BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00012674
         (356 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ADO51748.1| alcohol dehydrogenase [Camellia sinensis]              199   1e-49
ref|XP_002279359.1| PREDICTED: NADP-dependent alkenal double bon...   197   6e-49
pdb|4HFJ|A Chain A, X-ray Crystal Structure Of A Double Bond Red...   195   3e-48
dbj|BAA89423.1| allyl alcohol dehydrogenase [Nicotiana tabacum]       195   3e-48
emb|CBI24733.3| unnamed protein product [Vitis vinifera]              188   3e-46

>gb|ADO51748.1| alcohol dehydrogenase [Camellia sinensis]
          Length = 347

 Score =  199 bits (507), Expect = 1e-49
 Identities = 93/118 (78%), Positives = 107/118 (90%)
 Frame = +2

Query: 2   VGSAGTNEKVELLKNKLGFDEAFNYKEEQDLTAALKRYFPSGIDIYFENVGGKMLDAVLL 181
           VGSAGT EKV+LLKNK GFDEAFNYKEE+DL AALKRYFP+GI+IYFENVGGKMLDAVL 
Sbjct: 186 VGSAGTKEKVDLLKNKFGFDEAFNYKEEKDLEAALKRYFPNGINIYFENVGGKMLDAVLA 245

Query: 182 NMNAHGCIAVCGMISQYNLDKPEGVQNLFCLISKQIRMEGFVASEYYNLYPKFLELVL 355
           NM  H  IAVCGMISQYNL++PEGV NLFCLI+K++RMEGF+  +YY+LYPKFLE++L
Sbjct: 246 NMRLHSRIAVCGMISQYNLERPEGVHNLFCLITKRVRMEGFIVFDYYHLYPKFLEMIL 303


>ref|XP_002279359.1| PREDICTED: NADP-dependent alkenal double bond reductase P2 [Vitis
           vinifera] gi|147792339|emb|CAN61471.1| hypothetical
           protein VITISV_043825 [Vitis vinifera]
          Length = 345

 Score =  197 bits (502), Expect = 6e-49
 Identities = 92/118 (77%), Positives = 107/118 (90%)
 Frame = +2

Query: 2   VGSAGTNEKVELLKNKLGFDEAFNYKEEQDLTAALKRYFPSGIDIYFENVGGKMLDAVLL 181
           VGSAGT EKV+LLKNK GFDEAFNYKEEQDL A LKRYFP GIDIYFENVGGKMLDAVL+
Sbjct: 184 VGSAGTKEKVDLLKNKFGFDEAFNYKEEQDLEACLKRYFPEGIDIYFENVGGKMLDAVLV 243

Query: 182 NMNAHGCIAVCGMISQYNLDKPEGVQNLFCLISKQIRMEGFVASEYYNLYPKFLELVL 355
           NM  HG IAVCGMISQYNL++PEGV+NLF +++K+IRMEGF+  +YY+LYPKFL+L++
Sbjct: 244 NMRLHGRIAVCGMISQYNLEEPEGVRNLFTIVTKRIRMEGFLVFDYYHLYPKFLDLIM 301


>pdb|4HFJ|A Chain A, X-ray Crystal Structure Of A Double Bond Reductase From
           Nicotiana Tabacum gi|444302247|pdb|4HFJ|B Chain B, X-ray
           Crystal Structure Of A Double Bond Reductase From
           Nicotiana Tabacum gi|444302248|pdb|4HFM|A Chain A, X-ray
           Crystal Structure Of A Nadp(h)-bound Double Bond
           Reductase From Nicotiana Tabacum gi|444302249|pdb|4HFM|B
           Chain B, X-ray Crystal Structure Of A Nadp(h)-bound
           Double Bond Reductase From Nicotiana Tabacum
           gi|444302250|pdb|4HFN|A Chain A, X-ray Crystal Structure
           Of A Ternary Complex Of Double Bond Reductase From
           Nicotiana Tabacum gi|444302251|pdb|4HFN|B Chain B, X-ray
           Crystal Structure Of A Ternary Complex Of Double Bond
           Reductase From Nicotiana Tabacum
          Length = 351

 Score =  195 bits (496), Expect = 3e-48
 Identities = 92/118 (77%), Positives = 108/118 (91%)
 Frame = +2

Query: 2   VGSAGTNEKVELLKNKLGFDEAFNYKEEQDLTAALKRYFPSGIDIYFENVGGKMLDAVLL 181
           VGSAG+ EKV+LLK+K GFDEAFNYKEEQDL+AALKRYFP GIDIYFENVGGKMLDAVL+
Sbjct: 182 VGSAGSKEKVDLLKSKFGFDEAFNYKEEQDLSAALKRYFPDGIDIYFENVGGKMLDAVLV 241

Query: 182 NMNAHGCIAVCGMISQYNLDKPEGVQNLFCLISKQIRMEGFVASEYYNLYPKFLELVL 355
           NM  +G IAVCGMISQYNL++ EGV NLFCLI+K+IRMEGF+  +YY+LYPK+LE+V+
Sbjct: 242 NMKLYGRIAVCGMISQYNLEQTEGVHNLFCLITKRIRMEGFLVFDYYHLYPKYLEMVI 299


>dbj|BAA89423.1| allyl alcohol dehydrogenase [Nicotiana tabacum]
          Length = 343

 Score =  195 bits (496), Expect = 3e-48
 Identities = 92/118 (77%), Positives = 108/118 (91%)
 Frame = +2

Query: 2   VGSAGTNEKVELLKNKLGFDEAFNYKEEQDLTAALKRYFPSGIDIYFENVGGKMLDAVLL 181
           VGSAG+ EKV+LLK+K GFDEAFNYKEEQDL+AALKRYFP GIDIYFENVGGKMLDAVL+
Sbjct: 182 VGSAGSKEKVDLLKSKFGFDEAFNYKEEQDLSAALKRYFPDGIDIYFENVGGKMLDAVLV 241

Query: 182 NMNAHGCIAVCGMISQYNLDKPEGVQNLFCLISKQIRMEGFVASEYYNLYPKFLELVL 355
           NM  +G IAVCGMISQYNL++ EGV NLFCLI+K+IRMEGF+  +YY+LYPK+LE+V+
Sbjct: 242 NMKLYGRIAVCGMISQYNLEQTEGVHNLFCLITKRIRMEGFLVFDYYHLYPKYLEMVI 299


>emb|CBI24733.3| unnamed protein product [Vitis vinifera]
          Length = 365

 Score =  188 bits (478), Expect = 3e-46
 Identities = 89/118 (75%), Positives = 99/118 (83%)
 Frame = +2

Query: 2   VGSAGTNEKVELLKNKLGFDEAFNYKEEQDLTAALKRYFPSGIDIYFENVGGKMLDAVLL 181
           VGSAGT EKV+LLKNK GFDEAFNYKEEQDL   LKRYFP GIDIYFENVGG MLDAVL 
Sbjct: 204 VGSAGTKEKVDLLKNKFGFDEAFNYKEEQDLATCLKRYFPEGIDIYFENVGGAMLDAVLP 263

Query: 182 NMNAHGCIAVCGMISQYNLDKPEGVQNLFCLISKQIRMEGFVASEYYNLYPKFLELVL 355
           NM  HG IA CGMISQYNLDKPEG+ NL  +I+KQI+M+GF+   Y++LYPKFLE +L
Sbjct: 264 NMRVHGRIAACGMISQYNLDKPEGIYNLMNVINKQIKMQGFITPSYFHLYPKFLETIL 321