BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00013387
         (1609 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI22695.3| unnamed protein product [Vitis vinifera]              385   e-120
ref|XP_002269721.1| PREDICTED: SKP1-like protein 21-like [Vitis ...   385   e-117
ref|XP_002513681.1| ubiquitin-protein ligase, putative [Ricinus ...   383   e-117
ref|XP_002318211.1| predicted protein [Populus trichocarpa] gi|2...   365   e-112
ref|XP_004145972.1| PREDICTED: SKP1-like protein 21-like [Cucumi...   370   e-111

>emb|CBI22695.3| unnamed protein product [Vitis vinifera]
          Length = 358

 Score =  385 bits (989), Expect(2) = e-120
 Identities = 204/296 (68%), Positives = 237/296 (80%), Gaps = 13/296 (4%)
 Frame = -3

Query: 1607 MKSYIWLQTTDGSIQQVEEEVAMFCPMICREVLQTNMGSSKNYAIKLPQRVNPAILSLIL 1428
            MKSYIWLQT DGSIQQVEEEVAMFCPMICRE+LQT MGSSKNYAI LPQRVNPAIL LIL
Sbjct: 13   MKSYIWLQTADGSIQQVEEEVAMFCPMICREILQTGMGSSKNYAISLPQRVNPAILGLIL 72

Query: 1427 EYCRFHQLPGRSNKERKTYDERFVKLDTKKLCELTSAADSLQLRPLVDLTSRALARMIEG 1248
            +YCRFHQ+PGRSNKERK++DE+F+++DTKKLCELTSAADSLQL+PLVDLTSRALAR+IEG
Sbjct: 73   DYCRFHQVPGRSNKERKSFDEKFIRMDTKKLCELTSAADSLQLKPLVDLTSRALARIIEG 132

Query: 1247 KTPEEIRETFHLPDDLTEEEKLEPLRNVTDDPRIRLLNRLYA--XXXXXXXXXXXXXXXX 1074
            KTPEEIRETFHLPDDLTEEEKLEPLRN+TDDPRIRLLNRLYA                  
Sbjct: 133  KTPEEIRETFHLPDDLTEEEKLEPLRNITDDPRIRLLNRLYARKRKELKEREKLKNVEVE 192

Query: 1073 XXXVDKRSVDDLLSFIN---GDSKSVRASKHKKKNRRRKEQLRNNSSDNGNENHE---NG 912
               VD+RSVDDLLSFIN   GDSK VRASK+KKKNRRRK+Q++++SS+N N +H+   +G
Sbjct: 193  EERVDERSVDDLLSFINGEDGDSKGVRASKNKKKNRRRKDQMKDSSSNNVNGDHKKELDG 252

Query: 911  VPSAFQNGEAED-----HSDQSAMQESTSATFSPRLEFXXXXXXXXXDPAAREELD 759
            V ++  N EA++      S  S +QE  ++TFSP+LEF         DPA +E+LD
Sbjct: 253  VHTSCHNAEADEMLVSTPSKTSKLQELPASTFSPKLEFDDGDIDDELDPAMKEQLD 308



 Score = 73.6 bits (179), Expect(2) = e-120
 Identities = 37/51 (72%), Positives = 42/51 (82%)
 Frame = -2

Query: 741 REVEDFARRLNSVWPERMQDIISLGQERTVVPFSMTNGNGVHKRSTDLDTR 589
           REVEDFARRLNS WPERMQ+I+SLGQ+R +VP SM NGNG   R T LD+R
Sbjct: 309 REVEDFARRLNSDWPERMQEILSLGQQRRLVPISM-NGNGSAHRYTSLDSR 358


>ref|XP_002269721.1| PREDICTED: SKP1-like protein 21-like [Vitis vinifera]
          Length = 359

 Score =  385 bits (989), Expect(2) = e-117
 Identities = 204/296 (68%), Positives = 237/296 (80%), Gaps = 13/296 (4%)
 Frame = -3

Query: 1607 MKSYIWLQTTDGSIQQVEEEVAMFCPMICREVLQTNMGSSKNYAIKLPQRVNPAILSLIL 1428
            MKSYIWLQT DGSIQQVEEEVAMFCPMICRE+LQT MGSSKNYAI LPQRVNPAIL LIL
Sbjct: 13   MKSYIWLQTADGSIQQVEEEVAMFCPMICREILQTGMGSSKNYAISLPQRVNPAILGLIL 72

Query: 1427 EYCRFHQLPGRSNKERKTYDERFVKLDTKKLCELTSAADSLQLRPLVDLTSRALARMIEG 1248
            +YCRFHQ+PGRSNKERK++DE+F+++DTKKLCELTSAADSLQL+PLVDLTSRALAR+IEG
Sbjct: 73   DYCRFHQVPGRSNKERKSFDEKFIRMDTKKLCELTSAADSLQLKPLVDLTSRALARIIEG 132

Query: 1247 KTPEEIRETFHLPDDLTEEEKLEPLRNVTDDPRIRLLNRLYA--XXXXXXXXXXXXXXXX 1074
            KTPEEIRETFHLPDDLTEEEKLEPLRN+TDDPRIRLLNRLYA                  
Sbjct: 133  KTPEEIRETFHLPDDLTEEEKLEPLRNITDDPRIRLLNRLYARKRKELKEREKLKNVEVE 192

Query: 1073 XXXVDKRSVDDLLSFIN---GDSKSVRASKHKKKNRRRKEQLRNNSSDNGNENHE---NG 912
               VD+RSVDDLLSFIN   GDSK VRASK+KKKNRRRK+Q++++SS+N N +H+   +G
Sbjct: 193  EERVDERSVDDLLSFINGEDGDSKGVRASKNKKKNRRRKDQMKDSSSNNVNGDHKKELDG 252

Query: 911  VPSAFQNGEAED-----HSDQSAMQESTSATFSPRLEFXXXXXXXXXDPAAREELD 759
            V ++  N EA++      S  S +QE  ++TFSP+LEF         DPA +E+LD
Sbjct: 253  VHTSCHNAEADEMLVSTPSKTSKLQELPASTFSPKLEFDDGDIDDELDPAMKEQLD 308



 Score = 66.2 bits (160), Expect(2) = e-117
 Identities = 32/40 (80%), Positives = 36/40 (90%)
 Frame = -2

Query: 741 REVEDFARRLNSVWPERMQDIISLGQERTVVPFSMTNGNG 622
           REVEDFARRLNS WPERMQ+I+SLGQ+R +VP SM NGNG
Sbjct: 309 REVEDFARRLNSDWPERMQEILSLGQQRRLVPISM-NGNG 347


>ref|XP_002513681.1| ubiquitin-protein ligase, putative [Ricinus communis]
            gi|223547589|gb|EEF49084.1| ubiquitin-protein ligase,
            putative [Ricinus communis]
          Length = 378

 Score =  383 bits (983), Expect(2) = e-117
 Identities = 204/294 (69%), Positives = 230/294 (78%), Gaps = 11/294 (3%)
 Frame = -3

Query: 1607 MKSYIWLQTTDGSIQQVEEEVAMFCPMICREVLQTNMGSSKNYAIKLPQRVNPAILSLIL 1428
            MKSYIWLQT DGSIQQVEEEVAMFCPMICRE+LQT MGSSKNYAI LPQRVNPAIL LIL
Sbjct: 1    MKSYIWLQTADGSIQQVEEEVAMFCPMICREILQTGMGSSKNYAISLPQRVNPAILGLIL 60

Query: 1427 EYCRFHQLPGRSNKERKTYDERFVKLDTKKLCELTSAADSLQLRPLVDLTSRALARMIEG 1248
            +YCRFHQ+PGRSNKERKT+DE+F+++DTK+LCELTSAADSLQL+PLVDLTSRALAR+IEG
Sbjct: 61   DYCRFHQVPGRSNKERKTFDEKFIRMDTKRLCELTSAADSLQLKPLVDLTSRALARIIEG 120

Query: 1247 KTPEEIRETFHLPDDLTEEEKLEPLRNVTDDPRIRLLNRLYA--XXXXXXXXXXXXXXXX 1074
            KTPEEIRETFHLPDDLTEEEKLEPLRNVTDDPRIRLLNRLYA                  
Sbjct: 121  KTPEEIRETFHLPDDLTEEEKLEPLRNVTDDPRIRLLNRLYARKRKELKEREKMKNVEVE 180

Query: 1073 XXXVDKRSVDDLLSFIN---GDSKSVRASKHKKKNRRRKEQLRNNSSDNGNENHE---NG 912
               VD+RSVDDLLS+IN   GD K  R SK+KKKNRRRK+Q + +SS+N NENH    N 
Sbjct: 181  EERVDERSVDDLLSYINGADGDPKGGRTSKNKKKNRRRKDQSKESSSNNLNENHNKKTNS 240

Query: 911  VPSAFQNGEAED---HSDQSAMQESTSATFSPRLEFXXXXXXXXXDPAAREELD 759
             PSA  +GE  D      +++ QES +A+ SP+LEF         DPA +EELD
Sbjct: 241  CPSACHSGEVRDMVASPSKTSRQESGAASLSPKLEFDDADIDDDLDPAMKEELD 294



 Score = 67.4 bits (163), Expect(2) = e-117
 Identities = 33/40 (82%), Positives = 36/40 (90%)
 Frame = -2

Query: 741 REVEDFARRLNSVWPERMQDIISLGQERTVVPFSMTNGNG 622
           REVEDFARRLNS WPERMQ+I+SLGQER +VP SM NGNG
Sbjct: 295 REVEDFARRLNSDWPERMQEILSLGQERRLVPLSM-NGNG 333


>ref|XP_002318211.1| predicted protein [Populus trichocarpa] gi|222858884|gb|EEE96431.1|
            predicted protein [Populus trichocarpa]
          Length = 363

 Score =  365 bits (936), Expect(2) = e-112
 Identities = 194/296 (65%), Positives = 224/296 (75%), Gaps = 13/296 (4%)
 Frame = -3

Query: 1607 MKSYIWLQTTDGSIQQVEEEVAMFCPMICREVLQTNMGSSKNYAIKLPQRVNPAILSLIL 1428
            MKSYIWLQT DGS+QQVEEEVAMFCPMICRE++Q  MGSSKN+AI LPQR+NPAIL LIL
Sbjct: 13   MKSYIWLQTADGSVQQVEEEVAMFCPMICREIIQAGMGSSKNHAISLPQRLNPAILGLIL 72

Query: 1427 EYCRFHQLPGRSNKERKTYDERFVKLDTKKLCELTSAADSLQLRPLVDLTSRALARMIEG 1248
            +YCRFHQ+PG SNKERKT+DE+F+++DTK+LCELTSAADSLQL+PLVDLTSRALAR+IEG
Sbjct: 73   DYCRFHQVPGHSNKERKTFDEKFIRMDTKRLCELTSAADSLQLKPLVDLTSRALARIIEG 132

Query: 1247 KTPEEIRETFHLPDDLTEEEKLEPLRNVTDDPRIRLLNRLYA--XXXXXXXXXXXXXXXX 1074
            KTPEEIRETFHLPDDLTEEEKLEPL N+TDDPRIRLLNRLYA                  
Sbjct: 133  KTPEEIRETFHLPDDLTEEEKLEPLGNITDDPRIRLLNRLYARKRRELREREKLKNVDVE 192

Query: 1073 XXXVDKRSVDDLLSFIN---GDSKSVRASKHKKKNRRRKEQLRNNSSDNGNENHE---NG 912
                D RSV+DLLSFIN   GDSK  R SK+KKKNRRRKEQ +++SS+N NEN +   + 
Sbjct: 193  DERSDNRSVEDLLSFINGEDGDSKGGRNSKNKKKNRRRKEQAKDSSSNNPNENQKKESDT 252

Query: 911  VPSAFQNGEAED-----HSDQSAMQESTSATFSPRLEFXXXXXXXXXDPAAREELD 759
            +PS   N E  D      S  S  Q  ++ TFSP+L+F         DPA +EELD
Sbjct: 253  LPSVSLNDEVNDILAASPSKTSNFQGCSAVTFSPKLDFDDADIDDDLDPAMKEELD 308



 Score = 67.8 bits (164), Expect(2) = e-112
 Identities = 34/51 (66%), Positives = 40/51 (78%)
 Frame = -2

Query: 741 REVEDFARRLNSVWPERMQDIISLGQERTVVPFSMTNGNGVHKRSTDLDTR 589
           REVEDFARRLNS WPERMQ+I+SL Q++  VP SM NGNG  +R + LD R
Sbjct: 309 REVEDFARRLNSDWPERMQEILSLDQKKRPVPLSM-NGNGTSRRYSGLDQR 358


>ref|XP_004145972.1| PREDICTED: SKP1-like protein 21-like [Cucumis sativus]
            gi|449524038|ref|XP_004169030.1| PREDICTED: SKP1-like
            protein 21-like [Cucumis sativus]
          Length = 425

 Score =  370 bits (950), Expect(2) = e-111
 Identities = 200/297 (67%), Positives = 228/297 (76%), Gaps = 14/297 (4%)
 Frame = -3

Query: 1607 MKSYIWLQTTDGSIQQVEEEVAMFCPMICREVLQTNMGSSKNYAIKLPQRVNPAILSLIL 1428
            MKSYIWLQT DGSIQQVEEEVAMFCPMICRE+LQT MGSSKNYAI LPQRVNPAIL LIL
Sbjct: 13   MKSYIWLQTADGSIQQVEEEVAMFCPMICREILQTGMGSSKNYAISLPQRVNPAILGLIL 72

Query: 1427 EYCRFHQLPGRSNKERKTYDERFVKLDTKKLCELTSAADSLQLRPLVDLTSRALARMIEG 1248
            +YCRFHQ+PGRSNKERKT+DE+F+++DTKKLCELTSAADSLQL+PLVDLTSRALAR+IEG
Sbjct: 73   DYCRFHQVPGRSNKERKTFDEKFIRMDTKKLCELTSAADSLQLKPLVDLTSRALARIIEG 132

Query: 1247 KTPEEIRETFHLPDDLTEEEKLEPLRNVTDDPRIRLLNRLYA-XXXXXXXXXXXXXXXXX 1071
            KTPEEIRETFHLPDDLTEEEKLEPLRN+TDDPRIRLLNRLYA                  
Sbjct: 133  KTPEEIRETFHLPDDLTEEEKLEPLRNITDDPRIRLLNRLYARKRKELREREKLKNVEIE 192

Query: 1070 XXVDKRSVDDLLSFIN---GDSKSVRASKHKKKNRRRKEQLRNNSS-DNGNENHE----N 915
              VD RSVDDLLSFIN   GDSK+V+ +K+KKKNRRRK+Q +++SS  N N NH+    +
Sbjct: 193  ERVDDRSVDDLLSFINGGDGDSKAVKTNKNKKKNRRRKDQQKDSSSTTNENGNHDKIELD 252

Query: 914  GVPSAFQNGE-----AEDHSDQSAMQESTSATFSPRLEFXXXXXXXXXDPAAREELD 759
             +PS  QN E         S     Q+S +A +S ++EF         DPA +EELD
Sbjct: 253  ALPSCCQNNEFNQILGASPSRTVKSQDSAAAMYSSKIEFDDADIYDDLDPAMKEELD 309



 Score = 59.3 bits (142), Expect(2) = e-111
 Identities = 30/46 (65%), Positives = 36/46 (78%)
 Frame = -2

Query: 741 REVEDFARRLNSVWPERMQDIISLGQERTVVPFSMTNGNGVHKRST 604
           REVEDFARRLNS WPER+Q+I+SLGQER +V  ++  GNG   R T
Sbjct: 310 REVEDFARRLNSDWPERVQEILSLGQERNLV--TVLTGNGSSPRFT 353