BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00012821
         (1177 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002277768.1| PREDICTED: alpha-ketoglutarate-dependent dio...   451   e-124
emb|CAN72697.1| hypothetical protein VITISV_011566 [Vitis vinifera]   450   e-124
ref|XP_002304683.1| predicted protein [Populus trichocarpa] gi|2...   444   e-122
gb|AEL99108.1| alpha-ketoglutarate-dependent dioxygenase alkB, p...   429   e-118
ref|XP_004135327.1| PREDICTED: alpha-ketoglutarate-dependent dio...   427   e-117

>ref|XP_002277768.1| PREDICTED: alpha-ketoglutarate-dependent dioxygenase alkB [Vitis
            vinifera] gi|297738457|emb|CBI27658.3| unnamed protein
            product [Vitis vinifera]
          Length = 360

 Score =  451 bits (1161), Expect = e-124
 Identities = 223/327 (68%), Positives = 263/327 (80%), Gaps = 1/327 (0%)
 Frame = -1

Query: 1033 SASTGRKKQAIPVDLTQVLDFNSTLQSFNSNGELPPGVSLAAHQFDRPVFCLDEHPGFYF 854
            S S+ +KKQ   VDL++V+DF + L+SFN +GE+PPG       FDRPVFC++  PGFYF
Sbjct: 30   SKSSKKKKQLKQVDLSEVVDFKAILRSFNQSGEVPPGTFALQCDFDRPVFCIENRPGFYF 89

Query: 853  IPEALSVTEQCHWIKESLTTFPQPPNRTNHNAAYGPIHDLFIAAIQNKVLVENQNLSEGL 674
            IP+AL+V EQ  WI+ESL +FPQP NRTNHNA YGPI DLF+AA + K+LVE ++ S GL
Sbjct: 90   IPDALTVEEQGRWIRESLISFPQPYNRTNHNAIYGPIRDLFMAAKERKILVEEEDSSGGL 149

Query: 673  LPDDKTRATEL-AYTWNSSEESIASLRESTCKSVSASTLLRKLRWTTLGLQFDWSQRSYN 497
                 +  T + A  W   EE+  S R STCKS+SAS LLRKLRW+TLGLQFDWS+R+YN
Sbjct: 150  DSLSNSSITNMDAERWKFYEENTVSSRGSTCKSISASVLLRKLRWSTLGLQFDWSKRNYN 209

Query: 496  VGLPHNKIPDRLCQLAKKMAAPAMSLGEEFQPEAAIVNYFREGDMLGGHLDDMEADWSKP 317
            V LPHNKIPD LC LAK+MAAPAMS G+ FQPEAAIVNYF  GDMLGGHLDDMEADW+KP
Sbjct: 210  VSLPHNKIPDALCDLAKEMAAPAMSKGKVFQPEAAIVNYFGLGDMLGGHLDDMEADWTKP 269

Query: 316  IVSMSLGCKAIFLLGGKSREVEPLAMFLRSGDIVLMAGEARECFHGVARIFTDKENADIT 137
            IVSMSLGCKAIFLLGGKSR+  PLAMF+RSGD+VLMAGEARECFHGV RIFTD+ENA+I 
Sbjct: 270  IVSMSLGCKAIFLLGGKSRDDPPLAMFVRSGDVVLMAGEARECFHGVPRIFTDQENAEIA 329

Query: 136  SLEQQLSNKADLCYLEYIKSSRININI 56
             LE   S++ D+C+LEYI+SSRININI
Sbjct: 330  PLELMFSHEDDVCFLEYIRSSRININI 356


>emb|CAN72697.1| hypothetical protein VITISV_011566 [Vitis vinifera]
          Length = 366

 Score =  450 bits (1158), Expect = e-124
 Identities = 222/322 (68%), Positives = 260/322 (80%), Gaps = 1/322 (0%)
 Frame = -1

Query: 1018 RKKQAIPVDLTQVLDFNSTLQSFNSNGELPPGVSLAAHQFDRPVFCLDEHPGFYFIPEAL 839
            RKKQ   VDL++V+DF + L+SFN +GE+PPG       FDRPVFC++  PGFYFIP+AL
Sbjct: 41   RKKQLKQVDLSEVVDFKAILRSFNQSGEVPPGTFALQCDFDRPVFCIENRPGFYFIPDAL 100

Query: 838  SVTEQCHWIKESLTTFPQPPNRTNHNAAYGPIHDLFIAAIQNKVLVENQNLSEGLLPDDK 659
            +V EQ  WI+ESL +FPQP NRTNHNA YGPI DLF+AA + K+LVE ++ S GL     
Sbjct: 101  TVEEQGRWIRESLISFPQPYNRTNHNAIYGPIRDLFMAAKERKILVEEEDSSGGLDSLSN 160

Query: 658  TRATEL-AYTWNSSEESIASLRESTCKSVSASTLLRKLRWTTLGLQFDWSQRSYNVGLPH 482
            +  T + A  W   EE+  S R STCKS+SAS LLRKLRW+TLGLQFDWS+R+YNV LPH
Sbjct: 161  SSITNMDAERWKFYEENTVSSRGSTCKSISASVLLRKLRWSTLGLQFDWSKRNYNVSLPH 220

Query: 481  NKIPDRLCQLAKKMAAPAMSLGEEFQPEAAIVNYFREGDMLGGHLDDMEADWSKPIVSMS 302
            NKIPD LC LAK+MAAPAMS G+ FQPEAAIVNYF  GDMLGGHLDDMEADW+KPIVSMS
Sbjct: 221  NKIPDALCDLAKEMAAPAMSKGKVFQPEAAIVNYFGLGDMLGGHLDDMEADWTKPIVSMS 280

Query: 301  LGCKAIFLLGGKSREVEPLAMFLRSGDIVLMAGEARECFHGVARIFTDKENADITSLEQQ 122
            LGCKAIFLLGGKSR+  PLAMF+RSGD+VLMAGEARECFHGV RIFTD+ENA+I  LE  
Sbjct: 281  LGCKAIFLLGGKSRDDPPLAMFVRSGDVVLMAGEARECFHGVPRIFTDQENAEIAPLELM 340

Query: 121  LSNKADLCYLEYIKSSRININI 56
             S++ D+C+LEYI+SSRININI
Sbjct: 341  FSHEDDVCFLEYIRSSRININI 362


>ref|XP_002304683.1| predicted protein [Populus trichocarpa] gi|222842115|gb|EEE79662.1|
            predicted protein [Populus trichocarpa]
          Length = 353

 Score =  444 bits (1141), Expect = e-122
 Identities = 225/321 (70%), Positives = 256/321 (79%)
 Frame = -1

Query: 1018 RKKQAIPVDLTQVLDFNSTLQSFNSNGELPPGVSLAAHQFDRPVFCLDEHPGFYFIPEAL 839
            +KKQ   VDL++VLDF S L S++ NGELPP + +   +FDRPVF L+  PGFYFIP AL
Sbjct: 43   KKKQPKQVDLSEVLDFKSFLDSYHQNGELPPEIVVVDCRFDRPVFGLESRPGFYFIPGAL 102

Query: 838  SVTEQCHWIKESLTTFPQPPNRTNHNAAYGPIHDLFIAAIQNKVLVENQNLSEGLLPDDK 659
            SV EQC WI+ESL +FPQPPNRTNHNA YGPI DLFIAA + KVLVE++N+     P + 
Sbjct: 103  SVDEQCRWIRESLMSFPQPPNRTNHNAIYGPISDLFIAAKERKVLVEDENM-----PANT 157

Query: 658  TRATELAYTWNSSEESIASLRESTCKSVSASTLLRKLRWTTLGLQFDWSQRSYNVGLPHN 479
             R       W+  EE    LR  +CK VSAS LLRKLRW+TLGLQFDWS+R+YNV LPHN
Sbjct: 158  RR-------WSFCEEDSVLLRGKSCKPVSASVLLRKLRWSTLGLQFDWSKRNYNVSLPHN 210

Query: 478  KIPDRLCQLAKKMAAPAMSLGEEFQPEAAIVNYFREGDMLGGHLDDMEADWSKPIVSMSL 299
            KIPD LCQLAKK+AAPAM +GEEF PEAAIVNYF  GD LGGHLDDMEADWSKPIVSMSL
Sbjct: 211  KIPDGLCQLAKKLAAPAMPVGEEFHPEAAIVNYFASGDTLGGHLDDMEADWSKPIVSMSL 270

Query: 298  GCKAIFLLGGKSREVEPLAMFLRSGDIVLMAGEARECFHGVARIFTDKENADITSLEQQL 119
            GCKAIFLLGGKSRE  PLAMFLRSGD+VLMAGEARECFHGV RIFTDKENA+IT+LE   
Sbjct: 271  GCKAIFLLGGKSREDPPLAMFLRSGDVVLMAGEARECFHGVPRIFTDKENAEITALELHF 330

Query: 118  SNKADLCYLEYIKSSRININI 56
             ++ D+  LEYI++SRININI
Sbjct: 331  CDENDI--LEYIRTSRININI 349


>gb|AEL99108.1| alpha-ketoglutarate-dependent dioxygenase alkB, partial [Silene
            latifolia]
          Length = 354

 Score =  429 bits (1102), Expect = e-118
 Identities = 216/327 (66%), Positives = 254/327 (77%), Gaps = 3/327 (0%)
 Frame = -1

Query: 1027 STGRKKQAIPVDLTQVLDFNSTLQSFNSNGELPPGVSLAAHQFDRPVFCLDEHPGFYFIP 848
            S  +K+Q  PVDL++V+DF   L++F+  GE P GV +     + PV CLD  PGFYFIP
Sbjct: 30   SRKKKQQPRPVDLSEVVDFKLILETFDRTGETPDGVHVIRTGSEFPVICLDNRPGFYFIP 89

Query: 847  EALSVTEQCHWIKESLTTFPQPPNRTNHNAAYGPIHDLFIAAIQNKVLVENQ---NLSEG 677
             ALS+ EQC WI+ESLT+FPQPPNRTNHNA YGPI+DLF AA + KVLVE +   N+++ 
Sbjct: 90   GALSLEEQCQWIRESLTSFPQPPNRTNHNALYGPINDLFAAAQEGKVLVEEELSPNVTQS 149

Query: 676  LLPDDKTRATELAYTWNSSEESIASLRESTCKSVSASTLLRKLRWTTLGLQFDWSQRSYN 497
            L   D  +A    +   SSE    SL+ S   S+ AS LLRKL W+TLGLQFDWSQR+YN
Sbjct: 150  L---DNGQARRWTF---SSESGNVSLKGSGGNSILASVLLRKLHWSTLGLQFDWSQRNYN 203

Query: 496  VGLPHNKIPDRLCQLAKKMAAPAMSLGEEFQPEAAIVNYFREGDMLGGHLDDMEADWSKP 317
            V LPHNKIPD+LCQLAKK+A PAM LG+ F+PEAAIVNYF  GD LGGHLDDME DWSKP
Sbjct: 204  VDLPHNKIPDKLCQLAKKLAVPAMPLGDNFKPEAAIVNYFASGDTLGGHLDDMELDWSKP 263

Query: 316  IVSMSLGCKAIFLLGGKSREVEPLAMFLRSGDIVLMAGEARECFHGVARIFTDKENADIT 137
            IVSMSLGCK IFLLGGKSRE EPLAMFLRSGD+VLMAGEARECFHGV RIFTD  N++IT
Sbjct: 264  IVSMSLGCKGIFLLGGKSREDEPLAMFLRSGDVVLMAGEARECFHGVPRIFTDAHNSEIT 323

Query: 136  SLEQQLSNKADLCYLEYIKSSRININI 56
            +LE+ LS  +D C+L+YIK+SRININI
Sbjct: 324  ALEKHLSTDSDHCFLDYIKTSRININI 350


>ref|XP_004135327.1| PREDICTED: alpha-ketoglutarate-dependent dioxygenase alkB-like
            [Cucumis sativus] gi|449529170|ref|XP_004171574.1|
            PREDICTED: alpha-ketoglutarate-dependent dioxygenase
            alkB-like [Cucumis sativus]
          Length = 363

 Score =  427 bits (1097), Expect = e-117
 Identities = 215/327 (65%), Positives = 251/327 (76%), Gaps = 3/327 (0%)
 Frame = -1

Query: 1027 STGRKKQAIPVDLTQVLDFNSTLQSFNSNGELPPGVSLAAHQFDRPVFCLDEHPGFYFIP 848
            S+ +KK    VDL++V+DF + L+S+  +G LP GV+      D PVFCL+  PGFYFIP
Sbjct: 33   SSKKKKLPKHVDLSEVIDFKNILESYQQDGSLPVGVNATTCDLDGPVFCLENRPGFYFIP 92

Query: 847  EALSVTEQCHWIKESLTTFPQPPNRTNHNAAYGPIHDLFIAAIQNKVLVENQNLSEGLLP 668
             ALS+ EQC WI+ESL  FPQPPNRTNHNA YGPI DLFIAA +NKVLVE+  +S+  L 
Sbjct: 93   GALSLQEQCQWIRESLMEFPQPPNRTNHNAIYGPIQDLFIAAKENKVLVEHDEISDFKLD 152

Query: 667  DDKTRATELAYT--WNSSEESIASLRESTC-KSVSASTLLRKLRWTTLGLQFDWSQRSYN 497
             D   +     T  W   EE+  S R  T  KS+ AS LLRKLRW+TLGLQFDWS+RSYN
Sbjct: 153  SDVEPSISNGNTHNWKFVEENTVSSRRGTAYKSIPASVLLRKLRWSTLGLQFDWSKRSYN 212

Query: 496  VGLPHNKIPDRLCQLAKKMAAPAMSLGEEFQPEAAIVNYFREGDMLGGHLDDMEADWSKP 317
            + LPHNKIP  LCQLAK+MAA AM  GEEF+PEAAIVNYF  GD LGGHLDDMEADWSKP
Sbjct: 213  ISLPHNKIPSALCQLAKRMAAAAMPTGEEFKPEAAIVNYFASGDTLGGHLDDMEADWSKP 272

Query: 316  IVSMSLGCKAIFLLGGKSREVEPLAMFLRSGDIVLMAGEARECFHGVARIFTDKENADIT 137
            IVSMSLGCKAIFLLGGKSR+  P+AMFLRSGD+VLMAGEARECFHGV RIF D+E+ +I+
Sbjct: 273  IVSMSLGCKAIFLLGGKSRQDPPIAMFLRSGDVVLMAGEARECFHGVPRIFIDEESEEIS 332

Query: 136  SLEQQLSNKADLCYLEYIKSSRININI 56
             LE  L+N+ DL  LEYI++SRININI
Sbjct: 333  FLETHLTNQDDLHCLEYIRTSRININI 359