BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00014946
         (1412 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002263670.2| PREDICTED: malate dehydrogenase, glyoxysomal...   542   e-152
ref|XP_002310874.1| predicted protein [Populus trichocarpa] gi|2...   533   e-149
pdb|1SEV|A Chain A, Mature And Translocatable Forms Of Glyoxysom...   527   e-147
sp|P19446.1|MDHG_CITLA RecName: Full=Malate dehydrogenase, glyox...   527   e-147
ref|XP_003631692.1| PREDICTED: malate dehydrogenase, glyoxysomal...   526   e-147

>ref|XP_002263670.2| PREDICTED: malate dehydrogenase, glyoxysomal [Vitis vinifera]
            gi|297739396|emb|CBI29427.3| unnamed protein product
            [Vitis vinifera]
          Length = 356

 Score =  542 bits (1397), Expect = e-152
 Identities = 275/327 (84%), Positives = 295/327 (90%)
 Frame = -2

Query: 1369 GLEEVKCRAKGGSPGFKVAILGASGGIGQPLSMLMKMSPLVSVLHLYDVANTHGVTADIS 1190
            GL    CRAKGG+PGFKVAILGA+GGIGQPL+MLMKM+PLVSVLHLYDV NT GVT+DIS
Sbjct: 30   GLSRENCRAKGGAPGFKVAILGAAGGIGQPLAMLMKMNPLVSVLHLYDVVNTPGVTSDIS 89

Query: 1189 HMDTSAVVRGFLGQQQLEDALTGMDLVIIPAGVPRKPGMTRDDLFNINAGIVKTLCEGIA 1010
            HMDT AVVRGFLGQQQLEDALTGMDLVIIPAGVPRKPGMTRDDLFNINAGIVKTLCEGIA
Sbjct: 90   HMDTGAVVRGFLGQQQLEDALTGMDLVIIPAGVPRKPGMTRDDLFNINAGIVKTLCEGIA 149

Query: 1009 KCCPKAVVNVISNPVNSTVPIAAEVFKKAGMFDPRKLLGVTMLDVVRANTFVAEXXXXXX 830
            KCCP A+VN+ISNPVNSTVPIAAEVFKKAG FDP++LLGVTMLDVVRANTFVAE      
Sbjct: 150  KCCPNAIVNLISNPVNSTVPIAAEVFKKAGTFDPKRLLGVTMLDVVRANTFVAEVLGLDP 209

Query: 829  XXXXXXXXXGHAGVTILPLLSQVKPQRSFTPEEINHLTSRIQNGGTEVVEAKAGTGSATL 650
                     GHAGVTILPLLSQVKP  SFTPEEI++LT+RIQNGGTEVVEAKAG GSATL
Sbjct: 210  REVDVPVVGGHAGVTILPLLSQVKPPCSFTPEEIDYLTARIQNGGTEVVEAKAGAGSATL 269

Query: 649  SMAYAAVKFAESCLRGLKGDAGVVQCSFVASQVTELPFFASKVRLGRNGVEEIYPLGPLN 470
            SMAYAAVKFA++CLRGL+GDAGV+QC++V SQVTELPFFASKVRLGR G EEIYPLGPLN
Sbjct: 270  SMAYAAVKFADTCLRGLRGDAGVIQCAYVFSQVTELPFFASKVRLGRTGAEEIYPLGPLN 329

Query: 469  EYERAGLDKAKKELASSISKGIAFARK 389
            EYERAGL+KAKKELASSI KGI+F RK
Sbjct: 330  EYERAGLEKAKKELASSIQKGISFIRK 356


>ref|XP_002310874.1| predicted protein [Populus trichocarpa] gi|222853777|gb|EEE91324.1|
            predicted protein [Populus trichocarpa]
          Length = 354

 Score =  533 bits (1372), Expect = e-149
 Identities = 270/331 (81%), Positives = 294/331 (88%)
 Frame = -2

Query: 1381 ESDWGLEEVKCRAKGGSPGFKVAILGASGGIGQPLSMLMKMSPLVSVLHLYDVANTHGVT 1202
            E D GLE V CRAKG S GFKVAILGA+GGIGQPL+MLMKM+PLVS+LHLYDV N  GVT
Sbjct: 24   EEDLGLERVNCRAKGASSGFKVAILGAAGGIGQPLAMLMKMNPLVSLLHLYDVVNAPGVT 83

Query: 1201 ADISHMDTSAVVRGFLGQQQLEDALTGMDLVIIPAGVPRKPGMTRDDLFNINAGIVKTLC 1022
            ADISHMDTSAVVRGFLGQQQLEDAL GMDLVIIPAGVPRKPGMTRDDLFNINAGIVKTLC
Sbjct: 84   ADISHMDTSAVVRGFLGQQQLEDALIGMDLVIIPAGVPRKPGMTRDDLFNINAGIVKTLC 143

Query: 1021 EGIAKCCPKAVVNVISNPVNSTVPIAAEVFKKAGMFDPRKLLGVTMLDVVRANTFVAEXX 842
            E IAKCCPKA+VN+ISNPVNSTVPIAAEVFKKAG+FDP+++LGVTMLDVVRANTFVAE  
Sbjct: 144  EAIAKCCPKAIVNIISNPVNSTVPIAAEVFKKAGVFDPKRVLGVTMLDVVRANTFVAEIM 203

Query: 841  XXXXXXXXXXXXXGHAGVTILPLLSQVKPQRSFTPEEINHLTSRIQNGGTEVVEAKAGTG 662
                         GHAGVTILPLLSQVKP  SFT +EI++LT RIQNGGTEVVEAKAG G
Sbjct: 204  GLDPREVDVPVVGGHAGVTILPLLSQVKPLCSFTQKEIDYLTDRIQNGGTEVVEAKAGAG 263

Query: 661  SATLSMAYAAVKFAESCLRGLKGDAGVVQCSFVASQVTELPFFASKVRLGRNGVEEIYPL 482
            SATLSMAYAAVKFA++CLR L+GDA VV C++VAS+VTELPFFASKVRLGRNGVEEIYPL
Sbjct: 264  SATLSMAYAAVKFADACLRALRGDAAVVHCAYVASEVTELPFFASKVRLGRNGVEEIYPL 323

Query: 481  GPLNEYERAGLDKAKKELASSISKGIAFARK 389
            GPLN+YERAGL+KAKKELA SI KG++F +K
Sbjct: 324  GPLNDYERAGLEKAKKELAGSIQKGVSFVKK 354


>pdb|1SEV|A Chain A, Mature And Translocatable Forms Of Glyoxysomal Malate
            Dehydrogenase Have Different Activities And Stabilities
            But Similar Crystal Structures gi|60593476|pdb|1SEV|B
            Chain B, Mature And Translocatable Forms Of Glyoxysomal
            Malate Dehydrogenase Have Different Activities And
            Stabilities But Similar Crystal Structures
          Length = 362

 Score =  527 bits (1357), Expect = e-147
 Identities = 264/330 (80%), Positives = 290/330 (87%)
 Frame = -2

Query: 1381 ESDWGLEEVKCRAKGGSPGFKVAILGASGGIGQPLSMLMKMSPLVSVLHLYDVANTHGVT 1202
            E    L    CRAKGG+PGFKVAILGA+GGIGQPL+MLMKM+PLVSVLHLYDV N  GVT
Sbjct: 26   EESSALRRANCRAKGGAPGFKVAILGAAGGIGQPLAMLMKMNPLVSVLHLYDVVNAPGVT 85

Query: 1201 ADISHMDTSAVVRGFLGQQQLEDALTGMDLVIIPAGVPRKPGMTRDDLFNINAGIVKTLC 1022
            ADISHMDT AVVRGFLGQQQLE ALTGMDL+I+PAGVPRKPGMTRDDLF INAGIVKTLC
Sbjct: 86   ADISHMDTGAVVRGFLGQQQLEAALTGMDLIIVPAGVPRKPGMTRDDLFKINAGIVKTLC 145

Query: 1021 EGIAKCCPKAVVNVISNPVNSTVPIAAEVFKKAGMFDPRKLLGVTMLDVVRANTFVAEXX 842
            EGIAKCCP+A+VN+ISNPVNSTVPIAAEVFKKAG +DP++LLGVTMLDVVRANTFVAE  
Sbjct: 146  EGIAKCCPRAIVNLISNPVNSTVPIAAEVFKKAGTYDPKRLLGVTMLDVVRANTFVAEVL 205

Query: 841  XXXXXXXXXXXXXGHAGVTILPLLSQVKPQRSFTPEEINHLTSRIQNGGTEVVEAKAGTG 662
                         GHAGVTILPLLSQVKP  SFT EEI++LT RIQNGGTEVVEAKAG G
Sbjct: 206  GLDPRDVDVPVVGGHAGVTILPLLSQVKPPSSFTQEEISYLTDRIQNGGTEVVEAKAGAG 265

Query: 661  SATLSMAYAAVKFAESCLRGLKGDAGVVQCSFVASQVTELPFFASKVRLGRNGVEEIYPL 482
            SATLSMAYAAVKFA++CLRGL+GDAGV++C+FV+SQVTELPFFASKVRLGRNG+EE+Y L
Sbjct: 266  SATLSMAYAAVKFADACLRGLRGDAGVIECAFVSSQVTELPFFASKVRLGRNGIEEVYSL 325

Query: 481  GPLNEYERAGLDKAKKELASSISKGIAFAR 392
            GPLNEYER GL+KAKKELA SI KG++F R
Sbjct: 326  GPLNEYERIGLEKAKKELAGSIEKGVSFIR 355


>sp|P19446.1|MDHG_CITLA RecName: Full=Malate dehydrogenase, glyoxysomal; Flags: Precursor
            gi|167284|gb|AAA33041.1| glyoxysomal malate dehydrogenase
            precursor (EC 1.1.1.37) [Citrullus lanatus subsp.
            vulgaris]
          Length = 356

 Score =  527 bits (1357), Expect = e-147
 Identities = 264/330 (80%), Positives = 290/330 (87%)
 Frame = -2

Query: 1381 ESDWGLEEVKCRAKGGSPGFKVAILGASGGIGQPLSMLMKMSPLVSVLHLYDVANTHGVT 1202
            E    L    CRAKGG+PGFKVAILGA+GGIGQPL+MLMKM+PLVSVLHLYDV N  GVT
Sbjct: 26   EESSALRRANCRAKGGAPGFKVAILGAAGGIGQPLAMLMKMNPLVSVLHLYDVVNAPGVT 85

Query: 1201 ADISHMDTSAVVRGFLGQQQLEDALTGMDLVIIPAGVPRKPGMTRDDLFNINAGIVKTLC 1022
            ADISHMDT AVVRGFLGQQQLE ALTGMDL+I+PAGVPRKPGMTRDDLF INAGIVKTLC
Sbjct: 86   ADISHMDTGAVVRGFLGQQQLEAALTGMDLIIVPAGVPRKPGMTRDDLFKINAGIVKTLC 145

Query: 1021 EGIAKCCPKAVVNVISNPVNSTVPIAAEVFKKAGMFDPRKLLGVTMLDVVRANTFVAEXX 842
            EGIAKCCP+A+VN+ISNPVNSTVPIAAEVFKKAG +DP++LLGVTMLDVVRANTFVAE  
Sbjct: 146  EGIAKCCPRAIVNLISNPVNSTVPIAAEVFKKAGTYDPKRLLGVTMLDVVRANTFVAEVL 205

Query: 841  XXXXXXXXXXXXXGHAGVTILPLLSQVKPQRSFTPEEINHLTSRIQNGGTEVVEAKAGTG 662
                         GHAGVTILPLLSQVKP  SFT EEI++LT RIQNGGTEVVEAKAG G
Sbjct: 206  GLDPRDVDVPVVGGHAGVTILPLLSQVKPPSSFTQEEISYLTDRIQNGGTEVVEAKAGAG 265

Query: 661  SATLSMAYAAVKFAESCLRGLKGDAGVVQCSFVASQVTELPFFASKVRLGRNGVEEIYPL 482
            SATLSMAYAAVKFA++CLRGL+GDAGV++C+FV+SQVTELPFFASKVRLGRNG+EE+Y L
Sbjct: 266  SATLSMAYAAVKFADACLRGLRGDAGVIECAFVSSQVTELPFFASKVRLGRNGIEEVYSL 325

Query: 481  GPLNEYERAGLDKAKKELASSISKGIAFAR 392
            GPLNEYER GL+KAKKELA SI KG++F R
Sbjct: 326  GPLNEYERIGLEKAKKELAGSIEKGVSFIR 355


>ref|XP_003631692.1| PREDICTED: malate dehydrogenase, glyoxysomal [Vitis vinifera]
            gi|147774268|emb|CAN65552.1| hypothetical protein
            VITISV_033330 [Vitis vinifera]
            gi|297736349|emb|CBI25072.3| unnamed protein product
            [Vitis vinifera]
          Length = 356

 Score =  526 bits (1354), Expect = e-147
 Identities = 266/326 (81%), Positives = 289/326 (88%)
 Frame = -2

Query: 1366 LEEVKCRAKGGSPGFKVAILGASGGIGQPLSMLMKMSPLVSVLHLYDVANTHGVTADISH 1187
            L    CRAKGG+PGFKVAILGA+GGIGQPL+MLMKM+PLVSVLHLYDV N  GVTADISH
Sbjct: 31   LRRANCRAKGGAPGFKVAILGAAGGIGQPLAMLMKMNPLVSVLHLYDVVNAPGVTADISH 90

Query: 1186 MDTSAVVRGFLGQQQLEDALTGMDLVIIPAGVPRKPGMTRDDLFNINAGIVKTLCEGIAK 1007
            MDT AVVRGFLGQ QLE ALTGMDLVIIPAGVPRKPGMTRDDLFNINAGIV+TLCEGIAK
Sbjct: 91   MDTGAVVRGFLGQPQLESALTGMDLVIIPAGVPRKPGMTRDDLFNINAGIVRTLCEGIAK 150

Query: 1006 CCPKAVVNVISNPVNSTVPIAAEVFKKAGMFDPRKLLGVTMLDVVRANTFVAEXXXXXXX 827
            CCP A+VN+ISNPVNSTVPIAAEVFKKAG +DP++LLGVTMLDVVRANTFVAE       
Sbjct: 151  CCPNAIVNLISNPVNSTVPIAAEVFKKAGTYDPKRLLGVTMLDVVRANTFVAEVLGLDPR 210

Query: 826  XXXXXXXXGHAGVTILPLLSQVKPQRSFTPEEINHLTSRIQNGGTEVVEAKAGTGSATLS 647
                    GH+GVTILPLLSQVKP  SFTPEE  +LT+RIQNGGTEVVEAKAG GSATLS
Sbjct: 211  EVDVPVVGGHSGVTILPLLSQVKPPCSFTPEETQYLTNRIQNGGTEVVEAKAGAGSATLS 270

Query: 646  MAYAAVKFAESCLRGLKGDAGVVQCSFVASQVTELPFFASKVRLGRNGVEEIYPLGPLNE 467
            MAYAAVKFA++CLRGL+GDAGVV+C+FVASQVTELPFFA+KVRLGR+G EEIY LGPLNE
Sbjct: 271  MAYAAVKFADACLRGLRGDAGVVECTFVASQVTELPFFATKVRLGRSGAEEIYQLGPLNE 330

Query: 466  YERAGLDKAKKELASSISKGIAFARK 389
            YER GL+KAKKELA SI+KGI+F RK
Sbjct: 331  YERVGLEKAKKELAGSIAKGISFIRK 356