BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00014919
         (1681 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003633713.1| PREDICTED: probable serine/threonine-protein...   449   e-123
ref|XP_002516765.1| wall-associated kinase, putative [Ricinus co...   448   e-123
ref|NP_173275.4| protein kinase domain-containing protein [Arabi...   445   e-122
emb|CBI15487.3| unnamed protein product [Vitis vinifera]              439   e-121
ref|XP_004160450.1| PREDICTED: probable serine/threonine-protein...   420   e-115

>ref|XP_003633713.1| PREDICTED: probable serine/threonine-protein kinase At1g18390-like
            [Vitis vinifera]
          Length = 648

 Score =  449 bits (1155), Expect = e-123
 Identities = 252/535 (47%), Positives = 323/535 (60%), Gaps = 26/535 (4%)
 Frame = -3

Query: 1529 EQYSECLETKFECGQDFVNIGYPFWGINRPSYCGLEGFELTCDKDENYTMIKIESLTFRV 1350
            +Q+  C   ++ECG   +NI YPFWG +RP YCG  GFELTC+ +    +  ++  ++R+
Sbjct: 13   QQFVSC-GAQYECGN--MNISYPFWGGSRPPYCGHPGFELTCNGEA--PVFTMKEASYRI 67

Query: 1349 VSINQSDHDIWITRSTGVEDGADLCPPVVGSSISNRTRFGSSPKSIAVYVYYDCP----- 1185
            + IN S H + + R+   +     CPP   ++  N + F  +     V +YYDCP     
Sbjct: 68   LDINNSFHTLTVARADYWDS---YCPPTYVNTTLNESIFSYNATYTDVTLYYDCPQLTIA 124

Query: 1184 --DNINTSNLTGSLVWDDFKCMKEGVSSNGFYVAESFSESQLQVFKQCKTQLVAPVLRKG 1011
              +  N +N+TG     DF  +   + S   YV        + +FK   T +V+      
Sbjct: 125  PSNQFNCTNITGYYTTLDFN-LGVSIGSCSVYVT-------VPIFKSAATAIVS------ 170

Query: 1010 FDEFRNNATMSLV-EVLRQGFTLNYIIDEVACSACVNSSGLCGSLHSDSKRFQCLCRDGA 834
                    T++L+ E L+ GF L +      C+ CV S G CG     S +F C CR+G 
Sbjct: 171  -----GGGTLTLLTEALKGGFGLEWNASNSLCTECVESGGQCGYT---SNQFTCYCRNGY 222

Query: 833  YPVSCPKHGTKMKVGVKVGIALASLTVGIAFTALACCVHHRRKKFEYGSSL--------- 681
             P +C    +     V +G  L  + +G  F  LA     RR++     S          
Sbjct: 223  SPSTCQNTTSASGYSV-LGAVLLGIFLGCLFYFLA----QRRRRASKAKSKDLPTPPSSK 277

Query: 680  --------ISRDISSYP-ASVDSEKGGTCMGVPIFSYAELEKATNNFDSNREVGDGGFGT 528
                     S  I SYP    D EKG T  GV +FSY ELE+ATNNFD +RE+GDGGFGT
Sbjct: 278  GIAPSLTGFSESIPSYPYTKSDIEKGSTYFGVQVFSYMELEEATNNFDPSRELGDGGFGT 337

Query: 527  VYKGKLQDGRVVAVKRLYENNFKRVEQFMNEIEILAHLRHKNLVPLYGCTSQHCRQLLLV 348
            VY GKLQDGRVVAVKRLYENNFKRVEQFMNE++IL  L+H+NLV LYGCTS+  R+LLLV
Sbjct: 338  VYYGKLQDGRVVAVKRLYENNFKRVEQFMNEVQILTRLQHQNLVKLYGCTSRRSRELLLV 397

Query: 347  YEYVPNGTIADHLYGNLAKPGKFAWKTRMNIATETASALSYLHASDVIHRDVKTTNILLD 168
            YEY+PNGT+ADHL+G  A  G   W  R++IA ETA+AL+YLH S++IHRDVKT NILLD
Sbjct: 398  YEYIPNGTVADHLHGKQANSGSITWPVRLSIAIETANALAYLHESEIIHRDVKTNNILLD 457

Query: 167  NSFSVKVADFGLSRLFPLNATHVSTSPQGTPGYVDPEYHECYQLTDKSDVYSFGV 3
            N+F VKVADFGLSRLFP + THVST+PQGTPGYVDPEYH+CYQLT KSDVYSFGV
Sbjct: 458  NNFQVKVADFGLSRLFPTDVTHVSTAPQGTPGYVDPEYHQCYQLTSKSDVYSFGV 512


>ref|XP_002516765.1| wall-associated kinase, putative [Ricinus communis]
            gi|223544138|gb|EEF45663.1| wall-associated kinase,
            putative [Ricinus communis]
          Length = 685

 Score =  448 bits (1153), Expect = e-123
 Identities = 258/568 (45%), Positives = 333/568 (58%), Gaps = 26/568 (4%)
 Frame = -3

Query: 1628 LEMHLPFFLSKIFVFIIKVLLLSKLQKGHAQDYEQYSECLETKFECGQDFVNIGYPFWGI 1449
            ++  L  F +      I ++L        AQ   QY  C + KFECG +  +IGYPFWG 
Sbjct: 1    MQQRLILFPTMSLFLFITIILFRPPTFACAQIDSQYLNCSQ-KFECG-NITDIGYPFWGS 58

Query: 1448 NRPSYCGLEGFELTCDKDENYTMIKIESLTFRVVSINQSDHDIWITRSTGVEDGADLCPP 1269
            NRP YCG   FEL C       +I IE LT++V+ IN  +  + + R+  +++   +CP 
Sbjct: 59   NRPQYCGHPEFELNCTGQT--AVITIEELTYQVLEINSEEKTLKVARTDYIDN---ICPS 113

Query: 1268 VVGSSISNRTRFGSSPKSIAVYVYYDCPDNINTSNLTGSLVWDDFKCMKEGVSSNGFYVA 1089
               S+  N   F  +     + +YY CP +     L       DF        SNGF+V 
Sbjct: 114  NPVSTTLNFNYFSYTSDIQNITLYYGCPQSNPMPTL------QDFTNQFSCNDSNGFFVT 167

Query: 1088 ESFSE---SQLQVFKQCKTQLVAPVLRKGFDEFRNNATM-SLVEVLRQGFTLNYIIDEVA 921
             + S    + +  F+ C  +++ P  +       N     +LV  L QGF L +  +   
Sbjct: 168  RNLSNLTAALMTYFRTCDVEVIVPANQSAAQSLENRPNQENLVIALEQGFGLEWR-ENST 226

Query: 920  CSACVNSSGLCGSLHSDSKRFQCLCRDGAYPVSCPKHGTKMKVGVKVGIALASLTVGIAF 741
            C  C  S G CGS  +D   F C C +G  P SC   G     GV  GIA  ++ +GI  
Sbjct: 227  CETCNISGGQCGSNSTDPSLFACYCTNGPDPFSC---GRSQSSGV--GIA-GAVVIGIG- 279

Query: 740  TALACCVH-HRRKKFEYGSSLISRDISSYPASV---------------------DSEKGG 627
              L C +  + ++K    +   ++D+ + P+S                      D EKG 
Sbjct: 280  --LGCWIFLYVQRKKRIAAQTKNKDLPTPPSSKSQPAPVINFSQTTPSYSSSKSDLEKGS 337

Query: 626  TCMGVPIFSYAELEKATNNFDSNREVGDGGFGTVYKGKLQDGRVVAVKRLYENNFKRVEQ 447
            T  G  +FSY EL +AT+NFD ++E+GDGGFGTVY G L DGRVVAVKRL+ENN KR EQ
Sbjct: 338  TYFGTKVFSYEELVEATDNFDPSKELGDGGFGTVYYGILSDGRVVAVKRLFENNMKRAEQ 397

Query: 446  FMNEIEILAHLRHKNLVPLYGCTSQHCRQLLLVYEYVPNGTIADHLYGNLAKPGKFAWKT 267
            FMNEIEIL  LRHKNLV LYGCTS+  R+L+LVYEY+PNGT+ADH++GN +K G   WK 
Sbjct: 398  FMNEIEILTRLRHKNLVTLYGCTSKRSRELVLVYEYIPNGTLADHIHGNRSKSGLLTWKV 457

Query: 266  RMNIATETASALSYLHASDVIHRDVKTTNILLDNSFSVKVADFGLSRLFPLNATHVSTSP 87
            R++IA ETA AL+YLHASDVIHRDVKT NILLDN+F VKVADFGLSRLFP + THVST+P
Sbjct: 458  RLSIAIETADALAYLHASDVIHRDVKTNNILLDNNFRVKVADFGLSRLFPNDCTHVSTAP 517

Query: 86   QGTPGYVDPEYHECYQLTDKSDVYSFGV 3
            QGTPGYVDPEY++CYQLTDKSDVYSFGV
Sbjct: 518  QGTPGYVDPEYYQCYQLTDKSDVYSFGV 545


>ref|NP_173275.4| protein kinase domain-containing protein [Arabidopsis thaliana]
            gi|332191588|gb|AEE29709.1| protein kinase
            domain-containing protein [Arabidopsis thaliana]
          Length = 648

 Score =  445 bits (1144), Expect = e-122
 Identities = 257/548 (46%), Positives = 340/548 (62%), Gaps = 17/548 (3%)
 Frame = -3

Query: 1595 IFVFIIKVLLLS--KLQKGHAQDYEQYSECLETKFECGQD---FVNIGYPFWG-INRPSY 1434
            IF F I  ++ S   L    + D +QY EC      CG     F +  YPFWG  N+P +
Sbjct: 3    IFFFFISFVVFSVADLPSCFSAD-QQYEECRSRNLTCGSGHRVFESTTYPFWGGFNKPKF 61

Query: 1433 CGLEGFELTCDKDENYTMIKIESLTFRVVSINQSDHDIWITRSTGVEDGADLCPPVVGSS 1254
            CG   F+L+C+ D+N T+  I ++T RVVS N  DH I +   + ++ G   C  +   +
Sbjct: 62   CGHSSFKLSCEGDQNLTLA-IGNITLRVVSANLEDHKISVADDSLLDGG---CLNIWNFN 117

Query: 1253 ISNRTRFGSSPKSIAVYVYYDCPDNINTSNLTGSLVWDDFKCMKEGVSSNGFYVAESFSE 1074
              N+    S+ ++I V+V        N S +    +     C +       ++V  S   
Sbjct: 118  GKNQFTLDSNTETIDVFV--------NCSGVAPLQI----SCEESYEDPVTYHVLRSSDS 165

Query: 1073 SQLQVFKQCKTQLVAPVLRKGFDEFRNNATMSLVEVLRQGFTLNYIIDEVACSACVNSSG 894
             +      C      P+LR   DE + +  ++ VE LR+GF L YI+++ AC  C++S G
Sbjct: 166  DE-----GCMKYAEIPMLRSAKDELQRSE-LTFVEALRKGFDLRYIMEDKACRRCIDSGG 219

Query: 893  LCGSLHSDSKRFQCLCRDGAYPVSCP------KHGTKMKVGVKVGIALASLTVGIAFTAL 732
            +CGS   DS+ F+CLC D  +  SC       K+  + +V VKV I  ++  VG+   ++
Sbjct: 220  ICGSA-LDSESFRCLCADRPHNSSCDDNTNQGKNDKRRRVIVKVLIGASAAVVGLIAASI 278

Query: 731  ACCVHHRRKKFEY--GSSLISRDISSYPA--SVDSEKGGTCM-GVPIFSYAELEKATNNF 567
               V+HRRK   Y   S+L+ R+ISS P+  S D EK    + GV IFSY ELE+ATNNF
Sbjct: 279  FWYVYHRRKTKSYRNSSALLPRNISSDPSAKSFDIEKAEELLVGVHIFSYEELEEATNNF 338

Query: 566  DSNREVGDGGFGTVYKGKLQDGRVVAVKRLYENNFKRVEQFMNEIEILAHLRHKNLVPLY 387
            D ++E+GDGGFGTVY GKL+DGR VAVKRLY+NNFKR EQF NE+EIL  LRH NLV L+
Sbjct: 339  DPSKELGDGGFGTVYYGKLKDGRSVAVKRLYDNNFKRAEQFRNEVEILTGLRHPNLVALF 398

Query: 386  GCTSQHCRQLLLVYEYVPNGTIADHLYGNLAKPGKFAWKTRMNIATETASALSYLHASDV 207
            GC+S+  R LLLVYEYV NGT+ADHL+G  A P    W  R+ IA ETASAL YLHAS +
Sbjct: 399  GCSSKQSRDLLLVYEYVANGTLADHLHGPQANPSSLPWSIRLKIAVETASALKYLHASKI 458

Query: 206  IHRDVKTTNILLDNSFSVKVADFGLSRLFPLNATHVSTSPQGTPGYVDPEYHECYQLTDK 27
            IHRDVK+ NILLD +F+VKVADFGLSRLFP++ THVST+PQGTPGYVDP+YH CYQL++K
Sbjct: 459  IHRDVKSNNILLDQNFNVKVADFGLSRLFPMDKTHVSTAPQGTPGYVDPDYHLCYQLSNK 518

Query: 26   SDVYSFGV 3
            SDVYSF V
Sbjct: 519  SDVYSFAV 526


>emb|CBI15487.3| unnamed protein product [Vitis vinifera]
          Length = 973

 Score =  439 bits (1130), Expect = e-121
 Identities = 247/497 (49%), Positives = 319/497 (64%), Gaps = 6/497 (1%)
 Frame = -3

Query: 1475 NIGYPFW-GINRPSYCGLEGFELTCDKDENYTMIKIESLTFRVVSINQSDHDIWITRSTG 1299
            N+ YPFW    + SYCG   F +TC+ ++    I  +    R   I  ++H   ++ S  
Sbjct: 361  NVSYPFWIPKEQESYCGFPNFSITCNDEKPVLTISDDYYVIR--EIFYTNHSFLMSNSA- 417

Query: 1298 VEDGADLCPPVVGSSISNRTRFGSSPKSIAVYVYYDCPDNINTSNLTGSLVWDDFKCMKE 1119
            V DG D CP  + +   +RT F  SP    + ++Y+C  ++    LT  +      CM  
Sbjct: 418  VYDG-DSCPTPLHNFSLDRTPFNYSPSHYDLLLFYNCT-SVPVETLTIPI-----DCM-- 468

Query: 1118 GVSSNGFYVAESFSESQLQVF----KQCKTQLVAPVLRKGFDEFRNNATMSLVEVLRQGF 951
              S+   +   SF E  L+      + C++ +  PV   G + F N    +  E+LR+GF
Sbjct: 469  --SNATLHSFASFHEEALEYMNFSTESCQSMVNVPVDIDGEEGFGNLLNWNFTEILREGF 526

Query: 950  TLNYIIDEVACSACVNSSGLCGSLHSDSKRFQCLCRDGAYPVSCPKHGTKMKVGVKVGIA 771
             L++  +   CS+C  S G CG    ++  F C C+D  +  +C   G      V +G+ 
Sbjct: 527  VLSWTANN--CSSCERSGGRCGF---ENNEFICFCQDRPHLRTCHDEGLNYHRKVIIGVC 581

Query: 770  LASLTVGIAFTALACCVHHRRKKFEYGSSLISRDISSYPASVDS-EKGGTCMGVPIFSYA 594
                T+ I+  ++   ++ RR K  Y   L SR+ISS P+S    E  G+  GV IF+Y 
Sbjct: 582  AGLGTLLIS--SIFFLMYLRRYKKRYPPPLFSRNISSDPSSKTIFESQGSLHGVHIFTYE 639

Query: 593  ELEKATNNFDSNREVGDGGFGTVYKGKLQDGRVVAVKRLYENNFKRVEQFMNEIEILAHL 414
            ELE+ATNNFDS++E+GDGGFGTVY GKL+DGRVVAVKRLYENN+KRVEQFMNE+EIL  L
Sbjct: 640  ELEEATNNFDSSKELGDGGFGTVYHGKLRDGRVVAVKRLYENNYKRVEQFMNEVEILQLL 699

Query: 413  RHKNLVPLYGCTSQHCRQLLLVYEYVPNGTIADHLYGNLAKPGKFAWKTRMNIATETASA 234
            RH+NLV LYGCTS+H R+LLLVYEYVPNGT+ADHL+G  AKPG   W TRM IA ETASA
Sbjct: 700  RHRNLVSLYGCTSRHSRELLLVYEYVPNGTVADHLHGEQAKPGSLTWPTRMKIAIETASA 759

Query: 233  LSYLHASDVIHRDVKTTNILLDNSFSVKVADFGLSRLFPLNATHVSTSPQGTPGYVDPEY 54
            L YLHASD+IHRDVKT NILLD++FSVKVADFGLSRLFP + THVST+PQGTPGYVDP+Y
Sbjct: 760  LKYLHASDIIHRDVKTNNILLDSNFSVKVADFGLSRLFPTDVTHVSTAPQGTPGYVDPDY 819

Query: 53   HECYQLTDKSDVYSFGV 3
            H+CYQLT KSDVYSFGV
Sbjct: 820  HQCYQLTSKSDVYSFGV 836



 Score =  150 bits (379), Expect = 1e-33
 Identities = 88/255 (34%), Positives = 129/255 (50%), Gaps = 10/255 (3%)
 Frame = -3

Query: 1535 DYEQYSECLETKFECGQDFVNIGYPFWGINRPSYCGLEGFELTCDKDENYTMIKIESLTF 1356
            D +QY EC  + +ECG D   IGYPFWG  RP +CG +GFEL C  D+ Y +I I SL F
Sbjct: 32   DNQQYEECTSSLYECG-DMKGIGYPFWGDGRPKFCGQQGFELKCQNDD-YPLIDIGSLEF 89

Query: 1355 RVVSINQSDHDIWITRSTGVEDGADLCPPVVGSSISNRTRFGSSPKSIAVYVYYDCPDNI 1176
            RV++I+ S + + I R    +     CP    S+  N T F  +     + ++Y CPD++
Sbjct: 90   RVLNISNSTYAMRIARK---DFWDQTCPKEFQSTTLNYTLFDYAGTDRNLTLFYGCPDDV 146

Query: 1175 NTSNLTGSLVWDDFKCMKEGVS-SNGFYVAESF--SESQLQVFKQCKTQLVAPVLRKGFD 1005
             +       + ++F C  E ++ +  FY  E+F   +  L + + CK  +  PVL    D
Sbjct: 147  LSQLPATWNISNNFTCSVEDINDTTAFYADEAFLGLDQHLAILRTCKINVTLPVLGAAID 206

Query: 1004 EFRNNAT-------MSLVEVLRQGFTLNYIIDEVACSACVNSSGLCGSLHSDSKRFQCLC 846
            E   N T         L + +  GF ++Y +    C  C  + G+CGS  S +  F CLC
Sbjct: 207  ELNANRTGGEFSNGERLEKAMNMGFDVDYSVVRGLCVHCNTTGGICGS--SSTSSFSCLC 264

Query: 845  RDGAYPVSCPKHGTK 801
            RD +YP SC K   K
Sbjct: 265  RDKSYPYSCQKSEAK 279


>ref|XP_004160450.1| PREDICTED: probable serine/threonine-protein kinase At1g18390-like
            [Cucumis sativus]
          Length = 668

 Score =  420 bits (1079), Expect = e-115
 Identities = 236/515 (45%), Positives = 314/515 (60%), Gaps = 18/515 (3%)
 Frame = -3

Query: 1493 CGQDFVNIGYPFW--GINRPSYCGLEGFELTCDKDENYTMIKIESLTFRVVSINQSDHDI 1320
            CG   V I YPFW  G+ + S CG   F++ C +D  Y +++I   TF V  I       
Sbjct: 37   CGHGPV-IKYPFWIDGV-QDSSCGYPDFKIKCSRD--YPVLEISDDTFIVEDIFHQTKSF 92

Query: 1319 WITRSTGVEDGADLCPPVVGSSISNRTRFGSSPKSIAVYVYYDCPDNINTSNLTGSLVWD 1140
             +  +   +D      P  G  +  +  F  S ++     +YDCP            V+ 
Sbjct: 93   LLVSAAAYDDPHSCPSPSHGIRLDGKP-FHFSSENEDFSFFYDCPK-----------VYK 140

Query: 1139 DFKCMKEGVSSNGFYVAESFSESQLQV----FKQCKTQLVAPVLRKGFDEFRNNATMSLV 972
            D+       ++   +   +F +  L+      + C++ +  P+ +       +   MS V
Sbjct: 141  DYIYELSCTNNTTHFSFATFHKDLLEFQNFPLQSCRSSVHVPIRKNLTASVADLREMSYV 200

Query: 971  EVLRQGFTLNYIIDEVACSACVNSSGLCGSLHSDSKRFQCLCRDGAYPVSCPKHGTKMKV 792
            E+ ++GF LN+   +  CS C  S G C     ++ +F C C DG +  SC KHG + + 
Sbjct: 201  EIWKRGFFLNWNAQD--CSKCEQSGGYC---RLENNKFACSCSDGLHSNSC-KHGNRTRT 254

Query: 791  GVKVGIALASLTVGIAFTALACCVHHR--RKKFEYGSSLISRDISSYPASVDS------- 639
             + +G+      + + F  L  C   R  R++  +    + R IS  P++  +       
Sbjct: 255  KIIIGVCSGVGALLLTFLVLVICYRWRQLRRRRSHALPYVQRSISLNPSNPSNPPNPSPV 314

Query: 638  ---EKGGTCMGVPIFSYAELEKATNNFDSNREVGDGGFGTVYKGKLQDGRVVAVKRLYEN 468
               E GGT +GV +FSY ELE+ATN+FDSN+E+GDGGFGTVY G L+DGR VAVKRL+E+
Sbjct: 315  EEVENGGTYLGVHLFSYKELEEATNHFDSNKELGDGGFGTVYFGLLKDGRAVAVKRLFES 374

Query: 467  NFKRVEQFMNEIEILAHLRHKNLVPLYGCTSQHCRQLLLVYEYVPNGTIADHLYGNLAKP 288
            NFKRVEQFMNE+EILA LRH+NLV LYGCTS+  R+LLLVYEYVPNGT+ADHL+G LAK 
Sbjct: 375  NFKRVEQFMNEVEILARLRHRNLVSLYGCTSRSSRELLLVYEYVPNGTVADHLHGKLAKS 434

Query: 287  GKFAWKTRMNIATETASALSYLHASDVIHRDVKTTNILLDNSFSVKVADFGLSRLFPLNA 108
            GK  W TRM IA ETASAL YLHAS++IHRDVKT NILLDN++ VKVADFGLSRLFPL+ 
Sbjct: 435  GKLPWCTRMKIAIETASALVYLHASEIIHRDVKTNNILLDNNYCVKVADFGLSRLFPLDV 494

Query: 107  THVSTSPQGTPGYVDPEYHECYQLTDKSDVYSFGV 3
            THVST+PQGTPGYVDPEYH+CYQL+DKSDV+SFGV
Sbjct: 495  THVSTAPQGTPGYVDPEYHQCYQLSDKSDVFSFGV 529