BLASTX nr result
ID: Bupleurum21_contig00012173
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00012173
(1300 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002271955.1| PREDICTED: uncharacterized membrane protein ... 416 e-116
ref|XP_003556197.1| PREDICTED: uncharacterized membrane protein ... 393 e-109
ref|XP_002325990.1| predicted protein [Populus trichocarpa] gi|2... 394 e-109
ref|NP_001241089.1| uncharacterized protein LOC100789661 [Glycin... 390 e-108
ref|XP_002327243.1| predicted protein [Populus trichocarpa] gi|2... 385 e-106
>ref|XP_002271955.1| PREDICTED: uncharacterized membrane protein At4g09580 [Vitis
vinifera] gi|296081147|emb|CBI18173.3| unnamed protein
product [Vitis vinifera]
Length = 279
Score = 416 bits (1070), Expect(2) = e-116
Identities = 205/264 (77%), Positives = 233/264 (88%), Gaps = 2/264 (0%)
Frame = +3
Query: 273 MAAPRNVILDTSRLVIDEENV--EDSPTTKKPIGAKLEGKFPVNRWEXXXXXXXXXXXST 446
MAAPRN+ D RL DEEN +DSPTTKKP G K++G+FP+ RWE S
Sbjct: 1 MAAPRNLGNDGGRLR-DEENANEDDSPTTKKPAGVKIDGRFPLTRWEFTVALGVFVFFSI 59
Query: 447 GLFCIYLTMPAAEYGKLKLPRTVADLRLLKEHLAMYADHYPAKFILGYCATYIFMQTFMI 626
GLFCIYLTMPAAEYG+++LPRT++DLR+LK+ LA YA+ YPA+FI+GYC+TYIFMQTFMI
Sbjct: 60 GLFCIYLTMPAAEYGRIRLPRTISDLRMLKDQLATYANDYPAQFIMGYCSTYIFMQTFMI 119
Query: 627 PGTIFMSLLAGALFGVIKGIFLVVFNATAGASSCYFLSKLIGKPIVIWLWPEKLKFFQGE 806
PGTIFMSLLAGALFGV++G+FLVVFNATAGASSCYFLSKLIG+P+V WLWPEKL+ FQ E
Sbjct: 120 PGTIFMSLLAGALFGVVRGLFLVVFNATAGASSCYFLSKLIGRPLVNWLWPEKLRLFQAE 179
Query: 807 IAKRRDKLLNYMLFLRITPTLPNLFINLASPIMDIPFHVFFTATVIGLIPASYITVRAGL 986
I+KRR+KLLNYMLFLRITPTLPNLFINLASPI+DIPFHVFF AT++GLIPASYITVRAGL
Sbjct: 180 ISKRREKLLNYMLFLRITPTLPNLFINLASPIVDIPFHVFFLATLVGLIPASYITVRAGL 239
Query: 987 ALGDLKSVKDLYDFKTLSVLFLIG 1058
ALGDLKSVKDLYDFKTLSVLFLIG
Sbjct: 240 ALGDLKSVKDLYDFKTLSVLFLIG 263
Score = 30.8 bits (68), Expect(2) = e-116
Identities = 13/20 (65%), Positives = 16/20 (80%)
Frame = +2
Query: 1055 WFLTFFSILPTILKRKRIYE 1114
+ + F SILPT+LKRKR YE
Sbjct: 260 FLIGFISILPTLLKRKRTYE 279
>ref|XP_003556197.1| PREDICTED: uncharacterized membrane protein At4g09580-like [Glycine
max]
Length = 271
Score = 393 bits (1009), Expect(2) = e-109
Identities = 200/264 (75%), Positives = 225/264 (85%), Gaps = 2/264 (0%)
Frame = +3
Query: 273 MAAPRNVILDTSRLVIDEENVED--SPTTKKPIGAKLEGKFPVNRWEXXXXXXXXXXXST 446
MAAPRNV DEE ED SP+ KKP K E +FP+N WE ST
Sbjct: 1 MAAPRNVTATR-----DEEKGEDDDSPSAKKP---KSE-RFPLNTWEFAVAVAVFLVFST 51
Query: 447 GLFCIYLTMPAAEYGKLKLPRTVADLRLLKEHLAMYADHYPAKFILGYCATYIFMQTFMI 626
GLFCIYLTMP+A Y LKLPRT++DLRLLKEHL+ YA ++PA+FILGYC+TYIFMQTFMI
Sbjct: 52 GLFCIYLTMPSAAYTNLKLPRTLSDLRLLKEHLSTYASNHPAQFILGYCSTYIFMQTFMI 111
Query: 627 PGTIFMSLLAGALFGVIKGIFLVVFNATAGASSCYFLSKLIGKPIVIWLWPEKLKFFQGE 806
PGTIFMSLLAGALFGV++GI LVVFNATAGASSC+FLSKLIG+P+V WLWPEKL+FFQ E
Sbjct: 112 PGTIFMSLLAGALFGVVRGILLVVFNATAGASSCFFLSKLIGRPLVSWLWPEKLRFFQAE 171
Query: 807 IAKRRDKLLNYMLFLRITPTLPNLFINLASPIMDIPFHVFFTATVIGLIPASYITVRAGL 986
IAKRRD+LLNYMLFLRITPTLPNLFINLASPI+D+PFH+FF+AT+IGL+PASYITVRAGL
Sbjct: 172 IAKRRDRLLNYMLFLRITPTLPNLFINLASPIVDVPFHIFFSATLIGLVPASYITVRAGL 231
Query: 987 ALGDLKSVKDLYDFKTLSVLFLIG 1058
ALGDLKS+KDLYDFKTLSVLFLIG
Sbjct: 232 ALGDLKSIKDLYDFKTLSVLFLIG 255
Score = 30.0 bits (66), Expect(2) = e-109
Identities = 12/20 (60%), Positives = 16/20 (80%)
Frame = +2
Query: 1055 WFLTFFSILPTILKRKRIYE 1114
+ + F SI PT+LKRKR+YE
Sbjct: 252 FLIGFVSIAPTLLKRKRVYE 271
>ref|XP_002325990.1| predicted protein [Populus trichocarpa] gi|222862865|gb|EEF00372.1|
predicted protein [Populus trichocarpa]
Length = 262
Score = 394 bits (1013), Expect(2) = e-109
Identities = 196/248 (79%), Positives = 221/248 (89%), Gaps = 2/248 (0%)
Frame = +3
Query: 321 DEENV--EDSPTTKKPIGAKLEGKFPVNRWEXXXXXXXXXXXSTGLFCIYLTMPAAEYGK 494
DEE +DSP+ K+ AK E +FP+ RWE STGLFCIYLTMPAAEYGK
Sbjct: 3 DEEKAKEDDSPSAKR---AKFE-RFPLTRWELAAALGVFFVFSTGLFCIYLTMPAAEYGK 58
Query: 495 LKLPRTVADLRLLKEHLAMYADHYPAKFILGYCATYIFMQTFMIPGTIFMSLLAGALFGV 674
LKLPRT++DLRLLK++LA YA+ YPA+FILGYC+TYIFMQTFMIPGTIFMSLLAGALFGV
Sbjct: 59 LKLPRTISDLRLLKDNLATYANEYPAQFILGYCSTYIFMQTFMIPGTIFMSLLAGALFGV 118
Query: 675 IKGIFLVVFNATAGASSCYFLSKLIGKPIVIWLWPEKLKFFQGEIAKRRDKLLNYMLFLR 854
++GIFLVVFNATAGASSC+FLSKLIG+P+V WLWPEKL+FFQ EIAKRRDKLLNYMLFLR
Sbjct: 119 VRGIFLVVFNATAGASSCFFLSKLIGRPLVNWLWPEKLRFFQAEIAKRRDKLLNYMLFLR 178
Query: 855 ITPTLPNLFINLASPIMDIPFHVFFTATVIGLIPASYITVRAGLALGDLKSVKDLYDFKT 1034
+TPTLPNLFINLASPI+DIPFH+FF AT++GLIPASYITVRAGLALGDLKSVKDLYD+KT
Sbjct: 179 VTPTLPNLFINLASPIVDIPFHIFFLATLLGLIPASYITVRAGLALGDLKSVKDLYDYKT 238
Query: 1035 LSVLFLIG 1058
LSVLF+IG
Sbjct: 239 LSVLFIIG 246
Score = 28.5 bits (62), Expect(2) = e-109
Identities = 12/14 (85%), Positives = 13/14 (92%)
Frame = +2
Query: 1073 SILPTILKRKRIYE 1114
SI PT+LKRKRIYE
Sbjct: 249 SIFPTLLKRKRIYE 262
>ref|NP_001241089.1| uncharacterized protein LOC100789661 [Glycine max]
gi|255639798|gb|ACU20192.1| unknown [Glycine max]
Length = 271
Score = 390 bits (1001), Expect(2) = e-108
Identities = 197/264 (74%), Positives = 225/264 (85%), Gaps = 2/264 (0%)
Frame = +3
Query: 273 MAAPRNVILDTSRLVIDEENVE--DSPTTKKPIGAKLEGKFPVNRWEXXXXXXXXXXXST 446
MAAPRNV DEE E DSP+ KKP K E +FP+N WE ST
Sbjct: 1 MAAPRNVTATR-----DEEKGEEDDSPSAKKP---KSE-RFPLNTWEFAVAVAVFFVFST 51
Query: 447 GLFCIYLTMPAAEYGKLKLPRTVADLRLLKEHLAMYADHYPAKFILGYCATYIFMQTFMI 626
GLFC+YL++P+A Y LKLPRT++DLRLLKEHL+ YA ++PA+FILGYC+TYIFMQTFMI
Sbjct: 52 GLFCVYLSLPSAAYTNLKLPRTLSDLRLLKEHLSAYASNHPAQFILGYCSTYIFMQTFMI 111
Query: 627 PGTIFMSLLAGALFGVIKGIFLVVFNATAGASSCYFLSKLIGKPIVIWLWPEKLKFFQGE 806
PGTIFMSLLAGALFGV++GI LVVFNATAGASSC+FLSKLIG+P+V WLWPEKL+FFQ E
Sbjct: 112 PGTIFMSLLAGALFGVVRGILLVVFNATAGASSCFFLSKLIGRPLVSWLWPEKLRFFQAE 171
Query: 807 IAKRRDKLLNYMLFLRITPTLPNLFINLASPIMDIPFHVFFTATVIGLIPASYITVRAGL 986
IAKRRD+LLNYMLFLRITPTLPNLFINLASPI+D+PFH+FF+AT+IGL+PASYITVRAGL
Sbjct: 172 IAKRRDRLLNYMLFLRITPTLPNLFINLASPIVDVPFHIFFSATLIGLVPASYITVRAGL 231
Query: 987 ALGDLKSVKDLYDFKTLSVLFLIG 1058
ALGDLKS+KDLYDFKTLSVLFLIG
Sbjct: 232 ALGDLKSIKDLYDFKTLSVLFLIG 255
Score = 30.0 bits (66), Expect(2) = e-108
Identities = 12/20 (60%), Positives = 16/20 (80%)
Frame = +2
Query: 1055 WFLTFFSILPTILKRKRIYE 1114
+ + F SI PT+LKRKR+YE
Sbjct: 252 FLIGFVSIAPTLLKRKRVYE 271
>ref|XP_002327243.1| predicted protein [Populus trichocarpa] gi|222835613|gb|EEE74048.1|
predicted protein [Populus trichocarpa]
Length = 262
Score = 385 bits (988), Expect(2) = e-106
Identities = 188/248 (75%), Positives = 219/248 (88%), Gaps = 2/248 (0%)
Frame = +3
Query: 321 DEENV--EDSPTTKKPIGAKLEGKFPVNRWEXXXXXXXXXXXSTGLFCIYLTMPAAEYGK 494
DEE +DSP+ K+ +K E +FP+ RWE STGLFCIYLTMPAA+YGK
Sbjct: 3 DEEKAKEDDSPSAKR---SKFE-RFPLTRWELAAALGVFLVFSTGLFCIYLTMPAADYGK 58
Query: 495 LKLPRTVADLRLLKEHLAMYADHYPAKFILGYCATYIFMQTFMIPGTIFMSLLAGALFGV 674
LKLPR+++DLRLLK++LA Y YPA+FILGYC+TYIFMQTFMIPGTIFMSLLAGALFG+
Sbjct: 59 LKLPRSISDLRLLKDNLATYVSEYPAQFILGYCSTYIFMQTFMIPGTIFMSLLAGALFGI 118
Query: 675 IKGIFLVVFNATAGASSCYFLSKLIGKPIVIWLWPEKLKFFQGEIAKRRDKLLNYMLFLR 854
++G+FLVVFNATAGASSC+FLSKLIG+P+V WLWPEK++FFQ EIAKR++KLLNYMLFLR
Sbjct: 119 VRGLFLVVFNATAGASSCFFLSKLIGRPLVNWLWPEKMRFFQSEIAKRKEKLLNYMLFLR 178
Query: 855 ITPTLPNLFINLASPIMDIPFHVFFTATVIGLIPASYITVRAGLALGDLKSVKDLYDFKT 1034
+TPTLPNLFINLASPI+DIPFH+FF AT++GLIPASYITV+AGLALGDLKSVKDLYDFKT
Sbjct: 179 VTPTLPNLFINLASPIVDIPFHIFFLATLLGLIPASYITVKAGLALGDLKSVKDLYDFKT 238
Query: 1035 LSVLFLIG 1058
LSVLFLIG
Sbjct: 239 LSVLFLIG 246
Score = 28.5 bits (62), Expect(2) = e-106
Identities = 12/14 (85%), Positives = 13/14 (92%)
Frame = +2
Query: 1073 SILPTILKRKRIYE 1114
SI PT+LKRKRIYE
Sbjct: 249 SIFPTLLKRKRIYE 262