BLASTX nr result
ID: Bupleurum21_contig00013677
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00013677
(1076 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002285064.1| PREDICTED: probable carboxylesterase 2 [Viti... 186 1e-44
emb|CAN61111.1| hypothetical protein VITISV_006466 [Vitis vinifera] 184 3e-44
ref|XP_003550344.1| PREDICTED: probable carboxylesterase 13-like... 173 8e-41
ref|XP_002285075.1| PREDICTED: probable carboxylesterase 2-like ... 173 8e-41
ref|XP_002285076.1| PREDICTED: probable carboxylesterase 2-like ... 172 2e-40
>ref|XP_002285064.1| PREDICTED: probable carboxylesterase 2 [Vitis vinifera]
Length = 323
Score = 186 bits (471), Expect = 1e-44
Identities = 88/181 (48%), Positives = 121/181 (66%), Gaps = 2/181 (1%)
Frame = +2
Query: 536 IQWVALHSSSTGEEPWLNENVDFGRVFLGGDSAGGNITHNMAMRIGLEA--LGNMKIEGI 709
++W+ HS+S G EPWLN+ D R+F GDSAG N++HNMA+R G LG++K+ GI
Sbjct: 133 VKWLVSHSNSQGPEPWLNDYADLDRLFFAGDSAGANLSHNMAIRAGTRGHELGSVKVSGI 192
Query: 710 LLNHPEFWGSDANVDGKVIDPQNKEFVEKLWLFTYPEASGPDDPKLNPNMMTKLSCLGCK 889
+L HP FWG D V +V D Q K V+ LWLF P SG DDP +NP KL+ LGC+
Sbjct: 193 ILIHPYFWGKDP-VGAEVKDLQKKGLVDSLWLFVCPTTSGCDDPLINPATDPKLASLGCQ 251
Query: 890 RVLLLVAEHDFLNEWQWHYYHVLLESGWKGHTEILESRGEGHGFYLFDPTSDNSLAVVQK 1069
RVL+ VAE D L + W Y+ L +SGW G E++E+ GE H F+LF+PT D ++A++++
Sbjct: 252 RVLVFVAEKDTLRDRGWFYHETLGKSGWSGVVEVMEAEGEDHVFHLFNPTCDKAVAMLKQ 311
Query: 1070 M 1072
M
Sbjct: 312 M 312
Score = 56.2 bits (134), Expect(2) = 4e-14
Identities = 26/45 (57%), Positives = 32/45 (71%)
Frame = +3
Query: 264 VQSKDVIVTKETNVSARLYKPANVAPGKKLPLLIYFHGGGFVIES 398
V SKD+++ ET VSARLY P +KLPLL+YFHGG F IE+
Sbjct: 44 VNSKDIVIDPETGVSARLYIPKINDQSQKLPLLVYFHGGAFCIET 88
Score = 48.5 bits (114), Expect(2) = 4e-14
Identities = 20/42 (47%), Positives = 29/42 (69%)
Frame = +2
Query: 65 EILQEFRPLIRVYRDGRIERLAGTDLVPAGIDPNTGVQSKDV 190
E+ F P +RV++DG++ER GTD VP ++ TGV SKD+
Sbjct: 8 ELSFNFPPFLRVFKDGKVERFVGTDSVPPSLNIETGVNSKDI 49
>emb|CAN61111.1| hypothetical protein VITISV_006466 [Vitis vinifera]
Length = 323
Score = 184 bits (468), Expect = 3e-44
Identities = 88/181 (48%), Positives = 120/181 (66%), Gaps = 2/181 (1%)
Frame = +2
Query: 536 IQWVALHSSSTGEEPWLNENVDFGRVFLGGDSAGGNITHNMAMRIGLEA--LGNMKIEGI 709
++WV HS+S G EPWLN+ D +F GDSAG N++HNMA+R G LG++K+ GI
Sbjct: 133 VKWVVSHSNSQGPEPWLNDYADLDXLFFAGDSAGANLSHNMAIRAGTRGHELGSVKVSGI 192
Query: 710 LLNHPEFWGSDANVDGKVIDPQNKEFVEKLWLFTYPEASGPDDPKLNPNMMTKLSCLGCK 889
+L HP FWG D V +V D Q K V+ LWLF P SG DDP +NP KL+ LGC+
Sbjct: 193 ILIHPYFWGKDP-VGAEVKDLQKKGLVDSLWLFVCPTTSGCDDPLINPATDPKLASLGCQ 251
Query: 890 RVLLLVAEHDFLNEWQWHYYHVLLESGWKGHTEILESRGEGHGFYLFDPTSDNSLAVVQK 1069
RVL+ VAE D L + W Y+ L +SGW G E++E+ GE H F+LF+PT D ++A++++
Sbjct: 252 RVLVFVAEKDTLRDRGWFYHETLGKSGWSGVVEVMEAEGEDHVFHLFNPTCDKAVAMLKQ 311
Query: 1070 M 1072
M
Sbjct: 312 M 312
Score = 56.2 bits (134), Expect(2) = 4e-14
Identities = 26/45 (57%), Positives = 32/45 (71%)
Frame = +3
Query: 264 VQSKDVIVTKETNVSARLYKPANVAPGKKLPLLIYFHGGGFVIES 398
V SKD+++ ET VSARLY P +KLPLL+YFHGG F IE+
Sbjct: 44 VNSKDIVIEPETGVSARLYIPKINDQSQKLPLLVYFHGGAFCIET 88
Score = 48.5 bits (114), Expect(2) = 4e-14
Identities = 20/42 (47%), Positives = 29/42 (69%)
Frame = +2
Query: 65 EILQEFRPLIRVYRDGRIERLAGTDLVPAGIDPNTGVQSKDV 190
E+ F P +RV++DG++ER GTD VP ++ TGV SKD+
Sbjct: 8 ELSFNFPPFLRVFKDGKVERFVGTDSVPPSLNIETGVNSKDI 49
>ref|XP_003550344.1| PREDICTED: probable carboxylesterase 13-like [Glycine max]
Length = 337
Score = 173 bits (438), Expect = 8e-41
Identities = 84/182 (46%), Positives = 113/182 (62%), Gaps = 3/182 (1%)
Frame = +2
Query: 536 IQWVALHSSSTGEEPWLNENVDFGRVFLGGDSAGGNITHNMAMRIGLEALG-NMKIEGIL 712
+QWVA H + G+EPWLNE+ DFGRVFL GDSAG NI HN+ M +G M I G+
Sbjct: 144 LQWVASHRNKNGQEPWLNEHADFGRVFLAGDSAGANIVHNLTMLLGDPDWDIGMDILGVC 203
Query: 713 LNHPEFWGSDANVDGKVIDPQNKEFVEKLWLFTYPEASGPDDPKLNP--NMMTKLSCLGC 886
L HP FWGS + +DP+ K V++LW F PE + DDP++NP L LGC
Sbjct: 204 LVHPYFWGSVPVGSEEAVDPERKAVVDRLWRFVSPEMADKDDPRVNPVAEGAPSLGWLGC 263
Query: 887 KRVLLLVAEHDFLNEWQWHYYHVLLESGWKGHTEILESRGEGHGFYLFDPTSDNSLAVVQ 1066
+RVL+ VAE D L + W YY+ L SGW G E+ E+ GEGH F+L+D S + +++
Sbjct: 264 RRVLVCVAEKDVLRDRGWLYYNALSRSGWMGVVEVEETLGEGHAFHLYDLASHKAQCLIK 323
Query: 1067 KM 1072
++
Sbjct: 324 RL 325
Score = 41.6 bits (96), Expect(2) = 6e-06
Identities = 19/42 (45%), Positives = 28/42 (66%)
Frame = +2
Query: 65 EILQEFRPLIRVYRDGRIERLAGTDLVPAGIDPNTGVQSKDV 190
+I +F LIRV+ DGR++R GTD+VP P+ + SKD+
Sbjct: 16 QIAHDFPGLIRVFTDGRVQRFTGTDVVPPSTTPH--ITSKDI 55
Score = 35.4 bits (80), Expect(2) = 6e-06
Identities = 20/50 (40%), Positives = 27/50 (54%), Gaps = 5/50 (10%)
Frame = +3
Query: 264 VQSKDVIVTK--ETNVSARLYKPANVAPGKK---LPLLIYFHGGGFVIES 398
+ SKD+ + +SARL+ P ++ LPLLIYFHGG F S
Sbjct: 50 ITSKDITLLHPHSATLSARLFLPTPQTTSRRNNNLPLLIYFHGGAFCASS 99
>ref|XP_002285075.1| PREDICTED: probable carboxylesterase 2-like [Vitis vinifera]
Length = 323
Score = 173 bits (438), Expect = 8e-41
Identities = 83/186 (44%), Positives = 118/186 (63%), Gaps = 6/186 (3%)
Frame = +2
Query: 536 IQWVALHSSSTGEEPWLNENVDFGRVFLGGDSAGGNITHNMAMRIGLEALGNMKIEGILL 715
+QWV H + G EPWL ++ DF RVFL GDSAGGNI+HN+A++ G+E LG +K++GI +
Sbjct: 132 LQWVVSHCNGQGSEPWLKDHADFQRVFLAGDSAGGNISHNLAVQAGVEGLGGVKLQGICV 191
Query: 716 NHPEFWGSDANVDGKVID------PQNKEFVEKLWLFTYPEASGPDDPKLNPNMMTKLSC 877
HP F + GKV D P + V+ WL+ P SG +DP+ NP +L
Sbjct: 192 VHPYFGRKSEDDVGKVDDNASGGRPDVRPGVDNRWLYVCPTTSGFNDPRYNPAADERLWR 251
Query: 878 LGCKRVLLLVAEHDFLNEWQWHYYHVLLESGWKGHTEILESRGEGHGFYLFDPTSDNSLA 1057
LGC +VL+ VAE D L E W YY L +SGW G EI+E+ GEGH F+LF P+ + ++
Sbjct: 252 LGCSKVLVCVAEKDALRERGWFYYETLGKSGWSGEVEIMETEGEGHVFHLFKPSCERAVT 311
Query: 1058 VVQKMV 1075
+++++V
Sbjct: 312 LMKRIV 317
Score = 62.8 bits (151), Expect(2) = 1e-16
Identities = 27/41 (65%), Positives = 34/41 (82%)
Frame = +3
Query: 264 VQSKDVIVTKETNVSARLYKPANVAPGKKLPLLIYFHGGGF 386
V +KDV++ ET VSARL+KP +V P K+LPLL+YFHGGGF
Sbjct: 43 VSTKDVVIAPETGVSARLFKPNSVNPEKRLPLLVYFHGGGF 83
Score = 50.4 bits (119), Expect(2) = 1e-16
Identities = 22/42 (52%), Positives = 29/42 (69%)
Frame = +2
Query: 65 EILQEFRPLIRVYRDGRIERLAGTDLVPAGIDPNTGVQSKDV 190
E+ F P +RVY DG +ERL GTD+VP ++ TGV +KDV
Sbjct: 7 EVAYYFAPFLRVYTDGLVERLLGTDVVPPAMNSETGVSTKDV 48
>ref|XP_002285076.1| PREDICTED: probable carboxylesterase 2-like [Vitis vinifera]
Length = 323
Score = 172 bits (435), Expect = 2e-40
Identities = 83/186 (44%), Positives = 117/186 (62%), Gaps = 6/186 (3%)
Frame = +2
Query: 536 IQWVALHSSSTGEEPWLNENVDFGRVFLGGDSAGGNITHNMAMRIGLEALGNMKIEGILL 715
+QWV H + G EPWL ++ DF RVFL GDSAGGNI+HN+A++ G+E LG +K++GI +
Sbjct: 132 LQWVVSHCNGQGSEPWLKDHADFQRVFLAGDSAGGNISHNLAVQAGVEGLGGVKLQGICV 191
Query: 716 NHPEFWGSDANVDGKVID------PQNKEFVEKLWLFTYPEASGPDDPKLNPNMMTKLSC 877
HP F + GKV D P + V+ WL+ P SG +DP+ NP +L
Sbjct: 192 VHPYFGRKSEDDVGKVDDNASGGRPDVRPGVDNWWLYACPTTSGFNDPRYNPAADERLWR 251
Query: 878 LGCKRVLLLVAEHDFLNEWQWHYYHVLLESGWKGHTEILESRGEGHGFYLFDPTSDNSLA 1057
LGC +VL+ VAE D L E W YY L +SGW G EI+E+ GEGH F+LF P+ ++
Sbjct: 252 LGCSKVLVCVAEKDALRERGWFYYETLGKSGWSGEVEIMETEGEGHVFHLFKPSCGRAVT 311
Query: 1058 VVQKMV 1075
+++++V
Sbjct: 312 LMKRIV 317
Score = 62.8 bits (151), Expect(2) = 8e-17
Identities = 27/41 (65%), Positives = 34/41 (82%)
Frame = +3
Query: 264 VQSKDVIVTKETNVSARLYKPANVAPGKKLPLLIYFHGGGF 386
V +KDV++ ET VSARL+KP +V P K+LPLL+YFHGGGF
Sbjct: 43 VSTKDVVIAPETGVSARLFKPNSVNPEKRLPLLVYFHGGGF 83
Score = 51.2 bits (121), Expect(2) = 8e-17
Identities = 22/42 (52%), Positives = 29/42 (69%)
Frame = +2
Query: 65 EILQEFRPLIRVYRDGRIERLAGTDLVPAGIDPNTGVQSKDV 190
E+ F P +RVY DG +ERL GTD+VP ++ TGV +KDV
Sbjct: 7 EVAYNFAPFLRVYTDGLVERLIGTDVVPPAMNSETGVSTKDV 48