BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00014781
         (759 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAD30038.1| gibberellin 2-oxidase1 [Daucus carota]                183   6e-89
ref|XP_002305704.1| gibberellin 2-oxidase [Populus trichocarpa] ...   150   5e-66
gb|ABS19663.1| gibberellin 2-oxidase 1 [Solanum tuberosum]            144   2e-64
gb|AFK39291.1| unknown [Lotus japonicus]                              137   2e-64
gb|AEA51242.1| gibberellin 2-beta-dioxygenase [Prunus salicina] ...   150   6e-64

>dbj|BAD30038.1| gibberellin 2-oxidase1 [Daucus carota]
          Length = 317

 Score =  183 bits (464), Expect(2) = 6e-89
 Identities = 90/114 (78%), Positives = 102/114 (89%)
 Frame = +1

Query: 337 DCRVMVSKYLSAIKKMAFEVLELMADGLELDEPRNVLSRLLSSEKSDSFFRINHYPASDK 516
           D   MV+KYLSA+K +A E+L+LMA+ L L +P+NVLSRLLS +KSDSFFRINHYPASD+
Sbjct: 125 DFWAMVNKYLSAVKNLACEILKLMAEELNL-QPKNVLSRLLSDKKSDSFFRINHYPASDR 183

Query: 517 NELGFGEHTDPQVISVIRSNNISGLEIALKDGTWVQVPPDPNSFFITVDDCLQV 678
           NELGFGEHTDPQ+ISVIRSNN SGLEIALKDGTW QVP DP+SFFITVDDCLQV
Sbjct: 184 NELGFGEHTDPQIISVIRSNNASGLEIALKDGTWTQVPADPSSFFITVDDCLQV 237



 Score =  171 bits (433), Expect(2) = 6e-89
 Identities = 86/107 (80%), Positives = 92/107 (85%)
 Frame = +3

Query: 15  TTHEIPVVDLAHPDAKTLIVNACQEFGFFKVTNHGVPMDTVTNLEAQALSFFKLPQSQKN 194
           +TH IPVVDL++PDAKT IVNACQEFGFFKV NHGVPM+ VT LEAQALSFFK PQ+ KN
Sbjct: 23  STH-IPVVDLSNPDAKTQIVNACQEFGFFKVINHGVPMEIVTKLEAQALSFFKQPQNHKN 81

Query: 195 KAAPFGYGNKNIGRKGDTGWVDYLLFNTDINIQLNSHNSLNILPQDF 335
           KAAPFGYGNKNIGRKGDTGWV+YLLF TD   QL S NSL ILP DF
Sbjct: 82  KAAPFGYGNKNIGRKGDTGWVEYLLFGTD--TQLISQNSLTILPNDF 126


>ref|XP_002305704.1| gibberellin 2-oxidase [Populus trichocarpa]
           gi|222848668|gb|EEE86215.1| gibberellin 2-oxidase
           [Populus trichocarpa]
          Length = 332

 Score =  150 bits (378), Expect(2) = 5e-66
 Identities = 78/119 (65%), Positives = 89/119 (74%), Gaps = 7/119 (5%)
 Frame = +1

Query: 343 RVMVSKYLSAIKKMAFEVLELMADGLELDEPRNVLSRLLSSEKSDSFFRINHYPA----- 507
           R  V  Y+ A+K+MAFEVLELMADGLE+ E RNV SRLL  +KSDS FR+NHYP      
Sbjct: 133 RSAVEDYILAVKRMAFEVLELMADGLEI-ESRNVFSRLLRDDKSDSCFRLNHYPPCSELQ 191

Query: 508 --SDKNELGFGEHTDPQVISVIRSNNISGLEIALKDGTWVQVPPDPNSFFITVDDCLQV 678
             S  N +GFGEHTDPQ+ISV+RSNN SGL+I LK+GTWV VPPD  SFFI V D LQV
Sbjct: 192 ALSGGNLIGFGEHTDPQIISVLRSNNTSGLQICLKEGTWVSVPPDQTSFFINVGDALQV 250



 Score =  127 bits (320), Expect(2) = 5e-66
 Identities = 62/107 (57%), Positives = 74/107 (69%), Gaps = 3/107 (2%)
 Frame = +3

Query: 24  EIPVVDLAHPDAKTLIVNACQEFGFFKVTNHGVPMDTVTNLEAQALSFFKLPQSQKNKAA 203
           EIPV+DL  P AKTLI+ AC+EFGFFK+ NHGVPM+ +T LEA A +FF LPQ +K+KA 
Sbjct: 26  EIPVIDLTDPHAKTLIIKACEEFGFFKLVNHGVPMEVMTKLEALATNFFNLPQPEKDKAG 85

Query: 204 ---PFGYGNKNIGRKGDTGWVDYLLFNTDINIQLNSHNSLNILPQDF 335
              PFGYGNK IG  GD GWV+YLL NT+  I     +     PQ F
Sbjct: 86  PPNPFGYGNKKIGPNGDVGWVEYLLLNTNPQISSQKTSIFQENPQIF 132


>gb|ABS19663.1| gibberellin 2-oxidase 1 [Solanum tuberosum]
          Length = 340

 Score =  144 bits (364), Expect(2) = 2e-64
 Identities = 72/117 (61%), Positives = 90/117 (76%), Gaps = 8/117 (6%)
 Frame = +1

Query: 349 MVSKYLSAIKKMAFEVLELMADGLELDEPRNVLSRLLSSEKSDSFFRINHYPA------- 507
           +V +Y+SA++ M   VLE++A+GL + EP+N+LSR+LS EKSDS FR+NHYP        
Sbjct: 141 LVKEYISAVRNMGCMVLEMIAEGLNI-EPKNILSRMLSDEKSDSCFRLNHYPPCPELLQA 199

Query: 508 -SDKNELGFGEHTDPQVISVIRSNNISGLEIALKDGTWVQVPPDPNSFFITVDDCLQ 675
            S +N +GFGEHTDPQVISV RSNN SGL+I+LKDGTW+ VPPDP SFFI V D LQ
Sbjct: 200 LSGRNLIGFGEHTDPQVISVGRSNNTSGLQISLKDGTWISVPPDPYSFFINVGDSLQ 256



 Score =  128 bits (321), Expect(2) = 2e-64
 Identities = 62/109 (56%), Positives = 79/109 (72%), Gaps = 3/109 (2%)
 Frame = +3

Query: 3   NSHFTTHEIPVVDLAHPDAKTLIVNACQEFGFFKVTNHGVPMDTVTNLEAQALSFFKLPQ 182
           + H  T  IPV+DL  P+A  LI+ ACQEFGFFKV NHGVP++T+T LE ++++FF L Q
Sbjct: 25  SDHVYTGIIPVIDLLDPEANNLIIKACQEFGFFKVVNHGVPIETITKLENESINFFNLSQ 84

Query: 183 SQKNK---AAPFGYGNKNIGRKGDTGWVDYLLFNTDINIQLNSHNSLNI 320
            +K+K   A PFGYGNK IG KGD GWV+YLLF T  N +LN + S+ I
Sbjct: 85  IEKDKVGPANPFGYGNKRIGSKGDVGWVEYLLFTT--NPELNHNKSITI 131


>gb|AFK39291.1| unknown [Lotus japonicus]
          Length = 336

 Score =  137 bits (345), Expect(2) = 2e-64
 Identities = 68/118 (57%), Positives = 86/118 (72%), Gaps = 9/118 (7%)
 Frame = +1

Query: 352 VSKYLSAIKKMAFEVLELMADGLELDEPRNVLSRLLSSEKSDSFFRINHYPA-------- 507
           V+ Y  A+K ++ E+LELMADGL +++ RNV +RL+  EKSD  FR+NHYPA        
Sbjct: 138 VNDYTLAVKNLSCEILELMADGLGIEQ-RNVFNRLIRDEKSDCIFRLNHYPACGELELQA 196

Query: 508 -SDKNELGFGEHTDPQVISVIRSNNISGLEIALKDGTWVQVPPDPNSFFITVDDCLQV 678
              +N +GFGEHTDPQ+ISV+RSNN SGL+I L+DGTWV +PPD  SFFI V D LQV
Sbjct: 197 IGGRNLIGFGEHTDPQMISVLRSNNTSGLQICLRDGTWVSIPPDQTSFFINVGDSLQV 254



 Score =  135 bits (339), Expect(2) = 2e-64
 Identities = 65/106 (61%), Positives = 83/106 (78%), Gaps = 3/106 (2%)
 Frame = +3

Query: 24  EIPVVDLAHPDAKTLIVNACQEFGFFKVTNHGVPMDTVTNLEAQALSFFKLPQSQKNKAA 203
           E+P VDL+HP+AKTLIVNAC+EFGFFK+ NH +PM+ ++NLE +ALSFF  PQS+K KA+
Sbjct: 27  EVPEVDLSHPEAKTLIVNACKEFGFFKIINHDIPMELISNLENEALSFFMQPQSEKEKAS 86

Query: 204 ---PFGYGNKNIGRKGDTGWVDYLLFNTDINIQLNSHNSLNILPQD 332
              PFGYG+K IG+ GD GWV+YLLFNT  N  + S N L +L Q+
Sbjct: 87  LDDPFGYGSKRIGKNGDVGWVEYLLFNT--NPDVISPNPLLLLEQN 130


>gb|AEA51242.1| gibberellin 2-beta-dioxygenase [Prunus salicina]
           gi|327359297|gb|AEA51243.1| gibberellin
           2-beta-dioxygenase [Prunus salicina]
          Length = 342

 Score =  150 bits (379), Expect(2) = 6e-64
 Identities = 77/119 (64%), Positives = 89/119 (74%), Gaps = 7/119 (5%)
 Frame = +1

Query: 343 RVMVSKYLSAIKKMAFEVLELMADGLELDEPRNVLSRLLSSEKSDSFFRINHYPASD--- 513
           R  V  Y+ A+KKM FEVLELM DGL + EPRNVLS LL  +KSD  FR+N+YP      
Sbjct: 136 RDAVLDYICAVKKMTFEVLELMVDGLGI-EPRNVLSNLLREDKSDCCFRLNYYPPCPELQ 194

Query: 514 ----KNELGFGEHTDPQVISVIRSNNISGLEIALKDGTWVQVPPDPNSFFITVDDCLQV 678
               +N +GFGEHTDPQ+ISV+RSNN SGL+I+LKDGTWV VPPD NSFFI V DCLQV
Sbjct: 195 ALRGRNLIGFGEHTDPQIISVLRSNNTSGLQISLKDGTWVSVPPDQNSFFINVGDCLQV 253



 Score =  120 bits (301), Expect(2) = 6e-64
 Identities = 59/105 (56%), Positives = 76/105 (72%), Gaps = 3/105 (2%)
 Frame = +3

Query: 27  IPVVDLAHPDAKTLIVNACQEFGFFKVTNHGVPMDTVTNLEAQALSFFKLPQSQKNKAA- 203
           IPVVDL+ P+AK  IV AC+++G FK+ NHGVP+D +T LEAQAL FF LPQS+K KA  
Sbjct: 29  IPVVDLSDPEAKHHIVKACEDYGLFKLVNHGVPLDFMTTLEAQALKFFNLPQSEKEKAGP 88

Query: 204 --PFGYGNKNIGRKGDTGWVDYLLFNTDINIQLNSHNSLNILPQD 332
             PFGYG+K IG  GD GW++Y+L NT  N  + S  SL+I  ++
Sbjct: 89  AEPFGYGSKRIGPNGDVGWIEYILLNT--NPDIISPKSLSIFKEN 131