BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00013776
         (1860 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002527733.1| GMP synthase, putative [Ricinus communis] gi...   475   e-131
ref|XP_002304500.1| predicted protein [Populus trichocarpa] gi|2...   474   e-131
emb|CBI34663.3| unnamed protein product [Vitis vinifera]              473   e-130
ref|XP_002274590.1| PREDICTED: GMP synthase [glutamine-hydrolyzi...   473   e-130
ref|XP_003525394.1| PREDICTED: GMP synthase [glutamine-hydrolyzi...   471   e-130

>ref|XP_002527733.1| GMP synthase, putative [Ricinus communis]
           gi|223532874|gb|EEF34646.1| GMP synthase, putative
           [Ricinus communis]
          Length = 353

 Score =  475 bits (1222), Expect = e-131
 Identities = 233/247 (94%), Positives = 238/247 (96%)
 Frame = -3

Query: 787 VDAVEQFLSKLKGVVDPEAKRKIIGKEFICIFDAFAHDMEQKTGKKPTYLVQGTLYPDVI 608
           VDA EQFLSKLKGVVDPE KRKIIGKEFI IFDAFAHD+EQK GKKP YLVQGTLYPDVI
Sbjct: 107 VDASEQFLSKLKGVVDPEMKRKIIGKEFISIFDAFAHDLEQKLGKKPAYLVQGTLYPDVI 166

Query: 607 ESCPPPGSGRTHSHTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRELGRILNVPVPFLK 428
           ESCPPPGSGRTHSHTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVR+LGRIL VP  FLK
Sbjct: 167 ESCPPPGSGRTHSHTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRQLGRILYVPEGFLK 226

Query: 427 RHPFPGPGLAVRVLGDVTEGNALDILRQVDEIFIQSIKDAGIYDSIWQAFAVFLPIRSVG 248
           RHPFPGPGLAVRVLGDVTEGNALDILRQVDEIFIQSIKDAG+YD+IWQAFAVFLP+RSVG
Sbjct: 227 RHPFPGPGLAVRVLGDVTEGNALDILRQVDEIFIQSIKDAGLYDTIWQAFAVFLPVRSVG 286

Query: 247 VQGDQRTHSHVVALRAVTSQDGMTADWYNFEHKFLDDVARKICNSVRGVNRVVQDITSKP 68
           VQGDQRTHSHVVALRAVTSQDGMTADWY FEHKFLDDVARKICNSVRGVNRVVQDITSKP
Sbjct: 287 VQGDQRTHSHVVALRAVTSQDGMTADWYYFEHKFLDDVARKICNSVRGVNRVVQDITSKP 346

Query: 67  PSTIEWE 47
           PSTIEWE
Sbjct: 347 PSTIEWE 353



 Score = 99.8 bits (247), Expect = 2e-18
 Identities = 47/60 (78%), Positives = 54/60 (90%)
 Frame = -2

Query: 1121 FLFNVCGVTAGWTMTDVMEEEIKVIKEMVGTEDHVICALSGGVDSTVAATLVHKAIGDRL 942
            FLF+VCGV A W+M +V++EEIK++K  VG EDHVICALSGGVDSTVAATLVHKAIGDRL
Sbjct: 15   FLFDVCGVCANWSMENVVDEEIKMVKGTVGPEDHVICALSGGVDSTVAATLVHKAIGDRL 74


>ref|XP_002304500.1| predicted protein [Populus trichocarpa] gi|222841932|gb|EEE79479.1|
            predicted protein [Populus trichocarpa]
          Length = 534

 Score =  474 bits (1221), Expect = e-131
 Identities = 232/247 (93%), Positives = 238/247 (96%)
 Frame = -3

Query: 787  VDAVEQFLSKLKGVVDPEAKRKIIGKEFICIFDAFAHDMEQKTGKKPTYLVQGTLYPDVI 608
            VDA  +FLSKLKGVVDPE KRKIIGKEFI IFDAFAH++EQK GKKP YLVQGTLYPDVI
Sbjct: 288  VDASNEFLSKLKGVVDPEMKRKIIGKEFISIFDAFAHELEQKLGKKPAYLVQGTLYPDVI 347

Query: 607  ESCPPPGSGRTHSHTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRELGRILNVPVPFLK 428
            ESCPPPGSGRTHSHTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVR+LGRILNVP  FLK
Sbjct: 348  ESCPPPGSGRTHSHTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRQLGRILNVPDAFLK 407

Query: 427  RHPFPGPGLAVRVLGDVTEGNALDILRQVDEIFIQSIKDAGIYDSIWQAFAVFLPIRSVG 248
            RHPFPGPGLAVRVLGDVTEGNALDILRQVDEIFIQSIKDAG+YDSIWQAFAVFLP+RSVG
Sbjct: 408  RHPFPGPGLAVRVLGDVTEGNALDILRQVDEIFIQSIKDAGLYDSIWQAFAVFLPVRSVG 467

Query: 247  VQGDQRTHSHVVALRAVTSQDGMTADWYNFEHKFLDDVARKICNSVRGVNRVVQDITSKP 68
            VQGDQRTHSHVVALRAVTSQDGMTADWY FEHKFLDDVARKICNSVRGVNRVVQDITSKP
Sbjct: 468  VQGDQRTHSHVVALRAVTSQDGMTADWYYFEHKFLDDVARKICNSVRGVNRVVQDITSKP 527

Query: 67   PSTIEWE 47
            PSTIEWE
Sbjct: 528  PSTIEWE 534



 Score =  245 bits (626), Expect = 3e-62
 Identities = 117/170 (68%), Positives = 148/170 (87%)
 Frame = -1

Query: 1761 MEAQAVKSNLVLILDFGSQYTHLITRRIRSLSVFSLCISGTSSLKSITDLNPSVIILSGG 1582
            M+ +AVKS+LVLILD+GSQYTHLITRRIRSL++FSLCISGTSSL++IT  NP V+ILSGG
Sbjct: 1    MDPKAVKSDLVLILDYGSQYTHLITRRIRSLNIFSLCISGTSSLETITSHNPKVVILSGG 60

Query: 1581 PHSVHATDAPSFPDGFVEYVERNGVFVLGICYGLHLLVEKLGGEVRVGEKQEYGRMEIEV 1402
            PHSVH+ ++P+FP GFVE+ ++ G+FVLG+CYGL L+V++LGG+V VG++QEYGRMEIEV
Sbjct: 61   PHSVHSANSPTFPSGFVEWAQKGGIFVLGVCYGLQLIVQRLGGQVDVGQRQEYGRMEIEV 120

Query: 1401 LEETDLFDCDKSGGRRQIVWMSHGDEAVRLPEGFRVVARSQQGSVAAVEN 1252
             +   +F  +K  G +Q+VWMSHGDE V+LP GF VVARSQQG+VAAVEN
Sbjct: 121  EKNLGVFG-NKKVGDKQVVWMSHGDETVKLPYGFEVVARSQQGAVAAVEN 169



 Score = 99.4 bits (246), Expect = 3e-18
 Identities = 46/60 (76%), Positives = 54/60 (90%)
 Frame = -2

Query: 1121 FLFNVCGVTAGWTMTDVMEEEIKVIKEMVGTEDHVICALSGGVDSTVAATLVHKAIGDRL 942
            FLF+VCGV+ GW M +V++EEI+VI + VG E+HVICALSGGVDSTVAATLVHKAIGDRL
Sbjct: 196  FLFDVCGVSPGWNMENVLDEEIRVINDAVGPEEHVICALSGGVDSTVAATLVHKAIGDRL 255


>emb|CBI34663.3| unnamed protein product [Vitis vinifera]
          Length = 493

 Score =  473 bits (1216), Expect = e-130
 Identities = 233/247 (94%), Positives = 235/247 (95%)
 Frame = -3

Query: 787 VDAVEQFLSKLKGVVDPEAKRKIIGKEFICIFDAFAHDMEQKTGKKPTYLVQGTLYPDVI 608
           VDA  QFLSKLKGVVDPE KRKIIGKEFI  FDAF  D+E K GKKP YLVQGTLYPDVI
Sbjct: 247 VDATNQFLSKLKGVVDPEMKRKIIGKEFISSFDAFGQDLEHKFGKKPVYLVQGTLYPDVI 306

Query: 607 ESCPPPGSGRTHSHTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRELGRILNVPVPFLK 428
           ESCPPPGSGRTHSHTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRELGRILNVP  FL+
Sbjct: 307 ESCPPPGSGRTHSHTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRELGRILNVPRAFLQ 366

Query: 427 RHPFPGPGLAVRVLGDVTEGNALDILRQVDEIFIQSIKDAGIYDSIWQAFAVFLPIRSVG 248
           RHPFPGPGLAVRVLGDVTEGNALDILRQVDEIFIQSIKDAGIYDSIWQAFAVFLPIRSVG
Sbjct: 367 RHPFPGPGLAVRVLGDVTEGNALDILRQVDEIFIQSIKDAGIYDSIWQAFAVFLPIRSVG 426

Query: 247 VQGDQRTHSHVVALRAVTSQDGMTADWYNFEHKFLDDVARKICNSVRGVNRVVQDITSKP 68
           VQGDQRTHSHVVALRAVTSQDGMTADWYNFEHKFLDDVARKICNSVRGVNRVVQDITSKP
Sbjct: 427 VQGDQRTHSHVVALRAVTSQDGMTADWYNFEHKFLDDVARKICNSVRGVNRVVQDITSKP 486

Query: 67  PSTIEWE 47
           PSTIEWE
Sbjct: 487 PSTIEWE 493



 Score =  179 bits (453), Expect = 3e-42
 Identities = 100/170 (58%), Positives = 111/170 (65%)
 Frame = -1

Query: 1761 MEAQAVKSNLVLILDFGSQYTHLITRRIRSLSVFSLCISGTSSLKSITDLNPSVIILSGG 1582
            M+ + VKSNLVLILD+GSQYTHLITRRIRSLSVFSLCISGTSSLKSI DL P VIILSGG
Sbjct: 1    MDPENVKSNLVLILDYGSQYTHLITRRIRSLSVFSLCISGTSSLKSIVDLKPRVIILSGG 60

Query: 1581 PHSVHATDAPSFPDGFVEYVERNGVFVLGICYGLHLLVEKLGGEVRVGEKQEYGRMEIEV 1402
            PHSVH+ ++PSFPDGF+E V   G+F                           G  E+  
Sbjct: 61   PHSVHSPNSPSFPDGFIEVVRACGLF---------------------------GSKEV-- 91

Query: 1401 LEETDLFDCDKSGGRRQIVWMSHGDEAVRLPEGFRVVARSQQGSVAAVEN 1252
                         G +Q VWMSHGDEA  LPEGF VVARSQQG+VAAVEN
Sbjct: 92   -------------GHKQTVWMSHGDEAAELPEGFEVVARSQQGAVAAVEN 128



 Score = 99.4 bits (246), Expect = 3e-18
 Identities = 48/60 (80%), Positives = 52/60 (86%)
 Frame = -2

Query: 1121 FLFNVCGVTAGWTMTDVMEEEIKVIKEMVGTEDHVICALSGGVDSTVAATLVHKAIGDRL 942
            FLF+VC V AGW M DV+ EEIK+IK MV  +DHVICALSGGVDSTVAATLVHKAIGDRL
Sbjct: 155  FLFDVCRVDAGWNMEDVLNEEIKLIKGMVAPDDHVICALSGGVDSTVAATLVHKAIGDRL 214


>ref|XP_002274590.1| PREDICTED: GMP synthase [glutamine-hydrolyzing] [Vitis vinifera]
          Length = 534

 Score =  473 bits (1216), Expect = e-130
 Identities = 233/247 (94%), Positives = 235/247 (95%)
 Frame = -3

Query: 787  VDAVEQFLSKLKGVVDPEAKRKIIGKEFICIFDAFAHDMEQKTGKKPTYLVQGTLYPDVI 608
            VDA  QFLSKLKGVVDPE KRKIIGKEFI  FDAF  D+E K GKKP YLVQGTLYPDVI
Sbjct: 288  VDATNQFLSKLKGVVDPEMKRKIIGKEFISSFDAFGQDLEHKFGKKPVYLVQGTLYPDVI 347

Query: 607  ESCPPPGSGRTHSHTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRELGRILNVPVPFLK 428
            ESCPPPGSGRTHSHTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRELGRILNVP  FL+
Sbjct: 348  ESCPPPGSGRTHSHTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRELGRILNVPRAFLQ 407

Query: 427  RHPFPGPGLAVRVLGDVTEGNALDILRQVDEIFIQSIKDAGIYDSIWQAFAVFLPIRSVG 248
            RHPFPGPGLAVRVLGDVTEGNALDILRQVDEIFIQSIKDAGIYDSIWQAFAVFLPIRSVG
Sbjct: 408  RHPFPGPGLAVRVLGDVTEGNALDILRQVDEIFIQSIKDAGIYDSIWQAFAVFLPIRSVG 467

Query: 247  VQGDQRTHSHVVALRAVTSQDGMTADWYNFEHKFLDDVARKICNSVRGVNRVVQDITSKP 68
            VQGDQRTHSHVVALRAVTSQDGMTADWYNFEHKFLDDVARKICNSVRGVNRVVQDITSKP
Sbjct: 468  VQGDQRTHSHVVALRAVTSQDGMTADWYNFEHKFLDDVARKICNSVRGVNRVVQDITSKP 527

Query: 67   PSTIEWE 47
            PSTIEWE
Sbjct: 528  PSTIEWE 534



 Score =  264 bits (675), Expect = 5e-68
 Identities = 134/170 (78%), Positives = 147/170 (86%)
 Frame = -1

Query: 1761 MEAQAVKSNLVLILDFGSQYTHLITRRIRSLSVFSLCISGTSSLKSITDLNPSVIILSGG 1582
            M+ + VKSNLVLILD+GSQYTHLITRRIRSLSVFSLCISGTSSLKSI DL P VIILSGG
Sbjct: 1    MDPENVKSNLVLILDYGSQYTHLITRRIRSLSVFSLCISGTSSLKSIVDLKPRVIILSGG 60

Query: 1581 PHSVHATDAPSFPDGFVEYVERNGVFVLGICYGLHLLVEKLGGEVRVGEKQEYGRMEIEV 1402
            PHSVH+ ++PSFPDGFV+YVE NGVFVLGICYGL LLV++LGG VRVGE+QEYGRMEIEV
Sbjct: 61   PHSVHSPNSPSFPDGFVDYVESNGVFVLGICYGLQLLVQRLGGIVRVGERQEYGRMEIEV 120

Query: 1401 LEETDLFDCDKSGGRRQIVWMSHGDEAVRLPEGFRVVARSQQGSVAAVEN 1252
            +    LF   K  G +Q VWMSHGDEA  LPEGF VVARSQQG+VAAVEN
Sbjct: 121  VRACGLFG-SKEVGHKQTVWMSHGDEAAELPEGFEVVARSQQGAVAAVEN 169



 Score = 99.4 bits (246), Expect = 3e-18
 Identities = 48/60 (80%), Positives = 52/60 (86%)
 Frame = -2

Query: 1121 FLFNVCGVTAGWTMTDVMEEEIKVIKEMVGTEDHVICALSGGVDSTVAATLVHKAIGDRL 942
            FLF+VC V AGW M DV+ EEIK+IK MV  +DHVICALSGGVDSTVAATLVHKAIGDRL
Sbjct: 196  FLFDVCRVDAGWNMEDVLNEEIKLIKGMVAPDDHVICALSGGVDSTVAATLVHKAIGDRL 255


>ref|XP_003525394.1| PREDICTED: GMP synthase [glutamine-hydrolyzing]-like [Glycine max]
          Length = 534

 Score =  471 bits (1213), Expect = e-130
 Identities = 228/247 (92%), Positives = 239/247 (96%)
 Frame = -3

Query: 787  VDAVEQFLSKLKGVVDPEAKRKIIGKEFICIFDAFAHDMEQKTGKKPTYLVQGTLYPDVI 608
            VDAV QFL+KLKGVVDPE KRKIIGKEFICIFDAFA ++E K GKKP+YLVQGTLYPDVI
Sbjct: 288  VDAVNQFLTKLKGVVDPETKRKIIGKEFICIFDAFAQELEGKLGKKPSYLVQGTLYPDVI 347

Query: 607  ESCPPPGSGRTHSHTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRELGRILNVPVPFLK 428
            ESCPPPG+GRTHSHTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVR+LGRIL+VPV FL+
Sbjct: 348  ESCPPPGTGRTHSHTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRQLGRILDVPVAFLR 407

Query: 427  RHPFPGPGLAVRVLGDVTEGNALDILRQVDEIFIQSIKDAGIYDSIWQAFAVFLPIRSVG 248
            RHPFPGPGLAVRVLGDVTEGNALDILRQVDEIFIQSIKDAG+YDSIWQAFAVFLP+RSVG
Sbjct: 408  RHPFPGPGLAVRVLGDVTEGNALDILRQVDEIFIQSIKDAGLYDSIWQAFAVFLPVRSVG 467

Query: 247  VQGDQRTHSHVVALRAVTSQDGMTADWYNFEHKFLDDVARKICNSVRGVNRVVQDITSKP 68
            VQGDQRTHSHVVALRAVTSQDGMTADWY FEHKFLDDV+RKICN VRGVNRVVQDITSKP
Sbjct: 468  VQGDQRTHSHVVALRAVTSQDGMTADWYYFEHKFLDDVSRKICNGVRGVNRVVQDITSKP 527

Query: 67   PSTIEWE 47
            PSTIEWE
Sbjct: 528  PSTIEWE 534



 Score =  255 bits (651), Expect(2) = 2e-91
 Identities = 126/171 (73%), Positives = 149/171 (87%)
 Frame = -1

Query: 1761 MEAQAVKSNLVLILDFGSQYTHLITRRIRSLSVFSLCISGTSSLKSITDLNPSVIILSGG 1582
            M+ + VKS+LVLILD+GSQYTHLITRRIRSLSVFSLCISGTSSL +I DLNPSV+ILSGG
Sbjct: 1    MDPKNVKSDLVLILDYGSQYTHLITRRIRSLSVFSLCISGTSSLSTIADLNPSVVILSGG 60

Query: 1581 PHSVHATDAPSFPDGFVEYVERNGVFVLGICYGLHLLVEKLGGEVRVGEKQEYGRMEIEV 1402
            PHSVH  D+PSFPDGF+++ + NGV VLGICYGL LLV++LGG+VRVG+KQEYGRMEI  
Sbjct: 61   PHSVHTPDSPSFPDGFLQWAQSNGVTVLGICYGLQLLVQRLGGDVRVGDKQEYGRMEICA 120

Query: 1401 LEETDLFDCDKSGGRRQIVWMSHGDEAVRLPEGFRVVARSQQGSVAAVENP 1249
             + + LF  +K  G+RQ+VWMSHGDEAV LP+GF VVARS QG+VAA+ENP
Sbjct: 121  DKPSALFGPEKV-GKRQVVWMSHGDEAVALPDGFHVVARSDQGAVAAIENP 170



 Score =  109 bits (273), Expect(2) = 2e-91
 Identities = 53/62 (85%), Positives = 57/62 (91%)
 Frame = -2

Query: 1127 KRFLFNVCGVTAGWTMTDVMEEEIKVIKEMVGTEDHVICALSGGVDSTVAATLVHKAIGD 948
            +RFLF+VCGV AGW M DVMEEEIKVIKE VG ++HVICALSGGVDSTVAATLVHKAIGD
Sbjct: 194  RRFLFDVCGVGAGWKMEDVMEEEIKVIKETVGPDEHVICALSGGVDSTVAATLVHKAIGD 253

Query: 947  RL 942
            RL
Sbjct: 254  RL 255