BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00013522
         (1074 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAA81892.1| epoxide hydrolase [Solanum tuberosum]                  453   e-125
pdb|2CJP|A Chain A, Structure Of Potato (Solanum Tuberosum) Epox...   453   e-125
pdb|3CXU|A Chain A, Structure Of A Y149f Mutant Of Epoxide Hydro...   452   e-125
gb|AAA81889.1| epoxide hydrolase [Solanum tuberosum]                  451   e-124
gb|AAA81891.1| epoxide hydrolase [Solanum tuberosum]                  447   e-123

>gb|AAA81892.1| epoxide hydrolase [Solanum tuberosum]
          Length = 321

 Score =  453 bits (1166), Expect = e-125
 Identities = 210/323 (65%), Positives = 263/323 (81%), Gaps = 3/323 (0%)
 Frame = +2

Query: 26  MEGIEHKMISVNGLNMHIAEKGKGPLVLFIHGFPQLWYQWRRQIEFMAARGYHAVAPDLR 205
           ME IEHKM++VNGLNMH+AE G+GP +LFIHGFP+LWY WR Q+ ++A RGY AVAPDLR
Sbjct: 1   MEKIEHKMVAVNGLNMHLAELGEGPTILFIHGFPELWYSWRHQMVYLAERGYRAVAPDLR 60

Query: 206 GYGDTTGAPLDDPTKFTMMHLVGDMVALLDVVAP-DEKVFVVAHDWGAYISWHLSLFRPD 382
           GYGDTTGAPL+DP+KF+++HLVGD+VALL+ +AP +EKVFVVAHDWGA I+WHL LFRPD
Sbjct: 61  GYGDTTGAPLNDPSKFSILHLVGDVVALLEAIAPNEEKVFVVAHDWGALIAWHLCLFRPD 120

Query: 383 KVKAIVSLGVQYMPRHPVARTVDFMKLFYGEDHYMVRFQEPGDMEAEIADNIGTKTFIKQ 562
           KVKA+V+L V +  R+P    V+ +K  YGEDHY+ RFQ PG++EAE A  IG K+ +K+
Sbjct: 121 KVKALVNLSVHFSKRNPKMNVVEGLKAIYGEDHYISRFQVPGEIEAEFAP-IGAKSVLKK 179

Query: 563 ILSHRDPSPWFFPKGKGFNGDTTDSP--LPSWLSEDDLDYYTSKFEKTGFTGAFNYYRAF 736
           IL++RDP+P++FPKGKG      D+P  L SWLSE++LDYY +KFE+TGFTGA NYYRA 
Sbjct: 180 ILTYRDPAPFYFPKGKGLEA-IPDAPVALSSWLSEEELDYYANKFEQTGFTGAVNYYRAL 238

Query: 737 DLTWELTAPWTGAQVKVPAKHIVGDLDMTYAIPGSKEFIHNGELNKYVPLLQEVVVMEGV 916
            + WELTAPWTGAQVKVP K IVG+ D+ Y IPG+KE+IHNG   K VPLL+EVVV+EG 
Sbjct: 239 PINWELTAPWTGAQVKVPTKFIVGEFDLVYHIPGAKEYIHNGGFKKDVPLLEEVVVLEGA 298

Query: 917 AHFITEEKPDEINNHIYQFFHNF 985
           AHF+++E+P EI+ HIY F   F
Sbjct: 299 AHFVSQERPHEISKHIYDFIQKF 321


>pdb|2CJP|A Chain A, Structure Of Potato (Solanum Tuberosum) Epoxide Hydrolase
           I (Steh1) gi|110590994|pdb|2CJP|B Chain B, Structure Of
           Potato (Solanum Tuberosum) Epoxide Hydrolase I (Steh1)
          Length = 328

 Score =  453 bits (1166), Expect = e-125
 Identities = 210/326 (64%), Positives = 264/326 (80%), Gaps = 3/326 (0%)
 Frame = +2

Query: 17  HIKMEGIEHKMISVNGLNMHIAEKGKGPLVLFIHGFPQLWYQWRRQIEFMAARGYHAVAP 196
           H  M+ IEHKM++VNGLNMH+AE G+GP +LFIHGFP+LWY WR Q+ ++A RGY AVAP
Sbjct: 5   HHHMKKIEHKMVAVNGLNMHLAELGEGPTILFIHGFPELWYSWRHQMVYLAERGYRAVAP 64

Query: 197 DLRGYGDTTGAPLDDPTKFTMMHLVGDMVALLDVVAP-DEKVFVVAHDWGAYISWHLSLF 373
           DLRGYGDTTGAPL+DP+KF+++HLVGD+VALL+ +AP +EKVFVVAHDWGA I+WHL LF
Sbjct: 65  DLRGYGDTTGAPLNDPSKFSILHLVGDVVALLEAIAPNEEKVFVVAHDWGALIAWHLCLF 124

Query: 374 RPDKVKAIVSLGVQYMPRHPVARTVDFMKLFYGEDHYMVRFQEPGDMEAEIADNIGTKTF 553
           RPDKVKA+V+L V +  R+P    V+ +K  YGEDHY+ RFQ PG++EAE A  IG K+ 
Sbjct: 125 RPDKVKALVNLSVHFSKRNPKMNVVEGLKAIYGEDHYISRFQVPGEIEAEFAP-IGAKSV 183

Query: 554 IKQILSHRDPSPWFFPKGKGFNGDTTDSP--LPSWLSEDDLDYYTSKFEKTGFTGAFNYY 727
           +K+IL++RDP+P++FPKGKG      D+P  L SWLSE++LDYY +KFE+TGFTGA NYY
Sbjct: 184 LKKILTYRDPAPFYFPKGKGLEA-IPDAPVALSSWLSEEELDYYANKFEQTGFTGAVNYY 242

Query: 728 RAFDLTWELTAPWTGAQVKVPAKHIVGDLDMTYAIPGSKEFIHNGELNKYVPLLQEVVVM 907
           RA  + WELTAPWTGAQVKVP K IVG+ D+ Y IPG+KE+IHNG   K VPLL+EVVV+
Sbjct: 243 RALPINWELTAPWTGAQVKVPTKFIVGEFDLVYHIPGAKEYIHNGGFKKDVPLLEEVVVL 302

Query: 908 EGVAHFITEEKPDEINNHIYQFFHNF 985
           EG AHF+++E+P EI+ HIY F   F
Sbjct: 303 EGAAHFVSQERPHEISKHIYDFIQKF 328


>pdb|3CXU|A Chain A, Structure Of A Y149f Mutant Of Epoxide Hydrolase From
           Solanum Tuberosum gi|193885363|pdb|3CXU|B Chain B,
           Structure Of A Y149f Mutant Of Epoxide Hydrolase From
           Solanum Tuberosum
          Length = 328

 Score =  452 bits (1162), Expect = e-125
 Identities = 209/326 (64%), Positives = 264/326 (80%), Gaps = 3/326 (0%)
 Frame = +2

Query: 17  HIKMEGIEHKMISVNGLNMHIAEKGKGPLVLFIHGFPQLWYQWRRQIEFMAARGYHAVAP 196
           H  M+ IEHKM++VNGLNMH+AE G+GP +LFIHGFP+LWY WR Q+ ++A RGY AVAP
Sbjct: 5   HHHMKKIEHKMVAVNGLNMHLAELGEGPTILFIHGFPELWYSWRHQMVYLAERGYRAVAP 64

Query: 197 DLRGYGDTTGAPLDDPTKFTMMHLVGDMVALLDVVAP-DEKVFVVAHDWGAYISWHLSLF 373
           DLRGYGDTTGAPL+DP+KF+++HLVGD+VALL+ +AP +EKVFVVAHDWGA I+WHL LF
Sbjct: 65  DLRGYGDTTGAPLNDPSKFSILHLVGDVVALLEAIAPNEEKVFVVAHDWGALIAWHLCLF 124

Query: 374 RPDKVKAIVSLGVQYMPRHPVARTVDFMKLFYGEDHYMVRFQEPGDMEAEIADNIGTKTF 553
           RPDKVKA+V+L V +  R+P    V+ +K  +GEDHY+ RFQ PG++EAE A  IG K+ 
Sbjct: 125 RPDKVKALVNLSVHFSKRNPKMNVVEGLKAIFGEDHYISRFQVPGEIEAEFAP-IGAKSV 183

Query: 554 IKQILSHRDPSPWFFPKGKGFNGDTTDSP--LPSWLSEDDLDYYTSKFEKTGFTGAFNYY 727
           +K+IL++RDP+P++FPKGKG      D+P  L SWLSE++LDYY +KFE+TGFTGA NYY
Sbjct: 184 LKKILTYRDPAPFYFPKGKGLEA-IPDAPVALSSWLSEEELDYYANKFEQTGFTGAVNYY 242

Query: 728 RAFDLTWELTAPWTGAQVKVPAKHIVGDLDMTYAIPGSKEFIHNGELNKYVPLLQEVVVM 907
           RA  + WELTAPWTGAQVKVP K IVG+ D+ Y IPG+KE+IHNG   K VPLL+EVVV+
Sbjct: 243 RALPINWELTAPWTGAQVKVPTKFIVGEFDLVYHIPGAKEYIHNGGFKKDVPLLEEVVVL 302

Query: 908 EGVAHFITEEKPDEINNHIYQFFHNF 985
           EG AHF+++E+P EI+ HIY F   F
Sbjct: 303 EGAAHFVSQERPHEISKHIYDFIQKF 328


>gb|AAA81889.1| epoxide hydrolase [Solanum tuberosum]
          Length = 321

 Score =  451 bits (1161), Expect = e-124
 Identities = 208/323 (64%), Positives = 264/323 (81%), Gaps = 3/323 (0%)
 Frame = +2

Query: 26  MEGIEHKMISVNGLNMHIAEKGKGPLVLFIHGFPQLWYQWRRQIEFMAARGYHAVAPDLR 205
           ME IEHKM++VNGLNMHIAE G+GP +LF+HGFP+LWY WR Q+ ++A  GY AVAPDLR
Sbjct: 1   MEKIEHKMVAVNGLNMHIAELGQGPTILFLHGFPELWYSWRHQMVYLAECGYRAVAPDLR 60

Query: 206 GYGDTTGAPLDDPTKFTMMHLVGDMVALLDVVAP-DEKVFVVAHDWGAYISWHLSLFRPD 382
           GYGDTTGA L+DP+KF+++HLVGD+VALL+ +AP +EKVFVVAHDWGA I+WHL LFRPD
Sbjct: 61  GYGDTTGASLNDPSKFSILHLVGDVVALLEAIAPNEEKVFVVAHDWGALIAWHLCLFRPD 120

Query: 383 KVKAIVSLGVQYMPRHPVARTVDFMKLFYGEDHYMVRFQEPGDMEAEIADNIGTKTFIKQ 562
           KVKA+V+L V ++PR+P   TV+++K  YGEDHY+ RFQ PG++EAE A  IG K+ +K+
Sbjct: 121 KVKALVNLSVHFLPRNPKMNTVEWLKAIYGEDHYISRFQVPGEIEAEFAP-IGAKSILKK 179

Query: 563 ILSHRDPSPWFFPKGKGFNGDTTDSP--LPSWLSEDDLDYYTSKFEKTGFTGAFNYYRAF 736
           IL++RDP+P++FPKGKG      D+P  L SWLSE++LDYY +KFE+TGFTG  NYYRA 
Sbjct: 180 ILTYRDPAPFYFPKGKGLEA-LPDAPVALSSWLSEEELDYYANKFEQTGFTGGVNYYRAL 238

Query: 737 DLTWELTAPWTGAQVKVPAKHIVGDLDMTYAIPGSKEFIHNGELNKYVPLLQEVVVMEGV 916
            ++WELTAPWTGAQVKVP K IVG+ D+ Y IPG+KE+IHNG   + VPLL+EVVV+EG 
Sbjct: 239 PISWELTAPWTGAQVKVPTKFIVGEFDLVYHIPGAKEYIHNGGFKEDVPLLEEVVVLEGS 298

Query: 917 AHFITEEKPDEINNHIYQFFHNF 985
           AHF+ +E+P EI+ HIY F   F
Sbjct: 299 AHFVNQERPHEISKHIYDFIQKF 321


>gb|AAA81891.1| epoxide hydrolase [Solanum tuberosum]
          Length = 321

 Score =  447 bits (1151), Expect = e-123
 Identities = 208/323 (64%), Positives = 260/323 (80%), Gaps = 3/323 (0%)
 Frame = +2

Query: 26  MEGIEHKMISVNGLNMHIAEKGKGPLVLFIHGFPQLWYQWRRQIEFMAARGYHAVAPDLR 205
           ME IEHKM++VNGLNMHIAE G+GP +LF+HGFP+LWY WR Q+ ++A RGY AVAP LR
Sbjct: 1   MEKIEHKMVAVNGLNMHIAELGQGPTILFLHGFPELWYSWRHQMVYLAERGYRAVAPVLR 60

Query: 206 GYGDTTGAPLDDPTKFTMMHLVGDMVALLDVVAP-DEKVFVVAHDWGAYISWHLSLFRPD 382
           GYGDTTGAPL+DP+KF+++ LVGD+VALL+ +AP +EKVFVVAHDWGA I+WHL LFRPD
Sbjct: 61  GYGDTTGAPLNDPSKFSILQLVGDVVALLEAIAPNEEKVFVVAHDWGALIAWHLCLFRPD 120

Query: 383 KVKAIVSLGVQYMPRHPVARTVDFMKLFYGEDHYMVRFQEPGDMEAEIADNIGTKTFIKQ 562
           KVKA+V+  V ++PR+P   TV+ +K  YGEDHY+ RFQ PG++EAE A  IG K+ +K+
Sbjct: 121 KVKALVNSSVHFLPRNPKMNTVEGLKAVYGEDHYISRFQVPGEIEAEFAP-IGAKSVLKK 179

Query: 563 ILSHRDPSPWFFPKGKGFNGDTTDSP--LPSWLSEDDLDYYTSKFEKTGFTGAFNYYRAF 736
           IL+ RDP+P++FPKGKG      D+P  L SWLSE++LDYY +KFE+TGFTG  NYYRA 
Sbjct: 180 ILTFRDPAPFYFPKGKGLEA-LPDAPVALSSWLSEEELDYYANKFEQTGFTGGVNYYRAL 238

Query: 737 DLTWELTAPWTGAQVKVPAKHIVGDLDMTYAIPGSKEFIHNGELNKYVPLLQEVVVMEGV 916
            + WELTAPWTGAQVKVP K IVG+ D+ Y IPG+KE+IHNG   K VPLL+EVVV+EG 
Sbjct: 239 SINWELTAPWTGAQVKVPTKFIVGEFDLVYHIPGAKEYIHNGGFKKDVPLLEEVVVLEGA 298

Query: 917 AHFITEEKPDEINNHIYQFFHNF 985
           AHF+ +E+P EI+ HIY F   F
Sbjct: 299 AHFVNQERPHEISKHIYDFIQKF 321