BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00012974
         (1752 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AGE10595.1| 4-coumarate CoA ligase [Lonicera japonica]             642   0.0  
ref|XP_002270592.1| PREDICTED: 4-coumarate--CoA ligase-like 9 [V...   613   e-173
ref|XP_002327954.1| 4-coumarate-coa ligase [Populus trichocarpa]...   600   e-169
ref|XP_002270555.1| PREDICTED: 4-coumarate--CoA ligase-like 9-li...   566   e-159
ref|XP_002509558.1| AMP dependent CoA ligase, putative [Ricinus ...   563   e-158

>gb|AGE10595.1| 4-coumarate CoA ligase [Lonicera japonica]
          Length = 540

 Score =  642 bits (1655), Expect = 0.0
 Identities = 325/465 (69%), Positives = 383/465 (82%), Gaps = 1/465 (0%)
 Frame = +1

Query: 4    TQNLASALLTRIGLTKGQTAFILSPNSTAVPVLYFALLSIGVVISPSNPISTESEISRQI 183
            T NL+S+L + +GL+KG  AFILS NS  +P+LYF+LLS+GV+ISPSNP STESEISRQI
Sbjct: 72   TNNLSSSLQSHLGLSKGDVAFILSLNSLQIPLLYFSLLSLGVIISPSNPSSTESEISRQI 131

Query: 184  KLSGPVIAFATSETCQKLPSLRHRTILLDSAEFESMMRTXXXXXXNGLGERVEVSQSDTA 363
            KLS PVIAFATS T  K+P+LRH T+L+DS EF SM  +            V VSQ D A
Sbjct: 132  KLSKPVIAFATSATSHKIPALRHGTVLIDSPEFVSMTTSSGREL-----SCVGVSQCDIA 186

Query: 364  AVLYSSGTTGQVKGVVLTHRNLIAITSSYQ-LDAERESPNVVLYTVPFFHVIGFFYCVKC 540
            A++YSSGTTGQVKGV+LTHRNL AI ++Y  L  ER+SP VVLYT+P+FHVIG F C+K 
Sbjct: 187  AIMYSSGTTGQVKGVMLTHRNLTAIVANYHALRPERKSPAVVLYTMPYFHVIGLFCCMKS 246

Query: 541  VSLSETVVVMKRFDVKKMCRAVEEHKITQLVGAPPVVVAMTKDSVTEGFDLKSLQQVGCG 720
            V+LSETVVVM+RF++K+M RAVEE ++T +  APPVVVAMTK  VT+ +DLKSL+  GCG
Sbjct: 247  VALSETVVVMERFNLKRMLRAVEELRVTNVAMAPPVVVAMTKAEVTKEYDLKSLEGFGCG 306

Query: 721  GAPLGKDMIAAFRSKFPDKEIFQGYGMTETTGAIFRSANAEESLRWGSVGKLAGHCEARI 900
            GAPLGKD+IAAF +KFP   + QGYGMTET G  FR+   EES RWGSVG+L  +CEARI
Sbjct: 307  GAPLGKDVIAAFTAKFPGVLLTQGYGMTETAGPAFRAVTPEESFRWGSVGRLQANCEARI 366

Query: 901  VDPESGNALPPGKQGELWIRGPLVMKGYIANAQATSETLVGDGWLRTGDLCYIDDEGYLF 1080
            VDP++G ALPPGKQGELW++GP+ MKGY+ + +ATSETLV DGWLRTGDLCY D+EG+LF
Sbjct: 367  VDPDTGTALPPGKQGELWLKGPITMKGYVGDPKATSETLVLDGWLRTGDLCYFDEEGFLF 426

Query: 1081 VVDRLKELIKYKGYQVPPAELEQLLQSHPEVVDAAVIPYPDEESGEVPMACVVKRHDSRI 1260
            VVDRLKELIKYKGYQVPPAELEQLLQSHPEVVDAAVIPYPD+E+G++PMA VVK   S +
Sbjct: 427  VVDRLKELIKYKGYQVPPAELEQLLQSHPEVVDAAVIPYPDDEAGQIPMAFVVKHPQSNL 486

Query: 1261 DEAQVMEFIAKQVAPYKKIRRVWFVSSIPKSAAGKILRKDLRKAA 1395
            DEAQVM F+AKQVAPYKKIRRV FVSSIPKS AGKILRK+LRK A
Sbjct: 487  DEAQVMSFVAKQVAPYKKIRRVSFVSSIPKSPAGKILRKELRKVA 531


>ref|XP_002270592.1| PREDICTED: 4-coumarate--CoA ligase-like 9 [Vitis vinifera]
          Length = 543

 Score =  613 bits (1582), Expect = e-173
 Identities = 313/473 (66%), Positives = 380/473 (80%), Gaps = 1/473 (0%)
 Frame = +1

Query: 4    TQNLASALLTRIGLTKGQTAFILSPNSTAVPVLYFALLSIGVVISPSNPISTESEISRQI 183
            ++ LA+ L T IGL+KG TAF+LS N   VP+LYF+LLS+GV+ISP+NPI+T+SEISR I
Sbjct: 76   SKTLAAYLQTVIGLSKGDTAFVLSSNLIQVPILYFSLLSLGVIISPANPINTQSEISRLI 135

Query: 184  KLSGPVIAFATSETCQKLPSLRHRTILLDSAEFESMMRTXXXXXXNGLGERVEVSQSDTA 363
            +L  PVIAF+ S    KLPSL HRT+++DS EF+SMM              VEVSQSD A
Sbjct: 136  ELCKPVIAFSISTAAHKLPSLHHRTVIIDSFEFDSMMTGPIRELVP-----VEVSQSDLA 190

Query: 364  AVLYSSGTTGQVKGVVLTHRNLIAITSS-YQLDAERESPNVVLYTVPFFHVIGFFYCVKC 540
            A++YSSGTTG+VKGV+LTHRNLIA+T+S ++  + R SP+V+LYTVPFFH+IGFFYCVK 
Sbjct: 191  AIMYSSGTTGRVKGVMLTHRNLIAMTASIHEGQSVRSSPDVLLYTVPFFHMIGFFYCVKS 250

Query: 541  VSLSETVVVMKRFDVKKMCRAVEEHKITQLVGAPPVVVAMTKDSVTEGFDLKSLQQVGCG 720
            V+L+ETVVVM+RFD+++M   VE+ K+T +   PPV+VAM K   T+  DL SL+ V CG
Sbjct: 251  VALNETVVVMERFDLRRMLTLVEKFKVTHMAVVPPVLVAMAKGDATDNNDLTSLESVSCG 310

Query: 721  GAPLGKDMIAAFRSKFPDKEIFQGYGMTETTGAIFRSANAEESLRWGSVGKLAGHCEARI 900
            GAPLGKD+  AF +KFP+  I QGYG+TE++GA+ R  + EE   WGSVG+L G  EA+I
Sbjct: 311  GAPLGKDLCQAFTAKFPNVAIRQGYGLTESSGAVSRLLDPEECRHWGSVGRLTGIFEAKI 370

Query: 901  VDPESGNALPPGKQGELWIRGPLVMKGYIANAQATSETLVGDGWLRTGDLCYIDDEGYLF 1080
            VDP++G ALPP KQGELW+RGP VMKGY+ + +AT ETLV DGWLRTGDLCYID EG+L+
Sbjct: 371  VDPDTGLALPPCKQGELWVRGPAVMKGYVGDRKATRETLVSDGWLRTGDLCYIDGEGFLY 430

Query: 1081 VVDRLKELIKYKGYQVPPAELEQLLQSHPEVVDAAVIPYPDEESGEVPMACVVKRHDSRI 1260
            VVDRLKELIKYKGYQV PAELE LLQSHPE+VDAAVIPYPDEE+GEVPMA VV+R  S +
Sbjct: 431  VVDRLKELIKYKGYQVAPAELEHLLQSHPEIVDAAVIPYPDEEAGEVPMAYVVRRPQSSL 490

Query: 1261 DEAQVMEFIAKQVAPYKKIRRVWFVSSIPKSAAGKILRKDLRKAAAIGSLSKL 1419
            +EAQVM+FIAKQVA YKKIRRV FVSSIPKS AGKILRK+LRK A  GSLSKL
Sbjct: 491  NEAQVMDFIAKQVAAYKKIRRVLFVSSIPKSPAGKILRKELRKLAVPGSLSKL 543


>ref|XP_002327954.1| 4-coumarate-coa ligase [Populus trichocarpa]
            gi|224133074|ref|XP_002327955.1| acyl:coa ligase [Populus
            trichocarpa] gi|222837363|gb|EEE75742.1| 4-coumarate-coa
            ligase [Populus trichocarpa] gi|222837364|gb|EEE75743.1|
            acyl:coa ligase [Populus trichocarpa]
          Length = 548

 Score =  600 bits (1548), Expect = e-169
 Identities = 300/448 (66%), Positives = 364/448 (81%), Gaps = 3/448 (0%)
 Frame = +1

Query: 43   LTKGQTAFILSPNSTAVPVLYFALLSIGVVISPSNPISTESEISRQIKLSGPVIAFATSE 222
            L K  TAFIL PNS  VP+LYF+LLS+GVVISP+NPISTESEIS QI LS PVIAFA S+
Sbjct: 91   LRKNDTAFILCPNSIQVPILYFSLLSLGVVISPANPISTESEISHQISLSNPVIAFAISK 150

Query: 223  TCQKLPSLRHRTILLDSAEFESM--MRTXXXXXXNGLGERVEVSQSDTAAVLYSSGTTGQ 396
            TC KLP L+H TIL+DS EF+S+  M          L E+V V+QSD AA+++SSGTTG+
Sbjct: 151  TCHKLPKLKHGTILIDSPEFDSIITMSPATTTARQDL-EKVTVNQSDLAAIMFSSGTTGK 209

Query: 397  VKGVVLTHRNLIAITSSYQ-LDAERESPNVVLYTVPFFHVIGFFYCVKCVSLSETVVVMK 573
            VKGV+LTHRNL+A+ + Y     ER+SP V+LYTVP+FHV GFFY  K V+LSETVVVM+
Sbjct: 210  VKGVMLTHRNLMAVIAGYYPFKQERKSPTVMLYTVPYFHVFGFFYSFKSVALSETVVVME 269

Query: 574  RFDVKKMCRAVEEHKITQLVGAPPVVVAMTKDSVTEGFDLKSLQQVGCGGAPLGKDMIAA 753
            RFD+KKM RAVE+ ++T L  APPVVVAM K  +T+G+DL+SL+ VGCGGAPLGKD++  
Sbjct: 270  RFDLKKMLRAVEKFRVTHLAVAPPVVVAMAKSDLTDGYDLRSLETVGCGGAPLGKDVMKV 329

Query: 754  FRSKFPDKEIFQGYGMTETTGAIFRSANAEESLRWGSVGKLAGHCEARIVDPESGNALPP 933
            F  +FP  +++QGYG+TE+TG + RS + EES  WGSVG+L   CEA+IVD ++G+ALPP
Sbjct: 330  FADRFPTVDLWQGYGLTESTGVLSRSNSPEESRHWGSVGRLTACCEAKIVDADTGDALPP 389

Query: 934  GKQGELWIRGPLVMKGYIANAQATSETLVGDGWLRTGDLCYIDDEGYLFVVDRLKELIKY 1113
            GKQGELW+RG  +MKGY+ + +ATS TL  DGWLRTGDLCYID+EG+LFVVDRLKELIKY
Sbjct: 390  GKQGELWVRGSTIMKGYVGDPEATSTTLDPDGWLRTGDLCYIDEEGFLFVVDRLKELIKY 449

Query: 1114 KGYQVPPAELEQLLQSHPEVVDAAVIPYPDEESGEVPMACVVKRHDSRIDEAQVMEFIAK 1293
            KGYQV PAELEQLL SHPE+ DAAVIPYPDEE+G+VPMA VVK+  SRI+E  VM+F+AK
Sbjct: 450  KGYQVAPAELEQLLHSHPEIADAAVIPYPDEEAGQVPMAFVVKQPQSRINERGVMDFVAK 509

Query: 1294 QVAPYKKIRRVWFVSSIPKSAAGKILRK 1377
            QVAPYKK+RRV FVS+IPKS AGK LRK
Sbjct: 510  QVAPYKKVRRVEFVSAIPKSPAGKELRK 537


>ref|XP_002270555.1| PREDICTED: 4-coumarate--CoA ligase-like 9-like [Vitis vinifera]
          Length = 541

 Score =  566 bits (1459), Expect = e-159
 Identities = 285/472 (60%), Positives = 368/472 (77%)
 Frame = +1

Query: 4    TQNLASALLTRIGLTKGQTAFILSPNSTAVPVLYFALLSIGVVISPSNPISTESEISRQI 183
            ++ LA+ L + +GL KG  A+ILS N   VPVLYFALLS+GVV+SP+NPI+T++E++RQ 
Sbjct: 76   SKTLAAYLQSIVGLNKGDAAYILSNNLIQVPVLYFALLSLGVVVSPANPINTKAEVARQT 135

Query: 184  KLSGPVIAFATSETCQKLPSLRHRTILLDSAEFESMMRTXXXXXXNGLGERVEVSQSDTA 363
            +L  PVIAF  S    K+P LR+ TI++DS EFE MM +      +     V+VSQSD A
Sbjct: 136  QLCRPVIAFTISTAVHKIPKLRYGTIVIDSFEFELMMTSPRREMVD-----VKVSQSDLA 190

Query: 364  AVLYSSGTTGQVKGVVLTHRNLIAITSSYQLDAERESPNVVLYTVPFFHVIGFFYCVKCV 543
             ++YSSGTTG VKGV++THRNLIA+T SY +  +  SP V+L  VP+FHV GF Y  KC+
Sbjct: 191  GIMYSSGTTGNVKGVMVTHRNLIAMTGSY-MQRKANSPVVLLQIVPYFHVYGFHYVFKCM 249

Query: 544  SLSETVVVMKRFDVKKMCRAVEEHKITQLVGAPPVVVAMTKDSVTEGFDLKSLQQVGCGG 723
            +++ TVV+M+++D++K   AVE++K+T L  APPVVVAM+K +VTEG DL SL+ V  GG
Sbjct: 250  AMNVTVVIMEKYDLEKTIDAVEKYKVTDLAVAPPVVVAMSKKAVTEGRDLSSLETVASGG 309

Query: 724  APLGKDMIAAFRSKFPDKEIFQGYGMTETTGAIFRSANAEESLRWGSVGKLAGHCEARIV 903
            APLGK++I AF +KFP   I QGYGMTE  G I  + + EE  RWGS GK  G  EA+IV
Sbjct: 310  APLGKELIEAFTAKFPGTVISQGYGMTEVIGRISEALDREECSRWGSSGKFPGIWEAKIV 369

Query: 904  DPESGNALPPGKQGELWIRGPLVMKGYIANAQATSETLVGDGWLRTGDLCYIDDEGYLFV 1083
            D E+G +LPP K+GELW++GP +MKGY+ + +ATSETL  DGWLRTGDLCYID++G+LF+
Sbjct: 370  DQETGASLPPLKRGELWVKGPSIMKGYVNDPKATSETLTPDGWLRTGDLCYIDEDGFLFI 429

Query: 1084 VDRLKELIKYKGYQVPPAELEQLLQSHPEVVDAAVIPYPDEESGEVPMACVVKRHDSRID 1263
            VDRLKELIKYKGYQV PAELE LLQSHP++VDAAVIPYPD+E+G+VPMA +V+R +S++D
Sbjct: 430  VDRLKELIKYKGYQVAPAELEHLLQSHPQIVDAAVIPYPDDEAGQVPMAFIVRRPESKLD 489

Query: 1264 EAQVMEFIAKQVAPYKKIRRVWFVSSIPKSAAGKILRKDLRKAAAIGSLSKL 1419
            EAQVM+FIAKQVAPYKKIRRV FV+SIPK+A+GKILRK+L+K +   S  KL
Sbjct: 490  EAQVMDFIAKQVAPYKKIRRVSFVTSIPKNASGKILRKELKKISLPESFPKL 541


>ref|XP_002509558.1| AMP dependent CoA ligase, putative [Ricinus communis]
            gi|223549457|gb|EEF50945.1| AMP dependent CoA ligase,
            putative [Ricinus communis]
          Length = 540

 Score =  563 bits (1451), Expect = e-158
 Identities = 277/461 (60%), Positives = 357/461 (77%)
 Frame = +1

Query: 4    TQNLASALLTRIGLTKGQTAFILSPNSTAVPVLYFALLSIGVVISPSNPISTESEISRQI 183
            T++L   L   I L+K   AF+L P ST +P+LYF+LLS+GV+ISPSNP++TE EISRQI
Sbjct: 74   TKSLTCYLHNVIKLSKNDVAFVLCPKSTKIPILYFSLLSLGVIISPSNPLNTELEISRQI 133

Query: 184  KLSGPVIAFATSETCQKLPSLRHRTILLDSAEFESMMRTXXXXXXNGLGERVEVSQSDTA 363
             LS PVIAFATS T  K+P L H+TILLDS EF S++ T          ++V V QSD A
Sbjct: 134  SLSKPVIAFATSSTAHKIPKLNHKTILLDSPEFNSILTTSCPTHEL---DKVTVRQSDLA 190

Query: 364  AVLYSSGTTGQVKGVVLTHRNLIAITSSYQLDAERESPNVVLYTVPFFHVIGFFYCVKCV 543
             + YSSGTTG+VK V+LTHRNL A+   Y   A ++   V L  VP+FHV GFFY +K V
Sbjct: 191  GIFYSSGTTGKVKAVMLTHRNLTAVVGGYL--ALKQEKAVTLNPVPYFHVYGFFYSLKSV 248

Query: 544  SLSETVVVMKRFDVKKMCRAVEEHKITQLVGAPPVVVAMTKDSVTEGFDLKSLQQVGCGG 723
            +L E VVVM++F++KKM RAV+E ++TQL  APP+VV M KD +T+ +DL+SLQ VGCG 
Sbjct: 249  ALREVVVVMEKFELKKMLRAVQEFRVTQLAVAPPIVVQMVKDGLTDCYDLRSLQAVGCGA 308

Query: 724  APLGKDMIAAFRSKFPDKEIFQGYGMTETTGAIFRSANAEESLRWGSVGKLAGHCEARIV 903
            APLG+D+IAAF+++FP  E++QGYG+TE++G   R+    ES  WGSVG+L  +C+ +IV
Sbjct: 309  APLGRDVIAAFKARFPTVELWQGYGLTESSGVCSRATGPGESHCWGSVGRLTAYCKVKIV 368

Query: 904  DPESGNALPPGKQGELWIRGPLVMKGYIANAQATSETLVGDGWLRTGDLCYIDDEGYLFV 1083
            +P++  AL PGKQGELWI+GP +MKGY+ + +ATS  L  DGWLRTGDLCYID+EG++F+
Sbjct: 369  NPDTMVALLPGKQGELWIKGPTIMKGYVGDPEATSAALTSDGWLRTGDLCYIDEEGFVFI 428

Query: 1084 VDRLKELIKYKGYQVPPAELEQLLQSHPEVVDAAVIPYPDEESGEVPMACVVKRHDSRID 1263
            VDRLKELIKYKGYQV PAELEQLL SHPE+ DAAVIPYP+EE+G++P+A +VK+  S ++
Sbjct: 429  VDRLKELIKYKGYQVAPAELEQLLLSHPEIADAAVIPYPNEEAGQIPIAFIVKQPQSSLN 488

Query: 1264 EAQVMEFIAKQVAPYKKIRRVWFVSSIPKSAAGKILRKDLR 1386
            E  +M+F+AKQVAPYKKIRRV FV+SIPKS +GKILRKDLR
Sbjct: 489  EKDIMDFVAKQVAPYKKIRRVAFVNSIPKSPSGKILRKDLR 529