAMLAFSSGTDWTGAYVMEFQQLISSTPVEGRSLSKEARFS 74
            AQTAL+DSKE+LAPVITPFEA LAFS G+ WTGAYVMEFQ LI  +  E  + S EAR+S
Sbjct: 350  AQTALMDSKEYLAPVITPFEATLAFSRGSQWTGAYVMEFQDLIDFSTPEEANRSDEARYS 409

Query: 73   FIHGGYVEDHNLQEVYQKRQGFH 5
            F+ GGYVED + QE  ++  G H
Sbjct: 410  FLQGGYVEDWDSQENTEEENGAH 432