BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00013831
         (1742 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002273194.2| PREDICTED: O-glucosyltransferase rumi-like [...   576   e-162
emb|CAN70600.1| hypothetical protein VITISV_027960 [Vitis vinifera]   575   e-161
ref|XP_002273411.2| PREDICTED: O-glucosyltransferase rumi-like [...   563   e-158
ref|XP_002273475.2| PREDICTED: O-glucosyltransferase rumi [Vitis...   560   e-157
ref|XP_002273350.2| PREDICTED: O-glucosyltransferase rumi homolo...   558   e-156

>ref|XP_002273194.2| PREDICTED: O-glucosyltransferase rumi-like [Vitis vinifera]
          Length = 521

 Score =  576 bits (1484), Expect = e-162
 Identities = 270/479 (56%), Positives = 341/479 (71%), Gaps = 4/479 (0%)
 Frame = -2

Query: 1522 KIGAFTSVTLFCICL-FIGALVATQMLDVPIFTGAPLQKSSPTTKIH--KFTYQLQC-SG 1355
            K GA  +V+LF I L FIGALV+T+ ++V I TG  L+ +SPT K+H  KF Y L C  G
Sbjct: 31   KKGATVTVSLFTIILIFIGALVSTRCINVSILTGDFLKNTSPT-KLHSQKFEYSLNCREG 89

Query: 1354 NTTQTCPXXXXXXXXXXXTSASQCPEYFRWIYEDLKPWEKTGLTKEIVESGMHKANLRII 1175
            N +QTCP             +  CPEYFRWIYEDL+PW  TG+T+ +VE     A++RI+
Sbjct: 90   NMSQTCPVTGPVAFEPGEPPSETCPEYFRWIYEDLRPWMDTGITRAMVEKARPAASIRIV 149

Query: 1174 IVDGKLYTQKYSGVFQTRDVFTIWGIVQLLKLYPGKVPDVDFMFECGDMPMIHKRDYMGA 995
            +VDGK+Y +KY  V + RD FTIWGI+QLL++YPGK+PD D MFEC D PMI    Y G 
Sbjct: 150  VVDGKVYVEKYKRVNRNRDEFTIWGILQLLRMYPGKLPDFDLMFECRDRPMIRTHLYQGP 209

Query: 994  NASIPPPLFHYCGDDSTLDLVFPDWSFWGWPELNIRPWVSLXXXXXXXXXXXXXXKREPY 815
            +A++PPPLFHYCGDD T D+VFPDWSFWGWPE NI+PW                  REPY
Sbjct: 210  DATVPPPLFHYCGDDETYDIVFPDWSFWGWPETNIKPWNGFKKDLKEGNYRTKWIDREPY 269

Query: 814  AYWKGNAWVSNNRGDLMKCNKSDKNDWKARLYQVNWVDESKKGFNRTNLADQCTHRYKIY 635
            AYWKGN  +   R +L KC  +D+ DW ARLY ++W  E + GF  ++LA QCTHRYKIY
Sbjct: 270  AYWKGNVKMGVVRKELFKCRNTDEQDWNARLYIMDWGREVQSGFKTSDLASQCTHRYKIY 329

Query: 634  IEGRAWSVSEKYILACDSMTLLVKPQYYDFFTRSLQPLVHYWPVNKNNKCRSVKFAVDWG 455
             EG AWSVSEKYILACDS+TLLVKPQYY+FFTRSLQPLVHYWP+   + C+S+KFA DW 
Sbjct: 330  TEGIAWSVSEKYILACDSVTLLVKPQYYEFFTRSLQPLVHYWPIKHKDMCKSIKFATDWC 389

Query: 454  NSHPKKAQRIGRAGSKFIQEGLNMKSVYDYMYHTLNEYAKLMKYKPTVPPQATQVCSETM 275
            N+H +KAQ+IG+AGS F+QE + MK VYDYM+H L+ YAKL+KYKPTVPP A + C E M
Sbjct: 390  NNHTEKAQKIGKAGSSFVQEEIKMKFVYDYMFHLLSMYAKLLKYKPTVPPMAVEFCPEMM 449

Query: 274  ACSEHQELNKRFKIESMVMNPSDTNPCILAPSYSNNEVHAIRDKQESTRKQVELWENNG 98
            AC+  + L K +K++SMV +PSDT PCI+ P +S+ E+  + +K++   KQVE WE +G
Sbjct: 450  ACAV-EGLEKDYKLQSMVKSPSDTGPCIMPPPFSSAELKDVLEKKDHVMKQVETWEESG 507


>emb|CAN70600.1| hypothetical protein VITISV_027960 [Vitis vinifera]
          Length = 521

 Score =  575 bits (1483), Expect = e-161
 Identities = 270/479 (56%), Positives = 341/479 (71%), Gaps = 4/479 (0%)
 Frame = -2

Query: 1522 KIGAFTSVTLFCICL-FIGALVATQMLDVPIFTGAPLQKSSPTTKIH--KFTYQLQC-SG 1355
            K GA  +V+LF I L FIGALV+T+ ++V I TG  L+ +SPT K+H  KF Y L C  G
Sbjct: 31   KKGATVTVSLFTIILIFIGALVSTRCINVSILTGDFLKNTSPT-KLHSQKFEYSLNCREG 89

Query: 1354 NTTQTCPXXXXXXXXXXXTSASQCPEYFRWIYEDLKPWEKTGLTKEIVESGMHKANLRII 1175
            N +QTCP             +  CPEYFRWIYEDL+PW  TG+T+ +VE     A++RI+
Sbjct: 90   NMSQTCPVTGPVAFEPGEPPSETCPEYFRWIYEDLRPWMDTGITRAMVEKARPAASIRIV 149

Query: 1174 IVDGKLYTQKYSGVFQTRDVFTIWGIVQLLKLYPGKVPDVDFMFECGDMPMIHKRDYMGA 995
            +VDGK+Y +KY  V + RD FTIWGI+QLL++YPGK+PD D MFEC D PMI    Y G 
Sbjct: 150  VVDGKVYVEKYKRVNRNRDEFTIWGILQLLRMYPGKLPDFDLMFECRDRPMIKTHLYQGP 209

Query: 994  NASIPPPLFHYCGDDSTLDLVFPDWSFWGWPELNIRPWVSLXXXXXXXXXXXXXXKREPY 815
            +A++PPPLFHYCGDD T D+VFPDWSFWGWPE NI+PW                  REPY
Sbjct: 210  DATVPPPLFHYCGDDETYDIVFPDWSFWGWPETNIKPWNGFKKDLKEGNYRTKWIDREPY 269

Query: 814  AYWKGNAWVSNNRGDLMKCNKSDKNDWKARLYQVNWVDESKKGFNRTNLADQCTHRYKIY 635
            AYWKGN  +   R +L KC  +D+ DW ARLY ++W  E + GF  ++LA QCTHRYKIY
Sbjct: 270  AYWKGNVKMGVVRKELFKCRNTDEQDWNARLYIMDWGREVQSGFKTSDLASQCTHRYKIY 329

Query: 634  IEGRAWSVSEKYILACDSMTLLVKPQYYDFFTRSLQPLVHYWPVNKNNKCRSVKFAVDWG 455
             EG AWSVSEKYILACDS+TLLVKPQYY+FFTRSLQPLVHYWP+   + C+S+KFA DW 
Sbjct: 330  TEGIAWSVSEKYILACDSVTLLVKPQYYEFFTRSLQPLVHYWPIKHKDMCKSIKFATDWC 389

Query: 454  NSHPKKAQRIGRAGSKFIQEGLNMKSVYDYMYHTLNEYAKLMKYKPTVPPQATQVCSETM 275
            N+H +KAQ+IG+AGS F+QE + MK VYDYM+H L+ YAKL+KYKPTVPP A + C E M
Sbjct: 390  NNHTEKAQKIGKAGSSFVQEEIKMKFVYDYMFHLLSMYAKLLKYKPTVPPMAVEFCPEMM 449

Query: 274  ACSEHQELNKRFKIESMVMNPSDTNPCILAPSYSNNEVHAIRDKQESTRKQVELWENNG 98
            AC+  + L K +K++SMV +PSDT PCI+ P +S+ E+  + +K++   KQVE WE +G
Sbjct: 450  ACAV-EGLEKDYKLQSMVKSPSDTGPCIMPPPFSSAELKDVLEKKDHVMKQVETWEESG 507


>ref|XP_002273411.2| PREDICTED: O-glucosyltransferase rumi-like [Vitis vinifera]
          Length = 525

 Score =  563 bits (1451), Expect = e-158
 Identities = 266/482 (55%), Positives = 337/482 (69%), Gaps = 7/482 (1%)
 Frame = -2

Query: 1522 KIGAFTSVTLFCICL-FIGALVATQMLDVPIFTGAPLQKS---SPTTKIH--KFTYQLQC 1361
            K GA  +V+LF I L FIGALV+T+ ++V I TG  L+K+   +  TK H  KF Y L C
Sbjct: 31   KKGATVTVSLFTIILIFIGALVSTRWINVSILTGDFLKKTLNYTSPTKPHSQKFEYSLNC 90

Query: 1360 -SGNTTQTCPXXXXXXXXXXXTSASQCPEYFRWIYEDLKPWEKTGLTKEIVESGMHKANL 1184
              GN +QTCP             +  CPEYFRWIYEDL+PW  TG+T+ +VE     A++
Sbjct: 91   REGNVSQTCPVTGPVAFEPSEPPSETCPEYFRWIYEDLRPWMDTGITRAMVEKARPAASI 150

Query: 1183 RIIIVDGKLYTQKYSGVFQTRDVFTIWGIVQLLKLYPGKVPDVDFMFECGDMPMIHKRDY 1004
            RI++VDGK+Y +KY  V   RD FTIWGI+QLL++YP K+PD D MFEC D PMI    Y
Sbjct: 151  RIVVVDGKVYMEKYKRVNHNRDEFTIWGILQLLRMYPEKLPDFDLMFECRDRPMIKTHLY 210

Query: 1003 MGANASIPPPLFHYCGDDSTLDLVFPDWSFWGWPELNIRPWVSLXXXXXXXXXXXXXXKR 824
             G +A++PPPLFHYCGDD T D+VFPDWSFWGWPE NI+PW                  R
Sbjct: 211  QGPDATVPPPLFHYCGDDETYDIVFPDWSFWGWPETNIKPWNGFKKDLKEGNYRTKWIDR 270

Query: 823  EPYAYWKGNAWVSNNRGDLMKCNKSDKNDWKARLYQVNWVDESKKGFNRTNLADQCTHRY 644
            EPYAYWKGN  +   R +L KC  +D+ DW ARLY ++W  E + GF  ++LA QCTHRY
Sbjct: 271  EPYAYWKGNVKMGVVRKELFKCRNTDEQDWNARLYIMDWGREVQSGFKTSDLASQCTHRY 330

Query: 643  KIYIEGRAWSVSEKYILACDSMTLLVKPQYYDFFTRSLQPLVHYWPVNKNNKCRSVKFAV 464
            KIY EG  WSVSEKYILACDS+TLLVKPQYY+FFTRSLQPLVHYWP+   + C+S+KFA 
Sbjct: 331  KIYTEGIGWSVSEKYILACDSVTLLVKPQYYEFFTRSLQPLVHYWPIKHKDMCKSIKFAT 390

Query: 463  DWGNSHPKKAQRIGRAGSKFIQEGLNMKSVYDYMYHTLNEYAKLMKYKPTVPPQATQVCS 284
            DW N+H +KAQ+IG+AGS F+QE + MK VYDYM+H L+ YAKL+KYKPTVPP A + C 
Sbjct: 391  DWCNNHTEKAQKIGKAGSGFVQEEIKMKFVYDYMFHLLSMYAKLLKYKPTVPPMAVEFCP 450

Query: 283  ETMACSEHQELNKRFKIESMVMNPSDTNPCILAPSYSNNEVHAIRDKQESTRKQVELWEN 104
            E MAC+  + L K +K++SMV +PSDT PCI+ P +++ E+  + +K++   KQVE WE 
Sbjct: 451  EMMACAV-EGLEKDYKLQSMVKSPSDTGPCIMPPPFNSAELKDVLEKKDHVMKQVETWEE 509

Query: 103  NG 98
            +G
Sbjct: 510  SG 511


>ref|XP_002273475.2| PREDICTED: O-glucosyltransferase rumi [Vitis vinifera]
          Length = 604

 Score =  560 bits (1444), Expect = e-157
 Identities = 259/479 (54%), Positives = 329/479 (68%), Gaps = 6/479 (1%)
 Frame = -2

Query: 1516 GAFTSVTLFCICLFIGALVATQMLDVPIFTGAPLQK-----SSPTTKIHKFTYQLQCS-G 1355
            GA  +V+LF I LFIGA ++T+  +V +FTG   +K     SS      K  Y L CS G
Sbjct: 121  GATMTVSLFSIILFIGAFISTRWSNVSVFTGDSFRKVWSHSSSIKPCNKKIEYSLNCSEG 180

Query: 1354 NTTQTCPXXXXXXXXXXXTSASQCPEYFRWIYEDLKPWEKTGLTKEIVESGMHKANLRII 1175
            N  QTCP            S   CPEYFRWIYEDL+PW +TG+T+++VE     A +R++
Sbjct: 181  NMAQTCPVTPLATFEPSEPSTETCPEYFRWIYEDLRPWRETGITRDMVERAKPAAYIRVV 240

Query: 1174 IVDGKLYTQKYSGVFQTRDVFTIWGIVQLLKLYPGKVPDVDFMFECGDMPMIHKRDYMGA 995
            +VDGK+Y +KY GV+QTRDVFTIWGI+QLL++YPGK+PD D MF CGD   +  R + G 
Sbjct: 241  VVDGKVYMEKYKGVYQTRDVFTIWGILQLLRMYPGKLPDFDLMFSCGDKLAMKTRYFQGP 300

Query: 994  NASIPPPLFHYCGDDSTLDLVFPDWSFWGWPELNIRPWVSLXXXXXXXXXXXXXXKREPY 815
            NA+ PPPLFHYCGDD T D+VFPDWSFWGWPE++I+ W +L               REPY
Sbjct: 301  NATTPPPLFHYCGDDETYDIVFPDWSFWGWPEIHIKQWNTLKKDLKEGNNRTEWIDREPY 360

Query: 814  AYWKGNAWVSNNRGDLMKCNKSDKNDWKARLYQVNWVDESKKGFNRTNLADQCTHRYKIY 635
            AYWKGN  +   R +L KCNK+ + DW AR+Y ++W  E   GFN ++LA QCTHRYKIY
Sbjct: 361  AYWKGNINLGLARHELSKCNKTSEQDWNARIYDIDWRQEIHSGFNSSDLASQCTHRYKIY 420

Query: 634  IEGRAWSVSEKYILACDSMTLLVKPQYYDFFTRSLQPLVHYWPVNKNNKCRSVKFAVDWG 455
             EG  WSVSEKYILACDS+TLL  P YYDFFTRSLQP+VHYWP+   + C+S+KFA +W 
Sbjct: 421  TEGVTWSVSEKYILACDSVTLLANPHYYDFFTRSLQPMVHYWPLKLKDMCKSIKFATEWC 480

Query: 454  NSHPKKAQRIGRAGSKFIQEGLNMKSVYDYMYHTLNEYAKLMKYKPTVPPQATQVCSETM 275
            N+H +KAQ I +AGS F+QE L MK VYDYM+H L+ YAKL+K+KP+VPP A + C ETM
Sbjct: 481  NNHTEKAQEIAKAGSSFVQEKLKMKFVYDYMFHLLSMYAKLLKFKPSVPPGAVEFCPETM 540

Query: 274  ACSEHQELNKRFKIESMVMNPSDTNPCILAPSYSNNEVHAIRDKQESTRKQVELWENNG 98
             C   + L K +KI+SMV +PSDT PC + P Y   E+  + +K++   KQVE WE +G
Sbjct: 541  VCPV-KGLEKDYKIQSMVRSPSDTGPCTMPPPYDPAELKDVLEKKDHVMKQVETWEESG 598



 Score = 80.5 bits (197), Expect(2) = 2e-15
 Identities = 32/53 (60%), Positives = 46/53 (86%)
 Frame = -2

Query: 1192 ANLRIIIVDGKLYTQKYSGVFQTRDVFTIWGIVQLLKLYPGKVPDVDFMFECG 1034
            A++R+++VDGK++ +KY  VFQTR V TIW I+QLL++YPGK+PD+D +FECG
Sbjct: 17   AHIRVVVVDGKVFMEKYEWVFQTRHVLTIWCILQLLRMYPGKLPDLDLIFECG 69



 Score = 30.0 bits (66), Expect(2) = 2e-15
 Identities = 16/34 (47%), Positives = 20/34 (58%)
 Frame = -3

Query: 1005 TWERMHLFHRHCFIIVAMIQPWI*FSPIGLSGVG 904
            T + M L H+HCFI V M++  I  S  G  GVG
Sbjct: 80   TKDLMPLLHQHCFITVEMMKHLILSSGTGHFGVG 113


>ref|XP_002273350.2| PREDICTED: O-glucosyltransferase rumi homolog [Vitis vinifera]
          Length = 509

 Score =  558 bits (1437), Expect = e-156
 Identities = 257/467 (55%), Positives = 330/467 (70%), Gaps = 1/467 (0%)
 Frame = -2

Query: 1504 SVTLFCICLFIGALVATQMLDVPIFTGAPLQKSSPTTKIHKFTYQLQCS-GNTTQTCPXX 1328
            +V++F I L IG  ++T+      F  A   +SS      +F Y + CS GN T+TCP  
Sbjct: 36   NVSVFFIILLIGTFLSTRWSG-DSFRTAWNYRSSTKPHYKRFEYSMNCSEGNMTKTCPVT 94

Query: 1327 XXXXXXXXXTSASQCPEYFRWIYEDLKPWEKTGLTKEIVESGMHKANLRIIIVDGKLYTQ 1148
                      S   CPEYFRWIYEDLKPW +TG+T+++VE     A++R+++VDGK+YT+
Sbjct: 95   LLTTFEPSNLSTGTCPEYFRWIYEDLKPWTETGITRDMVERAKTPAHIRVVVVDGKVYTE 154

Query: 1147 KYSGVFQTRDVFTIWGIVQLLKLYPGKVPDVDFMFECGDMPMIHKRDYMGANASIPPPLF 968
            KY  VFQTRDVFTIWGI+Q+L++YPGK+PD D MFECGD P+I K DY G NA+ PP LF
Sbjct: 155  KYKWVFQTRDVFTIWGILQVLRMYPGKLPDFDLMFECGDKPVIKKHDYQGLNATAPP-LF 213

Query: 967  HYCGDDSTLDLVFPDWSFWGWPELNIRPWVSLXXXXXXXXXXXXXXKREPYAYWKGNAWV 788
            HYCGDD TLD+VFPDWSFWGWPE+ I+PW +L               REPYAYWKGN  +
Sbjct: 214  HYCGDDETLDIVFPDWSFWGWPEIRIKPWSTLRKDLREGNNKTKWVDREPYAYWKGNFKM 273

Query: 787  SNNRGDLMKCNKSDKNDWKARLYQVNWVDESKKGFNRTNLADQCTHRYKIYIEGRAWSVS 608
               R +L KC+K+++ DW AR+Y ++W+ E + GF   +L+ QCTH+YKIY EG AWSVS
Sbjct: 274  GVTRHELSKCSKTNEQDWNARIYNMDWLQEMQNGFKSADLSTQCTHKYKIYAEGAAWSVS 333

Query: 607  EKYILACDSMTLLVKPQYYDFFTRSLQPLVHYWPVNKNNKCRSVKFAVDWGNSHPKKAQR 428
            EKYILACDS+TLLVKPQYYDFFTRSLQPLVHYWP+   + C+S+KFA +W N+H +KA  
Sbjct: 334  EKYILACDSVTLLVKPQYYDFFTRSLQPLVHYWPIKLKDMCKSIKFATEWCNNHTQKAHE 393

Query: 427  IGRAGSKFIQEGLNMKSVYDYMYHTLNEYAKLMKYKPTVPPQATQVCSETMACSEHQELN 248
            I  AGS F+QE L MK VYDYM+H L+ YAKL KYKPTVPP A +VC ETM C   + L 
Sbjct: 394  IRNAGSSFVQEELRMKFVYDYMFHLLSAYAKLFKYKPTVPPGAVEVCPETMVCPV-KGLQ 452

Query: 247  KRFKIESMVMNPSDTNPCILAPSYSNNEVHAIRDKQESTRKQVELWE 107
            K++KI+SMV +PSDT PC++ P Y   E+  + ++++   KQVE+ E
Sbjct: 453  KKYKIQSMVKSPSDTGPCVMPPPYDPAELRDMLERKDHVMKQVEMLE 499