BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00013819
         (1472 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ACF17660.1| putative isopropylmalate synthase [Capsicum annuum]    733   0.0  
sp|O04973.1|LEU1A_SOLPN RecName: Full=2-isopropylmalate synthase...   732   0.0  
sp|O04974.1|LEU1B_SOLPN RecName: Full=2-isopropylmalate synthase...   711   0.0  
ref|XP_002268942.2| PREDICTED: 2-isopropylmalate synthase 2, chl...   706   0.0  
ref|XP_002307122.1| predicted protein [Populus trichocarpa] gi|2...   687   0.0  

>gb|ACF17660.1| putative isopropylmalate synthase [Capsicum annuum]
          Length = 602

 Score =  733 bits (1893), Expect = 0.0
 Identities = 367/446 (82%), Positives = 411/446 (92%), Gaps = 6/446 (1%)
 Frame = +1

Query: 151  TLLHLKPNKP------PILRCSSSLTRPEYVPNHIPDPHYIRIFDTTLRDGEQSPGATMT 312
            TLL+ KP+ P      PI+RCS    RPEY P+HI DP+YIRIFDTTLRDGEQSPGATMT
Sbjct: 22   TLLNFKPSIPTHSFSIPIVRCSIRC-RPEYTPSHISDPNYIRIFDTTLRDGEQSPGATMT 80

Query: 313  TKEKLDVARQLAKLGVDIIEAGFPAASDADLEAVKMIAMEIGNGVSGGDYVPVICGLARC 492
            TKEKLDVARQLAKLGVDIIEAGFPA+S+ADLEAVK+IA E+GNGV    +VPVICGLARC
Sbjct: 81   TKEKLDVARQLAKLGVDIIEAGFPASSEADLEAVKLIAKEVGNGVYEEGHVPVICGLARC 140

Query: 493  NKKDIDSAWEAVRFAKKPRIHTFIATSEIHMKYKLKKTKEEVVERARSMVAYARSLGCDD 672
            NK+DID AWEAV++AKKPRIHTFIATSEIHMK+KLK +++EV+E+ARSMVAYARS+GC D
Sbjct: 141  NKRDIDKAWEAVKYAKKPRIHTFIATSEIHMKFKLKMSRDEVIEKARSMVAYARSIGCQD 200

Query: 673  VEFSPEDAGRSEREFLYHILEEVIKAGATTLNIPDTVGYTVPSEFGQLIADIKANTPGIE 852
            VEFSPEDAGRS+ EFLYHIL EVIKAGATTLNIPDTVGYTVP EFGQLIA+IKANTPG+E
Sbjct: 201  VEFSPEDAGRSDPEFLYHILGEVIKAGATTLNIPDTVGYTVPEEFGQLIANIKANTPGVE 260

Query: 853  NVIISTHCQNDLGLSTANTLAGACAGARQLEVTVNGIGERAGNASLEEVVMALKCRGEQV 1032
            +VIISTHCQNDLGLSTANTLAGACAGARQLEVT+NGIGERAGNASLEEVVMALKCRGEQV
Sbjct: 261  DVIISTHCQNDLGLSTANTLAGACAGARQLEVTINGIGERAGNASLEEVVMALKCRGEQV 320

Query: 1033 MGGLYTAIDTKHIVMASKMVEEYSGLLVQPHKAIVGANAFAHESGIHQDGMLKHKNTYEI 1212
            +GGLYT I+T+HI+M+SKMVEEY+GL VQPHKAIVGANAFAHESGIHQDGMLKHK+TYEI
Sbjct: 321  LGGLYTGINTQHILMSSKMVEEYTGLHVQPHKAIVGANAFAHESGIHQDGMLKHKDTYEI 380

Query: 1213 ISPEDIGLYRSDESGIVLGKLSGRHAVRSKLLELGYVIDGKEFEDLFRNFKSVAEKKKII 1392
            +SPEDIGL R++ESGIVLGKLSGRHA+++K+LELGY I+GKE +DLF  FKSVAEKKK I
Sbjct: 381  MSPEDIGLNRANESGIVLGKLSGRHALQAKMLELGYEIEGKELDDLFWRFKSVAEKKKKI 440

Query: 1393 TDDDLIALVSDEVFQPQVVWKLGDVQ 1470
            TDDDLIAL+SDEVFQPQ VW+L +VQ
Sbjct: 441  TDDDLIALMSDEVFQPQFVWQLQNVQ 466


>sp|O04973.1|LEU1A_SOLPN RecName: Full=2-isopropylmalate synthase A; AltName: Full=Alpha-IPM
            synthase A; AltName: Full=Alpha-isopropylmalate synthase
            A gi|2213882|gb|AAB61598.1| 2-isopropylmalate synthase
            [Solanum pennellii]
          Length = 589

 Score =  732 bits (1890), Expect = 0.0
 Identities = 364/428 (85%), Positives = 396/428 (92%)
 Frame = +1

Query: 187  LRCSSSLTRPEYVPNHIPDPHYIRIFDTTLRDGEQSPGATMTTKEKLDVARQLAKLGVDI 366
            +RCS S  RPEYVP+ I DP Y+RIFDTTLRDGEQSPGATMTTKEKLDVARQLAKLGVDI
Sbjct: 26   IRCSISNRRPEYVPSKISDPKYVRIFDTTLRDGEQSPGATMTTKEKLDVARQLAKLGVDI 85

Query: 367  IEAGFPAASDADLEAVKMIAMEIGNGVSGGDYVPVICGLARCNKKDIDSAWEAVRFAKKP 546
            IEAGFPA+S+AD E+VK+IA EIGN      +VPVICGL+RCNK DID AWEAV++AKKP
Sbjct: 86   IEAGFPASSEADFESVKLIAEEIGNNTDENGFVPVICGLSRCNKSDIDKAWEAVKYAKKP 145

Query: 547  RIHTFIATSEIHMKYKLKKTKEEVVERARSMVAYARSLGCDDVEFSPEDAGRSEREFLYH 726
            R+HTFIATSEIHMKYKLK ++E+VVE+ARSMVAYARSLGC+DVEFSPEDAGRS+REFLY 
Sbjct: 146  RVHTFIATSEIHMKYKLKMSREQVVEKARSMVAYARSLGCEDVEFSPEDAGRSDREFLYD 205

Query: 727  ILEEVIKAGATTLNIPDTVGYTVPSEFGQLIADIKANTPGIENVIISTHCQNDLGLSTAN 906
            IL EVIKAGATTLNIPDTVGYTVPSEFGQLI DIKANTPGIENVIISTHCQNDLGLSTAN
Sbjct: 206  ILGEVIKAGATTLNIPDTVGYTVPSEFGQLITDIKANTPGIENVIISTHCQNDLGLSTAN 265

Query: 907  TLAGACAGARQLEVTVNGIGERAGNASLEEVVMALKCRGEQVMGGLYTAIDTKHIVMASK 1086
            TLAGACAGARQLEVT+NGIGERAGNASLEEVVMALKCRGEQV+GGLYT I+T+HIV +SK
Sbjct: 266  TLAGACAGARQLEVTINGIGERAGNASLEEVVMALKCRGEQVLGGLYTGINTQHIVPSSK 325

Query: 1087 MVEEYSGLLVQPHKAIVGANAFAHESGIHQDGMLKHKNTYEIISPEDIGLYRSDESGIVL 1266
            MVEEYSGL VQPHKAIVGANAFAHESGIHQDGMLKHK+TYEIISP+D+GL RS+E+GIVL
Sbjct: 326  MVEEYSGLQVQPHKAIVGANAFAHESGIHQDGMLKHKDTYEIISPDDVGLSRSNEAGIVL 385

Query: 1267 GKLSGRHAVRSKLLELGYVIDGKEFEDLFRNFKSVAEKKKIITDDDLIALVSDEVFQPQV 1446
            GKLSGRHA++SK+LELGY IDGKE EDLF  FKSVAEKKK ITDDDLIAL+SDEV QP V
Sbjct: 386  GKLSGRHALKSKMLELGYDIDGKELEDLFWRFKSVAEKKKKITDDDLIALMSDEVLQPNV 445

Query: 1447 VWKLGDVQ 1470
             WKLGDVQ
Sbjct: 446  YWKLGDVQ 453


>sp|O04974.1|LEU1B_SOLPN RecName: Full=2-isopropylmalate synthase B; AltName: Full=Alpha-IPM
            synthase B; AltName: Full=Alpha-isopropylmalate synthase
            B gi|2213884|gb|AAB61599.1| 2-isopropylmalate synthase
            [Solanum pennellii]
          Length = 612

 Score =  711 bits (1835), Expect = 0.0
 Identities = 353/430 (82%), Positives = 393/430 (91%)
 Frame = +1

Query: 181  PILRCSSSLTRPEYVPNHIPDPHYIRIFDTTLRDGEQSPGATMTTKEKLDVARQLAKLGV 360
            P++RCS     PEY P+HIPDP+Y+RIFDTTLRDGEQSPGATMTTKEKLDVARQ AKLGV
Sbjct: 48   PVVRCSIRRI-PEYTPSHIPDPNYVRIFDTTLRDGEQSPGATMTTKEKLDVARQSAKLGV 106

Query: 361  DIIEAGFPAASDADLEAVKMIAMEIGNGVSGGDYVPVICGLARCNKKDIDSAWEAVRFAK 540
            DIIEAGFPA+S+ADLEAVK+IA E+GNGV   +YVPVICGLARCNKKDID AWEAV++AK
Sbjct: 107  DIIEAGFPASSEADLEAVKLIAKEVGNGVYEEEYVPVICGLARCNKKDIDKAWEAVKYAK 166

Query: 541  KPRIHTFIATSEIHMKYKLKKTKEEVVERARSMVAYARSLGCDDVEFSPEDAGRSEREFL 720
            KPRIHTFIATSE+HM YKLK ++++VVE+ARSMVAYARS+GC+DVEFSPEDAGRS+ EFL
Sbjct: 167  KPRIHTFIATSEVHMNYKLKMSRDQVVEKARSMVAYARSIGCEDVEFSPEDAGRSDPEFL 226

Query: 721  YHILEEVIKAGATTLNIPDTVGYTVPSEFGQLIADIKANTPGIENVIISTHCQNDLGLST 900
            YHIL EVIKAGATTLNIPDTVGYTVP EFGQLIA IKANTPG+E+VIISTHCQNDLGLST
Sbjct: 227  YHILGEVIKAGATTLNIPDTVGYTVPEEFGQLIAKIKANTPGVEDVIISTHCQNDLGLST 286

Query: 901  ANTLAGACAGARQLEVTVNGIGERAGNASLEEVVMALKCRGEQVMGGLYTAIDTKHIVMA 1080
            ANTLAGACAGARQLEVT+NGIGERAGNASLEEVVMALKCRGEQV+GGLYT I+T+HI+M+
Sbjct: 287  ANTLAGACAGARQLEVTINGIGERAGNASLEEVVMALKCRGEQVLGGLYTGINTQHILMS 346

Query: 1081 SKMVEEYSGLLVQPHKAIVGANAFAHESGIHQDGMLKHKNTYEIISPEDIGLYRSDESGI 1260
            SKMVE  SGL VQPHKAIVGANAF HESGIHQDGMLKHK+TYEIISPEDIGL R++ESGI
Sbjct: 347  SKMVEGISGLHVQPHKAIVGANAFVHESGIHQDGMLKHKDTYEIISPEDIGLNRANESGI 406

Query: 1261 VLGKLSGRHAVRSKLLELGYVIDGKEFEDLFRNFKSVAEKKKIITDDDLIALVSDEVFQP 1440
            V GKLSG    + K+LELGY I+GKE +DLF  FKSVAEKKK ITDDDL+AL+SDEVFQP
Sbjct: 407  VFGKLSGVMLCKPKMLELGYEIEGKELDDLFWRFKSVAEKKKKITDDDLVALMSDEVFQP 466

Query: 1441 QVVWKLGDVQ 1470
            Q VW+L +VQ
Sbjct: 467  QFVWQLQNVQ 476


>ref|XP_002268942.2| PREDICTED: 2-isopropylmalate synthase 2, chloroplastic-like [Vitis
            vinifera]
          Length = 628

 Score =  706 bits (1823), Expect = 0.0
 Identities = 347/423 (82%), Positives = 387/423 (91%)
 Frame = +1

Query: 202  SLTRPEYVPNHIPDPHYIRIFDTTLRDGEQSPGATMTTKEKLDVARQLAKLGVDIIEAGF 381
            S  RPEY+PN I DP+Y+RIFDTTLRDGEQSPGATMT+KEKLD+ARQLAKLGVDIIEAGF
Sbjct: 69   SALRPEYIPNRIDDPNYVRIFDTTLRDGEQSPGATMTSKEKLDIARQLAKLGVDIIEAGF 128

Query: 382  PAASDADLEAVKMIAMEIGNGVSGGDYVPVICGLARCNKKDIDSAWEAVRFAKKPRIHTF 561
            PAAS  DL+AVK+IA E+GN V    +VPVICGLARCN+ DI +AWEAV++AK+PRIHTF
Sbjct: 129  PAASKDDLDAVKIIAKEVGNAVDENGHVPVICGLARCNRNDISTAWEAVKYAKRPRIHTF 188

Query: 562  IATSEIHMKYKLKKTKEEVVERARSMVAYARSLGCDDVEFSPEDAGRSEREFLYHILEEV 741
            IATSEIHMKYKLKK+++EV+E AR MV YARSLGCDDVEFSPEDAGRSEREFLY IL EV
Sbjct: 189  IATSEIHMKYKLKKSEKEVIEIAREMVKYARSLGCDDVEFSPEDAGRSEREFLYKILGEV 248

Query: 742  IKAGATTLNIPDTVGYTVPSEFGQLIADIKANTPGIENVIISTHCQNDLGLSTANTLAGA 921
            IKAGATTLNIPDTVGYTVP+EFGQLIADIK+NTPGIENVIISTHCQNDLGLSTANT+AGA
Sbjct: 249  IKAGATTLNIPDTVGYTVPNEFGQLIADIKSNTPGIENVIISTHCQNDLGLSTANTIAGA 308

Query: 922  CAGARQLEVTVNGIGERAGNASLEEVVMALKCRGEQVMGGLYTAIDTKHIVMASKMVEEY 1101
            CAGARQ+EVT+NGIGERAGNASLEEVVMALKCRGE+ +GGLYT I+ +HIV  SKMV EY
Sbjct: 309  CAGARQVEVTINGIGERAGNASLEEVVMALKCRGEKALGGLYTGINIRHIVKTSKMVAEY 368

Query: 1102 SGLLVQPHKAIVGANAFAHESGIHQDGMLKHKNTYEIISPEDIGLYRSDESGIVLGKLSG 1281
            SGL VQPHKAIVGANAFAHESGIHQDGMLKHK TYEIISPEDIGL+RS+ESGIVLGKLSG
Sbjct: 369  SGLQVQPHKAIVGANAFAHESGIHQDGMLKHKETYEIISPEDIGLFRSNESGIVLGKLSG 428

Query: 1282 RHAVRSKLLELGYVIDGKEFEDLFRNFKSVAEKKKIITDDDLIALVSDEVFQPQVVWKLG 1461
            RHA++S+LLELGY I+G E +D+F  FK VAEKKK +TD D+IALVSDE+FQP+V WKLG
Sbjct: 429  RHALKSRLLELGYEIEGMELDDIFWRFKEVAEKKKNLTDVDVIALVSDEIFQPKVFWKLG 488

Query: 1462 DVQ 1470
            D+Q
Sbjct: 489  DLQ 491


>ref|XP_002307122.1| predicted protein [Populus trichocarpa] gi|222856571|gb|EEE94118.1|
            predicted protein [Populus trichocarpa]
          Length = 558

 Score =  687 bits (1772), Expect = 0.0
 Identities = 342/420 (81%), Positives = 379/420 (90%)
 Frame = +1

Query: 211  RPEYVPNHIPDPHYIRIFDTTLRDGEQSPGATMTTKEKLDVARQLAKLGVDIIEAGFPAA 390
            RP YVPN IPDP+Y+RIFDTTLRDGEQSPGAT+T+KEKLD+ARQLAKLGVDIIEAGFPAA
Sbjct: 1    RPVYVPNKIPDPNYVRIFDTTLRDGEQSPGATLTSKEKLDIARQLAKLGVDIIEAGFPAA 60

Query: 391  SDADLEAVKMIAMEIGNGVSGGDYVPVICGLARCNKKDIDSAWEAVRFAKKPRIHTFIAT 570
            S  D EAVKMIA E+GN V    YVPVICGL+RCN+KDI +AWEAV+ AK+PRIHTFIAT
Sbjct: 61   SKDDFEAVKMIANEVGNQVDSEGYVPVICGLSRCNEKDIRAAWEAVKNAKRPRIHTFIAT 120

Query: 571  SEIHMKYKLKKTKEEVVERARSMVAYARSLGCDDVEFSPEDAGRSEREFLYHILEEVIKA 750
            S IHM+YKL+K+KEEVVE A SMV +ARSLGCDDVEFSPEDAGRSEREFLYHIL EVIKA
Sbjct: 121  SGIHMEYKLRKSKEEVVEIASSMVRFARSLGCDDVEFSPEDAGRSEREFLYHILGEVIKA 180

Query: 751  GATTLNIPDTVGYTVPSEFGQLIADIKANTPGIENVIISTHCQNDLGLSTANTLAGACAG 930
            GATTLNIPDTVG T PSEFGQLIADIKANTPGIENVIISTHCQNDLGLSTANTL+GACAG
Sbjct: 181  GATTLNIPDTVGITTPSEFGQLIADIKANTPGIENVIISTHCQNDLGLSTANTLSGACAG 240

Query: 931  ARQLEVTVNGIGERAGNASLEEVVMALKCRGEQVMGGLYTAIDTKHIVMASKMVEEYSGL 1110
            ARQ+EVT+NGIGERAGNASLEEVVM +KCRGE V+GGLYT I+T+HI MASKMVEEY+GL
Sbjct: 241  ARQVEVTINGIGERAGNASLEEVVMVIKCRGEHVLGGLYTGINTRHITMASKMVEEYTGL 300

Query: 1111 LVQPHKAIVGANAFAHESGIHQDGMLKHKNTYEIISPEDIGLYRSDESGIVLGKLSGRHA 1290
             VQPHKAIVGANAFAHESGIHQDGMLKHK TYEIISPEDIGL RS+++GIVLGKLSGRHA
Sbjct: 301  HVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGLERSNDAGIVLGKLSGRHA 360

Query: 1291 VRSKLLELGYVIDGKEFEDLFRNFKSVAEKKKIITDDDLIALVSDEVFQPQVVWKLGDVQ 1470
            ++ +L ELGY +D  +  ++F  FK+VAE KK +TD DLIALVSDEVFQP+ VWKL D+Q
Sbjct: 361  LKDRLNELGYELDDAQLGNIFWRFKAVAETKKRVTDADLIALVSDEVFQPENVWKLHDLQ 420