BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00013760
         (1288 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002510288.1| ent-kaurene oxidase, putative [Ricinus commu...   492   e-137
gb|AEF32084.1| ent-kaurene oxidase [Castanea mollissima]              489   e-136
dbj|BAG71198.1| ent-kaurene oxidase 2 [Lactuca sativa]                486   e-135
dbj|BAG71197.1| ent-kaurene oxidase 1 [Lactuca sativa]                479   e-133
ref|XP_004141236.1| PREDICTED: ent-kaurene oxidase, chloroplasti...   474   e-131

>ref|XP_002510288.1| ent-kaurene oxidase, putative [Ricinus communis]
            gi|223550989|gb|EEF52475.1| ent-kaurene oxidase, putative
            [Ricinus communis]
          Length = 513

 Score =  492 bits (1267), Expect = e-137
 Identities = 238/377 (63%), Positives = 301/377 (79%)
 Frame = -1

Query: 1132 SLWLYKRLMGHKGKSHXXXXXXXXXXXXXLFGNLLQLKEKKPHKTFTKWAETYGPIYSIR 953
            S+++ K+L+ ++ K H             + GNLLQLKEKKPHKTF +WAETYGPIYSIR
Sbjct: 29   SMFVMKKLISNQKKRHARLPPVPEVPGWPVIGNLLQLKEKKPHKTFMRWAETYGPIYSIR 88

Query: 952  TGSTTMVVLNSNDVVKEAMVTRFPAISTRKLSQALKILTSDKSMVAVSDYDEFHKTVKRH 773
            TG++T++VLNS DV KEAMVTR+ +ISTRKLS+AL ILT DK MVA SDYDEFHK VKR+
Sbjct: 89   TGASTIIVLNSTDVAKEAMVTRYSSISTRKLSKALVILTQDKCMVATSDYDEFHKMVKRY 148

Query: 772  LLTHILGPNAQKRQRIHRDALIENISDQLHDLSKKYPLEAINFRELFESGLFGLAMKQTL 593
            LLT++LG NAQ+R R HRD LI+NIS + H   K  P + +NFR++FES LFGL++K+ L
Sbjct: 149  LLTNVLGANAQRRHRCHRDTLIKNISSRFHAHVKTNPEQTVNFRDIFESELFGLSLKEAL 208

Query: 592  GEDVESIYVDELGTTMSRQDIFNCLVHDPMAGAIEVDWRDFFPYLKWIPNRSFENKIEQM 413
            G+DV+SIYVDELGTT+SR++IF  LV DPM GAI+VDWRDFFPYL+W+PN+S+E KI+QM
Sbjct: 209  GKDVQSIYVDELGTTLSRKEIFEVLVLDPMEGAIDVDWRDFFPYLRWVPNKSWEMKIQQM 268

Query: 412  DIRRMAVMKFLVENARKQSASREGMQCYLDYLESEGKGLSEKQIQMLLWEVIIETSDTTV 233
              RR AVM  L++  +K+ A  E + CYLD+L SE   L+++QI+ML+WE I+ETSDTT+
Sbjct: 269  HFRRKAVMGALIKEQKKRIALGEEVNCYLDFLSSEST-LTDEQIRMLIWETIVETSDTTM 327

Query: 232  VATEWALYELAKDSKRQDRLYEEISRVCGSDKITEEKLPQLPYLYAIFQETIRVYSPAPI 53
            VATEWA+YELAK+   QD LY +I  VCGS+KITEE L +LPYL A+F ETIR YSPAP+
Sbjct: 328  VATEWAIYELAKNPNCQDLLYRQIQNVCGSEKITEEHLSKLPYLNAVFHETIRKYSPAPV 387

Query: 52   VPLRYVHEDTELGGYFV 2
            +PLRYVHEDTELGGY++
Sbjct: 388  IPLRYVHEDTELGGYYI 404


>gb|AEF32084.1| ent-kaurene oxidase [Castanea mollissima]
          Length = 514

 Score =  489 bits (1260), Expect = e-136
 Identities = 235/346 (67%), Positives = 291/346 (84%)
 Frame = -1

Query: 1039 GNLLQLKEKKPHKTFTKWAETYGPIYSIRTGSTTMVVLNSNDVVKEAMVTRFPAISTRKL 860
            GNLLQLKEKKP+KTF +WAE +GPIYSIRTG++TMVV+NS  V KEAMVTRF +ISTRKL
Sbjct: 61   GNLLQLKEKKPYKTFLRWAEIHGPIYSIRTGASTMVVVNSTHVAKEAMVTRFSSISTRKL 120

Query: 859  SQALKILTSDKSMVAVSDYDEFHKTVKRHLLTHILGPNAQKRQRIHRDALIENISDQLHD 680
            S+AL++LTS+KSMVA SDY+EFHK VK+++L  +LG NAQKR RIHRD LIEN+ ++LH 
Sbjct: 121  SKALELLTSNKSMVATSDYNEFHKMVKKYILAELLGANAQKRHRIHRDTLIENVLNKLHA 180

Query: 679  LSKKYPLEAINFRELFESGLFGLAMKQTLGEDVESIYVDELGTTMSRQDIFNCLVHDPMA 500
             +K  PL+A+NFR++FES LFGLAMKQ LG DV+S++V+ELGTT+SR++I+N LV D + 
Sbjct: 181  HTKNSPLQAVNFRKIFESELFGLAMKQALGYDVDSLFVEELGTTLSREEIYNVLVSDMLK 240

Query: 499  GAIEVDWRDFFPYLKWIPNRSFENKIEQMDIRRMAVMKFLVENARKQSASREGMQCYLDY 320
            GAIEVDWRDFFPYLKWIPN+SFE KI+++  RR AVM  +V+  +K  AS +G  CYL+Y
Sbjct: 241  GAIEVDWRDFFPYLKWIPNKSFEMKIQRLASRRQAVMNSIVKEQKKSIASGKGENCYLNY 300

Query: 319  LESEGKGLSEKQIQMLLWEVIIETSDTTVVATEWALYELAKDSKRQDRLYEEISRVCGSD 140
            L SE K L+EKQI +L WE IIET+DTTVV TEWA+YELAK+ K+QDRLY EI  VCG+D
Sbjct: 301  LLSEAKTLTEKQISILAWETIIETADTTVVTTEWAMYELAKNPKQQDRLYNEIQNVCGTD 360

Query: 139  KITEEKLPQLPYLYAIFQETIRVYSPAPIVPLRYVHEDTELGGYFV 2
            KITEE L +LPYL A+F ET+R YSP+P+VPLRY HEDT+LGGY+V
Sbjct: 361  KITEEHLSKLPYLSAVFHETLRKYSPSPLVPLRYAHEDTQLGGYYV 406


>dbj|BAG71198.1| ent-kaurene oxidase 2 [Lactuca sativa]
          Length = 512

 Score =  486 bits (1251), Expect = e-135
 Identities = 242/408 (59%), Positives = 304/408 (74%), Gaps = 2/408 (0%)
 Frame = -1

Query: 1219 MDVVVNLQSTPMXXXXXXXXXXXXXXXGISLWLYKRLMGHKGKSHXXXXXXXXXXXXXL- 1043
            MD V+++Q+ P+                +  W    L  +   SH             + 
Sbjct: 1    MDGVIDMQTIPLRTAIAIGGTAVALVVALYFWF---LRSYASPSHHSNHLPPVPEVPGVP 57

Query: 1042 -FGNLLQLKEKKPHKTFTKWAETYGPIYSIRTGSTTMVVLNSNDVVKEAMVTRFPAISTR 866
              GNLLQLKEKKP+ TFTKWAE YGPIYSIRTG+T+MVV++SN++ KE +VTRFP+ISTR
Sbjct: 58   VLGNLLQLKEKKPYMTFTKWAEMYGPIYSIRTGATSMVVVSSNEIAKEVVVTRFPSISTR 117

Query: 865  KLSQALKILTSDKSMVAVSDYDEFHKTVKRHLLTHILGPNAQKRQRIHRDALIENISDQL 686
            KLS ALK+LT DKSMVA+SDY ++HKTVKRH+LT +LGPNAQK+ R HRD ++EN+S++L
Sbjct: 118  KLSYALKVLTEDKSMVAMSDYHDYHKTVKRHILTAVLGPNAQKKFRAHRDTMMENVSNEL 177

Query: 685  HDLSKKYPLEAINFRELFESGLFGLAMKQTLGEDVESIYVDELGTTMSRQDIFNCLVHDP 506
            H   +K P + +N R++F+S LFGLAMKQ LG+DVESIYV +L TTM R++IF  LV DP
Sbjct: 178  HAFFEKNPNQEVNLRKIFQSQLFGLAMKQALGKDVESIYVKDLETTMKREEIFEVLVVDP 237

Query: 505  MAGAIEVDWRDFFPYLKWIPNRSFENKIEQMDIRRMAVMKFLVENARKQSASREGMQCYL 326
            M GAIEVDWRDFFPYLKW+PN+SFEN I +M  RR AVMK L++  +K+ AS E +  Y+
Sbjct: 238  MMGAIEVDWRDFFPYLKWVPNKSFENIIHRMYTRREAVMKALIQEHKKRIASGENLNSYI 297

Query: 325  DYLESEGKGLSEKQIQMLLWEVIIETSDTTVVATEWALYELAKDSKRQDRLYEEISRVCG 146
            DYL SE + L++KQ+ M LWE IIE+SDTT+V TEWA+YELAK+   QDRLYEEI  VCG
Sbjct: 298  DYLLSEAQTLTDKQLLMSLWEPIIESSDTTMVTTEWAMYELAKNPNMQDRLYEEIQSVCG 357

Query: 145  SDKITEEKLPQLPYLYAIFQETIRVYSPAPIVPLRYVHEDTELGGYFV 2
            S+KITEE L QLPYLYA+FQET+R + P PI+PLRYVHE+T LGGY V
Sbjct: 358  SEKITEENLSQLPYLYAVFQETLRKHCPVPIMPLRYVHENTVLGGYHV 405


>dbj|BAG71197.1| ent-kaurene oxidase 1 [Lactuca sativa]
          Length = 511

 Score =  479 bits (1232), Expect = e-133
 Identities = 232/346 (67%), Positives = 287/346 (82%)
 Frame = -1

Query: 1039 GNLLQLKEKKPHKTFTKWAETYGPIYSIRTGSTTMVVLNSNDVVKEAMVTRFPAISTRKL 860
            GNLL+LKEKKP+KTFTKWAETYGPIYSI+TG+T+MVV+NSN + KEAMVTRF +ISTRKL
Sbjct: 59   GNLLELKEKKPYKTFTKWAETYGPIYSIKTGATSMVVVNSNQLAKEAMVTRFDSISTRKL 118

Query: 859  SQALKILTSDKSMVAVSDYDEFHKTVKRHLLTHILGPNAQKRQRIHRDALIENISDQLHD 680
            S+AL+ILT+DK+MVA+SDYD++HKTVKR+LLT ILGP AQKR R HRDA+ +N+S QLH 
Sbjct: 119  SKALQILTADKTMVAMSDYDDYHKTVKRNLLTSILGPAAQKRHRAHRDAMGDNLSRQLHA 178

Query: 679  LSKKYPLEAINFRELFESGLFGLAMKQTLGEDVESIYVDELGTTMSRQDIFNCLVHDPMA 500
            L+   P EAINFR++F+S LF LA KQT G D+ESI+V +LGTTM+R+++F  LV DPM 
Sbjct: 179  LALNSPQEAINFRQIFQSELFTLAFKQTFGRDIESIFVGDLGTTMTREEMFQILVVDPMM 238

Query: 499  GAIEVDWRDFFPYLKWIPNRSFENKIEQMDIRRMAVMKFLVENARKQSASREGMQCYLDY 320
            GAI+VDWRDFFPYLKWIPN   E KIEQM IRR AVMK +++  RK+  S E +  Y+D+
Sbjct: 239  GAIDVDWRDFFPYLKWIPNAKLEEKIEQMYIRRKAVMKAVIQEHRKRIDSGENLDSYIDF 298

Query: 319  LESEGKGLSEKQIQMLLWEVIIETSDTTVVATEWALYELAKDSKRQDRLYEEISRVCGSD 140
            L +E + L+EKQ+ M LWE IIETSDTT+V TEWA+YEL+K   +Q RLY EI  +CGS+
Sbjct: 299  LLAEAQPLTEKQLLMSLWEPIIETSDTTMVTTEWAMYELSKHPNKQQRLYNEIRNICGSE 358

Query: 139  KITEEKLPQLPYLYAIFQETIRVYSPAPIVPLRYVHEDTELGGYFV 2
            KITEEKL ++PYL A+F ET+RV+SP  I+PLRYVHE+TELGGY V
Sbjct: 359  KITEEKLCKMPYLSAVFHETLRVHSPVSIIPLRYVHENTELGGYHV 404


>ref|XP_004141236.1| PREDICTED: ent-kaurene oxidase, chloroplastic-like [Cucumis sativus]
            gi|449498669|ref|XP_004160600.1| PREDICTED: ent-kaurene
            oxidase, chloroplastic-like [Cucumis sativus]
            gi|383290047|gb|AFH03045.1| ent-kaurene oxidase [Cucumis
            sativus] gi|402169625|gb|AFQ32799.1| ent-kaurene oxidase
            [Cucumis sativus]
          Length = 519

 Score =  474 bits (1219), Expect = e-131
 Identities = 227/346 (65%), Positives = 282/346 (81%)
 Frame = -1

Query: 1039 GNLLQLKEKKPHKTFTKWAETYGPIYSIRTGSTTMVVLNSNDVVKEAMVTRFPAISTRKL 860
            GNLLQLKEKKPHKTF +WAETYG +YSIRTG++T++VLN+ +V KEAMVTR+ +IS+RKL
Sbjct: 65   GNLLQLKEKKPHKTFARWAETYGAVYSIRTGASTVIVLNTTEVAKEAMVTRYGSISSRKL 124

Query: 859  SQALKILTSDKSMVAVSDYDEFHKTVKRHLLTHILGPNAQKRQRIHRDALIENISDQLHD 680
            S+AL ILT+DK MVA+SDY+EFHK VKR++L ++LG NAQK+ R  RDA+IENIS +L  
Sbjct: 125  SKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGANAQKKHRQRRDAMIENISRELFA 184

Query: 679  LSKKYPLEAINFRELFESGLFGLAMKQTLGEDVESIYVDELGTTMSRQDIFNCLVHDPMA 500
              K++PL+ +NFR++FE+ LF LA+K+TLG+D+ESIYVD LGTT+ R+D+F  LV DPM 
Sbjct: 185  HVKEFPLDTVNFRKIFEAELFRLALKETLGKDIESIYVDGLGTTLPREDLFRILVIDPME 244

Query: 499  GAIEVDWRDFFPYLKWIPNRSFENKIEQMDIRRMAVMKFLVENARKQSASREGMQCYLDY 320
            GAIEVDWRDFFPYL+WIPN+  ENKI  MD RR   MK L+E  +K+ A+ E   CY D+
Sbjct: 245  GAIEVDWRDFFPYLRWIPNKRVENKIRNMDFRRRMTMKKLMEEPKKRIAAGEETYCYADF 304

Query: 319  LESEGKGLSEKQIQMLLWEVIIETSDTTVVATEWALYELAKDSKRQDRLYEEISRVCGSD 140
            L SE K L+E QI MLLWE IIETSDTT+V TEWA+YEL+KD +RQD LY++I  VCGS 
Sbjct: 305  LLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELSKDPRRQDYLYQQIQSVCGSA 364

Query: 139  KITEEKLPQLPYLYAIFQETIRVYSPAPIVPLRYVHEDTELGGYFV 2
             +TEE L QLPYL AIF ET+R +SP P+VPLRY HEDT+LGGYFV
Sbjct: 365  TLTEENLSQLPYLTAIFHETLRKHSPVPVVPLRYAHEDTQLGGYFV 410