BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00012394
         (982 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003598881.1| TMV resistance protein N [Medicago truncatul...   232   1e-58
ref|XP_004136800.1| PREDICTED: TMV resistance protein N-like [Cu...   229   6e-58
ref|XP_002276927.2| PREDICTED: TMV resistance protein N-like [Vi...   224   3e-56
ref|XP_002264704.2| PREDICTED: TMV resistance protein N-like [Vi...   223   5e-56
gb|ACF19651.1| TIR-NBS-LRR RCT1-like resistance protein [Medicag...   221   2e-55

>ref|XP_003598881.1| TMV resistance protein N [Medicago truncatula]
            gi|355487929|gb|AES69132.1| TMV resistance protein N
            [Medicago truncatula]
          Length = 1270

 Score =  232 bits (591), Expect = 1e-58
 Identities = 123/327 (37%), Positives = 195/327 (59%), Gaps = 2/327 (0%)
 Frame = +3

Query: 3    PPENFRELSASFVTYAGGLPLALKVLGASLRGRTDMSFWKAKYEKVKQIPENEIHKILRL 182
            P  NF +LS + V Y GGLPLA++VLG+ L  RT    WK+   K+++IP  E+ + L++
Sbjct: 487  PRANFSDLSKNVVNYCGGLPLAVEVLGSYLFERTKEE-WKSVLSKLEKIPHEEVQEKLKI 545

Query: 183  SYDELADETEKAIFLDIVFFFVGKDKDEAVHIFRSCNFFPHVGITILVDKCLLTIDQNNK 362
            SYD L D+T+KAIFLD+  FF+GKD+D    I   C  F  +GI +L+++ LL +++NNK
Sbjct: 546  SYDGLTDDTKKAIFLDVCCFFIGKDRDYVTEILNGCGLFAGIGIAVLIERSLLKVEKNNK 605

Query: 363  FQMHNLIQEMGREVIREESKH--GNCRRMYLCEENECRALQNLKGADNIEGLIFDLNTWE 536
              MH+LI++MGRE++R  S +  G   R++L E+      +N  G   +EGLI +L +  
Sbjct: 606  LGMHDLIRDMGREIVRGSSTNDPGERSRLWLHEDAHSVLTKN-TGTQKVEGLILNLQSKG 664

Query: 537  KKYISAKVFERLPNLRLLEVIGAHDIRGNYKNSFHELRCIRWYHCPWTCLPSTFRPQKLV 716
            +   S  VF+++ N+RLL+ +   D+ G + +   +LR + W    + C+P  F    LV
Sbjct: 665  RDSFSTNVFQQMQNMRLLQ-LDCVDLTGEFAHLSKQLRWVNWQRSTFNCIPKDFYQGNLV 723

Query: 717  SLDMPFNQFEMLWQTSKLTGNIKQPLANLISINMSYSTNLKETPSFKGLNMVEKLCFRGC 896
             L++ F+  + +W+ +KL   +K        +N+S+S  LK TP F  L  +EKL  + C
Sbjct: 724  VLELKFSNVKQVWKETKLLDKLK-------ILNLSHSKYLKSTPDFSKLPNLEKLIMKDC 776

Query: 897  ESLQKLHPSIGHLINLGDLDMGECNRL 977
             SL ++HPSIG L  L  +++ +C  L
Sbjct: 777  PSLSEIHPSIGVLKKLLLINLKDCTSL 803


>ref|XP_004136800.1| PREDICTED: TMV resistance protein N-like [Cucumis sativus]
          Length = 996

 Score =  229 bits (585), Expect = 6e-58
 Identities = 124/326 (38%), Positives = 188/326 (57%), Gaps = 1/326 (0%)
 Frame = +3

Query: 3    PPENFRELSASFVTYAGGLPLALKVLGASLRGRTDMSFWKAKYEKVKQIPENEIHKILRL 182
            P E F +LS   +TY GGLPLAL+VLG+ L GR+    W+   +K+K+IP ++I K L++
Sbjct: 367  PSETFHQLSKQVITYCGGLPLALEVLGSFLFGRSREE-WEDTLKKLKKIPNDQIQKKLKI 425

Query: 183  SYDELADETEKAIFLDIVFFFVGKDKDEAVHIFRSCNFFPHVGITILVDKCLLTIDQNNK 362
            S+D L D T K IFLD+  FF+G +++    I   C FFP +GI++L+ +CLLTI   N+
Sbjct: 426  SFDGLNDHTYKDIFLDVSCFFIGMERNYVEQILDGCGFFPRIGISVLLQRCLLTIGDKNR 485

Query: 363  FQMHNLIQEMGREVIREE-SKHGNCRRMYLCEENECRALQNLKGADNIEGLIFDLNTWEK 539
              MH+L+++MGRE++RE   K+          E     L   KG D  EGL   L  + K
Sbjct: 486  LMMHDLLRDMGREIVRENFPKYPERHSRLFLHEEVLSVLTRQKGTDATEGLSLKLPRFSK 545

Query: 540  KYISAKVFERLPNLRLLEVIGAHDIRGNYKNSFHELRCIRWYHCPWTCLPSTFRPQKLVS 719
            + +S K F  +  LRLL+ +   D+ G++K+   E+R + W+  P   LP  F   KLV+
Sbjct: 546  QKLSTKAFNEMQKLRLLQ-LNFVDVNGDFKHISEEIRWVCWHGFPLKFLPKEFHMDKLVA 604

Query: 720  LDMPFNQFEMLWQTSKLTGNIKQPLANLISINMSYSTNLKETPSFKGLNMVEKLCFRGCE 899
            +D+ ++Q    W+ SK        L NL  +N+ +S  L  TP+F  L  +E L  + C+
Sbjct: 605  MDLRYSQIRFFWKESKF-------LKNLKFLNLGHSHYLTHTPNFSKLPNLEILSLKDCK 657

Query: 900  SLQKLHPSIGHLINLGDLDMGECNRL 977
            +L +LHP+IG L  L  L++ +C  L
Sbjct: 658  NLIELHPTIGELKALISLNLKDCKSL 683


>ref|XP_002276927.2| PREDICTED: TMV resistance protein N-like [Vitis vinifera]
          Length = 1133

 Score =  224 bits (570), Expect = 3e-56
 Identities = 132/340 (38%), Positives = 196/340 (57%), Gaps = 14/340 (4%)
 Frame = +3

Query: 3    PPENFRELSASFVTYAGGLPLALKVLGASLRGRTDMSFWKAKYEKVKQIPENEIHKILRL 182
            P +++ ELS   V YA GLPLAL+VLG+ L  ++    W+++ +K+K+IP+ EI  +LR+
Sbjct: 394  PIDDYVELSQCIVVYAQGLPLALRVLGSFLFDKSKRQ-WESQLDKLKKIPKKEIQDVLRV 452

Query: 183  SYDELADETEKAIFLDIVFFFVGKDKDEAVHIFRSCNFFPHVGITILVDKCLLTIDQNNK 362
            S+D L D  E+ IFLDI  FF G DKD  + IFRSC FFP +GI +L++K L+++ + NK
Sbjct: 453  SFDGLEDN-ERDIFLDIACFFQGHDKDYVMEIFRSCGFFPDIGIRVLIEKSLISVVE-NK 510

Query: 363  FQMHNLIQEMGREVIREES-KHGNCRRMYLCEENECRALQNLKGADNIEGLIFDLNTWEK 539
              MHNL+Q+MGRE++RE S K    R      ++    L    G + +EG+  DL++ ++
Sbjct: 511  LMMHNLLQKMGREIVREASPKEPGKRSRLWIHDDVNHVLTKKTGTEEVEGISLDLSSLKE 570

Query: 540  KYISAKVFERLPNLRLLEV-------------IGAHDIRGNYKNSFHELRCIRWYHCPWT 680
               + + F  +  LRLL+V                H  RG +K    ELR + WY  P  
Sbjct: 571  INFTNEAFAPMNRLRLLKVYTLNFLMDSKREKCKVHFSRG-FKFHCEELRHLYWYEYPLK 629

Query: 681  CLPSTFRPQKLVSLDMPFNQFEMLWQTSKLTGNIKQPLANLISINMSYSTNLKETPSFKG 860
             LP+ F  + LV L MP++Q + LW+ +K+       L NL  +N+ +S  L ETP F  
Sbjct: 630  SLPNDFNLKNLVDLSMPYSQIKQLWKGTKV-------LENLKFMNLKHSKFLTETPDFSR 682

Query: 861  LNMVEKLCFRGCESLQKLHPSIGHLINLGDLDMGECNRLK 980
            +  +E+L  +GC SL K+HPS+G L  L  L +  C  LK
Sbjct: 683  VTNLERLVLKGCISLYKVHPSLGDLNKLNFLSLKNCKMLK 722


>ref|XP_002264704.2| PREDICTED: TMV resistance protein N-like [Vitis vinifera]
          Length = 1254

 Score =  223 bits (568), Expect = 5e-56
 Identities = 132/338 (39%), Positives = 194/338 (57%), Gaps = 12/338 (3%)
 Frame = +3

Query: 3    PPENFRELSASFVTYAGGLPLALKVLGASLRGRTDMSFWKAKYEKVKQIPENEIHKILRL 182
            PP+N+ +LS   + YA GLPLALKVLG+ L G T +  WK+  +K+K  P  EIH +LR+
Sbjct: 379  PPKNYEDLSNCVINYAKGLPLALKVLGSFLYGMT-IDQWKSALDKLKGKPNMEIHNVLRI 437

Query: 183  SYDELADETEKAIFLDIVFFFVGKDKDEAVHIFRSCNFFPHVGITILVDKCLLTIDQNNK 362
            S+D L D TEK IFLDI  FF G+DKD    I   CNFF ++G+ IL D+CL+TI  N+K
Sbjct: 438  SFDGL-DHTEKQIFLDIACFFKGEDKDFISRILDGCNFFANIGLKILCDRCLITI-SNSK 495

Query: 363  FQMHNLIQEMGREVIREESKHGNCRRMYLCEENEC-RALQNLKGADNIEGLIFDLNTWEK 539
              MH+LIQ+MG+E++RE+      +   L + ++  RA    +G   IE +  D +  ++
Sbjct: 496  IHMHDLIQQMGQEIVREKYPDDPNKWSRLWDPDDIYRAFLRKEGMKKIEAISLDFSRLKE 555

Query: 540  KYISAKVFERLPNLRLLEVIGAHD-----------IRGNYKNSFHELRCIRWYHCPWTCL 686
              +S KVF R+  LRLL+V  +             I  +++   HELR + W      CL
Sbjct: 556  IQLSTKVFSRMKKLRLLKVYWSDHSSFTKKESKVFIPKDFEIPSHELRYLYWEGYSLNCL 615

Query: 687  PSTFRPQKLVSLDMPFNQFEMLWQTSKLTGNIKQPLANLISINMSYSTNLKETPSFKGLN 866
            PS F  + LV L++ ++  + LW+ SK        L  L  IN+S+S  L +   F G+ 
Sbjct: 616  PSNFHGENLVELELRYSTIKRLWKGSK-------GLEKLKFINLSHSEKLTKISKFSGMP 668

Query: 867  MVEKLCFRGCESLQKLHPSIGHLINLGDLDMGECNRLK 980
             +E+L   GC SL+K+H S+G L  L  L + +C +L+
Sbjct: 669  NLERLNLEGCTSLRKVHSSLGVLKKLTSLQLKDCQKLE 706


>gb|ACF19651.1| TIR-NBS-LRR RCT1-like resistance protein [Medicago sativa]
          Length = 1125

 Score =  221 bits (564), Expect = 2e-55
 Identities = 121/323 (37%), Positives = 189/323 (58%), Gaps = 1/323 (0%)
 Frame = +3

Query: 3    PPENFRELSASFVTYAGGLPLALKVLGASLRGRTDMSFWKAKYEKVKQIPENEIHKILRL 182
            P E+F E+S + V Y+GGLPLAL+VLG+ L  R  +  W    EK+K IP +++H+ L++
Sbjct: 418  PTEDFSEISKNVVMYSGGLPLALEVLGSYLFDREVLE-WVCVLEKLKIIPNHQLHEKLKI 476

Query: 183  SYDELADETEKAIFLDIVFFFVGKDKDEAVHIFRSCNFFPHVGITILVDKCLLTIDQNNK 362
            SYD L D+TEK+ FLDI  FF+G D+++ + I   C FF  +GI++LV++ L+T+D  NK
Sbjct: 477  SYDGLNDDTEKSTFLDIACFFIGMDRNDVIQILNGCGFFAEIGISVLVERSLVTVDDKNK 536

Query: 363  FQMHNLIQEMGREVIREESKHGNCRRMYL-CEENECRALQNLKGADNIEGLIFDLNTWEK 539
              MH+L+++MGRE+IRE+S      R  L  +E+    L    G   +EGL   L     
Sbjct: 537  LGMHDLLRDMGREIIREKSPMEPEERSRLWFQEDVLDVLSEHTGTKAVEGLTLKLPGHNA 596

Query: 540  KYISAKVFERLPNLRLLEVIGAHDIRGNYKNSFHELRCIRWYHCPWTCLPSTFRPQKLVS 719
            +  S K FE +  LRLL++ G   + G++K     LR + W   P TCLPS F  + +VS
Sbjct: 597  QRFSTKAFENMKKLRLLQLSGV-QLDGDFKYLSRNLRWLHWNGFPLTCLPSNFYQRNIVS 655

Query: 720  LDMPFNQFEMLWQTSKLTGNIKQPLANLISINMSYSTNLKETPSFKGLNMVEKLCFRGCE 899
            +++  +  ++LW+         Q +  L  +N+S+S  L +TP F  +  +EKL  + C 
Sbjct: 656  IELENSNVKLLWKE-------MQRMEQLKILNLSHSHYLTQTPDFSNMPNLEKLILKDCP 708

Query: 900  SLQKLHPSIGHLINLGDLDMGEC 968
             L ++  SIGHL  +  + + +C
Sbjct: 709  RLSEVSQSIGHLKKVLLISLKDC 731