BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00013932
         (1888 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002279583.2| PREDICTED: uncharacterized protein LOC100265...   326   2e-86
emb|CBI16863.3| unnamed protein product [Vitis vinifera]              317   9e-84
ref|XP_002279849.1| PREDICTED: uncharacterized protein LOC100265...   288   3e-75
ref|XP_002516741.1| ubiquitin-protein ligase, putative [Ricinus ...   287   8e-75
emb|CBI16852.3| unnamed protein product [Vitis vinifera]              271   4e-70

>ref|XP_002279583.2| PREDICTED: uncharacterized protein LOC100265150 [Vitis vinifera]
          Length = 740

 Score =  326 bits (835), Expect = 2e-86
 Identities = 210/565 (37%), Positives = 311/565 (55%), Gaps = 6/565 (1%)
 Frame = -3

Query: 1868 VKNVKDTVVSPKKQSTSIKEALIASPIKKGKENKFDGIVDINLESPTPSVDVNRNIKSEE 1689
            VKNVK+ V  P+K +T  KE+++ SP K GKEN FDG                   K + 
Sbjct: 169  VKNVKEMVAYPRKPATPSKESVVVSPRKLGKENSFDG-------------------KDDP 209

Query: 1688 SGKVQNKTLENDGLAXXXXXXXXXXXXXXXXXSNLYADLEDVATLKGKSLHVNKKAKKNS 1509
            +  +++ TL  D                                         KK KK +
Sbjct: 210  NLHLEDLTLSPD----------------------------------------EKKPKKTT 229

Query: 1508 SREDLSKLHNGGIGSGALLENRPKKPPISDVSNHNEAKPDGKGDSVLV--AGDDCNAKAS 1335
            S E     HN   GS  L ++  K   I++  ++NEAKP+ +   +L   + +  +++  
Sbjct: 230  SEELKEHDHNVDNGS-CLKKSNLKGLCITNELSNNEAKPEQEDGDILQEKSSETGSSQKV 288

Query: 1334 IEVCVESLKNKKRNA----NKDIEEPALKKSKNGVKQDTVKNKRNGLLSESKDLGVDTPM 1167
                +E  +N++ +     N    + A  K    VK  TVKN    L  +++   VD P+
Sbjct: 289  SSDSIEIGENREGDGTICQNMGGLDNAGAKRYKVVKSHTVKNT---LKFQNRAFVVDVPL 345

Query: 1166 PIGIELSSIDGVELPLEEDVGNALQFLEFCAAFGKTLNMKKGQSGIILQELLSGPTRVLR 987
            P G +L+++ G+ELP  EDVG+ALQFLEFCAAFGK  +++KGQS  I+QEL+SG ++   
Sbjct: 346  PQGTKLTTVTGIELP-PEDVGDALQFLEFCAAFGKAFDLRKGQSESIMQELISGCSK--- 401

Query: 986  RSQQQSAAIQFHIXXXXXXXXXXXQRPQTLSPSHGKNSWLLALKKCISESPNAVDSLPID 807
               QQS  ++FHI           Q  + LS ++GKNSWL+AL +C+S+S  A   LP D
Sbjct: 402  HRGQQSLGVRFHIKLLSLILKDLGQDSRPLSSANGKNSWLVALGQCVSKSQCASKELPSD 461

Query: 806  NLDGESGGYNTLDFSTKLRILVFLCDEVLETAKIRDWIHNQXXXXXXXXXXXXERFPGAK 627
              D  S GY+ LDFS K+R+L FLCDEVL T K+R W   Q            E+   AK
Sbjct: 462  CFDRGSDGYDELDFSKKIRLLNFLCDEVLCTEKLRSWTDRQNSKFVERVKEAKEKVLAAK 521

Query: 626  EKEKSLKQNIMNEVAKTIVAKDGDALSVAEHEEIIRQIKAEAAQAHREKLESLNLIPKTK 447
            +K+K +KQ + +E AK I+A++   + ++EHE  + +IK E AQAH E LE+++++PK K
Sbjct: 522  DKKKLMKQKLQDETAKAIIAQNSAPILISEHEPTVSKIKTEVAQAHAEMLEAISMVPKRK 581

Query: 446  ERAAAVRTEPIFVDVDGRAFWRLNGYFNKSYILVQDIGNGDDTIPLGEKWFAFDVEQEKM 267
            +R+ AVRTEP+ +DV+G +FWRLNGY  +  IL+QD+G  D+ I   EKWF++++ Q+K 
Sbjct: 582  QRSDAVRTEPLLLDVNGHSFWRLNGYSGEPDILLQDMGTTDEGIH-DEKWFSYELGQKKS 640

Query: 266  VEKHISFRDKMLRAQNVAEIAPSQT 192
            VE++ISFR+K LR   V E  P ++
Sbjct: 641  VEEYISFREKKLRLHTVTEKLPLES 665


>emb|CBI16863.3| unnamed protein product [Vitis vinifera]
          Length = 659

 Score =  317 bits (811), Expect = 9e-84
 Identities = 206/559 (36%), Positives = 294/559 (52%)
 Frame = -3

Query: 1868 VKNVKDTVVSPKKQSTSIKEALIASPIKKGKENKFDGIVDINLESPTPSVDVNRNIKSEE 1689
            VKNVK+ V  P+K +T  KE+++ SP K GKEN FDG                   K + 
Sbjct: 169  VKNVKEMVAYPRKPATPSKESVVVSPRKLGKENSFDG-------------------KDDP 209

Query: 1688 SGKVQNKTLENDGLAXXXXXXXXXXXXXXXXXSNLYADLEDVATLKGKSLHVNKKAKKNS 1509
            +  +++ TL  D                                         KK KK +
Sbjct: 210  NLHLEDLTLSPD----------------------------------------EKKPKKTT 229

Query: 1508 SREDLSKLHNGGIGSGALLENRPKKPPISDVSNHNEAKPDGKGDSVLVAGDDCNAKASIE 1329
            S E   K H+  + +G+ L+    K  +S  S       +G G      G   NA A   
Sbjct: 230  SEE--LKEHDHNVDNGSCLKKSNLKGLLSSDSIEIGENREGDGTICQNMGGLDNAGA--- 284

Query: 1328 VCVESLKNKKRNANKDIEEPALKKSKNGVKQDTVKNKRNGLLSESKDLGVDTPMPIGIEL 1149
                                   K    VK  TVKN    L  +++   VD P+P G +L
Sbjct: 285  -----------------------KRYKVVKSHTVKNT---LKFQNRAFVVDVPLPQGTKL 318

Query: 1148 SSIDGVELPLEEDVGNALQFLEFCAAFGKTLNMKKGQSGIILQELLSGPTRVLRRSQQQS 969
            +++ G+ELP  EDVG+ALQFLEFCAAFGK  +++KGQS  I+QEL+SG ++      QQS
Sbjct: 319  TTVTGIELP-PEDVGDALQFLEFCAAFGKAFDLRKGQSESIMQELISGCSK---HRGQQS 374

Query: 968  AAIQFHIXXXXXXXXXXXQRPQTLSPSHGKNSWLLALKKCISESPNAVDSLPIDNLDGES 789
              ++FHI           Q  + LS ++GKNSWL+AL +C+S+S  A   LP D  D  S
Sbjct: 375  LGVRFHIKLLSLILKDLGQDSRPLSSANGKNSWLVALGQCVSKSQCASKELPSDCFDRGS 434

Query: 788  GGYNTLDFSTKLRILVFLCDEVLETAKIRDWIHNQXXXXXXXXXXXXERFPGAKEKEKSL 609
             GY+ LDFS K+R+L FLCDEVL T K+R W   Q            E+   AK+K+K +
Sbjct: 435  DGYDELDFSKKIRLLNFLCDEVLCTEKLRSWTDRQNSKFVERVKEAKEKVLAAKDKKKLM 494

Query: 608  KQNIMNEVAKTIVAKDGDALSVAEHEEIIRQIKAEAAQAHREKLESLNLIPKTKERAAAV 429
            KQ + +E AK I+A++   + ++EHE  + +IK E AQAH E LE+++++PK K+R+ AV
Sbjct: 495  KQKLQDETAKAIIAQNSAPILISEHEPTVSKIKTEVAQAHAEMLEAISMVPKRKQRSDAV 554

Query: 428  RTEPIFVDVDGRAFWRLNGYFNKSYILVQDIGNGDDTIPLGEKWFAFDVEQEKMVEKHIS 249
            RTEP+ +DV+G +FWRLNGY  +  IL+QD+G  D+ I   EKWF++++ Q+K VE++IS
Sbjct: 555  RTEPLLLDVNGHSFWRLNGYSGEPDILLQDMGTTDEGIH-DEKWFSYELGQKKSVEEYIS 613

Query: 248  FRDKMLRAQNVAEIAPSQT 192
            FR+K LR   V E  P ++
Sbjct: 614  FREKKLRLHTVTEKLPLES 632


>ref|XP_002279849.1| PREDICTED: uncharacterized protein LOC100265236 [Vitis vinifera]
          Length = 805

 Score =  288 bits (737), Expect = 3e-75
 Identities = 218/636 (34%), Positives = 316/636 (49%), Gaps = 75/636 (11%)
 Frame = -3

Query: 1874 GLVKNVKDTVVSPKKQSTSIKEALIASPIKKGKENKFDGIVDINLESP----TPSVDVNR 1707
            G  KNVKD V SP K+S      LI SP K+GKEN FDG  D NL       +P     +
Sbjct: 165  GSCKNVKDIVCSPSKES------LIVSPRKRGKENSFDGKKDSNLHPLKLMLSPDETKPK 218

Query: 1706 NIKSEESGKVQNKTLENDGLAXXXXXXXXXXXXXXXXXSNLYAD--LEDVATLKGKSLHV 1533
             IK E+         E+DG                   + + AD   +DV   KG    V
Sbjct: 219  KIKREQ-------LKEHDG-NKDGGASLKKSSSRRPSKNEVKADGKEDDVLQEKGCGASV 270

Query: 1532 NKKAKKNSS-----REDLSKLHNGGIGSGALLENRPKKP--------------------- 1431
            ++K   +S+      E  +K +  G+   A+++N   KP                     
Sbjct: 271  SQKVSLDSNVIGKKGEQGAKPNKVGVLGNAIIDNVGAKPNKVVRSHKVKKHRMKLRRKEF 330

Query: 1430 ----PISDVSNHNEAKPD----------GKGDSVLVAGDDCNAKA-----SIEVCVESLK 1308
                P+S  SN    K +          G  DS ++  D+  AK      S +V    +K
Sbjct: 331  DVDVPVSHDSNVTGEKGEEGDCTTSQNMGVLDSAII--DNVGAKPNEVVRSHKVKKHRMK 388

Query: 1307 NKKRNANKDI-------------EEPALKKSKN-GVKQDTV----------KNKRNGLLS 1200
             +K+  + D+             E+   K  +N GV  + +          K +++ +  
Sbjct: 389  FQKKEFDVDVSMSCDSNVIGDKGEDGDCKTCQNVGVLDNAIIDNAGAKPNNKVRKHSMKF 448

Query: 1199 ESKDLGVDTPMPIGIELSSIDGVELPLEEDVGNALQFLEFCAAFGKTLNMKKGQSGIILQ 1020
            + K+  VD P+P GI L++I G+EL   EDVG+ALQFLEFCAAFGK   ++KGQS  +LQ
Sbjct: 449  QLKEFDVDVPLPQGISLTTIAGIELS-PEDVGHALQFLEFCAAFGKVFGLRKGQSESVLQ 507

Query: 1019 ELLSGPTRVLRRSQQQSAAIQFHIXXXXXXXXXXXQRPQTLSPSHGKNSWLLALKKCISE 840
            EL+ G      R    S   Q HI           Q  Q L+ + G NSWL AL KCIS 
Sbjct: 508  ELVHGYNGPQGR---HSLTAQLHIKLLSLILKDLGQHSQPLTSTKGNNSWLKALGKCIST 564

Query: 839  SPNAVDSLPIDNLDGESGGYNTLDFSTKLRILVFLCDEVLETAKIRDWIHNQXXXXXXXX 660
            SP A+  LP+D  +  S GY+TLDFS +LR+L FLCDE L T ++R +I  Q        
Sbjct: 565  SPCALKELPLDCFESGSDGYDTLDFSKRLRLLNFLCDESLCTERLRRYIDTQNSKFVEKE 624

Query: 659  XXXXERFPGAKEKEKSLKQNIMNEVAKTIVAKDGDALSVAEHEEIIRQIKAEAAQAHREK 480
                E+   AK+K K +KQ + +E  K I+A++   LS+++HE +  QIK E  QAH E 
Sbjct: 625  KAAKEKVLAAKDKTKRMKQKLQDERVKAIIAQNSTPLSISKHETVFSQIKTEVEQAHSEM 684

Query: 479  LESLNLIPKTKERAAAVRTEPIFVDVDGRAFWRLNGYFNKSYILVQDIGNGDDTIPLGEK 300
            LE++N++ K K+R+ AVRTE I +DV+G +FWRL  Y  +  IL++D+G      P  EK
Sbjct: 685  LEAINMLSKMKQRSDAVRTESILLDVNGHSFWRLKVYAGEPDILLRDMGTWIADAP-DEK 743

Query: 299  WFAFDVEQEKMVEKHISFRDKMLRAQNVAEIAPSQT 192
            WF++D+EQ+  +E++IS R+K LR   + ++ P ++
Sbjct: 744  WFSYDLEQKDSIEEYISLREKKLRFHKIPKMRPIES 779


>ref|XP_002516741.1| ubiquitin-protein ligase, putative [Ricinus communis]
            gi|223544114|gb|EEF45639.1| ubiquitin-protein ligase,
            putative [Ricinus communis]
          Length = 687

 Score =  287 bits (734), Expect = 8e-75
 Identities = 207/590 (35%), Positives = 303/590 (51%), Gaps = 23/590 (3%)
 Frame = -3

Query: 1886 PENFGLVKNVKDTVVSPKKQSTSIKEALIASPIKKGKENKFDGIVDINLESPTPSVDVNR 1707
            PENF   +   +T  S  K + S KE++IASP K GKEN  DG  D +    +P      
Sbjct: 148  PENFACDRFPNNTGASLNKPA-SAKESIIASPRKLGKENSLDGKCDSHSPKSSPV----- 201

Query: 1706 NIKSEESGKVQNKTLENDGLAXXXXXXXXXXXXXXXXXSNLYADLEDVATLKGKSLHVNK 1527
                  S K ++K  +++GL                          DV +L+  S    +
Sbjct: 202  ------SNKKKHKKAKSEGL-------------------------HDVNSLRDSS----R 226

Query: 1526 KAKKNSSREDLSKLHNGGIGSGALLENRPKKPPISDVSNHNEAKPDGKGDSVLVAGDDCN 1347
            K  + +     +K+   G    +L++    K    D+    E K + K + V+  G    
Sbjct: 227  KKPRFTEEVLTNKMKINGKDEDSLVKTCKSKIEFKDIPK-KEVKKNDKVEGVIAVGKKFK 285

Query: 1346 AKASIEVCVESLKNKKRNANKDIEEPALKKSKNGV------KQDTVKNKRNGLLS----- 1200
             K S +V  + + N K  +  +  +P +  S+N V      K+DT   K  G+       
Sbjct: 286  -KQSQDVSKKEVINVKAESENENFQPQI--SRNVVCLIANEKRDTGNCKSIGVPGGVKCN 342

Query: 1199 --------ESKDLGVDTPMPIGIELSSIDGVELPLEEDVGNALQFLEFCAAFGKTLNMKK 1044
                    +SK    D  +P G  L+++ G+ELP  ED G+ALQF EFCAAF + L+++K
Sbjct: 343  VDKGTIELQSKQTEDDIQLPSGTCLTNVAGIELP-HEDAGHALQFFEFCAAFAEVLDLRK 401

Query: 1043 GQSGIILQELLSGPTRVLRRSQQQSAAIQFHIXXXXXXXXXXXQRPQTLSPSHGKNSWLL 864
            GQ+  +++E++ G  R  RRS   S  +QF I           +   TLS ++G+ SWL 
Sbjct: 402  GQAEAVIREIIFG--RKARRSHS-SLLVQFQIKLLSLILEDMGEESPTLSTANGQTSWLK 458

Query: 863  ALKKCISESPNAVDSLPIDNLDGESGGYNTLDFSTKLRILVFLCDEVLETAKIRDWIHNQ 684
            A  KC+S+        P D  D  +  Y+ L+ S K ++L FLCDE L T  +R WI ++
Sbjct: 459  AFGKCVSDRKFMSKEFPSDCFDRGNERYDMLNTSEKFKLLNFLCDEALNTKDLRSWIDDR 518

Query: 683  XXXXXXXXXXXXERFPGAKEKEKSLKQNIMNEVAKTIVAKDGDALSVAEHEEIIRQIKAE 504
                        E+   AK+KEK+LKQ + +EVAK I+AK G   SV+EHE I+ QIK E
Sbjct: 519  NSKFVEKEKEAKEKVLAAKDKEKNLKQKVHDEVAKAIIAKSGAPFSVSEHEAIVSQIKKE 578

Query: 503  AAQAHREKLESLNLIPKTKERAAAVRTEPIFVDVDGRAFWRLNGYFNKSYILVQDIGNGD 324
            AAQAH E + ++ ++PK ++R+ AVRT+PI +DV+G AFWRL GY  +S IL+QD+GN  
Sbjct: 579  AAQAHVEMMAAVGMVPKKRQRSDAVRTDPILLDVNGHAFWRLKGYNGQSDILLQDMGNWT 638

Query: 323  DTIPLGEKWFAFDVEQEKMVEKHI-SFRDKMLRAQNVA---EIAPSQTNI 186
               P  EKWF +D EQ++ VEK+I S R K LRAQ VA     A S+TN+
Sbjct: 639  VAAP-EEKWFVYDAEQKQGVEKYISSLRTKRLRAQKVAGRPSYADSETNL 687


>emb|CBI16852.3| unnamed protein product [Vitis vinifera]
          Length = 574

 Score =  271 bits (693), Expect = 4e-70
 Identities = 164/429 (38%), Positives = 236/429 (55%), Gaps = 1/429 (0%)
 Frame = -3

Query: 1475 GIGSGALLENRPKKPPISDVSNHNEA-KPDGKGDSVLVAGDDCNAKASIEVCVESLKNKK 1299
            G     +L    K    S VS   +A  PDG           C     I      ++  K
Sbjct: 135  GCNPTGMLVYTAKATGFSSVSEMLQAIDPDGS----------CKNVKDIVCSPSKVRPSK 184

Query: 1298 RNANKDIEEPALKKSKNGVKQDTVKNKRNGLLSESKDLGVDTPMPIGIELSSIDGVELPL 1119
                 D +E  + + K      + K +++ +  + K+  VD P+P GI L++I G+EL  
Sbjct: 185  NEVKADGKEDDVLQEKGCGASVSQKVRKHSMKFQLKEFDVDVPLPQGISLTTIAGIELS- 243

Query: 1118 EEDVGNALQFLEFCAAFGKTLNMKKGQSGIILQELLSGPTRVLRRSQQQSAAIQFHIXXX 939
             EDVG+ALQFLEFCAAFGK   ++KGQS  +LQEL+ G      R    S   Q HI   
Sbjct: 244  PEDVGHALQFLEFCAAFGKVFGLRKGQSESVLQELVHGYNGPQGR---HSLTAQLHIKLL 300

Query: 938  XXXXXXXXQRPQTLSPSHGKNSWLLALKKCISESPNAVDSLPIDNLDGESGGYNTLDFST 759
                    Q  Q L+ + G NSWL AL KCIS SP A+  LP+D  +  S GY+TLDFS 
Sbjct: 301  SLILKDLGQHSQPLTSTKGNNSWLKALGKCISTSPCALKELPLDCFESGSDGYDTLDFSK 360

Query: 758  KLRILVFLCDEVLETAKIRDWIHNQXXXXXXXXXXXXERFPGAKEKEKSLKQNIMNEVAK 579
            +LR+L FLCDE L T ++R +I  Q            E+   AK+K K +KQ + +E  K
Sbjct: 361  RLRLLNFLCDESLCTERLRRYIDTQNSKFVEKEKAAKEKVLAAKDKTKRMKQKLQDERVK 420

Query: 578  TIVAKDGDALSVAEHEEIIRQIKAEAAQAHREKLESLNLIPKTKERAAAVRTEPIFVDVD 399
             I+A++   LS+++HE +  QIK E  QAH E LE++N++ K K+R+ AVRTE I +DV+
Sbjct: 421  AIIAQNSTPLSISKHETVFSQIKTEVEQAHSEMLEAINMLSKMKQRSDAVRTESILLDVN 480

Query: 398  GRAFWRLNGYFNKSYILVQDIGNGDDTIPLGEKWFAFDVEQEKMVEKHISFRDKMLRAQN 219
            G +FWRL  Y  +  IL++D+G      P  EKWF++D+EQ+  +E++IS R+K LR   
Sbjct: 481  GHSFWRLKVYAGEPDILLRDMGTWIADAP-DEKWFSYDLEQKDSIEEYISLREKKLRFHK 539

Query: 218  VAEIAPSQT 192
            + ++ P ++
Sbjct: 540  IPKMRPIES 548