BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00012903
         (1582 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CAC00547.1| gamma tubulin [Nicotiana tabacum]                     486   0.0  
gb|ABF19052.1| gamma-tubulin, partial [Nicotiana tabacum]             486   0.0  
ref|XP_002276643.1| PREDICTED: tubulin gamma-1 chain [Vitis vini...   492   0.0  
ref|XP_004133834.1| PREDICTED: tubulin gamma-2 chain-like [Cucum...   488   0.0  
dbj|BAB18571.1| gamma-tubulin1 [Nicotiana tabacum]                    481   0.0  

>emb|CAC00547.1| gamma tubulin [Nicotiana tabacum]
          Length = 474

 Score =  486 bits (1252), Expect(2) = 0.0
 Identities = 242/266 (90%), Positives = 255/266 (95%)
 Frame = -2

Query: 1035 ALNRIAVERLHIANPSVSQTNSLVSTVMSASTTTLRYPGYMNNDLVGLLASLIPTPRCHF 856
            ALNRIAVERLHI  P+ +QTNSLVSTVMSASTTTLRYPGYMNNDLVGLLASLIPTPRCHF
Sbjct: 209  ALNRIAVERLHITTPTFAQTNSLVSTVMSASTTTLRYPGYMNNDLVGLLASLIPTPRCHF 268

Query: 855  LMTGYTPLTVERQANMIRKTTVLDVMRRLLQTKNIMVSSNARTKEASQAKYISILNIIQG 676
            LMTGYTPLTVERQAN+IRKTTVLDVMRRLLQTKNIMVSS ARTKEASQAKYISILNIIQG
Sbjct: 269  LMTGYTPLTVERQANVIRKTTVLDVMRRLLQTKNIMVSSYARTKEASQAKYISILNIIQG 328

Query: 675  EVDPTQVHDSLQRIRERKLVNFIEWGPASIQVALSRKSPYVQTAHRVSGLMLASYTGIRH 496
            EVDPTQVH+SLQRIRERKLVNFI+WGPASIQVALSRKSPYVQTAHRVSGLMLAS+TGIRH
Sbjct: 329  EVDPTQVHESLQRIRERKLVNFIDWGPASIQVALSRKSPYVQTAHRVSGLMLASHTGIRH 388

Query: 495  LFSKCLSQYSLLRKRQAFLDKYRSFPLFDDNDLSEFDESRDIIESLVDEYKACESPDYIK 316
            LFSKCLSQY  LRKRQAFLD YR+ P F DNDLSEFDESRD+IESLVDEYKACESPDYIK
Sbjct: 389  LFSKCLSQYDKLRKRQAFLDNYRNHPTFADNDLSEFDESRDVIESLVDEYKACESPDYIK 448

Query: 315  WGMEDPDHILTGEGNATGSVDPNLAI 238
            WGMEDPDH+LTGEGNA+G+VDP L++
Sbjct: 449  WGMEDPDHVLTGEGNASGTVDPKLSL 474



 Score =  295 bits (756), Expect(2) = 0.0
 Identities = 140/154 (90%), Positives = 148/154 (96%)
 Frame = -1

Query: 1582 GISKDGILEDFATQGGDRKDVFFYQADDQHYIPRALLMDLEPRVINGIQTGDYRNLFNQE 1403
            GISK+GILEDFATQGGDRKDVFFYQADDQHYIPRALLMDLEPRVINGIQ G+YRNL+N E
Sbjct: 30   GISKEGILEDFATQGGDRKDVFFYQADDQHYIPRALLMDLEPRVINGIQNGEYRNLYNHE 89

Query: 1402 NLFMSKEGGGAGNNWASGYHQGKSFEEDLMDMIDREADGSDSLEGFVLCHSIAGGTGSGM 1223
            N+F++  GGGAGNNWASGYHQGK +EEDLMDMIDREADGSDSLEGFVLCHSIAGGTGSGM
Sbjct: 90   NVFIADHGGGAGNNWASGYHQGKQYEEDLMDMIDREADGSDSLEGFVLCHSIAGGTGSGM 149

Query: 1222 GSYLLETLNDRYSKKLVQTYSVFPNQNEISDVVV 1121
            GSY+LETLNDRYSKKLVQTYSVFPNQNE SDVVV
Sbjct: 150  GSYILETLNDRYSKKLVQTYSVFPNQNETSDVVV 183


>gb|ABF19052.1| gamma-tubulin, partial [Nicotiana tabacum]
          Length = 464

 Score =  486 bits (1252), Expect(2) = 0.0
 Identities = 242/266 (90%), Positives = 255/266 (95%)
 Frame = -2

Query: 1035 ALNRIAVERLHIANPSVSQTNSLVSTVMSASTTTLRYPGYMNNDLVGLLASLIPTPRCHF 856
            ALNRIAVERLHI  P+ +QTNSLVSTVMSASTTTLRYPGYMNNDLVGLLASLIPTPRCHF
Sbjct: 199  ALNRIAVERLHITTPTFAQTNSLVSTVMSASTTTLRYPGYMNNDLVGLLASLIPTPRCHF 258

Query: 855  LMTGYTPLTVERQANMIRKTTVLDVMRRLLQTKNIMVSSNARTKEASQAKYISILNIIQG 676
            LMTGYTPLTVERQAN+IRKTTVLDVMRRLLQTKNIMVSS ARTKEASQAKYISILNIIQG
Sbjct: 259  LMTGYTPLTVERQANVIRKTTVLDVMRRLLQTKNIMVSSYARTKEASQAKYISILNIIQG 318

Query: 675  EVDPTQVHDSLQRIRERKLVNFIEWGPASIQVALSRKSPYVQTAHRVSGLMLASYTGIRH 496
            EVDPTQVH+SLQRIRERKLVNFI+WGPASIQVALSRKSPYVQTAHRVSGLMLAS+TGIRH
Sbjct: 319  EVDPTQVHESLQRIRERKLVNFIDWGPASIQVALSRKSPYVQTAHRVSGLMLASHTGIRH 378

Query: 495  LFSKCLSQYSLLRKRQAFLDKYRSFPLFDDNDLSEFDESRDIIESLVDEYKACESPDYIK 316
            LFSKCLSQY  LRKRQAFLD YR+ P F DNDLSEFDESRD+IESLVDEYKACESPDYIK
Sbjct: 379  LFSKCLSQYDKLRKRQAFLDNYRNHPTFADNDLSEFDESRDVIESLVDEYKACESPDYIK 438

Query: 315  WGMEDPDHILTGEGNATGSVDPNLAI 238
            WGMEDPDH+LTGEGNA+G+VDP L++
Sbjct: 439  WGMEDPDHVLTGEGNASGTVDPKLSL 464



 Score =  295 bits (756), Expect(2) = 0.0
 Identities = 140/154 (90%), Positives = 148/154 (96%)
 Frame = -1

Query: 1582 GISKDGILEDFATQGGDRKDVFFYQADDQHYIPRALLMDLEPRVINGIQTGDYRNLFNQE 1403
            GISK+GILEDFATQGGDRKDVFFYQADDQHYIPRALLMDLEPRVINGIQ G+YRNL+N E
Sbjct: 20   GISKEGILEDFATQGGDRKDVFFYQADDQHYIPRALLMDLEPRVINGIQNGEYRNLYNHE 79

Query: 1402 NLFMSKEGGGAGNNWASGYHQGKSFEEDLMDMIDREADGSDSLEGFVLCHSIAGGTGSGM 1223
            N+F++  GGGAGNNWASGYHQGK +EEDLMDMIDREADGSDSLEGFVLCHSIAGGTGSGM
Sbjct: 80   NVFIADHGGGAGNNWASGYHQGKQYEEDLMDMIDREADGSDSLEGFVLCHSIAGGTGSGM 139

Query: 1222 GSYLLETLNDRYSKKLVQTYSVFPNQNEISDVVV 1121
            GSY+LETLNDRYSKKLVQTYSVFPNQNE SDVVV
Sbjct: 140  GSYILETLNDRYSKKLVQTYSVFPNQNETSDVVV 173


>ref|XP_002276643.1| PREDICTED: tubulin gamma-1 chain [Vitis vinifera]
            gi|297745396|emb|CBI40476.3| unnamed protein product
            [Vitis vinifera]
          Length = 474

 Score =  492 bits (1266), Expect(2) = 0.0
 Identities = 245/266 (92%), Positives = 257/266 (96%)
 Frame = -2

Query: 1035 ALNRIAVERLHIANPSVSQTNSLVSTVMSASTTTLRYPGYMNNDLVGLLASLIPTPRCHF 856
            ALNRIAVERLH++NP+ +QTNSLVSTVMSASTTTLRYPGYMNNDLVGLLASLIPTPRCHF
Sbjct: 209  ALNRIAVERLHLSNPTFAQTNSLVSTVMSASTTTLRYPGYMNNDLVGLLASLIPTPRCHF 268

Query: 855  LMTGYTPLTVERQANMIRKTTVLDVMRRLLQTKNIMVSSNARTKEASQAKYISILNIIQG 676
            LMTGYTPLTVERQAN+IRKTTVLDVMRRLLQTKNIMVSS ARTKEASQAKYISILNIIQG
Sbjct: 269  LMTGYTPLTVERQANVIRKTTVLDVMRRLLQTKNIMVSSYARTKEASQAKYISILNIIQG 328

Query: 675  EVDPTQVHDSLQRIRERKLVNFIEWGPASIQVALSRKSPYVQTAHRVSGLMLASYTGIRH 496
            EVDPTQVH+SLQRIRERKLVNFIEWGPASIQVALSRKSPYVQTAHRVSGLMLAS+T IRH
Sbjct: 329  EVDPTQVHESLQRIRERKLVNFIEWGPASIQVALSRKSPYVQTAHRVSGLMLASHTSIRH 388

Query: 495  LFSKCLSQYSLLRKRQAFLDKYRSFPLFDDNDLSEFDESRDIIESLVDEYKACESPDYIK 316
            LFSKCLSQY  LRK+QAFLD YR FP+F DNDLSEFDESRDIIESLVDEYKACESPDYIK
Sbjct: 389  LFSKCLSQYEKLRKKQAFLDNYRKFPMFADNDLSEFDESRDIIESLVDEYKACESPDYIK 448

Query: 315  WGMEDPDHILTGEGNATGSVDPNLAI 238
            WGMEDPDHILTGEGNA+G+VDP LA+
Sbjct: 449  WGMEDPDHILTGEGNASGAVDPKLAV 474



 Score =  288 bits (738), Expect(2) = 0.0
 Identities = 138/154 (89%), Positives = 145/154 (94%)
 Frame = -1

Query: 1582 GISKDGILEDFATQGGDRKDVFFYQADDQHYIPRALLMDLEPRVINGIQTGDYRNLFNQE 1403
            GISK+GILEDFATQGGDRKDVFFYQADDQHYIPRALL+DLEPRVINGIQ  +YRNL+N E
Sbjct: 30   GISKEGILEDFATQGGDRKDVFFYQADDQHYIPRALLIDLEPRVINGIQNSEYRNLYNHE 89

Query: 1402 NLFMSKEGGGAGNNWASGYHQGKSFEEDLMDMIDREADGSDSLEGFVLCHSIAGGTGSGM 1223
            N+F+S  GGGAGNNWASGYHQGK  EED+MDMIDREADGSDSLEGFVLCHSIAGGTGSGM
Sbjct: 90   NIFVSDHGGGAGNNWASGYHQGKGVEEDIMDMIDREADGSDSLEGFVLCHSIAGGTGSGM 149

Query: 1222 GSYLLETLNDRYSKKLVQTYSVFPNQNEISDVVV 1121
            GSYLLETLNDRYSKKLVQTYSVFPNQ E SDVVV
Sbjct: 150  GSYLLETLNDRYSKKLVQTYSVFPNQMETSDVVV 183


>ref|XP_004133834.1| PREDICTED: tubulin gamma-2 chain-like [Cucumis sativus]
            gi|449480269|ref|XP_004155846.1| PREDICTED: tubulin
            gamma-2 chain-like [Cucumis sativus]
          Length = 474

 Score =  488 bits (1255), Expect(2) = 0.0
 Identities = 243/266 (91%), Positives = 256/266 (96%)
 Frame = -2

Query: 1035 ALNRIAVERLHIANPSVSQTNSLVSTVMSASTTTLRYPGYMNNDLVGLLASLIPTPRCHF 856
            ALNRIAVERLH++NP+ +QTNSLVSTVMSASTTTLRYPGYMNNDLVGLLASLIPTPRCHF
Sbjct: 209  ALNRIAVERLHLSNPTFAQTNSLVSTVMSASTTTLRYPGYMNNDLVGLLASLIPTPRCHF 268

Query: 855  LMTGYTPLTVERQANMIRKTTVLDVMRRLLQTKNIMVSSNARTKEASQAKYISILNIIQG 676
            LMTGYTPLTVERQAN+IRKTTVLDVMRRLLQTKNIMVSS ARTKEASQAKYISILNIIQG
Sbjct: 269  LMTGYTPLTVERQANVIRKTTVLDVMRRLLQTKNIMVSSYARTKEASQAKYISILNIIQG 328

Query: 675  EVDPTQVHDSLQRIRERKLVNFIEWGPASIQVALSRKSPYVQTAHRVSGLMLASYTGIRH 496
            EVDPTQVH+SLQRIRERKLVNFIEWGPASIQVALSRKSPYVQTAHRVSGLMLAS+T IRH
Sbjct: 329  EVDPTQVHESLQRIRERKLVNFIEWGPASIQVALSRKSPYVQTAHRVSGLMLASHTSIRH 388

Query: 495  LFSKCLSQYSLLRKRQAFLDKYRSFPLFDDNDLSEFDESRDIIESLVDEYKACESPDYIK 316
            LFSKCLSQY  LRK+QAFLD YR FP+F DNDLSEFDESRDIIESLVDEYKACESPDYIK
Sbjct: 389  LFSKCLSQYEKLRKKQAFLDNYRKFPMFADNDLSEFDESRDIIESLVDEYKACESPDYIK 448

Query: 315  WGMEDPDHILTGEGNATGSVDPNLAI 238
            WGMEDPD ILTGEGNA+G+ DP+LA+
Sbjct: 449  WGMEDPDQILTGEGNASGTADPSLAV 474



 Score =  290 bits (742), Expect(2) = 0.0
 Identities = 139/154 (90%), Positives = 145/154 (94%)
 Frame = -1

Query: 1582 GISKDGILEDFATQGGDRKDVFFYQADDQHYIPRALLMDLEPRVINGIQTGDYRNLFNQE 1403
            GISKDGILEDFATQGGDRKDVFFYQADDQHYIPRALL+DLEPRVINGIQ  +YRNL+N E
Sbjct: 30   GISKDGILEDFATQGGDRKDVFFYQADDQHYIPRALLIDLEPRVINGIQNSEYRNLYNHE 89

Query: 1402 NLFMSKEGGGAGNNWASGYHQGKSFEEDLMDMIDREADGSDSLEGFVLCHSIAGGTGSGM 1223
            N+F+S  GGGAGNNWASGYHQGK  EED+MDMIDREADGSDSLEGFVLCHSIAGGTGSGM
Sbjct: 90   NIFVSDHGGGAGNNWASGYHQGKGVEEDIMDMIDREADGSDSLEGFVLCHSIAGGTGSGM 149

Query: 1222 GSYLLETLNDRYSKKLVQTYSVFPNQNEISDVVV 1121
            GSYLLETLNDRYSKKLVQTYSVFPNQ E SDVVV
Sbjct: 150  GSYLLETLNDRYSKKLVQTYSVFPNQMETSDVVV 183


>dbj|BAB18571.1| gamma-tubulin1 [Nicotiana tabacum]
          Length = 474

 Score =  481 bits (1238), Expect(2) = 0.0
 Identities = 241/266 (90%), Positives = 254/266 (95%)
 Frame = -2

Query: 1035 ALNRIAVERLHIANPSVSQTNSLVSTVMSASTTTLRYPGYMNNDLVGLLASLIPTPRCHF 856
            ALNRIAVERLHI  P+ +QTNSLVSTVMSASTTTLRYPGYMNNDLVGLLASLIPTPRCHF
Sbjct: 209  ALNRIAVERLHITTPTFAQTNSLVSTVMSASTTTLRYPGYMNNDLVGLLASLIPTPRCHF 268

Query: 855  LMTGYTPLTVERQANMIRKTTVLDVMRRLLQTKNIMVSSNARTKEASQAKYISILNIIQG 676
            LMTGYTPLTVERQAN+IRKTTVLDVMRRLLQTKNIMVSS ARTKEASQAKYISILNIIQG
Sbjct: 269  LMTGYTPLTVERQANVIRKTTVLDVMRRLLQTKNIMVSSYARTKEASQAKYISILNIIQG 328

Query: 675  EVDPTQVHDSLQRIRERKLVNFIEWGPASIQVALSRKSPYVQTAHRVSGLMLASYTGIRH 496
            EVDPTQVH+SLQRIRERKLVNFI+WGPASIQVALSRKSPYVQTAHRVSGLMLAS+TGIRH
Sbjct: 329  EVDPTQVHESLQRIRERKLVNFIDWGPASIQVALSRKSPYVQTAHRVSGLMLASHTGIRH 388

Query: 495  LFSKCLSQYSLLRKRQAFLDKYRSFPLFDDNDLSEFDESRDIIESLVDEYKACESPDYIK 316
            LFSKCLSQY  LRKRQAFLD YR+ P F DNDLSEFDESRD+IESLVDEYKACESPDYIK
Sbjct: 389  LFSKCLSQYDKLRKRQAFLDNYRNHPTFADNDLSEFDESRDVIESLVDEYKACESPDYIK 448

Query: 315  WGMEDPDHILTGEGNATGSVDPNLAI 238
             GMEDPDH+LTGEGNA+G+VDP L++
Sbjct: 449  RGMEDPDHVLTGEGNASGTVDPKLSL 474



 Score =  295 bits (756), Expect(2) = 0.0
 Identities = 140/154 (90%), Positives = 148/154 (96%)
 Frame = -1

Query: 1582 GISKDGILEDFATQGGDRKDVFFYQADDQHYIPRALLMDLEPRVINGIQTGDYRNLFNQE 1403
            GISK+GILEDFATQGGDRKDVFFYQADDQHYIPRALLMDLEPRVINGIQ G+YRNL+N E
Sbjct: 30   GISKEGILEDFATQGGDRKDVFFYQADDQHYIPRALLMDLEPRVINGIQNGEYRNLYNHE 89

Query: 1402 NLFMSKEGGGAGNNWASGYHQGKSFEEDLMDMIDREADGSDSLEGFVLCHSIAGGTGSGM 1223
            N+F++  GGGAGNNWASGYHQGK +EEDLMDMIDREADGSDSLEGFVLCHSIAGGTGSGM
Sbjct: 90   NVFIADHGGGAGNNWASGYHQGKQYEEDLMDMIDREADGSDSLEGFVLCHSIAGGTGSGM 149

Query: 1222 GSYLLETLNDRYSKKLVQTYSVFPNQNEISDVVV 1121
            GSY+LETLNDRYSKKLVQTYSVFPNQNE SDVVV
Sbjct: 150  GSYILETLNDRYSKKLVQTYSVFPNQNETSDVVV 183