BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00012582
         (1571 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI23872.3| unnamed protein product [Vitis vinifera]              671   0.0  
ref|XP_002276384.1| PREDICTED: 3-isopropylmalate dehydrogenase, ...   670   0.0  
ref|XP_002530452.1| 3-isopropylmalate dehydrogenase, putative [R...   660   0.0  
gb|ACF17658.1| putative 3-isopropylmalate dehydrogenase large su...   653   0.0  
ref|XP_002298230.1| predicted protein [Populus trichocarpa] gi|2...   647   0.0  

>emb|CBI23872.3| unnamed protein product [Vitis vinifera]
          Length = 506

 Score =  671 bits (1732), Expect = 0.0
 Identities = 343/408 (84%), Positives = 369/408 (90%)
 Frame = -3

Query: 1497 TKMAASLQVSLRPFKPXXXXXXXXXXXHPKKFATIRCSAATPSKRYTITLLPGDGIGPEV 1318
            T MAASLQ+S RPFKP             +  ATIRCSAATP KRY+ITLLPGDGIGPEV
Sbjct: 100  TPMAASLQLSARPFKPHFQSKSLSKPSSIR-LATIRCSAATPPKRYSITLLPGDGIGPEV 158

Query: 1317 ISVARNVLNLVGSIEGIEFGFKEMPMGGAALDLTGVPLPEETLSEAKNSDAVLLGAIGGY 1138
            ISVA+NVL L GS+EGIEFGF+EMP+GG ALDLTGVPLPEETL  AK SDAVLLGAIGGY
Sbjct: 159  ISVAKNVLKLAGSLEGIEFGFQEMPVGGCALDLTGVPLPEETLLVAKQSDAVLLGAIGGY 218

Query: 1137 KWDNNEKHLKPETGLLQLREGLKVFANLRPASVLPQLVDASTLKKEVAEGVDLMVVRELT 958
            KWD+NEKHLKPETGLLQLR GLKVFANLRPASVLPQLVDASTLKKEVAEGVDLMVVRELT
Sbjct: 219  KWDSNEKHLKPETGLLQLRSGLKVFANLRPASVLPQLVDASTLKKEVAEGVDLMVVRELT 278

Query: 957  GGIYFGKPRGFSTNDSGDEIGFNTEVYAAYEIDRIAHVAFETARKRRCKLCSVDKANVLE 778
            GGIYFGKPRGFS N++G+EIGFNTEVYA YEIDRIA +AFETA+KRR KLCSVDKANVLE
Sbjct: 279  GGIYFGKPRGFSKNENGEEIGFNTEVYATYEIDRIARIAFETAQKRRGKLCSVDKANVLE 338

Query: 777  ASMLWRKRVTAIASQYPDVELSHMYVDNAAMQLVRNPKQFDTIVTNNIFGDILSDEASMI 598
            ASMLWRKRVTA+AS+YPDVELSHMYVDNAAMQLVRNPKQFDTIVTNNIFGDILSDEASMI
Sbjct: 339  ASMLWRKRVTAMASEYPDVELSHMYVDNAAMQLVRNPKQFDTIVTNNIFGDILSDEASMI 398

Query: 597  TGSIGMLPSASIGDSGPGLYEPIHGSAPDIAGMDKANPLATVLSAAMLLKYGLGEQKAAQ 418
            TGSIGMLPSAS+G+SGPGL+EPIHGSAPDIAG DKANPLATVLSAAMLL+YGLGE  AA+
Sbjct: 399  TGSIGMLPSASVGESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLRYGLGEGNAAK 458

Query: 417  RIELAVLDTLNRGFRTGDIYSAGNKLVGCTEMGEEVLKSVDSKLPAVV 274
            RIE AVLDTL+RGFRT DIY AG KLVGC EMGE+VLK+V+S++ A V
Sbjct: 459  RIETAVLDTLDRGFRTRDIYEAGTKLVGCKEMGEQVLKAVESQIRAAV 506


>ref|XP_002276384.1| PREDICTED: 3-isopropylmalate dehydrogenase, chloroplastic [Vitis
            vinifera]
          Length = 405

 Score =  670 bits (1728), Expect = 0.0
 Identities = 342/406 (84%), Positives = 368/406 (90%)
 Frame = -3

Query: 1491 MAASLQVSLRPFKPXXXXXXXXXXXHPKKFATIRCSAATPSKRYTITLLPGDGIGPEVIS 1312
            MAASLQ+S RPFKP             +  ATIRCSAATP KRY+ITLLPGDGIGPEVIS
Sbjct: 1    MAASLQLSARPFKPHFQSKSLSKPSSIR-LATIRCSAATPPKRYSITLLPGDGIGPEVIS 59

Query: 1311 VARNVLNLVGSIEGIEFGFKEMPMGGAALDLTGVPLPEETLSEAKNSDAVLLGAIGGYKW 1132
            VA+NVL L GS+EGIEFGF+EMP+GG ALDLTGVPLPEETL  AK SDAVLLGAIGGYKW
Sbjct: 60   VAKNVLKLAGSLEGIEFGFQEMPVGGCALDLTGVPLPEETLLVAKQSDAVLLGAIGGYKW 119

Query: 1131 DNNEKHLKPETGLLQLREGLKVFANLRPASVLPQLVDASTLKKEVAEGVDLMVVRELTGG 952
            D+NEKHLKPETGLLQLR GLKVFANLRPASVLPQLVDASTLKKEVAEGVDLMVVRELTGG
Sbjct: 120  DSNEKHLKPETGLLQLRSGLKVFANLRPASVLPQLVDASTLKKEVAEGVDLMVVRELTGG 179

Query: 951  IYFGKPRGFSTNDSGDEIGFNTEVYAAYEIDRIAHVAFETARKRRCKLCSVDKANVLEAS 772
            IYFGKPRGFS N++G+EIGFNTEVYA YEIDRIA +AFETA+KRR KLCSVDKANVLEAS
Sbjct: 180  IYFGKPRGFSKNENGEEIGFNTEVYATYEIDRIARIAFETAQKRRGKLCSVDKANVLEAS 239

Query: 771  MLWRKRVTAIASQYPDVELSHMYVDNAAMQLVRNPKQFDTIVTNNIFGDILSDEASMITG 592
            MLWRKRVTA+AS+YPDVELSHMYVDNAAMQLVRNPKQFDTIVTNNIFGDILSDEASMITG
Sbjct: 240  MLWRKRVTAMASEYPDVELSHMYVDNAAMQLVRNPKQFDTIVTNNIFGDILSDEASMITG 299

Query: 591  SIGMLPSASIGDSGPGLYEPIHGSAPDIAGMDKANPLATVLSAAMLLKYGLGEQKAAQRI 412
            SIGMLPSAS+G+SGPGL+EPIHGSAPDIAG DKANPLATVLSAAMLL+YGLGE  AA+RI
Sbjct: 300  SIGMLPSASVGESGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLRYGLGEGNAAKRI 359

Query: 411  ELAVLDTLNRGFRTGDIYSAGNKLVGCTEMGEEVLKSVDSKLPAVV 274
            E AVLDTL+RGFRT DIY AG KLVGC EMGE+VLK+V+S++ A V
Sbjct: 360  ETAVLDTLDRGFRTRDIYEAGTKLVGCKEMGEQVLKAVESQIRAAV 405


>ref|XP_002530452.1| 3-isopropylmalate dehydrogenase, putative [Ricinus communis]
            gi|223529997|gb|EEF31922.1| 3-isopropylmalate
            dehydrogenase, putative [Ricinus communis]
          Length = 406

 Score =  660 bits (1704), Expect = 0.0
 Identities = 334/406 (82%), Positives = 362/406 (89%)
 Frame = -3

Query: 1491 MAASLQVSLRPFKPXXXXXXXXXXXHPKKFATIRCSAATPSKRYTITLLPGDGIGPEVIS 1312
            MAA+ Q +++P K                  TI+C+A TPSKRY+ITLLPGDGIGPEVIS
Sbjct: 1    MAAATQFNIKPNKAIFHSKLTSKHCLKPGRFTIKCAAVTPSKRYSITLLPGDGIGPEVIS 60

Query: 1311 VARNVLNLVGSIEGIEFGFKEMPMGGAALDLTGVPLPEETLSEAKNSDAVLLGAIGGYKW 1132
            +A+NVL L G++EGIEF F+EMPMGG+ALDL GVPLP+ETLS AK SDAVLLGAIGGYKW
Sbjct: 61   IAKNVLKLAGNLEGIEFSFQEMPMGGSALDLVGVPLPDETLSVAKQSDAVLLGAIGGYKW 120

Query: 1131 DNNEKHLKPETGLLQLREGLKVFANLRPASVLPQLVDASTLKKEVAEGVDLMVVRELTGG 952
            DNNEKHLKPETGLLQLREGL+VFANLRPA+VLPQLVDASTLKKEVAEGVD+MVVRELTGG
Sbjct: 121  DNNEKHLKPETGLLQLREGLEVFANLRPATVLPQLVDASTLKKEVAEGVDVMVVRELTGG 180

Query: 951  IYFGKPRGFSTNDSGDEIGFNTEVYAAYEIDRIAHVAFETARKRRCKLCSVDKANVLEAS 772
            IYFGKPRGF TN+SG+EIGFNTEVYA YE+DRIA VAFETARKR  KLCSVDKANVLEAS
Sbjct: 181  IYFGKPRGFGTNESGEEIGFNTEVYAGYEVDRIARVAFETARKRSGKLCSVDKANVLEAS 240

Query: 771  MLWRKRVTAIASQYPDVELSHMYVDNAAMQLVRNPKQFDTIVTNNIFGDILSDEASMITG 592
            M WRKRV AIA +YPDVELSHMYVDNAAMQLVRNPKQFDTIVTNNIFGDILSDEASMITG
Sbjct: 241  MFWRKRVIAIAEEYPDVELSHMYVDNAAMQLVRNPKQFDTIVTNNIFGDILSDEASMITG 300

Query: 591  SIGMLPSASIGDSGPGLYEPIHGSAPDIAGMDKANPLATVLSAAMLLKYGLGEQKAAQRI 412
            SIGMLPSAS+G SGPGL+EPIHGSAPDIAG DKANPLATVLSAAMLLKYGLGE KAA+RI
Sbjct: 301  SIGMLPSASLGSSGPGLFEPIHGSAPDIAGQDKANPLATVLSAAMLLKYGLGEDKAAKRI 360

Query: 411  ELAVLDTLNRGFRTGDIYSAGNKLVGCTEMGEEVLKSVDSKLPAVV 274
            E AVLDTL+ GFRTGDIYSAG KLVGC EMG+EVLKSVDS++PA V
Sbjct: 361  ENAVLDTLDMGFRTGDIYSAGTKLVGCKEMGDEVLKSVDSQVPAAV 406


>gb|ACF17658.1| putative 3-isopropylmalate dehydrogenase large subunit [Capsicum
            annuum]
          Length = 406

 Score =  653 bits (1684), Expect = 0.0
 Identities = 327/378 (86%), Positives = 351/378 (92%)
 Frame = -3

Query: 1407 KFATIRCSAATPSKRYTITLLPGDGIGPEVISVARNVLNLVGSIEGIEFGFKEMPMGGAA 1228
            K+ TIRCSA T SK Y ITLLPGDGIGPEVISVA+N L LV S+EG E  F+EMPMGGAA
Sbjct: 29   KWGTIRCSATTQSKSYNITLLPGDGIGPEVISVAKNALQLVASLEGFEVAFEEMPMGGAA 88

Query: 1227 LDLTGVPLPEETLSEAKNSDAVLLGAIGGYKWDNNEKHLKPETGLLQLREGLKVFANLRP 1048
            LD  GVPLP+ETL  AK SDA LLGAIGGYKWDNN KHLKPETGLLQLR+GL+VFANLRP
Sbjct: 89   LDAVGVPLPDETLKSAKQSDATLLGAIGGYKWDNNAKHLKPETGLLQLRKGLQVFANLRP 148

Query: 1047 ASVLPQLVDASTLKKEVAEGVDLMVVRELTGGIYFGKPRGFSTNDSGDEIGFNTEVYAAY 868
            A+VLPQLVDASTLKKEVAEGVDLMVVRELTGGIYFGKPRGFST+++G+EIGFNTEVYA Y
Sbjct: 149  ATVLPQLVDASTLKKEVAEGVDLMVVRELTGGIYFGKPRGFSTDENGEEIGFNTEVYATY 208

Query: 867  EIDRIAHVAFETARKRRCKLCSVDKANVLEASMLWRKRVTAIASQYPDVELSHMYVDNAA 688
            EIDRIA +AFETARKRR KLCSVDKANVLEASMLWRKRVTA+AS+YPDVELSHMYVDNAA
Sbjct: 209  EIDRIARIAFETARKRRGKLCSVDKANVLEASMLWRKRVTALASEYPDVELSHMYVDNAA 268

Query: 687  MQLVRNPKQFDTIVTNNIFGDILSDEASMITGSIGMLPSASIGDSGPGLYEPIHGSAPDI 508
            MQLVRNPKQFDTIVTNNIFGDILSDEASMITGSIGMLPSAS+G SGPGL+EPIHGSAPDI
Sbjct: 269  MQLVRNPKQFDTIVTNNIFGDILSDEASMITGSIGMLPSASLGASGPGLFEPIHGSAPDI 328

Query: 507  AGMDKANPLATVLSAAMLLKYGLGEQKAAQRIELAVLDTLNRGFRTGDIYSAGNKLVGCT 328
            AG DKANPLAT+LSAAMLLKYGLGE+KAA+RIE AVLD+LNRGFRTGDI+SAG+KLVGC 
Sbjct: 329  AGQDKANPLATMLSAAMLLKYGLGEEKAARRIEAAVLDSLNRGFRTGDIHSAGHKLVGCK 388

Query: 327  EMGEEVLKSVDSKLPAVV 274
            EMGEEVLKS+DSK PA V
Sbjct: 389  EMGEEVLKSIDSKTPAAV 406


>ref|XP_002298230.1| predicted protein [Populus trichocarpa] gi|222845488|gb|EEE83035.1|
            predicted protein [Populus trichocarpa]
          Length = 400

 Score =  647 bits (1668), Expect = 0.0
 Identities = 326/376 (86%), Positives = 350/376 (93%), Gaps = 2/376 (0%)
 Frame = -3

Query: 1395 IRCSAATP--SKRYTITLLPGDGIGPEVISVARNVLNLVGSIEGIEFGFKEMPMGGAALD 1222
            IRC+AAT   SKRY+ITLLPGDGIGPEVISVA+NVLNL GS+EGIEF F+EMPMGG+ALD
Sbjct: 25   IRCAAATTKQSKRYSITLLPGDGIGPEVISVAKNVLNLAGSLEGIEFNFQEMPMGGSALD 84

Query: 1221 LTGVPLPEETLSEAKNSDAVLLGAIGGYKWDNNEKHLKPETGLLQLREGLKVFANLRPAS 1042
            + GVPLP+ETL  AK SDAVLLGAIGGYKWD NEKHLKPETGLLQLREGL+VFANLRPA+
Sbjct: 85   MAGVPLPDETLLAAKKSDAVLLGAIGGYKWDKNEKHLKPETGLLQLREGLEVFANLRPAT 144

Query: 1041 VLPQLVDASTLKKEVAEGVDLMVVRELTGGIYFGKPRGFSTNDSGDEIGFNTEVYAAYEI 862
            VLPQLVDASTLKKEVAEGVD+MVVRELTGGIYFGKPRGF TN+ G+EIGFNTEVYA YE+
Sbjct: 145  VLPQLVDASTLKKEVAEGVDVMVVRELTGGIYFGKPRGFGTNEDGEEIGFNTEVYATYEV 204

Query: 861  DRIAHVAFETARKRRCKLCSVDKANVLEASMLWRKRVTAIASQYPDVELSHMYVDNAAMQ 682
            DRIA VAFETARKR  KLCSVDKANVLEASM WRKRV +IA++YPDVELSHMYVDNAAMQ
Sbjct: 205  DRIARVAFETARKRHGKLCSVDKANVLEASMFWRKRVISIAAEYPDVELSHMYVDNAAMQ 264

Query: 681  LVRNPKQFDTIVTNNIFGDILSDEASMITGSIGMLPSASIGDSGPGLYEPIHGSAPDIAG 502
            LVRNPKQFDTIVTNNIFGDILSDEASMITGSIGMLPSAS+G SGPGL+EPIHGSAPDIA 
Sbjct: 265  LVRNPKQFDTIVTNNIFGDILSDEASMITGSIGMLPSASLGSSGPGLFEPIHGSAPDIAN 324

Query: 501  MDKANPLATVLSAAMLLKYGLGEQKAAQRIELAVLDTLNRGFRTGDIYSAGNKLVGCTEM 322
             DKANPLATVLSAAMLLKYGLGE+ AA+RIE AVLDTLN+GFRTGDIYSAGNKLVGC EM
Sbjct: 325  QDKANPLATVLSAAMLLKYGLGEENAAKRIENAVLDTLNKGFRTGDIYSAGNKLVGCKEM 384

Query: 321  GEEVLKSVDSKLPAVV 274
            GEEVLKSVDS++ A V
Sbjct: 385  GEEVLKSVDSQVAAAV 400