BLASTX nr result
ID: Bupleurum21_contig00014111
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00014111
(2312 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_003632926.1| PREDICTED: mannosyl-oligosaccharide 1,2-alph... 661 0.0
ref|NP_001238057.1| mannosyl-oligosaccharide 1,2-alpha-mannosida... 632 0.0
ref|XP_003530160.1| PREDICTED: mannosyl-oligosaccharide 1,2-alph... 633 0.0
ref|XP_004141746.1| PREDICTED: mannosyl-oligosaccharide 1,2-alph... 628 0.0
gb|AEL98979.1| mannosyl-oligosaccharide 1,2-alpha-mannosidase, p... 613 0.0
>ref|XP_003632926.1| PREDICTED: mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS1-like
[Vitis vinifera] gi|297735785|emb|CBI18472.3| unnamed
protein product [Vitis vinifera]
Length = 575
Score = 661 bits (1705), Expect(2) = 0.0
Identities = 321/408 (78%), Positives = 360/408 (88%)
Frame = -1
Query: 2009 NKWRYINPAYYLKRPKRLAFLFIAFVCISFFVWDRQTLVREHQVEVAQLNEVVLRLQNLL 1830
++WRY NP+YYLKRPKRLA LFIAFVC+SF VWDRQTLVRE ++EVA L + LQ+LL
Sbjct: 9 SRWRYCNPSYYLKRPKRLALLFIAFVCVSFLVWDRQTLVREQEIEVASLKAELTHLQDLL 68
Query: 1829 EESKTVKDALGTKSDFGGANSVTSKKNAIQDDPIDLERREKVKGAMLHAWTSYEKYAWGK 1650
+E K++ G G N +SKK+ + DDPID++RREKVK AMLHAW+SYEKYAWG+
Sbjct: 69 DELKSIG---GNAGGVSGRNGKSSKKSTVPDDPIDIQRREKVKDAMLHAWSSYEKYAWGQ 125
Query: 1649 DELQPQSKSGVDSFGGVGATLVDSLDTLYIMGLDEQFQRAREWVADSLDFNKNYDASVFE 1470
DELQPQSK+GV+SFGG+GATL+DSLDTLYIMGLDEQFQRAREWVA+SLDFNKNY+ASVFE
Sbjct: 126 DELQPQSKNGVNSFGGLGATLIDSLDTLYIMGLDEQFQRAREWVANSLDFNKNYEASVFE 185
Query: 1469 TTIRVVGGLLSAFDLSGDKIFLEKATDIANRLLPAWDTSSGIPFNFINLAHGNPHNPSWT 1290
TTIRVVGGLLSA+DLS DK+FLEKA DIA+RLLPAW+T SGIP+N INLAHGN HNP WT
Sbjct: 186 TTIRVVGGLLSAYDLSEDKLFLEKARDIADRLLPAWNTPSGIPYNIINLAHGNAHNPGWT 245
Query: 1289 AGDSILADSGTEQLEFIALSQRTGDPKYQQKAENVILQLKKIFPDDGLLPIYINPHKGTA 1110
GDSILADSGTEQLEFIALSQRTGDPKYQ+K ENVI++L K FP DGLLPIYINPH+GT+
Sbjct: 246 GGDSILADSGTEQLEFIALSQRTGDPKYQKKVENVIIELNKTFPADGLLPIYINPHRGTS 305
Query: 1109 SYATITFGAMGDSFYEYLLKVWIQGNRTAAVKHYREMWETSMTGLLSLVRRSTPSSYTYI 930
SY+TITFGAMGDSFYEYLLKVWIQGN+TAAVKHYREMWETSM GLLSLVRR+TPSS+TYI
Sbjct: 306 SYSTITFGAMGDSFYEYLLKVWIQGNKTAAVKHYREMWETSMKGLLSLVRRTTPSSFTYI 365
Query: 929 AEKMGSSLTDKMDELACFAPGMIALGSSGYDSDNSQKFLSLAEELART 786
EK G SL DKMDELACFAPGMIALGS GY + SQKFLSLAEELA T
Sbjct: 366 CEKNGDSLHDKMDELACFAPGMIALGSLGYGPEESQKFLSLAEELAWT 413
Score = 209 bits (532), Expect(2) = 0.0
Identities = 100/128 (78%), Positives = 113/128 (88%), Gaps = 3/128 (2%)
Frame = -3
Query: 696 WNILRPETLESLFYLWRLTGNKTYQDWAWNIFEAFEKNSRIESGYVGLKDVNTGVKDNMM 517
WNILRPET+ESLFYLWRLTGNKTYQ+W WNIF+AFEKNSRIESGYVGLKDVN+GVKDNMM
Sbjct: 444 WNILRPETVESLFYLWRLTGNKTYQEWGWNIFQAFEKNSRIESGYVGLKDVNSGVKDNMM 503
Query: 516 QSFFLAEAFKYLYLTFSPNSVISLDEWVFNTEAHPLRIVTRNGQGDS-EASDSQQK--LK 346
QSFFLAE KYLYL FSP++VISLDEWVFNTEAHP+RI+TR+ G++ SD QQK +
Sbjct: 504 QSFFLAETLKYLYLLFSPSTVISLDEWVFNTEAHPIRIMTRHDNGENLGGSDGQQKQFYR 563
Query: 345 VHSRKEGR 322
+ RKEGR
Sbjct: 564 LRGRKEGR 571
>ref|NP_001238057.1| mannosyl-oligosaccharide 1,2-alpha-mannosidase [Glycine max]
gi|6552504|gb|AAF16414.1|AF126550_1
mannosyl-oligosaccharide 1,2-alpha-mannosidase [Glycine
max]
Length = 578
Score = 632 bits (1631), Expect(2) = 0.0
Identities = 307/410 (74%), Positives = 355/410 (86%), Gaps = 1/410 (0%)
Frame = -1
Query: 2012 TNKWRYINPAYYLKRPKRLAFLFIAFVCISFFVWDRQTLVREHQVEVAQLNEVVLRLQNL 1833
++KWRY NP+YYLKRPKRLA LFI FVC+SF WDRQTLVREHQVE+++L + V L+NL
Sbjct: 12 SSKWRYCNPSYYLKRPKRLALLFIVFVCVSFVFWDRQTLVREHQVEISELQKEVTDLKNL 71
Query: 1832 LEESKTVKDALGTKSDFGGANSVTSKKNAIQDDPIDLERREKVKGAMLHAWTSYEKYAWG 1653
+++ + K+D G + +SK + DDPID+ERREKVK AMLHAW SYEKYAWG
Sbjct: 72 VDDLNNKQGGTSGKTDLGRKATKSSKD--VLDDPIDIERREKVKEAMLHAWGSYEKYAWG 129
Query: 1652 KDELQPQSKSGVDSFGGVGATLVDSLDTLYIMGLDEQFQRAREWVADSLDFNKNYDASVF 1473
+DELQPQSK+GV+SFGG+GATL+DSLDTLYIMGL+EQFQ+AREWVA+SLDFNK+Y+ASVF
Sbjct: 130 QDELQPQSKNGVNSFGGLGATLIDSLDTLYIMGLNEQFQKAREWVANSLDFNKDYEASVF 189
Query: 1472 ETTIRVVGGLLSAFDLSGDKIFLEKATDIANRLLPAWDTSSGIPFNFINLAHGNPHNPSW 1293
ETTIRVVGGLLSA+DLSGDK+FL+KA +IA+RLLPAW+T +GIP+N INL+HG HNPSW
Sbjct: 190 ETTIRVVGGLLSAYDLSGDKVFLDKAIEIADRLLPAWNTPTGIPYNIINLSHGRAHNPSW 249
Query: 1292 TAGDSILADSGTEQLEFIALSQRTGDPKYQQKAENVILQLKKIFPDDGLLPIYINPHKGT 1113
T G+SILADSGTEQLEFI LSQRTGD KYQQK ENVI QL K FPDDGLLPIYINPH G
Sbjct: 250 TGGESILADSGTEQLEFIVLSQRTGDLKYQQKVENVIAQLNKTFPDDGLLPIYINPHSGA 309
Query: 1112 ASYATITFGAMGDSFYEYLLKVWIQGNRTAAVKHYREMWETSMTGLLSLVRRSTPSSYTY 933
A Y+ ITFGAMGDSFYEYLLKVWIQGN+T+++KHYR+MWE SM GL SL+RRSTPSS+TY
Sbjct: 310 AGYSPITFGAMGDSFYEYLLKVWIQGNKTSSIKHYRDMWEKSMKGLSSLIRRSTPSSFTY 369
Query: 932 IAEKMGSSLTDKMDELACFAPGMIALGSSGYD-SDNSQKFLSLAEELART 786
I EK G SLTDKMDELACFAPGMIALGS GY +D+SQKFLSLAEELA T
Sbjct: 370 ICEKNGGSLTDKMDELACFAPGMIALGSFGYSAADDSQKFLSLAEELAWT 419
Score = 208 bits (529), Expect(2) = 0.0
Identities = 100/129 (77%), Positives = 112/129 (86%), Gaps = 2/129 (1%)
Frame = -3
Query: 696 WNILRPETLESLFYLWRLTGNKTYQDWAWNIFEAFEKNSRIESGYVGLKDVNTGVKDNMM 517
WNILRPET+ESLFYLWRLTGNKTYQ+W WNIF+AFEKNSRIESGYVGLKDVN+GVKDNMM
Sbjct: 450 WNILRPETVESLFYLWRLTGNKTYQEWGWNIFQAFEKNSRIESGYVGLKDVNSGVKDNMM 509
Query: 516 QSFFLAEAFKYLYLTFSPNSVISLDEWVFNTEAHPLRIVTRNGQGDSEASDSQQK--LKV 343
QSFFLAE KY YL FSP+SVISLDEWVFNTEAHPLRIVTR+ +G + + +QK ++
Sbjct: 510 QSFFLAETLKYFYLLFSPSSVISLDEWVFNTEAHPLRIVTRHEEGLVKNLNEKQKPFSRI 569
Query: 342 HSRKEGRFG 316
RKEGR G
Sbjct: 570 GGRKEGRSG 578
>ref|XP_003530160.1| PREDICTED: mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS1-like
[Glycine max]
Length = 576
Score = 633 bits (1633), Expect(2) = 0.0
Identities = 312/410 (76%), Positives = 354/410 (86%), Gaps = 1/410 (0%)
Frame = -1
Query: 2012 TNKWRYINPAYYLKRPKRLAFLFIAFVCISFFVWDRQTLVREHQVEVAQLNEVVLRLQNL 1833
++KWRY NP+YYLKRPKRLA LFI FV +SF VWDRQTLVREHQVE+++L + V LQNL
Sbjct: 11 SSKWRYCNPSYYLKRPKRLALLFIVFVGVSFIVWDRQTLVREHQVEISELRKEVTGLQNL 70
Query: 1832 LEESKTVKDALGTKSDFGGANSVTSKKNAIQDDPIDLERREKVKGAMLHAWTSYEKYAWG 1653
L++ + K+D A TSK+ + DDPID+ERREKVK AMLHAW SYEKYAWG
Sbjct: 71 LDDLNNKQGGTSGKTDLRKATK-TSKE--VLDDPIDIERREKVKEAMLHAWGSYEKYAWG 127
Query: 1652 KDELQPQSKSGVDSFGGVGATLVDSLDTLYIMGLDEQFQRAREWVADSLDFNKNYDASVF 1473
+DELQPQSK+GV+SFGG+GATL+DSLDTLYIMGL+EQFQ+AREWVA+SLDFNK+Y+ASVF
Sbjct: 128 QDELQPQSKNGVNSFGGLGATLIDSLDTLYIMGLNEQFQKAREWVANSLDFNKDYEASVF 187
Query: 1472 ETTIRVVGGLLSAFDLSGDKIFLEKATDIANRLLPAWDTSSGIPFNFINLAHGNPHNPSW 1293
ETTIRVVGGLLSA+DLSGDK+FL KA +IA+RLLPAW+T +GIP+N INL+HG HNPSW
Sbjct: 188 ETTIRVVGGLLSAYDLSGDKVFLNKAIEIADRLLPAWNTPTGIPYNIINLSHGRAHNPSW 247
Query: 1292 TAGDSILADSGTEQLEFIALSQRTGDPKYQQKAENVILQLKKIFPDDGLLPIYINPHKGT 1113
T G+SILADSGTEQLEFI LSQRTGDPKYQQK ENVI QL K FPDDGLLPIYINPH G
Sbjct: 248 TGGESILADSGTEQLEFIVLSQRTGDPKYQQKVENVIAQLNKTFPDDGLLPIYINPHSGA 307
Query: 1112 ASYATITFGAMGDSFYEYLLKVWIQGNRTAAVKHYREMWETSMTGLLSLVRRSTPSSYTY 933
A Y+ ITFGAMGDSFYEYLLKVWIQGN+T+AVKHYR+MWE SM GL SL+RRSTPSS+ Y
Sbjct: 308 AGYSPITFGAMGDSFYEYLLKVWIQGNKTSAVKHYRDMWEKSMKGLSSLIRRSTPSSFAY 367
Query: 932 IAEKMGSSLTDKMDELACFAPGMIALGSSGYDS-DNSQKFLSLAEELART 786
I EK G SLTDKMDELACFAPGMIALGS GY + D+SQKFLSLAEELA T
Sbjct: 368 ICEKNGGSLTDKMDELACFAPGMIALGSFGYSADDDSQKFLSLAEELAWT 417
Score = 206 bits (524), Expect(2) = 0.0
Identities = 99/129 (76%), Positives = 111/129 (86%), Gaps = 2/129 (1%)
Frame = -3
Query: 696 WNILRPETLESLFYLWRLTGNKTYQDWAWNIFEAFEKNSRIESGYVGLKDVNTGVKDNMM 517
WNILRPET+ESLFYLWRLTGNKTYQ+W WNIF+AFEKNSRIESGYVGLKDVN+GVKDNMM
Sbjct: 448 WNILRPETVESLFYLWRLTGNKTYQEWGWNIFQAFEKNSRIESGYVGLKDVNSGVKDNMM 507
Query: 516 QSFFLAEAFKYLYLTFSPNSVISLDEWVFNTEAHPLRIVTRNGQGDSEASDSQQK--LKV 343
QSFFLAE KY YL FSP+SVI LDEWVFNTEAHPLRIVTR+ +G + + +QK ++
Sbjct: 508 QSFFLAETLKYFYLLFSPSSVIPLDEWVFNTEAHPLRIVTRHEEGLVKNLNEKQKPFSRI 567
Query: 342 HSRKEGRFG 316
RKEGR G
Sbjct: 568 GGRKEGRSG 576
>ref|XP_004141746.1| PREDICTED: mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS2-like
[Cucumis sativus] gi|449491802|ref|XP_004159007.1|
PREDICTED: mannosyl-oligosaccharide 1,2-alpha-mannosidase
MNS2-like [Cucumis sativus]
Length = 570
Score = 628 bits (1619), Expect(2) = 0.0
Identities = 308/409 (75%), Positives = 353/409 (86%)
Frame = -1
Query: 2012 TNKWRYINPAYYLKRPKRLAFLFIAFVCISFFVWDRQTLVREHQVEVAQLNEVVLRLQNL 1833
T+KWRY +P+YYLKRPKRLAFL + FVC S VWDRQTL+REH+ E+A+L V+RL++L
Sbjct: 8 TSKWRYCHPSYYLKRPKRLAFLLVTFVCASLLVWDRQTLLREHEDELAKLKSEVVRLKSL 67
Query: 1832 LEESKTVKDALGTKSDFGGANSVTSKKNAIQDDPIDLERREKVKGAMLHAWTSYEKYAWG 1653
+EE D T + +K+ + DDPID ERREKVK A +HAW SYEKYAWG
Sbjct: 68 VEE-----DNDKTMNGIAARVHEEAKEEEV-DDPIDAERREKVKEAAIHAWNSYEKYAWG 121
Query: 1652 KDELQPQSKSGVDSFGGVGATLVDSLDTLYIMGLDEQFQRAREWVADSLDFNKNYDASVF 1473
+DELQPQSKSG++SFGG+GAT+VDSLDTLYIMGLDEQFQ+A+EWVA+SLDFNKNYDASVF
Sbjct: 122 QDELQPQSKSGINSFGGLGATIVDSLDTLYIMGLDEQFQKAKEWVANSLDFNKNYDASVF 181
Query: 1472 ETTIRVVGGLLSAFDLSGDKIFLEKATDIANRLLPAWDTSSGIPFNFINLAHGNPHNPSW 1293
ETTIRVVGGLLSA+DLSGDK+FL+KA DIA+RLLPAWDT SGIP+NFINL G PHNPSW
Sbjct: 182 ETTIRVVGGLLSAYDLSGDKLFLDKAVDIADRLLPAWDTPSGIPYNFINLVDGRPHNPSW 241
Query: 1292 TAGDSILADSGTEQLEFIALSQRTGDPKYQQKAENVILQLKKIFPDDGLLPIYINPHKGT 1113
T G SILAD+GTEQLEFI+LSQRTGDPKYQQK ENVI QL K FPDDGLLPIYI+P++G
Sbjct: 242 TGGFSILADAGTEQLEFISLSQRTGDPKYQQKVENVITQLNKNFPDDGLLPIYIDPNEGK 301
Query: 1112 ASYATITFGAMGDSFYEYLLKVWIQGNRTAAVKHYREMWETSMTGLLSLVRRSTPSSYTY 933
S+ATITFGAMGDSFYEYLLKVWI GN+T+AVKHYREMWE SM GL SLVRR+TPS++TY
Sbjct: 302 GSHATITFGAMGDSFYEYLLKVWIHGNKTSAVKHYREMWEKSMKGLSSLVRRTTPSAFTY 361
Query: 932 IAEKMGSSLTDKMDELACFAPGMIALGSSGYDSDNSQKFLSLAEELART 786
I EK GSSL+DKMDELACFAPGMIALGSSGYDS+ S+KFL+LAEELA T
Sbjct: 362 ICEKSGSSLSDKMDELACFAPGMIALGSSGYDSEESKKFLTLAEELAWT 410
Score = 202 bits (514), Expect(2) = 0.0
Identities = 97/128 (75%), Positives = 110/128 (85%), Gaps = 3/128 (2%)
Frame = -3
Query: 696 WNILRPETLESLFYLWRLTGNKTYQDWAWNIFEAFEKNSRIESGYVGLKDVNTGVKDNMM 517
WNILRPET+ESLFYLWRLTGNKTY++W WNIF+AFEKNSR+ESGYVGLKDVNTGVKDNMM
Sbjct: 441 WNILRPETVESLFYLWRLTGNKTYKEWGWNIFQAFEKNSRVESGYVGLKDVNTGVKDNMM 500
Query: 516 QSFFLAEAFKYLYLTFSPNSVISLDEWVFNTEAHPLRIVTR-NGQGDSEASDSQQK--LK 346
QSFFLAE KY+YL F+P SVI LDEWVFNTEAHPLRIVTR G+ D ++K ++
Sbjct: 501 QSFFLAETLKYIYLLFTPPSVIPLDEWVFNTEAHPLRIVTRKEGRDIERILDEKRKPFIR 560
Query: 345 VHSRKEGR 322
+ SRKEGR
Sbjct: 561 LRSRKEGR 568
>gb|AEL98979.1| mannosyl-oligosaccharide 1,2-alpha-mannosidase, partial [Silene
latifolia]
Length = 557
Score = 613 bits (1581), Expect(2) = 0.0
Identities = 305/405 (75%), Positives = 346/405 (85%)
Frame = -1
Query: 2000 RYINPAYYLKRPKRLAFLFIAFVCISFFVWDRQTLVREHQVEVAQLNEVVLRLQNLLEES 1821
RYINPAYYLKRPKRLA LFI FV + VWDRQTLVR+H+ E+A L V RLQ EE
Sbjct: 12 RYINPAYYLKRPKRLAMLFILFVVATLLVWDRQTLVRDHEEELATLLADVSRLQ---EEL 68
Query: 1820 KTVKDALGTKSDFGGANSVTSKKNAIQDDPIDLERREKVKGAMLHAWTSYEKYAWGKDEL 1641
+ +K+A G KS G+ A++DDPI++ERR+KVK AM+HAW+SYEKYAWG+DEL
Sbjct: 69 EGLKNARGMKSGDSGSK-------AVKDDPIEVERRQKVKEAMIHAWSSYEKYAWGQDEL 121
Query: 1640 QPQSKSGVDSFGGVGATLVDSLDTLYIMGLDEQFQRAREWVADSLDFNKNYDASVFETTI 1461
QPQ+K+GV+SFGG+GATLVDSLDTLYIMGLDEQFQRAR+WVA SLDFNK+YDASVFETTI
Sbjct: 122 QPQTKNGVNSFGGLGATLVDSLDTLYIMGLDEQFQRARDWVASSLDFNKDYDASVFETTI 181
Query: 1460 RVVGGLLSAFDLSGDKIFLEKATDIANRLLPAWDTSSGIPFNFINLAHGNPHNPSWTAGD 1281
RVVGGLLSA+DLS DK+FLEKA DIA+RLLPAWDT SG+P+N INLAHG PHNP WT GD
Sbjct: 182 RVVGGLLSAYDLSSDKMFLEKARDIADRLLPAWDTPSGVPYNVINLAHGRPHNPGWTGGD 241
Query: 1280 SILADSGTEQLEFIALSQRTGDPKYQQKAENVILQLKKIFPDDGLLPIYINPHKGTASYA 1101
SILADSGTEQLEFIALSQRTGDPKYQQK ENVI L K FP DGLLPIYINP G+ S +
Sbjct: 242 SILADSGTEQLEFIALSQRTGDPKYQQKVENVISVLNKTFPPDGLLPIYINPDSGSGSRS 301
Query: 1100 TITFGAMGDSFYEYLLKVWIQGNRTAAVKHYREMWETSMTGLLSLVRRSTPSSYTYIAEK 921
TITFGAMGDSFYEYLLKVWIQGN+T+AVK YR+MWETSM GL SL+RR+TPSS+TYI E+
Sbjct: 302 TITFGAMGDSFYEYLLKVWIQGNKTSAVKPYRQMWETSMKGLSSLIRRTTPSSFTYICER 361
Query: 920 MGSSLTDKMDELACFAPGMIALGSSGYDSDNSQKFLSLAEELART 786
G SLTDKMDELACFAPGM+ALGSSGY + S+K++SLAEELA T
Sbjct: 362 NGGSLTDKMDELACFAPGMLALGSSGYGPEESKKYMSLAEELAWT 406
Score = 207 bits (526), Expect(2) = 0.0
Identities = 99/124 (79%), Positives = 106/124 (85%)
Frame = -3
Query: 696 WNILRPETLESLFYLWRLTGNKTYQDWAWNIFEAFEKNSRIESGYVGLKDVNTGVKDNMM 517
WNILRPET+ESLFYLWRLTGNKTYQ+W WNIF+AFEKNSRIESGYVGLKDVNTGVKDNMM
Sbjct: 437 WNILRPETVESLFYLWRLTGNKTYQEWGWNIFQAFEKNSRIESGYVGLKDVNTGVKDNMM 496
Query: 516 QSFFLAEAFKYLYLTFSPNSVISLDEWVFNTEAHPLRIVTRNGQGDSEASDSQQKLKVHS 337
QSFFLAE KYLYL FSP SVISLDEWVFNTEAHPLRIVTR G D + ++ +
Sbjct: 497 QSFFLAETLKYLYLLFSPPSVISLDEWVFNTEAHPLRIVTRGGSSDQH---DKPVIRQRA 553
Query: 336 RKEG 325
RKEG
Sbjct: 554 RKEG 557