BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00013427
         (1757 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AED99870.1| cytochrome P450 [Panax notoginseng]                    724   0.0  
gb|ADO51747.1| fatty acid hydroperoxide lyase [Camellia sinensis]     662   0.0  
gb|AAO72740.1| fatty acid hydroperoxide lyase [Citrus sinensis]       637   e-180
dbj|BAC55161.1| hydroperoxide lyase [Citrus jambhiri]                 637   e-180
gb|ADF57569.1| hydroperoxide lyase [Citrus japonica var. margarita]   637   e-180

>gb|AED99870.1| cytochrome P450 [Panax notoginseng]
          Length = 485

 Score =  724 bits (1869), Expect(2) = 0.0
 Identities = 341/432 (78%), Positives = 388/432 (89%)
 Frame = -1

Query: 1571 IRTIPGGYGLPVLGPFVDRLSYFWFQGAETYFKKRIEEHKSTVFRTNIPPTFPLFMRVNP 1392
            +RTIPGGYG P LGP  DRLSYFWFQG ET+F KRIE+HKSTVFRTNIPPT PLF  VNP
Sbjct: 28   VRTIPGGYGWPFLGPISDRLSYFWFQGPETFFSKRIEQHKSTVFRTNIPPTSPLFFGVNP 87

Query: 1391 NVIAVLDCKSFAHLFDMEVVEKKNILVGDFMPSTSFTGDIRVCAYLDTSEPQHSQVKKFA 1212
            NVIAVLDCKSF+HLFDME++EKKNIL+GDFMPSTSFTGD+RVCAYLDTSEP+H+Q+KKFA
Sbjct: 88   NVIAVLDCKSFSHLFDMELMEKKNILIGDFMPSTSFTGDLRVCAYLDTSEPRHAQIKKFA 147

Query: 1211 MEILKRSSKIWIPTVTKNLDTMWDTIESDVSSSGSAAPLIPLQKFTFNFLTRCLIGVDPS 1032
            M+ LKRSS IW+PTVT NLDTMWD+IESD++ SGS+ P +PLQKF F+FLTRCL+GVDPS
Sbjct: 148  MDXLKRSSSIWVPTVTTNLDTMWDSIESDIAKSGSSTPFVPLQKFIFSFLTRCLVGVDPS 207

Query: 1031 VSTEVAEWGYFWLDKWLALQIIPTAKIGILQPFEEIFLHSFAYPSFLVTGDYYKLVEFIE 852
            +S EVA+WGY WLDKWLALQI+PT  +G LQP EEIFLHSFAYPSFLVTGDY KL +F+E
Sbjct: 208  ISPEVADWGYTWLDKWLALQILPTLNVGFLQPLEEIFLHSFAYPSFLVTGDYNKLAKFVE 267

Query: 851  KEGGEVLELAQSEFGLSKKEAVHNLMFILGFNAFGGFSVFLPSIFTVLGTDKTGLQSKLR 672
            KE  EVL+  Q+EFGL+K+EA+HNL+FILGFNAFGGFS+F PS+F+ LG+D T LQ KLR
Sbjct: 268  KEAQEVLKRGQTEFGLTKQEALHNLLFILGFNAFGGFSIFFPSVFSKLGSD-TALQEKLR 326

Query: 671  KEVKEKIGSNSLSFDSVSQLELVNSFVYETLRLNPPVPLQFGRARKDFTLSSHDSAFEIK 492
            KEV+E  G++ L+F+SV +LELVNSFVYETLRLNPPVPLQFGRARKDF LSSHDS FEIK
Sbjct: 327  KEVREITGTSPLTFESVKELELVNSFVYETLRLNPPVPLQFGRARKDFKLSSHDSVFEIK 386

Query: 491  KGELLCGYQPLVMRDEKVFDEPEKFVVDRFTKEKGSELLSYLFWSNGPQNGSPNESNKQC 312
            KGELLCGYQPLVM+D KVFD+PEKFV DRFTKEKG ELLSYL+WSNGPQ GSP+ESNKQC
Sbjct: 387  KGELLCGYQPLVMKDGKVFDDPEKFVPDRFTKEKGRELLSYLYWSNGPQTGSPSESNKQC 446

Query: 311  AAKDYVTLTASV 276
            AAKDYVTLTA +
Sbjct: 447  AAKDYVTLTAFI 458



 Score = 39.7 bits (91), Expect(2) = 0.0
 Identities = 19/30 (63%), Positives = 23/30 (76%)
 Frame = -3

Query: 189 TASVLVAHMLLRYESVTFDSSGSILAVEKA 100
           TA + VAH+  RY+S T +SSGSI AVEKA
Sbjct: 455 TAFIFVAHLFKRYDSFTVNSSGSITAVEKA 484


>gb|ADO51747.1| fatty acid hydroperoxide lyase [Camellia sinensis]
          Length = 491

 Score =  662 bits (1709), Expect(2) = 0.0
 Identities = 313/430 (72%), Positives = 371/430 (86%)
 Frame = -1

Query: 1571 IRTIPGGYGLPVLGPFVDRLSYFWFQGAETYFKKRIEEHKSTVFRTNIPPTFPLFMRVNP 1392
            +RTIPGGYG PVLGP  DRL YFWFQG ET+F+KRIE+HKSTVFRTN+PPTFP F  VNP
Sbjct: 34   VRTIPGGYGWPVLGPISDRLDYFWFQGPETFFRKRIEKHKSTVFRTNLPPTFPFFYGVNP 93

Query: 1391 NVIAVLDCKSFAHLFDMEVVEKKNILVGDFMPSTSFTGDIRVCAYLDTSEPQHSQVKKFA 1212
            NV+A+LDCKSFAH+F+ME+VEKKN+LVGDFMPS S+TGD+RVCAYLDTSE  HS+VK FA
Sbjct: 94   NVVALLDCKSFAHMFNMEIVEKKNVLVGDFMPSVSYTGDLRVCAYLDTSESLHSKVKNFA 153

Query: 1211 MEILKRSSKIWIPTVTKNLDTMWDTIESDVSSSGSAAPLIPLQKFTFNFLTRCLIGVDPS 1032
            ++ILKRSS IW+PT++  LDTMW +IES ++ SGSA+ L+P+Q+F F+F TR LIG D +
Sbjct: 154  LDILKRSSTIWVPTLSSTLDTMWSSIESSLAMSGSASYLVPIQQFIFSFFTRTLIGADTA 213

Query: 1031 VSTEVAEWGYFWLDKWLALQIIPTAKIGILQPFEEIFLHSFAYPSFLVTGDYYKLVEFIE 852
             S E+A  GY  LD WLALQ++PT KIGILQP EE+FLHS+AYP FLV+G Y KLV+FIE
Sbjct: 214  ASPEIASSGYAMLDIWLALQLLPTVKIGILQPLEELFLHSYAYPFFLVSGGYNKLVKFIE 273

Query: 851  KEGGEVLELAQSEFGLSKKEAVHNLMFILGFNAFGGFSVFLPSIFTVLGTDKTGLQSKLR 672
            + G EV++  ++EFGL+K E +HNL+FILGFNA+GGFS+FLP++ + LGTD TG+Q KLR
Sbjct: 274  EHGKEVIQRGETEFGLTKHETIHNLLFILGFNAYGGFSIFLPTLLSQLGTDTTGIQQKLR 333

Query: 671  KEVKEKIGSNSLSFDSVSQLELVNSFVYETLRLNPPVPLQFGRARKDFTLSSHDSAFEIK 492
            +EV+ K GS +LSFDSV ++ELV SFVYETLRLNPPVPLQ+ RARKDF LSSHDSA+EIK
Sbjct: 334  EEVRAKSGS-TLSFDSVKEMELVKSFVYETLRLNPPVPLQYARARKDFILSSHDSAYEIK 392

Query: 491  KGELLCGYQPLVMRDEKVFDEPEKFVVDRFTKEKGSELLSYLFWSNGPQNGSPNESNKQC 312
            KGELLCGYQ LVMRD KVFD PEKF+ DRFTKEKGSELLSYL+WSNGPQ GSP ESNKQC
Sbjct: 393  KGELLCGYQTLVMRDSKVFDNPEKFIFDRFTKEKGSELLSYLYWSNGPQTGSPCESNKQC 452

Query: 311  AAKDYVTLTA 282
            AAKDYVTLTA
Sbjct: 453  AAKDYVTLTA 462



 Score = 38.9 bits (89), Expect(2) = 0.0
 Identities = 18/30 (60%), Positives = 24/30 (80%)
 Frame = -3

Query: 189 TASVLVAHMLLRYESVTFDSSGSILAVEKA 100
           TA + VAH+  RY+S+T +SSG+I AVEKA
Sbjct: 461 TACLFVAHLYRRYDSITCNSSGAITAVEKA 490


>gb|AAO72740.1| fatty acid hydroperoxide lyase [Citrus sinensis]
          Length = 499

 Score =  637 bits (1643), Expect = e-180
 Identities = 301/443 (67%), Positives = 361/443 (81%)
 Frame = -1

Query: 1577 LAIRTIPGGYGLPVLGPFVDRLSYFWFQGAETYFKKRIEEHKSTVFRTNIPPTFPLFMRV 1398
            L +RTIPG YG P+LGP  DRL YFWFQG ET+F+KRIE+HKSTVFRTNIPPT+PLF+ V
Sbjct: 40   LPLRTIPGSYGWPLLGPISDRLDYFWFQGPETFFRKRIEKHKSTVFRTNIPPTWPLFLNV 99

Query: 1397 NPNVIAVLDCKSFAHLFDMEVVEKKNILVGDFMPSTSFTGDIRVCAYLDTSEPQHSQVKK 1218
            NPNVIAVLDCKSFAHLFDME+VEKKNILVGDFMPS  FTG +RV AYLDTSEP+H+Q+K 
Sbjct: 100  NPNVIAVLDCKSFAHLFDMEIVEKKNILVGDFMPSVKFTGGLRVSAYLDTSEPKHAQLKG 159

Query: 1217 FAMEILKRSSKIWIPTVTKNLDTMWDTIESDVSSSGSAAPLIPLQKFTFNFLTRCLIGVD 1038
            FAM+ LKRSSK+W+  V  +LDT++DT+E ++S   S + ++PLQK  FNFL++ ++G D
Sbjct: 160  FAMDTLKRSSKLWVSEVVSSLDTLFDTVEKELSEKNSISYMVPLQKCVFNFLSKSIVGAD 219

Query: 1037 PSVSTEVAEWGYFWLDKWLALQIIPTAKIGILQPFEEIFLHSFAYPSFLVTGDYYKLVEF 858
            P    E+AE G+  LDKWLALQI+PT  I ILQP EEIFLHSFAYP  LV+GDY KL  F
Sbjct: 220  PKADAEIAENGFSMLDKWLALQILPTVSINILQPLEEIFLHSFAYPFALVSGDYNKLHNF 279

Query: 857  IEKEGGEVLELAQSEFGLSKKEAVHNLMFILGFNAFGGFSVFLPSIFTVLGTDKTGLQSK 678
            +EKEG EV++  Q EFGL+K+EA+HNL+FILGFNAFGGFS+ LP +   + +D TGLQ+K
Sbjct: 280  VEKEGKEVVQRGQDEFGLTKEEAIHNLLFILGFNAFGGFSILLPKLINAIASDTTGLQAK 339

Query: 677  LRKEVKEKIGSNSLSFDSVSQLELVNSFVYETLRLNPPVPLQFGRARKDFTLSSHDSAFE 498
            LR EVKEK G+++L+F+SV  LELV S VYETLRLNPPVPLQF RARKDF LSS+DS ++
Sbjct: 340  LRSEVKEKCGTSALTFESVKSLELVQSVVYETLRLNPPVPLQFARARKDFQLSSYDSVYD 399

Query: 497  IKKGELLCGYQPLVMRDEKVFDEPEKFVVDRFTKEKGSELLSYLFWSNGPQNGSPNESNK 318
            IKKGELLCGYQPLVMRD KVFD+ E F  +RF  EKGSELLSYL+WSNGPQ G+PN+ NK
Sbjct: 400  IKKGELLCGYQPLVMRDSKVFDDAESFKAERFMGEKGSELLSYLYWSNGPQTGTPNDMNK 459

Query: 317  QCAAKDYVTLTASVLFFFFDGKY 249
            QCA KDYVTL A +   +   +Y
Sbjct: 460  QCAGKDYVTLVACLTVAYVFQRY 482


>dbj|BAC55161.1| hydroperoxide lyase [Citrus jambhiri]
          Length = 499

 Score =  637 bits (1643), Expect = e-180
 Identities = 299/443 (67%), Positives = 363/443 (81%)
 Frame = -1

Query: 1577 LAIRTIPGGYGLPVLGPFVDRLSYFWFQGAETYFKKRIEEHKSTVFRTNIPPTFPLFMRV 1398
            L +RTIPG YG P+LGP  DRL YFWFQG ET+F+KRIE+HKSTVFRTNIPPT+PLF+ V
Sbjct: 40   LPLRTIPGSYGWPLLGPISDRLDYFWFQGPETFFRKRIEKHKSTVFRTNIPPTWPLFLNV 99

Query: 1397 NPNVIAVLDCKSFAHLFDMEVVEKKNILVGDFMPSTSFTGDIRVCAYLDTSEPQHSQVKK 1218
            NPNVIAVLDCKSFAHLFDME+VEKKNILVGDFMPS  FTG +RV AYLDTSEP+H+Q+K 
Sbjct: 100  NPNVIAVLDCKSFAHLFDMEIVEKKNILVGDFMPSVKFTGGLRVSAYLDTSEPKHAQLKG 159

Query: 1217 FAMEILKRSSKIWIPTVTKNLDTMWDTIESDVSSSGSAAPLIPLQKFTFNFLTRCLIGVD 1038
            FAM+ LKRSSK+W+  V  +LDT++DT+E ++S   S + ++PLQK  FNFL++ ++G D
Sbjct: 160  FAMDTLKRSSKLWVSEVVSSLDTLFDTVEKELSEKNSISYMVPLQKCVFNFLSKSIVGAD 219

Query: 1037 PSVSTEVAEWGYFWLDKWLALQIIPTAKIGILQPFEEIFLHSFAYPSFLVTGDYYKLVEF 858
            P    E+AE G+  LDKWLALQI+PT  I ILQP EEIFLHSFAYP  LV+GDY KL  F
Sbjct: 220  PKADAEIAENGFSMLDKWLALQIVPTVSINILQPLEEIFLHSFAYPFALVSGDYSKLHNF 279

Query: 857  IEKEGGEVLELAQSEFGLSKKEAVHNLMFILGFNAFGGFSVFLPSIFTVLGTDKTGLQSK 678
            +EKEG EV++  Q EFGL+K+EA+HNL+FILGFNAFGGFS+F+P +   + +D TGLQ+K
Sbjct: 280  VEKEGKEVVQRGQDEFGLTKEEAIHNLLFILGFNAFGGFSIFVPKLINAIASDTTGLQAK 339

Query: 677  LRKEVKEKIGSNSLSFDSVSQLELVNSFVYETLRLNPPVPLQFGRARKDFTLSSHDSAFE 498
            LR EVKE+ G+++L+F+SV  LELV S VYETLRLNPPVPLQF RARKDF LSS+DS ++
Sbjct: 340  LRSEVKERCGTSALTFESVKSLELVQSVVYETLRLNPPVPLQFARARKDFQLSSYDSVYD 399

Query: 497  IKKGELLCGYQPLVMRDEKVFDEPEKFVVDRFTKEKGSELLSYLFWSNGPQNGSPNESNK 318
            IKKGELLCGYQPLVMRD KVFD+ E F  +RF  +KGSELLSYL+WSNGPQ G+PN+ NK
Sbjct: 400  IKKGELLCGYQPLVMRDSKVFDDAESFKAERFMGKKGSELLSYLYWSNGPQTGTPNDMNK 459

Query: 317  QCAAKDYVTLTASVLFFFFDGKY 249
            QCA KDYVTL A ++  +   +Y
Sbjct: 460  QCAGKDYVTLVACLIVAYVFQRY 482


>gb|ADF57569.1| hydroperoxide lyase [Citrus japonica var. margarita]
          Length = 499

 Score =  637 bits (1643), Expect = e-180
 Identities = 301/443 (67%), Positives = 361/443 (81%)
 Frame = -1

Query: 1577 LAIRTIPGGYGLPVLGPFVDRLSYFWFQGAETYFKKRIEEHKSTVFRTNIPPTFPLFMRV 1398
            L +RTIPG YG P+LGP  DRL YFWFQG ET+F+KRIE+HKSTVFRTNIPPT+PLF+ V
Sbjct: 40   LPLRTIPGSYGWPLLGPISDRLDYFWFQGPETFFRKRIEKHKSTVFRTNIPPTWPLFLNV 99

Query: 1397 NPNVIAVLDCKSFAHLFDMEVVEKKNILVGDFMPSTSFTGDIRVCAYLDTSEPQHSQVKK 1218
            NPNVIAVLDCKSFAHLFDME+VEKKNILVGDFMPS  FTG +RV AYLDTSEP+H+Q+K 
Sbjct: 100  NPNVIAVLDCKSFAHLFDMEIVEKKNILVGDFMPSVKFTGGLRVSAYLDTSEPKHAQLKG 159

Query: 1217 FAMEILKRSSKIWIPTVTKNLDTMWDTIESDVSSSGSAAPLIPLQKFTFNFLTRCLIGVD 1038
            FAM+ LKRSSK+W+  V  +LDT++DT+E ++S   S + ++PLQK  FNFL++ ++G D
Sbjct: 160  FAMDTLKRSSKLWVSEVVSSLDTLFDTVEKELSEKNSISYVVPLQKCVFNFLSKSIVGAD 219

Query: 1037 PSVSTEVAEWGYFWLDKWLALQIIPTAKIGILQPFEEIFLHSFAYPSFLVTGDYYKLVEF 858
            P    E+AE G+  LDKWLALQI+PT  I ILQP EEIFLHSFAYP  LV+GDY KL  F
Sbjct: 220  PKADAEIAENGFSMLDKWLALQILPTVSINILQPLEEIFLHSFAYPFALVSGDYNKLYNF 279

Query: 857  IEKEGGEVLELAQSEFGLSKKEAVHNLMFILGFNAFGGFSVFLPSIFTVLGTDKTGLQSK 678
            +EKEG EV++  Q EFGL+K+E +HNL+FILGFNAFGGFS+FLP +   + +D TGLQ+K
Sbjct: 280  VEKEGKEVVQRGQDEFGLTKEEVIHNLLFILGFNAFGGFSIFLPKLINAIASDTTGLQAK 339

Query: 677  LRKEVKEKIGSNSLSFDSVSQLELVNSFVYETLRLNPPVPLQFGRARKDFTLSSHDSAFE 498
            LR EVKEK G+++L+F+SV  LELV S V ETLRLNPPVPLQF RARKDF LSS+DS ++
Sbjct: 340  LRSEVKEKCGTSALTFESVKSLELVQSVVCETLRLNPPVPLQFARARKDFQLSSYDSVYD 399

Query: 497  IKKGELLCGYQPLVMRDEKVFDEPEKFVVDRFTKEKGSELLSYLFWSNGPQNGSPNESNK 318
            IKKGELLCGYQPLVMRD KVFD+ E F  +RF  EKGSELLSYL+WSNGPQ G+PN+ NK
Sbjct: 400  IKKGELLCGYQPLVMRDSKVFDDAESFKAERFMGEKGSELLSYLYWSNGPQTGTPNDMNK 459

Query: 317  QCAAKDYVTLTASVLFFFFDGKY 249
            QCA KDYVTL A V+  +   +Y
Sbjct: 460  QCAGKDYVTLVACVIVAYVFQRY 482