BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00012477
         (677 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI30150.3| unnamed protein product [Vitis vinifera]              222   6e-56
ref|XP_002266033.1| PREDICTED: E3 ubiquitin-protein ligase RFWD3...   222   6e-56
emb|CAN68707.1| hypothetical protein VITISV_001643 [Vitis vinifera]   220   2e-55
ref|XP_002326378.1| predicted protein [Populus trichocarpa] gi|2...   200   2e-49
ref|XP_004143360.1| PREDICTED: E3 ubiquitin-protein ligase RFWD3...   190   3e-46

>emb|CBI30150.3| unnamed protein product [Vitis vinifera]
          Length = 610

 Score =  222 bits (565), Expect = 6e-56
 Identities = 111/221 (50%), Positives = 148/221 (66%)
 Frame = +1

Query: 1    CSWDFSCLNHAYAGLQNGMLLVFDMRQTRGPVESLVGLTCNPVHXXXXXXXXXXXXXXDK 180
            CSWD +  ++ YAGLQNGML+VFDMRQT  P+  + G T NP+H                
Sbjct: 369  CSWDLNSSHYMYAGLQNGMLMVFDMRQTARPMAEISGPTSNPIHTIQSVLHNSTLPSGVS 428

Query: 181  TVITASSVGICEWNFGGVDKRPFLVPGSENHGVCISLACSPRNNEIVASYRPKVKTTDEA 360
            T+++ASSVG+C+WNFGG ++RPFLVP ++NHGVCISLA  P ++ IV+++RPKV+ ++E 
Sbjct: 429  TLLSASSVGLCQWNFGGSEERPFLVPETKNHGVCISLAHCPSSDNIVSTFRPKVEMSNEI 488

Query: 361  IASQAFRSTATCPMGNFVLGTHVSYKRLSSQCYQQLGYTSANVNDIRLPKSAIVDICDDT 540
              SQ   S     MG  + G+HV  KR+   CYQ+LG + ANVNDIRL +SAI+D+    
Sbjct: 489  AVSQY--SLTPSVMGQGMQGSHVLLKRVGGNCYQKLGSSCANVNDIRLQRSAIIDLQSHH 546

Query: 541  ALFVSGDEVRCEVTMQALPSLVTVQNLKLPKFPFRDVKFTH 663
             LFVSGDE+  E+ +Q LPSL   Q LK  K P RDVK++H
Sbjct: 547  PLFVSGDEITHELVLQELPSLRVSQRLKSQKHPLRDVKYSH 587


>ref|XP_002266033.1| PREDICTED: E3 ubiquitin-protein ligase RFWD3-like [Vitis vinifera]
          Length = 567

 Score =  222 bits (565), Expect = 6e-56
 Identities = 111/221 (50%), Positives = 148/221 (66%)
 Frame = +1

Query: 1   CSWDFSCLNHAYAGLQNGMLLVFDMRQTRGPVESLVGLTCNPVHXXXXXXXXXXXXXXDK 180
           CSWD +  ++ YAGLQNGML+VFDMRQT  P+  + G T NP+H                
Sbjct: 326 CSWDLNSSHYMYAGLQNGMLMVFDMRQTARPMAEISGPTSNPIHTIQSVLHNSTLPSGVS 385

Query: 181 TVITASSVGICEWNFGGVDKRPFLVPGSENHGVCISLACSPRNNEIVASYRPKVKTTDEA 360
           T+++ASSVG+C+WNFGG ++RPFLVP ++NHGVCISLA  P ++ IV+++RPKV+ ++E 
Sbjct: 386 TLLSASSVGLCQWNFGGSEERPFLVPETKNHGVCISLAHCPSSDNIVSTFRPKVEMSNEI 445

Query: 361 IASQAFRSTATCPMGNFVLGTHVSYKRLSSQCYQQLGYTSANVNDIRLPKSAIVDICDDT 540
             SQ   S     MG  + G+HV  KR+   CYQ+LG + ANVNDIRL +SAI+D+    
Sbjct: 446 AVSQY--SLTPSVMGQGMQGSHVLLKRVGGNCYQKLGSSCANVNDIRLQRSAIIDLQSHH 503

Query: 541 ALFVSGDEVRCEVTMQALPSLVTVQNLKLPKFPFRDVKFTH 663
            LFVSGDE+  E+ +Q LPSL   Q LK  K P RDVK++H
Sbjct: 504 PLFVSGDEITHELVLQELPSLRVSQRLKSQKHPLRDVKYSH 544


>emb|CAN68707.1| hypothetical protein VITISV_001643 [Vitis vinifera]
          Length = 514

 Score =  220 bits (561), Expect = 2e-55
 Identities = 110/221 (49%), Positives = 148/221 (66%)
 Frame = +1

Query: 1   CSWDFSCLNHAYAGLQNGMLLVFDMRQTRGPVESLVGLTCNPVHXXXXXXXXXXXXXXDK 180
           CSWD +  ++ YAGLQNGML+VFDMRQT  P+  + G T NP+H                
Sbjct: 175 CSWDLNSSHYMYAGLQNGMLMVFDMRQTARPMAEISGPTSNPIHTIQSVLHNSTLPSGVS 234

Query: 181 TVITASSVGICEWNFGGVDKRPFLVPGSENHGVCISLACSPRNNEIVASYRPKVKTTDEA 360
           T+++ASSVG+C+WNFGG ++RPFLVP ++NHGVCISLA  P +++IV+++RPKV+ ++E 
Sbjct: 235 TLLSASSVGLCQWNFGGSEERPFLVPETKNHGVCISLAHCPSSDBIVSTFRPKVEMSNEI 294

Query: 361 IASQAFRSTATCPMGNFVLGTHVSYKRLSSQCYQQLGYTSANVNDIRLPKSAIVDICDDT 540
             SQ   S     MG  + G+HV  KR+   CYQ+LG + ANVND RL +SAI+D+    
Sbjct: 295 AVSQY--SXTPSVMGQGMQGSHVLLKRVGGNCYQKLGSSCANVNDXRLXRSAIIDLQSHH 352

Query: 541 ALFVSGDEVRCEVTMQALPSLVTVQNLKLPKFPFRDVKFTH 663
            LFVSGDE+  E+ +Q LPSL   Q LK  K P RDVK++H
Sbjct: 353 PLFVSGDEITHELVLQELPSLRVSQRLKSQKHPLRDVKYSH 393


>ref|XP_002326378.1| predicted protein [Populus trichocarpa] gi|222833571|gb|EEE72048.1|
            predicted protein [Populus trichocarpa]
          Length = 681

 Score =  200 bits (508), Expect = 2e-49
 Identities = 104/221 (47%), Positives = 143/221 (64%), Gaps = 1/221 (0%)
 Frame = +1

Query: 1    CSWDFSCLNHAYAGLQNGMLLVFDMRQTRGPVESLVGLTCNPVHXXXXXXXXXXXXXXDK 180
            CSWD +C ++ YAGLQNG LLVFDMR T  PVES +G T +P+H               K
Sbjct: 441  CSWDLNCSHYVYAGLQNGSLLVFDMRITGSPVESRLGHTTDPIHTVQSLQHISTLPSGVK 500

Query: 181  TVITASSVGICEWNFGGVDKRPFLVPGSENHGVCISLACSPRNNEIVASYRPKVKTTDEA 360
            TV++ASSVG+C+WNFGG ++RPFL+P + N GVC+SLA    +++IV SYRP+V+ ++E 
Sbjct: 501  TVVSASSVGMCQWNFGGEEERPFLIPETTNQGVCVSLAYCASSDDIVVSYRPRVEMSNEV 560

Query: 361  IASQAFR-STATCPMGNFVLGTHVSYKRLSSQCYQQLGYTSANVNDIRLPKSAIVDICDD 537
            ++   F+ S AT   G  VLG+H+  KR  S  Y++LG   + V+ IRLPKS+I+D  + 
Sbjct: 561  VSQTLFKPSLAT---GQGVLGSHLHLKRAGSN-YEKLGIACSTVSGIRLPKSSIIDRQNQ 616

Query: 538  TALFVSGDEVRCEVTMQALPSLVTVQNLKLPKFPFRDVKFT 660
              LF +GDEV C + +Q LPS    Q LK  +    DVK+T
Sbjct: 617  KPLFATGDEVSCGLILQELPSFTATQRLKSHQHFIYDVKYT 657


>ref|XP_004143360.1| PREDICTED: E3 ubiquitin-protein ligase RFWD3-like [Cucumis sativus]
            gi|449482586|ref|XP_004156335.1| PREDICTED: E3
            ubiquitin-protein ligase RFWD3-like [Cucumis sativus]
          Length = 617

 Score =  190 bits (482), Expect = 3e-46
 Identities = 100/221 (45%), Positives = 146/221 (66%)
 Frame = +1

Query: 1    CSWDFSCLNHAYAGLQNGMLLVFDMRQTRGPVESLVGLTCNPVHXXXXXXXXXXXXXXDK 180
            CSWD +  +H YAGL+NG+L++FDMRQT GP++ L GLT NP+H              DK
Sbjct: 377  CSWDQNSSHHVYAGLKNGLLVMFDMRQTVGPLKILQGLTNNPIHTIHSLSSHSTVSN-DK 435

Query: 181  TVITASSVGICEWNFGGVDKRPFLVPGSENHGVCISLACSPRNNEIVASYRPKVKTTDEA 360
            +V++AS+ GIC+WNF  +++ PFL+P + N GVC SLA     +E+VASYRPKV  ++E 
Sbjct: 436  SVLSASAYGICQWNFDALEEGPFLIPET-NQGVCTSLAYCSSRDELVASYRPKVGMSNEI 494

Query: 361  IASQAFRSTATCPMGNFVLGTHVSYKRLSSQCYQQLGYTSANVNDIRLPKSAIVDICDDT 540
            + SQ   S +    G+ V G HV++K+     + +LG   AN++ IRLPKS I+D+ D  
Sbjct: 495  VYSQPSPSPSHTA-GHRVDGCHVAFKKEDHHQFSKLGSAHANIDAIRLPKSTIIDLPDCN 553

Query: 541  ALFVSGDEVRCEVTMQALPSLVTVQNLKLPKFPFRDVKFTH 663
            +LFVS D +  ++ +Q LPS  +VQ+LK+ K P RDVK+++
Sbjct: 554  SLFVSADAIMGDLILQELPSFRSVQHLKVHKHPVRDVKYSY 594