BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00015090
         (529 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003638044.1| 4-coumarate-coa ligase [Medicago truncatula]...   310   8e-83
gb|AFD33347.1| acyl-activating enzyme 3 [Cannabis sativa]             307   7e-82
ref|XP_002329323.1| 4-coumarate-coa ligase [Populus trichocarpa]...   307   7e-82
emb|CBI17160.3| unnamed protein product [Vitis vinifera]              306   1e-81
ref|XP_002305282.1| 4-coumarate-coa ligase [Populus trichocarpa]...   306   1e-81

>ref|XP_003638044.1| 4-coumarate-coa ligase [Medicago truncatula]
           gi|355503979|gb|AES85182.1| 4-coumarate-coa ligase
           [Medicago truncatula]
          Length = 218

 Score =  310 bits (794), Expect = 8e-83
 Identities = 149/174 (85%), Positives = 164/174 (94%)
 Frame = -3

Query: 524 RHTGSAGMLVPGVESQIVDVTTLKSLPPNQLGEIWVRGPNMMKGYFNNPQATELTIDTQG 345
           RHTGSAGMLV GVE+QIV V TLK LPP QLGEIWVRGPNMM GY+NNPQA+ LTID +G
Sbjct: 33  RHTGSAGMLVAGVEAQIVSVDTLKPLPPGQLGEIWVRGPNMMPGYYNNPQASRLTIDKKG 92

Query: 344 WVHTGDLGYFDDEGQLFVVDRIKELIKYKGFQVAPAELEGLLVSHSEILDAVVIPFPDAE 165
           WVHTGDLGYFD++G LFVVDRIKELIKYKGFQVAPAELEGLLVSH EILDAVVIPFPDAE
Sbjct: 93  WVHTGDLGYFDEDGNLFVVDRIKELIKYKGFQVAPAELEGLLVSHPEILDAVVIPFPDAE 152

Query: 164 AGEVPIAYVVRSPNSSLTEEDVKKFIAEQVAPFKRLRKITFVNSVPKSASGKIL 3
           AGEVP+AYVVRSPNSSLTEED++KFIA+QVAPFKRLR++TF+N+VPK+ASGKIL
Sbjct: 153 AGEVPVAYVVRSPNSSLTEEDIQKFIADQVAPFKRLRRVTFINTVPKTASGKIL 206


>gb|AFD33347.1| acyl-activating enzyme 3 [Cannabis sativa]
          Length = 543

 Score =  307 bits (786), Expect = 7e-82
 Identities = 150/174 (86%), Positives = 163/174 (93%)
 Frame = -3

Query: 524 RHTGSAGMLVPGVESQIVDVTTLKSLPPNQLGEIWVRGPNMMKGYFNNPQATELTIDTQG 345
           R++GSAGML  GVE+QIV V TLK LPPNQLGEIWV+GPNMM+GYFNNPQAT+LTID +G
Sbjct: 358 RNSGSAGMLASGVEAQIVSVDTLKPLPPNQLGEIWVKGPNMMQGYFNNPQATKLTIDKKG 417

Query: 344 WVHTGDLGYFDDEGQLFVVDRIKELIKYKGFQVAPAELEGLLVSHSEILDAVVIPFPDAE 165
           WVHTGDLGYFD++G L+VVDRIKELIKYKGFQVAPAELEGLLVSH EILDAVVIPFPDAE
Sbjct: 418 WVHTGDLGYFDEDGHLYVVDRIKELIKYKGFQVAPAELEGLLVSHPEILDAVVIPFPDAE 477

Query: 164 AGEVPIAYVVRSPNSSLTEEDVKKFIAEQVAPFKRLRKITFVNSVPKSASGKIL 3
           AGEVP+AYVVRSPNSSLTE DVKKFIA QVA FKRLRK+TF+NSVPKSASGKIL
Sbjct: 478 AGEVPVAYVVRSPNSSLTENDVKKFIAGQVASFKRLRKVTFINSVPKSASGKIL 531


>ref|XP_002329323.1| 4-coumarate-coa ligase [Populus trichocarpa]
           gi|224127626|ref|XP_002329324.1| acyl:coa ligase
           [Populus trichocarpa] gi|222870777|gb|EEF07908.1|
           4-coumarate-coa ligase [Populus trichocarpa]
           gi|222870778|gb|EEF07909.1| acyl:coa ligase [Populus
           trichocarpa]
          Length = 543

 Score =  307 bits (786), Expect = 7e-82
 Identities = 147/174 (84%), Positives = 164/174 (94%)
 Frame = -3

Query: 524 RHTGSAGMLVPGVESQIVDVTTLKSLPPNQLGEIWVRGPNMMKGYFNNPQATELTIDTQG 345
           RH+GSAG+L  G+E+QI+ V T K LPPNQLGEIWVRGPNMM+GYFNNPQAT+ TID +G
Sbjct: 358 RHSGSAGILNAGIEAQIISVETAKPLPPNQLGEIWVRGPNMMRGYFNNPQATKDTIDKKG 417

Query: 344 WVHTGDLGYFDDEGQLFVVDRIKELIKYKGFQVAPAELEGLLVSHSEILDAVVIPFPDAE 165
           WVHTGDLGYFDD+GQLFVVDRIKELIKYKGFQVAPAELEGLLVSH EILDAVV P+PDAE
Sbjct: 418 WVHTGDLGYFDDDGQLFVVDRIKELIKYKGFQVAPAELEGLLVSHPEILDAVVTPYPDAE 477

Query: 164 AGEVPIAYVVRSPNSSLTEEDVKKFIAEQVAPFKRLRKITFVNSVPKSASGKIL 3
           AGEVP+AYVVRSPNS+LTEEDV+KFI++QVAPFKRLRK+TF+NSVPKSASGKIL
Sbjct: 478 AGEVPVAYVVRSPNSALTEEDVQKFISDQVAPFKRLRKVTFINSVPKSASGKIL 531


>emb|CBI17160.3| unnamed protein product [Vitis vinifera]
          Length = 221

 Score =  306 bits (784), Expect = 1e-81
 Identities = 148/174 (85%), Positives = 163/174 (93%)
 Frame = -3

Query: 524 RHTGSAGMLVPGVESQIVDVTTLKSLPPNQLGEIWVRGPNMMKGYFNNPQATELTIDTQG 345
           +HTGS G LV GVE+QI+ V TLK LPPNQLGEIWVRGPNMMKGYFNNPQAT+LTID +G
Sbjct: 36  QHTGSTGPLVSGVEAQIISVDTLKPLPPNQLGEIWVRGPNMMKGYFNNPQATKLTIDKKG 95

Query: 344 WVHTGDLGYFDDEGQLFVVDRIKELIKYKGFQVAPAELEGLLVSHSEILDAVVIPFPDAE 165
           WVHTGD+GYFD++G+LFVVDRIKELIKYKGFQVAPAELE LLVSH EILDAVVIPFPDAE
Sbjct: 96  WVHTGDVGYFDEQGKLFVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPFPDAE 155

Query: 164 AGEVPIAYVVRSPNSSLTEEDVKKFIAEQVAPFKRLRKITFVNSVPKSASGKIL 3
           AGEVPIAYVVRSPNSSLTEEDVK FIA QVAPFK+LR+++F+N+VPKSASGKIL
Sbjct: 156 AGEVPIAYVVRSPNSSLTEEDVKTFIANQVAPFKKLRRVSFINTVPKSASGKIL 209


>ref|XP_002305282.1| 4-coumarate-coa ligase [Populus trichocarpa]
           gi|222848246|gb|EEE85793.1| 4-coumarate-coa ligase
           [Populus trichocarpa]
          Length = 543

 Score =  306 bits (784), Expect = 1e-81
 Identities = 146/174 (83%), Positives = 165/174 (94%)
 Frame = -3

Query: 524 RHTGSAGMLVPGVESQIVDVTTLKSLPPNQLGEIWVRGPNMMKGYFNNPQATELTIDTQG 345
           RH+GSAG+L  G+E+QIV V TLK LPPNQLGEIWVRGPNMM+GYFNNPQAT+ T+D +G
Sbjct: 358 RHSGSAGILAAGIEAQIVSVETLKPLPPNQLGEIWVRGPNMMRGYFNNPQATKDTMDKKG 417

Query: 344 WVHTGDLGYFDDEGQLFVVDRIKELIKYKGFQVAPAELEGLLVSHSEILDAVVIPFPDAE 165
           WVHTGD+GYFDD+GQLFVVDRIKELIKYKGFQVAPAELEGLLVSH +ILDAVVIP+PDAE
Sbjct: 418 WVHTGDVGYFDDDGQLFVVDRIKELIKYKGFQVAPAELEGLLVSHPQILDAVVIPYPDAE 477

Query: 164 AGEVPIAYVVRSPNSSLTEEDVKKFIAEQVAPFKRLRKITFVNSVPKSASGKIL 3
           AGEVP+AYVVRSPNS+LTE DV+KFIA+QVAPFKRLRK+TF+N+VPKSASGKIL
Sbjct: 478 AGEVPVAYVVRSPNSALTEVDVQKFIADQVAPFKRLRKVTFINTVPKSASGKIL 531