BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00013798
         (1834 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAF46296.1| cytochrome P450 [Ipomoea nil]                         536   e-150
ref|XP_002279670.1| PREDICTED: cytochrome P450 86B1 [Vitis vinif...   491   e-136
gb|AEI59774.1| cytochrome P450 [Helianthus annuus]                    488   e-135
ref|XP_002334340.1| cytochrome P450 [Populus trichocarpa] gi|222...   482   e-133
ref|XP_002321857.1| cytochrome P450 [Populus trichocarpa] gi|222...   482   e-133

>dbj|BAF46296.1| cytochrome P450 [Ipomoea nil]
          Length = 525

 Score =  536 bits (1381), Expect = e-150
 Identities = 265/486 (54%), Positives = 340/486 (69%), Gaps = 15/486 (3%)
 Frame = +2

Query: 149  DWPIFRMTPDLIKNGHRLHEFMTEVLKQNHNTFQFKGPA-LSNLDMLCTCEPADIHYILS 325
            +WP+  M P L+KN HR+HEF T+VLK +  TF+FKGPA  +NL ML T +PA+IH++LS
Sbjct: 41   NWPLVGMMPALLKNAHRVHEFATDVLKHSGGTFEFKGPAAFANLSMLATSDPANIHHVLS 100

Query: 326  KNFANYPKGPEFRKIFDILGDGIFNADAYLWELHRKNTTMLINQGEFYKMVIDTTTRKVK 505
            KNF+NYPKGPEFRKIFDILGDGIFN D+ LWELHRK T   +N  +FY ++      KV+
Sbjct: 101  KNFSNYPKGPEFRKIFDILGDGIFNVDSQLWELHRKTTLSFLNHAQFYTLLQTNVWHKVE 160

Query: 506  NGLIPVLAYVSKHGIEVDLQELFQRLAFDNICSILLDHDPQSLSISLPYLPCEKAFTATE 685
             GL+PVL + ++ G E DLQ++FQR  FD I  ++L HDP+SL + LPY+PCEKAF    
Sbjct: 161  KGLLPVLDWFAEEGAEFDLQDVFQRFTFDAISKLVLHHDPESLCLGLPYVPCEKAFNHAV 220

Query: 686  EAILYRHILPESIWKLQSLIGFGKEKGLNKARKAFDEFIYRCISM--RRQQAVSGQFSTS 859
            +A+LYRHI PES WKLQ+ +G GKEK L +A +AFDEFIY  +     R   ++     +
Sbjct: 221  DALLYRHIFPESFWKLQNWLGIGKEKKLIQAYRAFDEFIYSALKRLEERMMMITPNIDNN 280

Query: 860  GED-KLDSLTAHMEAYKERNGNQEKLL---------RDTLLNLMIAGRDTTSTALSWFFW 1009
             +D  L   TA+++AYK++   Q  LL         RDT L+LM AGRDTTS+ L+WFF+
Sbjct: 281  SDDHHLSLFTAYVQAYKQQEQPQAALLTTTTTTKFPRDTFLSLMFAGRDTTSSTLTWFFY 340

Query: 1010 LLSKNPSVEFKIQEEI--KLNLQSNGKESFHKREELSKLVYLHGALCETLRLFPPVALEH 1183
            LL++NP V+   ++EI  KLNL+   K  F K EE  KLVYLH ALCETLRLFPP+A++H
Sbjct: 341  LLAQNPLVQANARDEIENKLNLKQEIKR-FFKLEECHKLVYLHAALCETLRLFPPLAIQH 399

Query: 1184 KAPIQADVLPSGHHVNKDSKIVLSFYSMGRMESIWGKDCLDFKPERWITNNGGIKHEPSY 1363
            K     DVLPSGH V  +++I+L FYS GRM+SIWG+DC++FKPERWI+ NG IKHEPSY
Sbjct: 400  KVSTAMDVLPSGHLVKPNTRIILLFYSSGRMDSIWGEDCMEFKPERWISGNGRIKHEPSY 459

Query: 1364 KFPAFNAGPRTCLGKEMAFXXXXXXXXXXXXXXXXKVVDGHPIVPGDSIIMHIKHGLKTT 1543
            KFPAFNAGPRTCLGKEM+F                +VVD H + P DSII+  KHGLK  
Sbjct: 460  KFPAFNAGPRTCLGKEMSFVQMKMIAAIILHHYEFQVVDSHSVSPSDSIIIQAKHGLKVK 519

Query: 1544 ISNKSV 1561
             + +S+
Sbjct: 520  FTKRSM 525


>ref|XP_002279670.1| PREDICTED: cytochrome P450 86B1 [Vitis vinifera]
          Length = 502

 Score =  491 bits (1265), Expect = e-136
 Identities = 241/471 (51%), Positives = 318/471 (67%), Gaps = 2/471 (0%)
 Frame = +2

Query: 149  DWPIFRMTPDLIKNGHRLHEFMTEVLKQNHNTFQFKGPALSNLDMLCTCEPADIHYILSK 328
            +WP+  M P + +N  ++ +  TEVL+Q+  TF  KGP  +N+DML T +PA++HYI+S 
Sbjct: 31   NWPLVGMLPWIFRNIQQIQDRCTEVLEQSSGTFLLKGPWFANMDMLITSDPANVHYIMST 90

Query: 329  NFANYPKGPEFRKIFDILGDGIFNADAYLWELHRKNTTMLINQGEFYKMVIDTTTRKVKN 508
            NF+N+PKGPEF KIFD+LG+GIFN+D+ LW   RK   +LIN  +F + ++ TT  KV  
Sbjct: 91   NFSNFPKGPEFLKIFDVLGEGIFNSDSDLWRKQRKLAQVLINDRQFQQFLVKTTENKVDE 150

Query: 509  GLIPVLAYVSKHGIEVDLQELFQRLAFDNICSILLDHDPQSLSISLPYLPCEKAFTATEE 688
            GLIPVL +VSK G+ VDLQ+LFQR  FD  C ++   D + LSI  P +   KA    EE
Sbjct: 151  GLIPVLEHVSKQGLLVDLQDLFQRFTFDTTCKLITGFDTKCLSIEFPRVLFAKAMDDVEE 210

Query: 689  AILYRHILPESIWKLQSLIGFGKEKGLNKARKAFDEFIYRCISMRRQQAVSGQFS-TSGE 865
            AI +RH++PES+WKLQ  +G G+EK L+K  +  D  I   ISM+ ++   G       E
Sbjct: 211  AIFFRHVVPESLWKLQRWLGIGEEKKLSKGWETIDNVIAEYISMKHEELSKGIAKLQEDE 270

Query: 866  DKLDSLTAHMEAYKERNGNQEKLLRDTLLNLMIAGRDTTSTALSWFFWLLSKNPSVEFKI 1045
            +  D LT++M           K LRDT++N ++AGRDTTS AL+WFFWL+SKNP VE KI
Sbjct: 271  EGTDLLTSYMNEDSTMELKSNKFLRDTIVNFLLAGRDTTSAALTWFFWLVSKNPLVEAKI 330

Query: 1046 QEEIKLNL-QSNGKESFHKREELSKLVYLHGALCETLRLFPPVALEHKAPIQADVLPSGH 1222
            +EE+K  + + + K    + EEL+KLVYLHGALCETLRL+PPV  +HKAP QADVLPSGH
Sbjct: 331  REELKETIPEKDQKWHIFRTEELNKLVYLHGALCETLRLYPPVPFQHKAPHQADVLPSGH 390

Query: 1223 HVNKDSKIVLSFYSMGRMESIWGKDCLDFKPERWITNNGGIKHEPSYKFPAFNAGPRTCL 1402
             V+   KI+ S Y+MGRM +IWGKDC +FKPERWIT  GGIK+EPSYKF AFNAGPRTCL
Sbjct: 391  RVHPKMKILFSLYAMGRMTTIWGKDCFEFKPERWITEQGGIKYEPSYKFFAFNAGPRTCL 450

Query: 1403 GKEMAFXXXXXXXXXXXXXXXXKVVDGHPIVPGDSIIMHIKHGLKTTISNK 1555
            GK +AF                ++V GHP+ P  SII+H+KH L   ++ +
Sbjct: 451  GKGVAFTQMKAVAAAIIHNYNVQLVQGHPVAPNVSIILHMKHELMVRVTKR 501


>gb|AEI59774.1| cytochrome P450 [Helianthus annuus]
          Length = 505

 Score =  488 bits (1256), Expect = e-135
 Identities = 228/470 (48%), Positives = 328/470 (69%)
 Frame = +2

Query: 149  DWPIFRMTPDLIKNGHRLHEFMTEVLKQNHNTFQFKGPALSNLDMLCTCEPADIHYILSK 328
            +WPI  M P ++ N H+LH++ TE+L Q+  TF  KGP L+N+DML T +P DIH+ILSK
Sbjct: 36   NWPILGMIPGVLVNAHQLHDYTTELLIQSRGTFMLKGPWLANMDMLLTTDPLDIHHILSK 95

Query: 329  NFANYPKGPEFRKIFDILGDGIFNADAYLWELHRKNTTMLINQGEFYKMVIDTTTRKVKN 508
            NF+NYPKG +F+KIFDILGDGI NAD  +WE+  K    ++N   F   +      KV+N
Sbjct: 96   NFSNYPKGDKFQKIFDILGDGILNADGKIWEISHKVIFSVLNHVGFQSKMETIIWDKVEN 155

Query: 509  GLIPVLAYVSKHGIEVDLQELFQRLAFDNICSILLDHDPQSLSISLPYLPCEKAFTATEE 688
            GL+P+L  + + G E+DLQ++FQR AFD +  +LLD+D +SLS++ PY P  +AFT +EE
Sbjct: 156  GLLPILESICERGTEMDLQDIFQRFAFDIVFKLLLDNDQESLSLNFPYNPYSEAFTDSEE 215

Query: 689  AILYRHILPESIWKLQSLIGFGKEKGLNKARKAFDEFIYRCISMRRQQAVSGQFSTSGED 868
            A+L RH+ P  +WKLQ ++  GKEK L+ A K+ D+FIY+C++ +     +         
Sbjct: 216  AVLLRHVTPPFLWKLQQILRVGKEKKLSDAWKSLDQFIYKCLAQKEMDYNNMNHEAEKFT 275

Query: 869  KLDSLTAHMEAYKERNGNQEKLLRDTLLNLMIAGRDTTSTALSWFFWLLSKNPSVEFKIQ 1048
              D++   ++ + + + +  K LRDT  NL  AG+DT S+AL WFF+LL+ NP+VE KI 
Sbjct: 276  FFDAIMRELKDHIDTSMDCTKFLRDTFFNLTAAGKDTISSALCWFFYLLATNPTVEDKIL 335

Query: 1049 EEIKLNLQSNGKESFHKREELSKLVYLHGALCETLRLFPPVALEHKAPIQADVLPSGHHV 1228
            EE++ +L+ +  + ++   EL K+VYLHGALCETLRL+PPV   +K+P+Q D+LPSGH V
Sbjct: 336  EEMQTHLEVSALKRWN-ATELDKMVYLHGALCETLRLYPPVPFNNKSPLQPDILPSGHQV 394

Query: 1229 NKDSKIVLSFYSMGRMESIWGKDCLDFKPERWITNNGGIKHEPSYKFPAFNAGPRTCLGK 1408
            ++++ I+L FYSMGRM SIWG+DC++FKPERWI+++GGIKH PSYKFP FNAGPR CLGK
Sbjct: 395  DQNTNIILCFYSMGRMRSIWGEDCMEFKPERWISSSGGIKHVPSYKFPTFNAGPRACLGK 454

Query: 1409 EMAFXXXXXXXXXXXXXXXXKVVDGHPIVPGDSIIMHIKHGLKTTISNKS 1558
             MAF                ++V+GH ++P DS+++H+K+GLK  ++ +S
Sbjct: 455  NMAFSELKIVATTIIYHYHIQLVEGHLVLPADSMVLHMKYGLKVKLNRRS 504


>ref|XP_002334340.1| cytochrome P450 [Populus trichocarpa] gi|222871210|gb|EEF08341.1|
            cytochrome P450 [Populus trichocarpa]
          Length = 506

 Score =  482 bits (1241), Expect = e-133
 Identities = 238/475 (50%), Positives = 318/475 (66%), Gaps = 6/475 (1%)
 Frame = +2

Query: 149  DWPIFRMTPDLIKNGHRLHEFMTEVLKQNHNTFQFKGPALSNLDMLCTCEPADIHYILSK 328
            +WP+  M P L+   HR H+++T+VL+Q+  TFQFKGP   ++D+L T +PA+IHYI S 
Sbjct: 32   NWPLIGMLPGLLFQVHRFHDWVTDVLEQSKCTFQFKGPWFGDMDILVTADPANIHYITSS 91

Query: 329  NFANYPKGPEFRKIFDILGDGIFNADAYLWELHRKNTTMLINQGEFYKMVIDTTTRKVKN 508
            NF+N+ KG +F K FDILGDGI NAD  LW+  RK    L+N   FY+ ++ T   KV+ 
Sbjct: 92   NFSNFEKGSDFNKRFDILGDGIINADRDLWKSQRKVAQALVNHRLFYQRLVKTIQDKVER 151

Query: 509  GLIPVLAYVSKHGIEVDLQELFQRLAFDNICSILLDHDPQSLSISLPYLPCEKAFTATEE 688
            GLIPVL +V K  + +DLQ+LFQR  FD  C I+   DP  LSI LP +   KA     E
Sbjct: 152  GLIPVLEHVYKQSLVLDLQDLFQRFTFDATCVIVTGFDPGCLSIDLPEVAFSKAMDNAME 211

Query: 689  AILYRHILPESIWKLQSLIGFGKEKGLNKARKAFDEFIYRCISMRRQQAVSG-QFSTSGE 865
            AI YRH+LPES W+LQ  +G GKEK L KAR+  D  I   ISM+R++   G +      
Sbjct: 212  AIFYRHVLPESTWRLQRWLGIGKEKKLKKARETLDRIIAEIISMKREELSKGNRLMEEDG 271

Query: 866  DKLDSLTAHM-EAYKERNGNQEKLLRDTLLNLMIAGRDTTSTALSWFFWLLSKNPSVEFK 1042
            + +D LT++M E Y     + ++ LRDT++  M+AGRDT S+ LSWFFWL+SKNP+   K
Sbjct: 272  EGIDLLTSYMSEDYNMGFKSDDEFLRDTIVTFMLAGRDTVSSCLSWFFWLVSKNPAAGAK 331

Query: 1043 IQEEIKLNLQSNGKESFHKR----EELSKLVYLHGALCETLRLFPPVALEHKAPIQADVL 1210
            I+EE+K  L    KE+  +R    EE+ KLVYLHGALCE+LRL+PP+  E K+P++ D+L
Sbjct: 332  IREELKTTLPE--KEAEKRRLFDIEEIKKLVYLHGALCESLRLYPPIHSEFKSPVRQDIL 389

Query: 1211 PSGHHVNKDSKIVLSFYSMGRMESIWGKDCLDFKPERWITNNGGIKHEPSYKFPAFNAGP 1390
            PSGH VN  +KIV S Y+MGRM S+WG DC +FKPERWIT+ GG+KHEPSYKF +FNAGP
Sbjct: 390  PSGHRVNPKTKIVFSLYAMGRMSSVWGDDCFEFKPERWITDRGGMKHEPSYKFFSFNAGP 449

Query: 1391 RTCLGKEMAFXXXXXXXXXXXXXXXXKVVDGHPIVPGDSIIMHIKHGLKTTISNK 1555
            RTCLGK++AF                +VVD H + P  S+I+H+KHG+K  ++ +
Sbjct: 450  RTCLGKDVAFTQMKAVVAAIIYNYQVQVVDEHTVTPSQSVILHMKHGMKVKLTKR 504


>ref|XP_002321857.1| cytochrome P450 [Populus trichocarpa] gi|222868853|gb|EEF05984.1|
            cytochrome P450 [Populus trichocarpa]
          Length = 506

 Score =  482 bits (1241), Expect = e-133
 Identities = 238/475 (50%), Positives = 318/475 (66%), Gaps = 6/475 (1%)
 Frame = +2

Query: 149  DWPIFRMTPDLIKNGHRLHEFMTEVLKQNHNTFQFKGPALSNLDMLCTCEPADIHYILSK 328
            +WP+  M P L+   HR H+++T+VL+Q+  TFQFKGP   ++D+L T +PA+IHYI S 
Sbjct: 32   NWPLIGMLPGLLFQVHRFHDWVTDVLEQSKCTFQFKGPWFGDMDILVTADPANIHYITSS 91

Query: 329  NFANYPKGPEFRKIFDILGDGIFNADAYLWELHRKNTTMLINQGEFYKMVIDTTTRKVKN 508
            NF+N+ KG +F K FDILGDGI NAD  LW+  RK    L+N   FY+ ++ T   KV+ 
Sbjct: 92   NFSNFEKGSDFNKRFDILGDGIINADRDLWKSQRKVAQALVNHRLFYQRLVKTIQDKVER 151

Query: 509  GLIPVLAYVSKHGIEVDLQELFQRLAFDNICSILLDHDPQSLSISLPYLPCEKAFTATEE 688
            GLIPVL +V K  + +DLQ+LFQR  FD  C I+   DP  LSI LP +   KA     E
Sbjct: 152  GLIPVLEHVYKQSLVLDLQDLFQRFTFDATCVIVTGFDPGCLSIDLPEVAFSKAMDNAME 211

Query: 689  AILYRHILPESIWKLQSLIGFGKEKGLNKARKAFDEFIYRCISMRRQQAVSG-QFSTSGE 865
            AI YRH+LPES W+LQ  +G GKEK L KAR+  D  I   ISM+R++   G +      
Sbjct: 212  AIFYRHVLPESTWRLQRWLGIGKEKKLKKARETLDRIIAEIISMKREELSKGNRLMEEDG 271

Query: 866  DKLDSLTAHM-EAYKERNGNQEKLLRDTLLNLMIAGRDTTSTALSWFFWLLSKNPSVEFK 1042
            + +D LT++M E Y     + ++ LRDT++  M+AGRDT S+ LSWFFWL+SKNP+   K
Sbjct: 272  EGIDLLTSYMSEDYNMGFKSDDEFLRDTIVTFMLAGRDTVSSCLSWFFWLVSKNPAAGAK 331

Query: 1043 IQEEIKLNLQSNGKESFHKR----EELSKLVYLHGALCETLRLFPPVALEHKAPIQADVL 1210
            I+EE+K  L    KE+  +R    EE+ KLVYLHGALCE+LRL+PP+  E K+P++ D+L
Sbjct: 332  IREELKTTLPE--KEAEKRRLFDIEEIKKLVYLHGALCESLRLYPPIHSEFKSPVRQDIL 389

Query: 1211 PSGHHVNKDSKIVLSFYSMGRMESIWGKDCLDFKPERWITNNGGIKHEPSYKFPAFNAGP 1390
            PSGH VN  +KIV S Y+MGRM S+WG DC +FKPERWIT+ GG+KHEPSYKF +FNAGP
Sbjct: 390  PSGHRVNPKAKIVFSLYAMGRMSSVWGDDCFEFKPERWITDRGGMKHEPSYKFFSFNAGP 449

Query: 1391 RTCLGKEMAFXXXXXXXXXXXXXXXXKVVDGHPIVPGDSIIMHIKHGLKTTISNK 1555
            RTCLGK++AF                +VVD H + P  S+I+H+KHG+K  ++ +
Sbjct: 450  RTCLGKDVAFTQMKAVVAAIIYNYQVQVVDEHTVTPSQSVILHMKHGMKVKLTKR 504