BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00012749
         (1818 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002279531.1| PREDICTED: cytochrome P450 86B1 [Vitis vinif...   525   e-146
ref|XP_002279670.1| PREDICTED: cytochrome P450 86B1 [Vitis vinif...   518   e-144
ref|XP_002275905.1| PREDICTED: cytochrome P450 86B1 [Vitis vinif...   511   e-142
emb|CAN80536.1| hypothetical protein VITISV_035975 [Vitis vinifera]   493   e-137
ref|XP_004160508.1| PREDICTED: LOW QUALITY PROTEIN: cytochrome P...   488   e-135

>ref|XP_002279531.1| PREDICTED: cytochrome P450 86B1 [Vitis vinifera]
          Length = 507

 Score =  525 bits (1352), Expect = e-146
 Identities = 248/507 (48%), Positives = 346/507 (68%), Gaps = 9/507 (1%)
 Frame = +2

Query: 77   LILLGYFGVAMAVLSFYFVRNLRYENGISWNWPIVGMLPYLIANMHHAHDKSAECLNRLG 256
            + L+ Y  + +AV+ F F+     + G+ WNWPIVGM   L+ ++H  HD++AE L + G
Sbjct: 1    MALVSYPEILVAVIFFLFIGGFCSKKGVPWNWPIVGMFAGLLFHVHRIHDEAAEILEKTG 60

Query: 257  ATLFIKGPWFANMDMLLTVDPANVRHVLIDSCENFPKGGDLKHIFEEVFGAGLVNSEAEL 436
             T   KGPWF NMDML T DPANV +++  +  NFPKG + + +FE V G G+ N++ E 
Sbjct: 61   CTFLFKGPWFCNMDMLGTTDPANVHYIMSSNFSNFPKGTEFRKMFE-VLGDGIFNADGES 119

Query: 437  WKNQRKLAQNFFHYHQFLGLVVNASEEKINNGLLALIDNVVKKNQVVDMQDVFQRLSFDI 616
            W+NQRKL Q+  +  +F   +   +  K+  GL+ L+D++ ++ +VVD+QDVFQRL+FD 
Sbjct: 120  WRNQRKLGQSMINNPRFHRFLAKVTYNKVEKGLVPLLDHLSEQGRVVDLQDVFQRLTFDT 179

Query: 617  TCIFVTGFDPLCLSLDLPDVPFSKAMEEAGEVIAERHFMPMVLWKLLRLLNMGSEKKLCR 796
            TC+ VTGFD  CLS++ P+VPF+ A+E+  E +  RH +P  LW+LLR L +G EKKL +
Sbjct: 180  TCMLVTGFDTKCLSIEFPEVPFATALEDVEEAVFYRHALPETLWRLLRWLGIGKEKKLRK 239

Query: 797  AKKIIDHVVYTYISEKRKALSKA---------EPDLLTLHLSDKDNSLELDKNEKLVRDI 949
              K +DH +  YIS KR+ LSK            DLLT ++++ DN++ L  + K +RD 
Sbjct: 240  GWKTLDHTIAEYISMKREELSKGITKLREDEDGADLLTSYMTE-DNAIGLKCDGKFLRDT 298

Query: 950  VINFMFAGRDATSSALTWFLWLVITHPQVLAKIRGELIAIMPPQSENTKRHIFSSEELSH 1129
            ++NFM AGRD TSSALTWF WLV  +P V +KIR E+   +P + +   RHIF +E L+ 
Sbjct: 299  ILNFMIAGRDTTSSALTWFFWLVSKNPLVESKIREEIETAIPEKEDRKGRHIFKTEGLNK 358

Query: 1130 AYYLHAALSEAIRLYPPFPFQHKEAIKSDILPSGHHVHPKLRVIVSLYAMGRMVDVWGKD 1309
              YLHAA+ EA+RLYPP PFQHK  I+ D+LPSGH V PK+++   +YAMGRM  +WGKD
Sbjct: 359  LVYLHAAMCEALRLYPPVPFQHKAPIQPDVLPSGHKVDPKMKIFFPVYAMGRMTAIWGKD 418

Query: 1310 CHEFKPERWILKDGSIKHVPAYKFPTFGAGPHACLGKKVAYTQMKEVAAALLHNYDIQLV 1489
            C EFKPERWI + G IKH P+YKF +F AGP  C+GK+VA+TQMK VAA ++HNY +++V
Sbjct: 419  CLEFKPERWISELGKIKHEPSYKFFSFNAGPRTCIGKEVAFTQMKAVAATIIHNYHVEMV 478

Query: 1490 DGHTATPNLSMVLCMQEGLKVRVTNRW 1570
             GH   PN+S++L M+ G KVRV+ RW
Sbjct: 479  KGHHVAPNVSIILYMKRGFKVRVSKRW 505


>ref|XP_002279670.1| PREDICTED: cytochrome P450 86B1 [Vitis vinifera]
          Length = 502

 Score =  518 bits (1334), Expect = e-144
 Identities = 252/504 (50%), Positives = 345/504 (68%), Gaps = 9/504 (1%)
 Frame = +2

Query: 86   LGYFGVAMAVLSFYFVRNLRYENGISWNWPIVGMLPYLIANMHHAHDKSAECLNRLGATL 265
            +GY  + +AV+ F  +  L   N + WNWP+VGMLP++  N+    D+  E L +   T 
Sbjct: 4    IGYPEILLAVICFLLLHRLSNSNMLPWNWPLVGMLPWIFRNIQQIQDRCTEVLEQSSGTF 63

Query: 266  FIKGPWFANMDMLLTVDPANVRHVLIDSCENFPKGGDLKHIFEEVFGAGLVNSEAELWKN 445
             +KGPWFANMDML+T DPANV +++  +  NFPKG +   IF+ V G G+ NS+++LW+ 
Sbjct: 64   LLKGPWFANMDMLITSDPANVHYIMSTNFSNFPKGPEFLKIFD-VLGEGIFNSDSDLWRK 122

Query: 446  QRKLAQNFFHYHQFLGLVVNASEEKINNGLLALIDNVVKKNQVVDMQDVFQRLSFDITCI 625
            QRKLAQ   +  QF   +V  +E K++ GL+ ++++V K+  +VD+QD+FQR +FD TC 
Sbjct: 123  QRKLAQVLINDRQFQQFLVKTTENKVDEGLIPVLEHVSKQGLLVDLQDLFQRFTFDTTCK 182

Query: 626  FVTGFDPLCLSLDLPDVPFSKAMEEAGEVIAERHFMPMVLWKLLRLLNMGSEKKLCRAKK 805
             +TGFD  CLS++ P V F+KAM++  E I  RH +P  LWKL R L +G EKKL +  +
Sbjct: 183  LITGFDTKCLSIEFPRVLFAKAMDDVEEAIFFRHVVPESLWKLQRWLGIGEEKKLSKGWE 242

Query: 806  IIDHVVYTYISEKRKALSKA---------EPDLLTLHLSDKDNSLELDKNEKLVRDIVIN 958
             ID+V+  YIS K + LSK            DLLT ++++ D+++EL K+ K +RD ++N
Sbjct: 243  TIDNVIAEYISMKHEELSKGIAKLQEDEEGTDLLTSYMNE-DSTMEL-KSNKFLRDTIVN 300

Query: 959  FMFAGRDATSSALTWFLWLVITHPQVLAKIRGELIAIMPPQSENTKRHIFSSEELSHAYY 1138
            F+ AGRD TS+ALTWF WLV  +P V AKIR EL   +P + +  K HIF +EEL+   Y
Sbjct: 301  FLLAGRDTTSAALTWFFWLVSKNPLVEAKIREELKETIPEKDQ--KWHIFRTEELNKLVY 358

Query: 1139 LHAALSEAIRLYPPFPFQHKEAIKSDILPSGHHVHPKLRVIVSLYAMGRMVDVWGKDCHE 1318
            LH AL E +RLYPP PFQHK   ++D+LPSGH VHPK++++ SLYAMGRM  +WGKDC E
Sbjct: 359  LHGALCETLRLYPPVPFQHKAPHQADVLPSGHRVHPKMKILFSLYAMGRMTTIWGKDCFE 418

Query: 1319 FKPERWILKDGSIKHVPAYKFPTFGAGPHACLGKKVAYTQMKEVAAALLHNYDIQLVDGH 1498
            FKPERWI + G IK+ P+YKF  F AGP  CLGK VA+TQMK VAAA++HNY++QLV GH
Sbjct: 419  FKPERWITEQGGIKYEPSYKFFAFNAGPRTCLGKGVAFTQMKAVAAAIIHNYNVQLVQGH 478

Query: 1499 TATPNLSMVLCMQEGLKVRVTNRW 1570
               PN+S++L M+  L VRVT RW
Sbjct: 479  PVAPNVSIILHMKHELMVRVTKRW 502


>ref|XP_002275905.1| PREDICTED: cytochrome P450 86B1 [Vitis vinifera]
          Length = 507

 Score =  511 bits (1315), Expect = e-142
 Identities = 248/511 (48%), Positives = 344/511 (67%), Gaps = 13/511 (2%)
 Frame = +2

Query: 77   LILLGYFGVAMAVLSFYFVRNLRYENGISWNWPIVGMLPYLIANMHHAHDKSAECLNRLG 256
            + LLGY  + +AV+ F  +      N + WNWP++GM P +  ++H  HD+  + L + G
Sbjct: 1    MALLGYPEILLAVIFFIILGRFWNINDLPWNWPLLGMFPSVFLHLHRIHDRVTDILEKSG 60

Query: 257  ATLFIKGPWFANMDMLLTVDPANVRHVLIDSCENFPKGGDLKHIFEEVFGAGLVNSEAEL 436
             T  +KGPWF NMD+L+T DPANV +++     NFPKG +   IF+   G G+ NS+AE 
Sbjct: 61   CTFLVKGPWFCNMDLLVTTDPANVHYIMSSHFSNFPKGSEFSKIFD-FLGDGIFNSDAES 119

Query: 437  WKNQRKLAQ---NFFHYHQFLGLVVNASEEKINNGLLALIDNVVKK-NQVVDMQDVFQRL 604
            W+ QRKL Q   N    ++FLG +   ++ K+  GLL ++D + ++ ++ +D+QDVFQRL
Sbjct: 120  WRKQRKLGQLMINNVRLYRFLGKI---TQNKVEKGLLPILDYLSEQEDRAMDLQDVFQRL 176

Query: 605  SFDITCIFVTGFDPLCLSLDLPDVPFSKAMEEAGEVIAERHFMPMVLWKLLRLLNMGSEK 784
            +FD TC+ VTGFD  CLS++LP+VPF+ A+E+A E I  RH +P  LW+L R L +G EK
Sbjct: 177  TFDTTCMLVTGFDSKCLSIELPEVPFATALEDAEEAILHRHVLPETLWRLQRWLGIGKEK 236

Query: 785  KLCRAKKIIDHVVYTYISEKRKALSKA---------EPDLLTLHLSDKDNSLELDKNEKL 937
            K  +A + +DH +  YIS+KR  LSK            DLLT ++S+ D ++ L  + K 
Sbjct: 237  KHQKAWETLDHTIAEYISKKRDELSKGIINLQEEEDGVDLLTSYMSE-DITMGLKCDHKF 295

Query: 938  VRDIVINFMFAGRDATSSALTWFLWLVITHPQVLAKIRGELIAIMPPQSENTKRHIFSSE 1117
            +RD ++NFM AGRD TSSALTWF WLV  +P V +KIR EL + +P + +   RH F +E
Sbjct: 296  LRDTILNFMIAGRDTTSSALTWFFWLVSKNPLVESKIREELESAIPEKEDKKGRHTFKTE 355

Query: 1118 ELSHAYYLHAALSEAIRLYPPFPFQHKEAIKSDILPSGHHVHPKLRVIVSLYAMGRMVDV 1297
             L+   YLHAAL E +RLYPP PFQHK  ++ D+LPSGH V PK++++ SLYAMGRM  +
Sbjct: 356  ALNKLVYLHAALCETLRLYPPVPFQHKAPLQPDMLPSGHRVDPKMKILFSLYAMGRMTSI 415

Query: 1298 WGKDCHEFKPERWILKDGSIKHVPAYKFPTFGAGPHACLGKKVAYTQMKEVAAALLHNYD 1477
            WG DC EFKPERWI + G IKH P+YKF  F AGP  CLGK+VA+TQMK VAA ++HNY 
Sbjct: 416  WGTDCLEFKPERWISERGRIKHEPSYKFFAFNAGPRTCLGKEVAFTQMKAVAATIIHNYH 475

Query: 1478 IQLVDGHTATPNLSMVLCMQEGLKVRVTNRW 1570
            +Q+V+GH   PN+S++L M+ GL VRV+ RW
Sbjct: 476  VQVVEGHHVAPNVSIILYMKHGLMVRVSKRW 506


>emb|CAN80536.1| hypothetical protein VITISV_035975 [Vitis vinifera]
          Length = 471

 Score =  493 bits (1269), Expect = e-137
 Identities = 234/471 (49%), Positives = 324/471 (68%), Gaps = 9/471 (1%)
 Frame = +2

Query: 185  MLPYLIANMHHAHDKSAECLNRLGATLFIKGPWFANMDMLLTVDPANVRHVLIDSCENFP 364
            M   L+ ++H  HD++AE L + G T   KGPWF NMDML T DPANV +++  +  NFP
Sbjct: 1    MFAGLLFHVHRIHDEAAEILEKTGCTFLFKGPWFCNMDMLGTTDPANVHYIMSSNFSNFP 60

Query: 365  KGGDLKHIFEEVFGAGLVNSEAELWKNQRKLAQNFFHYHQFLGLVVNASEEKINNGLLAL 544
            KG + + +FE V G G+ N++ E W+NQRKL Q+  +  +F   +   +  K+  GL+ L
Sbjct: 61   KGTEFRKMFE-VLGDGIFNADGESWRNQRKLGQSMINNPRFHRFLAKVTYNKVEKGLVPL 119

Query: 545  IDNVVKKNQVVDMQDVFQRLSFDITCIFVTGFDPLCLSLDLPDVPFSKAMEEAGEVIAER 724
            +D++ ++ +VVD+QDVFQRL+FD TC+ VTGFD  CLS++ P+VPF+ A+E+  E +  R
Sbjct: 120  LDHLSEQGRVVDLQDVFQRLTFDTTCMLVTGFDTKCLSIEFPEVPFATALEDVEEAVFYR 179

Query: 725  HFMPMVLWKLLRLLNMGSEKKLCRAKKIIDHVVYTYISEKRKALSKA---------EPDL 877
            H +P  LW+LLR L +G EKKL +  K +DH +  YIS KR+ LSK            DL
Sbjct: 180  HALPETLWRLLRWLGIGKEKKLRKGWKTLDHTIAEYISMKREELSKGITKLREDEDGADL 239

Query: 878  LTLHLSDKDNSLELDKNEKLVRDIVINFMFAGRDATSSALTWFLWLVITHPQVLAKIRGE 1057
            LT ++++ DN++ L  + K +RD ++NFM AGRD TSSALTWF WLV  +P V +KIR E
Sbjct: 240  LTSYMTE-DNAIGLKCDGKFLRDTILNFMIAGRDTTSSALTWFFWLVSKNPLVESKIREE 298

Query: 1058 LIAIMPPQSENTKRHIFSSEELSHAYYLHAALSEAIRLYPPFPFQHKEAIKSDILPSGHH 1237
            +   +P + +   RHIF +E L+   YLHAA+ EA+RLYPP PFQHK  I+ D+LPSGH 
Sbjct: 299  IETAIPEKEDRKGRHIFKTEGLNKLVYLHAAMCEALRLYPPVPFQHKAPIQPDVLPSGHK 358

Query: 1238 VHPKLRVIVSLYAMGRMVDVWGKDCHEFKPERWILKDGSIKHVPAYKFPTFGAGPHACLG 1417
            V PK+++   +YAMGRM  +WGKDC EFKPERWI + G IKH P+YKF +F AGP  C+G
Sbjct: 359  VDPKMKIFFPVYAMGRMTAIWGKDCLEFKPERWISELGKIKHEPSYKFFSFNAGPRTCIG 418

Query: 1418 KKVAYTQMKEVAAALLHNYDIQLVDGHTATPNLSMVLCMQEGLKVRVTNRW 1570
            K+VA+TQMK VAA ++HNY +++V GH   PN+S++L M+ G KVRV+ RW
Sbjct: 419  KEVAFTQMKAVAATIIHNYHVEMVKGHHVAPNVSIILYMKRGFKVRVSKRW 469


>ref|XP_004160508.1| PREDICTED: LOW QUALITY PROTEIN: cytochrome P450 86B1-like [Cucumis
            sativus]
          Length = 511

 Score =  488 bits (1256), Expect = e-135
 Identities = 238/484 (49%), Positives = 324/484 (66%), Gaps = 11/484 (2%)
 Frame = +2

Query: 158  ISWNWPIVGMLPYLIANMHHAHDKSAECLNRLGATLFIKGPWFANMDMLLTVDPANVRHV 337
            I WNWPIVGM P +IA++H  HD+  E L +   T F KG WF+ MD L TVDP+N+ H+
Sbjct: 35   IPWNWPIVGMTPTVIAHIHRPHDRVTEVLQQTAFTFFFKGIWFSGMDFLFTVDPSNIHHI 94

Query: 338  LIDSCENFPKGGDLKHIFEEVFGAGLVNSEAELWKNQRKLAQNFFHYHQFLGLVVNASEE 517
            L  + E +PKG D K+IFE V G G+ NS++++WKNQRK A     +H FL  +   +  
Sbjct: 95   LSANFERYPKGPDFKYIFE-VLGDGIFNSDSDVWKNQRKTAHYLVRHHNFLQFLEKITLN 153

Query: 518  KINNGLLALIDNVVKKNQVVDMQDVFQRLSFDITCIFVTGFDPLCLSLDLPDVPFSKAME 697
            K+  GL+ ++D+V +   V+D+QD+FQR SFD TC+ VTGFD   LSL+ P+VPFSKAM+
Sbjct: 154  KVKTGLVPILDSVCENGSVLDLQDLFQRFSFDSTCMMVTGFDLNSLSLEFPEVPFSKAMD 213

Query: 698  EAGEVIAERHFMPMVLWKLLRLLNMGSEKKLCRAKKIIDHVVYTYISEKRKALS------ 859
            +A EVI  RHF P  +W+  + L +G  K+L RA +IID  +   I+ KRK+L       
Sbjct: 214  DAEEVIFVRHFFPKTIWEFQKKLQIGQPKRLKRAWEIIDETIAKLIASKRKSLKNEMKEE 273

Query: 860  ----KAEPDLLTLHLSDKDNSLELDKNEKLVRDIVINFMFAGRDATSSALTWFLWLVITH 1027
                K   DL+T ++++ +N      +++ +RD V+NFM AGRD  SSAL+WF + +  H
Sbjct: 274  DDNGKEGVDLITSYITNIEN------DDEFLRDTVLNFMIAGRDTLSSALSWFFFXLSNH 327

Query: 1028 PQVLAKIRGELIAIMPPQSENTKRHIFSSEELSHAYYLHAALSEAIRLYPPFPFQHKEAI 1207
            P V+ KIR EL   +P      +R IFS EE+ +  Y H  L EA+RLYPP P QHK +I
Sbjct: 328  PTVVEKIREELRTTIPTNEAYDQRRIFSIEEVDNLVYFHGTLCEALRLYPPVPLQHKVSI 387

Query: 1208 KSDILPSGHHVHPKLRVIVSLYAMGRMVDVWGKDCHEFKPERWI-LKDGSIKHVPAYKFP 1384
            + DILPSGHH+ PK +++ SLYA+GRM +VWGKDC EFKPERWI  ++G IKHVP+YKF 
Sbjct: 388  QHDILPSGHHIKPKTKILFSLYALGRMSEVWGKDCLEFKPERWINSENGKIKHVPSYKFL 447

Query: 1385 TFGAGPHACLGKKVAYTQMKEVAAALLHNYDIQLVDGHTATPNLSMVLCMQEGLKVRVTN 1564
             F AGP  CLGK VA+T++K VAAA++HNY+I    GH   P+ S++L M+ G KV+VT 
Sbjct: 448  AFNAGPRTCLGKHVAFTELKIVAAAIIHNYNIIQQAGHEVVPSASIILHMKHGFKVKVTK 507

Query: 1565 RWSS 1576
            RWS+
Sbjct: 508  RWST 511