BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00014527
         (1213 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002274521.1| PREDICTED: DNA-directed RNA polymerase 3, ch...   426   e-117
sp|P69242.1|RPOT3_NICSY RecName: Full=DNA-directed RNA polymeras...   424   e-116
sp|Q8L6J1.2|RPO3B_TOBAC RecName: Full=DNA-directed RNA polymeras...   421   e-115
ref|XP_002528405.1| DNA-directed RNA polymerase 3B, chloroplast ...   411   e-112
ref|XP_004162698.1| PREDICTED: DNA-directed RNA polymerase 3, ch...   397   e-108

>ref|XP_002274521.1| PREDICTED: DNA-directed RNA polymerase 3, chloroplastic [Vitis
            vinifera]
          Length = 979

 Score =  426 bits (1096), Expect = e-117
 Identities = 217/400 (54%), Positives = 271/400 (67%), Gaps = 4/400 (1%)
 Frame = +1

Query: 25   PKFIKSTQNLKHRQCVFSQSFQFKLRLNSHKHRVSVDIHPISEENSTDDLNAIAIDSMPK 204
            P F  ST +   +  + S SF+      SH        H +    + + +  +   S  +
Sbjct: 40   PHFHTSTIS---KNSLSSSSFRHNFHSPSHSSNSEAQCHALPLSPTNNSIETLQDSSNIQ 96

Query: 205  AEEANRRVFIQDPPWITSLVMRNLC----KSRQDFKLKVKEMERRKYYMLRRRQIKAETE 372
             +E+  R+F+QDPPWI+S +  + C    ++ Q+ KL+ +E ERR Y +LRRRQ+KAETE
Sbjct: 97   VKESIPRIFVQDPPWISSFLFHDWCERNRRTGQNVKLEFRETERRNYGLLRRRQVKAETE 156

Query: 373  AWXXXXXXXXXXXXXXXXKKLAPNLPYVKKLFLGWFEPLRDAIVKEQRCQKTRKHKAAYA 552
            AW                KKLAPNLPYVK LFLGWFEPLR+AI KEQR Q+T+K KAAYA
Sbjct: 157  AWERMVEEYREIEREMCEKKLAPNLPYVKALFLGWFEPLREAIDKEQRAQRTKKQKAAYA 216

Query: 553  PCIDCLPADKMAVIVMHKMMALLMTGHEGRCXXXXXXXXXXXXXXXXEVRIHLFLEKTKK 732
            P I+ LPADKMAVIVMHKMM L+M GHE  C                EVRIH FLEKTK 
Sbjct: 217  PHIELLPADKMAVIVMHKMMGLVMMGHEEGCVRVVQAAVQIGMAIEQEVRIHSFLEKTKN 276

Query: 733  HKTGKIVTQDQDDAIKETEILRKRVRSLIKKRKTTELQKIVKDEDIKSWGRDTQAKLGCR 912
             +  K V   Q+  ++E EILRKRV+S I+K+K  E+QK+VK+E+I  WGRDTQAKLG R
Sbjct: 277  FERHKTVGHSQE-GLREKEILRKRVKSFIRKKKIIEVQKLVKNEEITPWGRDTQAKLGSR 335

Query: 913  LIELLTETAYVQPPISQTVDAPPDIRPAFRHKFKIVARDPGQSFGKRYGVIECDPLVLAG 1092
            LIELLTETAYVQ P++Q+ D PPD+RPAFRH FK V ++ GQ   ++YGVIECDPLVL G
Sbjct: 336  LIELLTETAYVQTPVNQSADCPPDVRPAFRHTFKTVTKESGQKDWRKYGVIECDPLVLIG 395

Query: 1093 NEKTVKHMIIPYVPMLVPPKKWKGYDKGGYMFLPSYLMRT 1212
             ++T KHM+IPYVPML+PPKKWKGYDKGGY+FLPSY+MRT
Sbjct: 396  LDRTAKHMVIPYVPMLIPPKKWKGYDKGGYLFLPSYVMRT 435


>sp|P69242.1|RPOT3_NICSY RecName: Full=DNA-directed RNA polymerase 3, chloroplastic; AltName:
            Full=NsRpoT-C; AltName: Full=T7 bacteriophage-type single
            subunit RNA polymerase 3; Flags: Precursor
            gi|59800203|sp|P69243.1|RPO3A_TOBAC RecName:
            Full=DNA-directed RNA polymerase 3A, chloroplastic;
            AltName: Full=NictaRpoT3-syl; AltName: Full=T7
            bacteriophage-type single subunit RNA polymerase 3A;
            Flags: Precursor gi|21425641|emb|CAC82576.3| phage-type
            RNA polymerase [Nicotiana sylvestris]
            gi|21425679|emb|CAC95027.2| plastid RNA polymerase
            [Nicotiana tabacum] gi|24475541|dbj|BAC22693.1| T7
            bacteriophage-type single subunit RNA polymerase
            [Nicotiana sylvestris] gi|24475543|dbj|BAC22694.1| T7
            bacteriophage-type single subunit RNA polymerase
            [Nicotiana sylvestris]
          Length = 977

 Score =  424 bits (1090), Expect = e-116
 Identities = 218/360 (60%), Positives = 262/360 (72%), Gaps = 5/360 (1%)
 Frame = +1

Query: 148  SEENSTDDLNAIAIDSMPKAEEANRRVFIQDPPWITSLVMRNL---CKSRQDFKLKVKEM 318
            ++EN  + +N   I +    +E+N+RVFIQDPPW++SL M +L    K  Q  + + +E+
Sbjct: 75   TDENLEELVNLQKIANGVLTKESNKRVFIQDPPWVSSLFMNSLFVRAKQVQGVRREFREI 134

Query: 319  ERRKYY-MLRRRQIKAETEAWXXXXXXXXXXXXXXXXKKLAPNLPYVKKLFLGWFEPLRD 495
            ERR+ Y MLRRRQIKAETEAW                KKLAPNLPYVKKL LGWFEPLR 
Sbjct: 135  ERRRRYAMLRRRQIKAETEAWEQMVEEYRELEREMCEKKLAPNLPYVKKLLLGWFEPLRQ 194

Query: 496  AIVKEQRCQKTRKHKAAYAPCIDCLPADKMAVIVMHKMMALLMTG-HEGRCXXXXXXXXX 672
            AI KEQ  + T KH+AA+AP ID LPADKMAVIVMHK+M LLM G  E RC         
Sbjct: 195  AIEKEQNAETTVKHRAAFAPHIDSLPADKMAVIVMHKLMGLLMMGGKEERCVQVVQAAVQ 254

Query: 673  XXXXXXXEVRIHLFLEKTKKHKTGKIVTQDQDDAIKETEILRKRVRSLIKKRKTTELQKI 852
                   EVRIH FLEKTKK +      Q Q+D  +ET ILRKRV+SLIK+ +  E++K+
Sbjct: 255  IGMAVENEVRIHNFLEKTKKLQKHMTGAQSQEDMSRETMILRKRVKSLIKRNRVVEVRKL 314

Query: 853  VKDEDIKSWGRDTQAKLGCRLIELLTETAYVQPPISQTVDAPPDIRPAFRHKFKIVARDP 1032
            +K E+ +SWGRDTQAKLGCRL+ELLTETAYVQPP+ Q+ D PPDIRPAFRH F+I  RDP
Sbjct: 315  MKSEEPESWGRDTQAKLGCRLLELLTETAYVQPPVDQSADTPPDIRPAFRHVFRIATRDP 374

Query: 1033 GQSFGKRYGVIECDPLVLAGNEKTVKHMIIPYVPMLVPPKKWKGYDKGGYMFLPSYLMRT 1212
            G+S  K+YGVIECDPLV+AG ++TVK M+IPYVPMLVPPKKW+GYDKGGY+FLPSYLMRT
Sbjct: 375  GKSIVKKYGVIECDPLVVAGVDRTVKQMMIPYVPMLVPPKKWRGYDKGGYLFLPSYLMRT 434


>sp|Q8L6J1.2|RPO3B_TOBAC RecName: Full=DNA-directed RNA polymerase 3B, chloroplastic; AltName:
            Full=NictaRpoT3-tom; AltName: Full=T7 bacteriophage-type
            single subunit RNA polymerase 3B; Flags: Precursor
            gi|21425667|emb|CAC95021.2| chloroplast RNA polymerase
            [Nicotiana tabacum]
          Length = 977

 Score =  421 bits (1081), Expect = e-115
 Identities = 218/360 (60%), Positives = 261/360 (72%), Gaps = 5/360 (1%)
 Frame = +1

Query: 148  SEENSTDDLNAIAIDSMPKAEEANRRVFIQDPPWITSLVMRNL---CKSRQDFKLKVKEM 318
            ++EN  + +N   I +    +E+N+RVFIQDPPW++SL M +L    K  Q  + + +E+
Sbjct: 74   TDENLEELVNLQKIPNGFLNKESNKRVFIQDPPWVSSLFMNSLFVRAKQVQGVRREFREI 133

Query: 319  ERRKYY-MLRRRQIKAETEAWXXXXXXXXXXXXXXXXKKLAPNLPYVKKLFLGWFEPLRD 495
            ERR+ Y MLRRRQIKAETEAW                KKLAPNLPYVKKL LGWFEPLR 
Sbjct: 134  ERRRRYAMLRRRQIKAETEAWEQMVEEYRELEREMCEKKLAPNLPYVKKLLLGWFEPLRQ 193

Query: 496  AIVKEQRCQKTRKHKAAYAPCIDCLPADKMAVIVMHKMMALLMTG-HEGRCXXXXXXXXX 672
            AI KEQ  + T KH+AA+AP ID LPADKMAVIVMHK+M LLM G  E RC         
Sbjct: 194  AIEKEQNAETTVKHRAAFAPHIDSLPADKMAVIVMHKLMGLLMMGGKEERCVQVVQAAVQ 253

Query: 673  XXXXXXXEVRIHLFLEKTKKHKTGKIVTQDQDDAIKETEILRKRVRSLIKKRKTTELQKI 852
                   EVRIH FLEKTKK +      Q Q+D  KET ILRKRV+SLIK+ +  E++K+
Sbjct: 254  IGMAVENEVRIHNFLEKTKKLQKHMTGAQSQEDMSKETMILRKRVKSLIKRNRVVEVRKL 313

Query: 853  VKDEDIKSWGRDTQAKLGCRLIELLTETAYVQPPISQTVDAPPDIRPAFRHKFKIVARDP 1032
            +K E+ +SWGRDTQAKLGCRL+ELLTETAYVQ P+ Q+ D PPDIRPAFRH FKI  RDP
Sbjct: 314  MKSEEPESWGRDTQAKLGCRLLELLTETAYVQRPVDQSADTPPDIRPAFRHVFKIATRDP 373

Query: 1033 GQSFGKRYGVIECDPLVLAGNEKTVKHMIIPYVPMLVPPKKWKGYDKGGYMFLPSYLMRT 1212
            G++  K+YGVIECDPLV+AG ++TVK M+IPYVPMLVPPKKW+GYDKGGY+FLPSYLMRT
Sbjct: 374  GKNIVKKYGVIECDPLVVAGVDRTVKQMMIPYVPMLVPPKKWRGYDKGGYLFLPSYLMRT 433


>ref|XP_002528405.1| DNA-directed RNA polymerase 3B, chloroplast precursor, putative
            [Ricinus communis] gi|223532193|gb|EEF33998.1|
            DNA-directed RNA polymerase 3B, chloroplast precursor,
            putative [Ricinus communis]
          Length = 1000

 Score =  411 bits (1057), Expect = e-112
 Identities = 200/336 (59%), Positives = 252/336 (75%), Gaps = 1/336 (0%)
 Frame = +1

Query: 208  EEANRRVFIQDPPWITSLVMRNLCK-SRQDFKLKVKEMERRKYYMLRRRQIKAETEAWXX 384
            EE++RRVFIQDPPWI++L  + L K   ++ KL+ K++E+RKY +LRRRQI+ ETEAW  
Sbjct: 108  EESSRRVFIQDPPWISALFFKGLYKIPNKEVKLEFKDIEKRKYNLLRRRQIREETEAWER 167

Query: 385  XXXXXXXXXXXXXXKKLAPNLPYVKKLFLGWFEPLRDAIVKEQRCQKTRKHKAAYAPCID 564
                          +KLAPNLPYVK LFLGWFEPL++ I  EQ  Q+++K+KAA+AP I+
Sbjct: 168  MVEEYKALVKEMCERKLAPNLPYVKGLFLGWFEPLKEGIKSEQNLQRSKKNKAAFAPHIE 227

Query: 565  CLPADKMAVIVMHKMMALLMTGHEGRCXXXXXXXXXXXXXXXXEVRIHLFLEKTKKHKTG 744
             LPADKMAVIVMHKM+ LLM GHE  C                EVRIH FLEKTK ++  
Sbjct: 228  LLPADKMAVIVMHKMVGLLMVGHEDGCVPLVQAAVQIGMAIEQEVRIHNFLEKTKNYQRK 287

Query: 745  KIVTQDQDDAIKETEILRKRVRSLIKKRKTTELQKIVKDEDIKSWGRDTQAKLGCRLIEL 924
            K ++  Q+   KE EILRKRV SLI+++K  E+QK+VK+E++K WGRDTQAKLG RLIEL
Sbjct: 288  KTISGAQESLGKEKEILRKRVNSLIRRKKLIEVQKLVKNEEMKPWGRDTQAKLGSRLIEL 347

Query: 925  LTETAYVQPPISQTVDAPPDIRPAFRHKFKIVARDPGQSFGKRYGVIECDPLVLAGNEKT 1104
            LTETA+VQPP++Q+ D PPD+RPAFRH+F+IV  +PGQ   K+YGVIEC+PLVL G + +
Sbjct: 348  LTETAFVQPPVNQSADVPPDVRPAFRHRFRIVKNNPGQKIVKKYGVIECNPLVLKGLDGS 407

Query: 1105 VKHMIIPYVPMLVPPKKWKGYDKGGYMFLPSYLMRT 1212
             KHM+IPY PMLVPPKKWKGY+KGG+ FLPSY+MRT
Sbjct: 408  AKHMLIPYFPMLVPPKKWKGYNKGGHFFLPSYIMRT 443


>ref|XP_004162698.1| PREDICTED: DNA-directed RNA polymerase 3, chloroplastic-like [Cucumis
            sativus]
          Length = 975

 Score =  397 bits (1020), Expect = e-108
 Identities = 204/373 (54%), Positives = 265/373 (71%), Gaps = 11/373 (2%)
 Frame = +1

Query: 127  SVDIHPISE---ENSTDDLNAIAIDSMPKAE-------EANRRVFIQDPPWITSLVMRNL 276
            S+ + P+++   EN T+ L+      +P +E       E+++R+FIQDPPWI SL ++ L
Sbjct: 61   SLHLQPLADSVHENFTETLDNSMNLQIPISEIHQITRGESSKRIFIQDPPWIASLFLKGL 120

Query: 277  CKSRQ-DFKLKVKEMERRKYYMLRRRQIKAETEAWXXXXXXXXXXXXXXXXKKLAPNLPY 453
             K  Q + KL+  E+E+RKY +LRRRQIKAETEAW                KKLAPNLPY
Sbjct: 121  YKCVQKEVKLEFMEIEKRKYNLLRRRQIKAETEAWEKMVEEYKELEREMCEKKLAPNLPY 180

Query: 454  VKKLFLGWFEPLRDAIVKEQRCQKTRKHKAAYAPCIDCLPADKMAVIVMHKMMALLMTGH 633
            VKKLFLGWFEPL++AI +EQ+ Q+++K KAA+AP I+ L ADKMAVIVM+KMM LLM   
Sbjct: 181  VKKLFLGWFEPLKNAIEEEQKTQRSKKQKAAFAPHIELLSADKMAVIVMYKMMGLLMMAA 240

Query: 634  EGRCXXXXXXXXXXXXXXXXEVRIHLFLEKTKKHKTGKIVTQDQDDAIKETEILRKRVRS 813
            +  C                EVRI   LEK + ++  K V  D +   KE E+LRK+V  
Sbjct: 241  QDGCVQVVQAAVQIGGAIEQEVRIQSILEKARSNQRTK-VRADTECLTKEKEVLRKQVNG 299

Query: 814  LIKKRKTTELQKIVKDEDIKSWGRDTQAKLGCRLIELLTETAYVQPPISQTVDAPPDIRP 993
            LI++RK  E+QK++K E+ K+WGRDTQAKLG RLIELL E+A++QPP+SQ+ D+PPDIRP
Sbjct: 300  LIRRRKLKEVQKLLKKEEFKAWGRDTQAKLGSRLIELLIESAFIQPPVSQSADSPPDIRP 359

Query: 994  AFRHKFKIVARDPGQSFGKRYGVIECDPLVLAGNEKTVKHMIIPYVPMLVPPKKWKGYDK 1173
            AF H FK VA+ PGQ+F K+YGV+ECDP+VLAG ++T KHM+IPY+PMLVPPKKWKGYDK
Sbjct: 360  AFWHGFKPVAKTPGQNFVKKYGVVECDPIVLAGLDRTAKHMLIPYIPMLVPPKKWKGYDK 419

Query: 1174 GGYMFLPSYLMRT 1212
            GG+ FLPSY+MRT
Sbjct: 420  GGHFFLPSYVMRT 432