BLASTX nr result
ID: Bupleurum21_contig00014537
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00014537
(1356 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_003550013.1| PREDICTED: spindle assembly checkpoint compo... 240 7e-61
emb|CBI29639.3| unnamed protein product [Vitis vinifera] 238 3e-60
ref|XP_002269253.1| PREDICTED: uncharacterized protein LOC100262... 238 3e-60
ref|XP_002527831.1| Spindle assembly checkpoint component mad1, ... 237 5e-60
ref|XP_003529697.1| PREDICTED: spindle assembly checkpoint compo... 233 7e-59
>ref|XP_003550013.1| PREDICTED: spindle assembly checkpoint component mad1-like [Glycine
max]
Length = 703
Score = 240 bits (612), Expect = 7e-61
Identities = 128/237 (54%), Positives = 166/237 (70%)
Frame = +3
Query: 645 KARLLEGQLRSLSESTEREKARLNQELIQMKKEAKLSISRMSTNLERMECRAKSAEEESG 824
KA LEG+L LSES EREK RL+ E Q+K ++ LSISR+S NLE+MECRA +AE+E+
Sbjct: 156 KAASLEGKLGHLSESIEREKKRLHDEHSQLKSDSNLSISRISANLEQMECRANNAEKEAE 215
Query: 825 MXXXXXXXXXXXXXXCLQQKNEVEKKLSNSMFQGVASTDATILVKHLQEELKNYESEVCE 1004
+ CL QK EVEKKLS M Q VAST++ +LVKHLQ+EL+NYESEV E
Sbjct: 216 LLKEQLKHLKDQLDECLHQKIEVEKKLSTLMSQEVASTESNVLVKHLQQELRNYESEVRE 275
Query: 1005 AKRLKSSHEDIXXXXXXXXXXXXXXXXAELDLLKLQDVHLSTTKLEDELSAWKSLLQQIP 1184
A++L SSHE+I AE +L KLQD+ L+ KLED++S+W+ ++ IP
Sbjct: 276 ARKLSSSHENIELLKEKILEEKSHRERAESELSKLQDIQLNMKKLEDQISSWRVMITDIP 335
Query: 1185 GVSCADDVPLKFASLQKEVVNGMMKLGETAERLKKLEVALEAAEVGKQNAEADTILA 1355
GVSC +D+P+KFA+LQKEV+ G K GE RLK++EVAL+AAE+GKQNAEA+ LA
Sbjct: 336 GVSCFEDLPVKFAALQKEVIYGTQKEGEITARLKQMEVALDAAEIGKQNAEAEAELA 392
Score = 151 bits (381), Expect = 4e-34
Identities = 80/126 (63%), Positives = 93/126 (73%)
Frame = +1
Query: 73 MIVRTPPLKRKAETSAPGSPNSDQRLIIFEDLPAPEPSHNPSEHMLCTYQCRQMVKSDFF 252
MIVRTPP ++ P + D +L+I+ED P P PSE MLCTYQCRQMVKSDF
Sbjct: 1 MIVRTPPPPKR-----PRADGDDGQLVIYEDPPESSP---PSEQMLCTYQCRQMVKSDFI 52
Query: 253 DALNSAEKQARDSQSQLEALSDNFGKTESERKRFRDQFLYAEQELAAAKGREQALQDQLM 432
DAL+ AE QAR QS+ E L +F K ESERK+F+DQFLYA+QELAAAKGREQALQDQL+
Sbjct: 53 DALSKAENQARHYQSKFETLEPHFRKVESERKKFQDQFLYAQQELAAAKGREQALQDQLL 112
Query: 433 KEANDS 450
KEA S
Sbjct: 113 KEATQS 118
>emb|CBI29639.3| unnamed protein product [Vitis vinifera]
Length = 441
Score = 238 bits (606), Expect = 3e-60
Identities = 130/238 (54%), Positives = 164/238 (68%), Gaps = 1/238 (0%)
Frame = +3
Query: 645 KARLLEGQLRSLSESTEREKARLNQELIQMKKEAKLSISRMSTNLERMECRAKSAEEESG 824
KA LEG+L SES EREK RL EL Q+K+E+KLS+SR+S +LERMECRA +AE+ES
Sbjct: 165 KASALEGKLSQFSESIEREKKRLQYELAQLKRESKLSVSRISADLERMECRANNAEKESE 224
Query: 825 MXXXXXXXXXXXXXXCLQQKNEVEKKLSNSMFQGVA-STDATILVKHLQEELKNYESEVC 1001
+ CL QK+E EKKLS+ Q V S ++ ILVKHLQEEL+NY EV
Sbjct: 225 LLKEQLEELKSQLNECLHQKSEAEKKLSSCTSQEVTTSMESDILVKHLQEELRNYGFEVR 284
Query: 1002 EAKRLKSSHEDIXXXXXXXXXXXXXXXXAELDLLKLQDVHLSTTKLEDELSAWKSLLQQI 1181
EA++LKSSHE+I AE +LLKL ++ LS KLEDEL +WK +++ I
Sbjct: 285 EARKLKSSHENIELLKEKLLEEKGRRERAESELLKLPEIQLSMKKLEDELLSWKLMVKDI 344
Query: 1182 PGVSCADDVPLKFASLQKEVVNGMMKLGETAERLKKLEVALEAAEVGKQNAEADTILA 1355
PGVSC+DDVP+KFA+LQKEV+ GMMKLGE RLK++EV+L+AAE KQNAE + A
Sbjct: 345 PGVSCSDDVPMKFAALQKEVIEGMMKLGEANARLKQMEVSLDAAEHAKQNAETEAASA 402
Score = 175 bits (444), Expect = 2e-41
Identities = 89/128 (69%), Positives = 108/128 (84%), Gaps = 2/128 (1%)
Frame = +1
Query: 73 MIVRTPP-LKRKAETS-APGSPNSDQRLIIFEDLPAPEPSHNPSEHMLCTYQCRQMVKSD 246
MI+RTPP KR+A+ S AP SP SD+RL+I+ED P PE SH PSE MLCTYQCRQMVK++
Sbjct: 1 MILRTPPPRKRRADDSRAPESPGSDRRLVIYED-PVPESSHGPSEQMLCTYQCRQMVKAE 59
Query: 247 FFDALNSAEKQARDSQSQLEALSDNFGKTESERKRFRDQFLYAEQELAAAKGREQALQDQ 426
F ++LNSAEKQ RD QS+LEA ++NF K E++RK+FRDQF YAEQELAA KGRE+ALQ+Q
Sbjct: 60 FLESLNSAEKQVRDYQSRLEASNENFCKAEADRKKFRDQFFYAEQELAAVKGREKALQEQ 119
Query: 427 LMKEANDS 450
L+KE NDS
Sbjct: 120 LLKEVNDS 127
>ref|XP_002269253.1| PREDICTED: uncharacterized protein LOC100262667 [Vitis vinifera]
Length = 717
Score = 238 bits (606), Expect = 3e-60
Identities = 130/238 (54%), Positives = 164/238 (68%), Gaps = 1/238 (0%)
Frame = +3
Query: 645 KARLLEGQLRSLSESTEREKARLNQELIQMKKEAKLSISRMSTNLERMECRAKSAEEESG 824
KA LEG+L SES EREK RL EL Q+K+E+KLS+SR+S +LERMECRA +AE+ES
Sbjct: 165 KASALEGKLSQFSESIEREKKRLQYELAQLKRESKLSVSRISADLERMECRANNAEKESE 224
Query: 825 MXXXXXXXXXXXXXXCLQQKNEVEKKLSNSMFQGVA-STDATILVKHLQEELKNYESEVC 1001
+ CL QK+E EKKLS+ Q V S ++ ILVKHLQEEL+NY EV
Sbjct: 225 LLKEQLEELKSQLNECLHQKSEAEKKLSSCTSQEVTTSMESDILVKHLQEELRNYGFEVR 284
Query: 1002 EAKRLKSSHEDIXXXXXXXXXXXXXXXXAELDLLKLQDVHLSTTKLEDELSAWKSLLQQI 1181
EA++LKSSHE+I AE +LLKL ++ LS KLEDEL +WK +++ I
Sbjct: 285 EARKLKSSHENIELLKEKLLEEKGRRERAESELLKLPEIQLSMKKLEDELLSWKLMVKDI 344
Query: 1182 PGVSCADDVPLKFASLQKEVVNGMMKLGETAERLKKLEVALEAAEVGKQNAEADTILA 1355
PGVSC+DDVP+KFA+LQKEV+ GMMKLGE RLK++EV+L+AAE KQNAE + A
Sbjct: 345 PGVSCSDDVPMKFAALQKEVIEGMMKLGEANARLKQMEVSLDAAEHAKQNAETEAASA 402
Score = 175 bits (444), Expect = 2e-41
Identities = 89/128 (69%), Positives = 108/128 (84%), Gaps = 2/128 (1%)
Frame = +1
Query: 73 MIVRTPP-LKRKAETS-APGSPNSDQRLIIFEDLPAPEPSHNPSEHMLCTYQCRQMVKSD 246
MI+RTPP KR+A+ S AP SP SD+RL+I+ED P PE SH PSE MLCTYQCRQMVK++
Sbjct: 1 MILRTPPPRKRRADDSRAPESPGSDRRLVIYED-PVPESSHGPSEQMLCTYQCRQMVKAE 59
Query: 247 FFDALNSAEKQARDSQSQLEALSDNFGKTESERKRFRDQFLYAEQELAAAKGREQALQDQ 426
F ++LNSAEKQ RD QS+LEA ++NF K E++RK+FRDQF YAEQELAA KGRE+ALQ+Q
Sbjct: 60 FLESLNSAEKQVRDYQSRLEASNENFCKAEADRKKFRDQFFYAEQELAAVKGREKALQEQ 119
Query: 427 LMKEANDS 450
L+KE NDS
Sbjct: 120 LLKEVNDS 127
>ref|XP_002527831.1| Spindle assembly checkpoint component mad1, putative [Ricinus
communis] gi|223532755|gb|EEF34534.1| Spindle assembly
checkpoint component mad1, putative [Ricinus communis]
Length = 728
Score = 237 bits (605), Expect = 5e-60
Identities = 122/237 (51%), Positives = 169/237 (71%)
Frame = +3
Query: 645 KARLLEGQLRSLSESTEREKARLNQELIQMKKEAKLSISRMSTNLERMECRAKSAEEESG 824
KA +LEG+L LSES E+EK RLN EL+Q+++++KLS+SR++ + E+MECRAK+AE+ES
Sbjct: 177 KASVLEGKLAHLSESIEKEKKRLNNELVQLQRDSKLSVSRITADHEKMECRAKNAEKESE 236
Query: 825 MXXXXXXXXXXXXXXCLQQKNEVEKKLSNSMFQGVASTDATILVKHLQEELKNYESEVCE 1004
+ CL QK E+EKKLS+ Q +ST+ ILVKHLQEEL+N ESEV E
Sbjct: 237 LLKAQLEDLKLQLSECLHQKGELEKKLSSFAIQEGSSTEGNILVKHLQEELRNCESEVRE 296
Query: 1005 AKRLKSSHEDIXXXXXXXXXXXXXXXXAELDLLKLQDVHLSTTKLEDELSAWKSLLQQIP 1184
A++LKSS+E++ AE +L K Q++ L+ LEDELS+WKSL+++IP
Sbjct: 297 ARKLKSSYENVELLKEKLLEEKSRRERAESELSKFQELELNMRNLEDELSSWKSLIKEIP 356
Query: 1185 GVSCADDVPLKFASLQKEVVNGMMKLGETAERLKKLEVALEAAEVGKQNAEADTILA 1355
VSC DD+PLKFA+LQKE+++ MMK+GE R+K++EVAL+AA +GKQNAE + A
Sbjct: 357 NVSCCDDIPLKFAALQKEMIDNMMKVGEANARVKQMEVALDAAHLGKQNAETEVASA 413
Score = 163 bits (413), Expect = 8e-38
Identities = 90/140 (64%), Positives = 108/140 (77%), Gaps = 14/140 (10%)
Frame = +1
Query: 73 MIVRTPPLKR-----KAETSAPGSPNSDQRLIIFEDLPA-----PEPS----HNPSEHML 210
MI+RTPP KR KA ++P + Q LII+ED PA P+ S H PS+HML
Sbjct: 1 MILRTPPPKRPRDDVKAIETSPVGSDRRQPLIIYED-PAVIVQHPDDSSHEPHQPSDHML 59
Query: 211 CTYQCRQMVKSDFFDALNSAEKQARDSQSQLEALSDNFGKTESERKRFRDQFLYAEQELA 390
CTYQCRQMVKSDFFDAL++AEKQA D QS+LE L++NF K ++ERK+FRDQFLYAEQELA
Sbjct: 60 CTYQCRQMVKSDFFDALSNAEKQASDYQSKLETLNENFSKADAERKKFRDQFLYAEQELA 119
Query: 391 AAKGREQALQDQLMKEANDS 450
AAKGRE+ALQ+QL+KE NDS
Sbjct: 120 AAKGREKALQEQLLKEINDS 139
>ref|XP_003529697.1| PREDICTED: spindle assembly checkpoint component mad1-like [Glycine
max]
Length = 701
Score = 233 bits (595), Expect = 7e-59
Identities = 123/237 (51%), Positives = 162/237 (68%)
Frame = +3
Query: 645 KARLLEGQLRSLSESTEREKARLNQELIQMKKEAKLSISRMSTNLERMECRAKSAEEESG 824
KA EG+L LSES EREK RL + Q+K ++KLSISR+S LE+MECRA +AE E+
Sbjct: 154 KATSFEGKLGHLSESIEREKKRLRDDHSQLKSDSKLSISRISAKLEQMECRANNAEREAE 213
Query: 825 MXXXXXXXXXXXXXXCLQQKNEVEKKLSNSMFQGVASTDATILVKHLQEELKNYESEVCE 1004
+ CL QK EVEKKLS MFQ VAST++ +LVKHLQ+EL+NYES V E
Sbjct: 214 LLKEQLKLLKDQLDECLHQKIEVEKKLSTLMFQEVASTESNVLVKHLQQELRNYESVVRE 273
Query: 1005 AKRLKSSHEDIXXXXXXXXXXXXXXXXAELDLLKLQDVHLSTTKLEDELSAWKSLLQQIP 1184
A++L+SSHE++ AE +L KL D+ L+ KLED++S+W+ ++ IP
Sbjct: 274 ARKLRSSHENVELLKEKLLEEKSHRERAESELSKLHDIQLNMKKLEDQISSWRVMITDIP 333
Query: 1185 GVSCADDVPLKFASLQKEVVNGMMKLGETAERLKKLEVALEAAEVGKQNAEADTILA 1355
GVSC +D+P+KFA+LQKEV+ K GE RLK++EVAL+AAE+GKQNAEA+ LA
Sbjct: 334 GVSCFEDLPVKFAALQKEVIYSTQKEGEITARLKQMEVALDAAEIGKQNAEAEAALA 390
Score = 148 bits (373), Expect = 4e-33
Identities = 78/126 (61%), Positives = 90/126 (71%)
Frame = +1
Query: 73 MIVRTPPLKRKAETSAPGSPNSDQRLIIFEDLPAPEPSHNPSEHMLCTYQCRQMVKSDFF 252
MIVRTPP ++ G +L+I+ED P +PSEHMLCTYQCRQMVKSDF
Sbjct: 1 MIVRTPPPPKRPRADGDG------QLVIYED----PPESSPSEHMLCTYQCRQMVKSDFI 50
Query: 253 DALNSAEKQARDSQSQLEALSDNFGKTESERKRFRDQFLYAEQELAAAKGREQALQDQLM 432
DAL+ AE QAR QS+ E L NF K ESERK+F+DQFLYA+QELAAAKGREQALQDQL+
Sbjct: 51 DALSKAENQARHYQSKFETLEPNFHKLESERKKFQDQFLYAQQELAAAKGREQALQDQLL 110
Query: 433 KEANDS 450
E S
Sbjct: 111 MEVTQS 116