BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00013745
         (1244 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAC65128.2| short chain alcohol dehydrogenase-like protein [...   434   e-119
ref|XP_002311182.1| predicted protein [Populus trichocarpa] gi|2...   394   e-107
gb|AFK35677.1| unknown [Medicago truncatula]                          388   e-105
ref|NP_196225.1| tropine dehydrogenase [Arabidopsis thaliana] gi...   385   e-104
ref|XP_002277835.1| PREDICTED: tropinone reductase homolog At1g0...   384   e-104

>dbj|BAC65128.2| short chain alcohol dehydrogenase-like protein [Daucus carota]
          Length = 302

 Score =  434 bits (1115), Expect = e-119
 Identities = 214/270 (79%), Positives = 236/270 (87%), Gaps = 1/270 (0%)
 Frame = +3

Query: 21  SRSLPTQISRKSRWSLAGMTALVTGGTRGIGHAVVEELAELGAFVYTCSRNEQELNQRLE 200
           S S    ++R SRWSLAGMTALVTGGTRGIGHA+VEELAELGA VYTCSRNEQELN+RLE
Sbjct: 33  SSSTKAPVTRNSRWSLAGMTALVTGGTRGIGHAIVEELAELGACVYTCSRNEQELNERLE 92

Query: 201 EWRVKGFNVDGSVCDASNKTHRDQLFQRVSSTFNGKLNILVNNVGTNIRKGTAEYTAEEY 380
           EWR KGF+V GSVCD S+ T R+QLFQR+SS F GKL+IL+NNVGTNIR+ T  YTAE+Y
Sbjct: 93  EWRAKGFDVTGSVCDVSSVTEREQLFQRISSCFGGKLHILINNVGTNIRRATENYTAEQY 152

Query: 381 STIMATNLESSFHVCQLAYPLLKTSGSGCIVFISSVAGLVHIGS-GSVYGATKGAINQLT 557
           S +MATNLE+ +H CQLAYPLLK SGSGCIVF SSVAGLVH+G+ GSVYGA KGAINQLT
Sbjct: 153 SIVMATNLEAPYHACQLAYPLLKASGSGCIVFNSSVAGLVHLGTPGSVYGAAKGAINQLT 212

Query: 558 KNLACEWAKDNIRTNCVAPWYIKTSLVEHLLENKEFLDKVVARTPLRRPGEAEEVSSLVA 737
           KNLACEWAKDNIRTNCVAP YIKT  VE L E K FLD++V+RTPLRRPGE EEVSSLVA
Sbjct: 213 KNLACEWAKDNIRTNCVAPGYIKTPPVEKLFERKNFLDRLVSRTPLRRPGETEEVSSLVA 272

Query: 738 YLCLPAASYITGQIIAVDGGFTVNSFERVV 827
           YLC+PAASYITGQIIA+DGG TVN FERVV
Sbjct: 273 YLCMPAASYITGQIIAIDGGLTVNCFERVV 302


>ref|XP_002311182.1| predicted protein [Populus trichocarpa] gi|222851002|gb|EEE88549.1|
           predicted protein [Populus trichocarpa]
          Length = 266

 Score =  394 bits (1011), Expect = e-107
 Identities = 188/259 (72%), Positives = 227/259 (87%)
 Frame = +3

Query: 48  RKSRWSLAGMTALVTGGTRGIGHAVVEELAELGAFVYTCSRNEQELNQRLEEWRVKGFNV 227
           + SRWSL GMTALVTGGTRGIG+A VEELA  GA V+TCSRNE+ELN+ L+EW  KGF V
Sbjct: 8   KDSRWSLHGMTALVTGGTRGIGNATVEELAGFGARVHTCSRNEEELNKCLKEWEAKGFVV 67

Query: 228 DGSVCDASNKTHRDQLFQRVSSTFNGKLNILVNNVGTNIRKGTAEYTAEEYSTIMATNLE 407
            GSVCDAS++  R++L + V S F+GKLNILVNNVGTNIRK T  Y+AEE+S ++ATN E
Sbjct: 68  TGSVCDASSRVQREKLIEEVGSVFHGKLNILVNNVGTNIRKPTTGYSAEEFSNLLATNFE 127

Query: 408 SSFHVCQLAYPLLKTSGSGCIVFISSVAGLVHIGSGSVYGATKGAINQLTKNLACEWAKD 587
           S++H+ Q+A+PLLK SG+G +VFISSVAGL+HIGSGS+YGA+KGAINQLTKNLACEWAKD
Sbjct: 128 SAYHLSQIAHPLLKESGAGSVVFISSVAGLLHIGSGSIYGASKGAINQLTKNLACEWAKD 187

Query: 588 NIRTNCVAPWYIKTSLVEHLLENKEFLDKVVARTPLRRPGEAEEVSSLVAYLCLPAASYI 767
           NIRTNCVAPWYI+TSLVEHLL++K FLDK+++RTPL+R G+ +EVSSLV +LCLPAA+YI
Sbjct: 188 NIRTNCVAPWYIRTSLVEHLLDDKVFLDKIISRTPLQRVGDPKEVSSLVGFLCLPAAAYI 247

Query: 768 TGQIIAVDGGFTVNSFERV 824
           TGQ+I+VDGGFTVN F  V
Sbjct: 248 TGQVISVDGGFTVNGFNPV 266


>gb|AFK35677.1| unknown [Medicago truncatula]
          Length = 271

 Score =  388 bits (997), Expect = e-105
 Identities = 182/258 (70%), Positives = 221/258 (85%)
 Frame = +3

Query: 45  SRKSRWSLAGMTALVTGGTRGIGHAVVEELAELGAFVYTCSRNEQELNQRLEEWRVKGFN 224
           SR SRWSL G TALVTGGTRGIGHA+VEELAE  A VYTCSRN++ELN+RL EW+ KGF+
Sbjct: 8   SRSSRWSLKGFTALVTGGTRGIGHAIVEELAEFCATVYTCSRNQEELNKRLNEWKEKGFS 67

Query: 225 VDGSVCDASNKTHRDQLFQRVSSTFNGKLNILVNNVGTNIRKGTAEYTAEEYSTIMATNL 404
           V GSVCDAS+ + R++L Q V+S+FNGKLNI VNN GTN+RK T EYTAE+YS +M TNL
Sbjct: 68  VYGSVCDASSSSQREELIQNVASSFNGKLNIFVNNAGTNVRKPTIEYTAEDYSKVMTTNL 127

Query: 405 ESSFHVCQLAYPLLKTSGSGCIVFISSVAGLVHIGSGSVYGATKGAINQLTKNLACEWAK 584
           +S++H+CQL YPLLK SG+G IVFISSV  L  +G+GS+Y A+K AINQLTK+LACEWAK
Sbjct: 128 DSAYHLCQLTYPLLKESGNGSIVFISSVGSLTSVGTGSIYAASKAAINQLTKSLACEWAK 187

Query: 585 DNIRTNCVAPWYIKTSLVEHLLENKEFLDKVVARTPLRRPGEAEEVSSLVAYLCLPAASY 764
           DNIR+NCVAPWY KT LVEHL+ N+EF+++V++RTP++R  E  EVSSLV +LCLPAASY
Sbjct: 188 DNIRSNCVAPWYTKTPLVEHLIANEEFVNQVLSRTPIKRIAETHEVSSLVTFLCLPAASY 247

Query: 765 ITGQIIAVDGGFTVNSFE 818
           ITGQI++VDGGFTVN F+
Sbjct: 248 ITGQIVSVDGGFTVNGFQ 265


>ref|NP_196225.1| tropine dehydrogenase [Arabidopsis thaliana]
           gi|8978342|dbj|BAA98195.1| short chain alcohol
           dehydrogenase-like [Arabidopsis thaliana]
           gi|27754526|gb|AAO22710.1| putative short chain alcohol
           dehydrogenase [Arabidopsis thaliana]
           gi|28394081|gb|AAO42448.1| putative short chain alcohol
           dehydrogenase [Arabidopsis thaliana]
           gi|332003577|gb|AED90960.1| tropine dehydrogenase
           [Arabidopsis thaliana]
          Length = 264

 Score =  385 bits (989), Expect = e-104
 Identities = 187/253 (73%), Positives = 218/253 (86%)
 Frame = +3

Query: 57  RWSLAGMTALVTGGTRGIGHAVVEELAELGAFVYTCSRNEQELNQRLEEWRVKGFNVDGS 236
           RWSLAG TALVTGGTRGIG AVVEELA+ GA V+TCSRN++ELN  L +W+  G  V GS
Sbjct: 6   RWSLAGKTALVTGGTRGIGRAVVEELAKFGAKVHTCSRNQEELNACLNDWKANGLVVSGS 65

Query: 237 VCDASNKTHRDQLFQRVSSTFNGKLNILVNNVGTNIRKGTAEYTAEEYSTIMATNLESSF 416
           VCDAS +  R++L Q  SS F+GKLNIL+NNVGTN+RK T EY++EEY+ IM+TNLES+F
Sbjct: 66  VCDASVRDQREKLIQEASSAFSGKLNILINNVGTNVRKPTVEYSSEEYAKIMSTNLESAF 125

Query: 417 HVCQLAYPLLKTSGSGCIVFISSVAGLVHIGSGSVYGATKGAINQLTKNLACEWAKDNIR 596
           H+ Q+A+PLLK SG G IVFISSVAGLVH+ SGS+YGATKGA+NQLT+NLACEWA DNIR
Sbjct: 126 HLSQIAHPLLKASGVGSIVFISSVAGLVHLSSGSIYGATKGALNQLTRNLACEWASDNIR 185

Query: 597 TNCVAPWYIKTSLVEHLLENKEFLDKVVARTPLRRPGEAEEVSSLVAYLCLPAASYITGQ 776
           TNCVAPWYIKTSLVE LLE KEF++ VV+RTPL R GE EEVSSLVA+LCLPA+SYITGQ
Sbjct: 186 TNCVAPWYIKTSLVETLLEKKEFVEAVVSRTPLGRVGEPEEVSSLVAFLCLPASSYITGQ 245

Query: 777 IIAVDGGFTVNSF 815
           +I+VDGGFTVN F
Sbjct: 246 VISVDGGFTVNGF 258


>ref|XP_002277835.1| PREDICTED: tropinone reductase homolog At1g07440 [Vitis vinifera]
           gi|296086189|emb|CBI31630.3| unnamed protein product
           [Vitis vinifera]
          Length = 268

 Score =  384 bits (987), Expect = e-104
 Identities = 184/254 (72%), Positives = 219/254 (86%)
 Frame = +3

Query: 54  SRWSLAGMTALVTGGTRGIGHAVVEELAELGAFVYTCSRNEQELNQRLEEWRVKGFNVDG 233
           S WSL G TALVTGGTRGIG+AVVEELA LG+ V+TCSRNE EL++ L EW  KGF V G
Sbjct: 11  SGWSLRGTTALVTGGTRGIGYAVVEELAGLGSTVHTCSRNEAELDKCLREWHAKGFAVTG 70

Query: 234 SVCDASNKTHRDQLFQRVSSTFNGKLNILVNNVGTNIRKGTAEYTAEEYSTIMATNLESS 413
           SVCD S++  R+QL ++VSS FNGKLNIL+NNVGTNIRK T ++TA EYSTIM TNLES+
Sbjct: 71  SVCDGSDRAQREQLMEKVSSIFNGKLNILINNVGTNIRKPTVDFTAAEYSTIMTTNLESA 130

Query: 414 FHVCQLAYPLLKTSGSGCIVFISSVAGLVHIGSGSVYGATKGAINQLTKNLACEWAKDNI 593
           +H+CQLA+PLLK SG+G IVFISSVAG++ +G+GS+Y ATK A+NQLTKNL+CEWAKDNI
Sbjct: 131 YHLCQLAHPLLKASGAGSIVFISSVAGVLSLGTGSIYAATKAAMNQLTKNLSCEWAKDNI 190

Query: 594 RTNCVAPWYIKTSLVEHLLENKEFLDKVVARTPLRRPGEAEEVSSLVAYLCLPAASYITG 773
           R+N VAPWYIKTSLVEHLL+ ++F + V++RTPLRRPGE +EVSSLVA+LCLP ASYITG
Sbjct: 191 RSNSVAPWYIKTSLVEHLLQKQDFFESVISRTPLRRPGEPKEVSSLVAFLCLPVASYITG 250

Query: 774 QIIAVDGGFTVNSF 815
           Q+I VDGG TVN F
Sbjct: 251 QVICVDGGMTVNGF 264