BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00011971
         (1719 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI38934.3| unnamed protein product [Vitis vinifera]              332   e-140
ref|XP_002273145.2| PREDICTED: GPI ethanolamine phosphate transf...   332   e-140
ref|XP_002530304.1| phosphatidylinositol glycan, putative [Ricin...   315   e-130
ref|XP_003541326.1| PREDICTED: GPI ethanolamine phosphate transf...   303   e-124
ref|XP_004135734.1| PREDICTED: GPI ethanolamine phosphate transf...   290   e-123

>emb|CBI38934.3| unnamed protein product [Vitis vinifera]
          Length = 941

 Score =  332 bits (851), Expect(2) = e-140
 Identities = 175/305 (57%), Positives = 224/305 (73%), Gaps = 2/305 (0%)
 Frame = -3

Query: 1717 HGQTLNGDHGGGSPEEVETSIFAMSMQPPPSA-STEQDSSYCKLDTAGRNVCINFIEQLD 1541
            HGQT+NGDHGGG+ EEVETSIFAMS++  PS+   E ++S C+L     N+CIN I+QLD
Sbjct: 292  HGQTINGDHGGGTAEEVETSIFAMSLKTTPSSLPLELNTSCCELHLDKENMCINSIQQLD 351

Query: 1540 FAVTVSALLGVPSPYGSIGCINPELYALVPGTWNRESYNEGNSQ-QIDTEAWMCNYVDAL 1364
            FAVTVSA+LG+P P+GSIG +N ELYAL  GTWN ES N GN Q Q++ ++WM +YV+ L
Sbjct: 352  FAVTVSAMLGIPFPFGSIGRVNSELYALGSGTWNLESINVGNRQTQLNMQSWMHDYVNVL 411

Query: 1363 CINSWQVKKYIDVYSASSVIGFSKDDLLLVEEMYAQAEKGRSNTVNNSVHYKGDSTHISV 1184
            CINSWQVK+YIDVYSASS+IGFS +D++ + ++YAQAE+  S+T+ N +  K +S + ++
Sbjct: 412  CINSWQVKRYIDVYSASSIIGFSSEDIMRITDIYAQAEESWSHTIKNLLLDKNESCN-TM 470

Query: 1183 NALKRQIDLYDKFLLSVAELARTKWTEFNLEKMAFGFGVMLTSIVIHFLVIKRLDDLQKA 1004
              +KRQID Y  FL SVAELAR+KWTEF+L+ M  G G+ML ++VI FL IKR++     
Sbjct: 471  LPIKRQIDAYSDFLASVAELARSKWTEFDLKMMGVGLGIMLITLVIQFLGIKRMNKTCGV 530

Query: 1003 XXXXXXXXXXSVNAVFAYLLVAIRACSVLSNSYILEEGKVAVFLLATTAILYLRYSIQNK 824
                      S   +F+  +V IRACS LSNSYILEEGKVA FLLATT IL  R SI  K
Sbjct: 531  NFPSPGDSWTSFGLIFSIFIVMIRACSFLSNSYILEEGKVASFLLATTGILKFRNSILKK 590

Query: 823  KMLME 809
            KML+E
Sbjct: 591  KMLLE 595



 Score =  194 bits (492), Expect(2) = e-140
 Identities = 114/238 (47%), Positives = 139/238 (58%), Gaps = 5/238 (2%)
 Frame = -1

Query: 699  RFTIEFGLSKQATNSTLITVHLSRIVRIDKDSFVWMYIANIVPIXXXXXXXXXXXXXXAH 520
            R TIE GLSKQA +S   ++ L            WMYIA IVP+                
Sbjct: 607  RLTIELGLSKQAFSSGFTSIPL------------WMYIAEIVPMLALVLLACFLYKSIDD 654

Query: 519  RNCQIIMRYVINGTIFSYILIGVHWILENDLIS--LSHIGIKTDFIPRTIYAIGLAQLLS 346
              C  ++++VI  TI SY+LI VHW +E++L+   L   G     IPR IYAIG+ QLL 
Sbjct: 655  TACVGLLKFVIAVTILSYLLIAVHWTMESNLVGTPLMLQGTGKGLIPRIIYAIGIGQLLI 714

Query: 345  LALIR---EEKLSGWKDSIVFKTVTMLSAWSSTIIVLSGKQGVLVALASIAAGWLILRLQ 175
            LAL R   +EK    K  ++ K V MLSAWSST+I++SGKQG LVALASI  GW I+RL+
Sbjct: 715  LALGRLFGKEKALDSKKGLILKVVAMLSAWSSTVIIVSGKQGPLVALASIVGGWCIMRLE 774

Query: 174  SLDQXXXXXXXXXXXXXXXXXTQWSLLAACLFFGTGHWCAFDGLRYAAAFIGFDEFNL 1
            +L+                  TQWSLLA  LFF TGHWCAFDGLRY AAFIGFD+F L
Sbjct: 775  NLEHESRDGSVGVLNLSPLPVTQWSLLAVSLFFCTGHWCAFDGLRYGAAFIGFDDFIL 832


>ref|XP_002273145.2| PREDICTED: GPI ethanolamine phosphate transferase 3-like [Vitis
            vinifera]
          Length = 928

 Score =  332 bits (851), Expect(2) = e-140
 Identities = 175/305 (57%), Positives = 224/305 (73%), Gaps = 2/305 (0%)
 Frame = -3

Query: 1717 HGQTLNGDHGGGSPEEVETSIFAMSMQPPPSA-STEQDSSYCKLDTAGRNVCINFIEQLD 1541
            HGQT+NGDHGGG+ EEVETSIFAMS++  PS+   E ++S C+L     N+CIN I+QLD
Sbjct: 279  HGQTINGDHGGGTAEEVETSIFAMSLKTTPSSLPLELNTSCCELHLDKENMCINSIQQLD 338

Query: 1540 FAVTVSALLGVPSPYGSIGCINPELYALVPGTWNRESYNEGNSQ-QIDTEAWMCNYVDAL 1364
            FAVTVSA+LG+P P+GSIG +N ELYAL  GTWN ES N GN Q Q++ ++WM +YV+ L
Sbjct: 339  FAVTVSAMLGIPFPFGSIGRVNSELYALGSGTWNLESINVGNRQTQLNMQSWMHDYVNVL 398

Query: 1363 CINSWQVKKYIDVYSASSVIGFSKDDLLLVEEMYAQAEKGRSNTVNNSVHYKGDSTHISV 1184
            CINSWQVK+YIDVYSASS+IGFS +D++ + ++YAQAE+  S+T+ N +  K +S + ++
Sbjct: 399  CINSWQVKRYIDVYSASSIIGFSSEDIMRITDIYAQAEESWSHTIKNLLLDKNESCN-TM 457

Query: 1183 NALKRQIDLYDKFLLSVAELARTKWTEFNLEKMAFGFGVMLTSIVIHFLVIKRLDDLQKA 1004
              +KRQID Y  FL SVAELAR+KWTEF+L+ M  G G+ML ++VI FL IKR++     
Sbjct: 458  LPIKRQIDAYSDFLASVAELARSKWTEFDLKMMGVGLGIMLITLVIQFLGIKRMNKTCGV 517

Query: 1003 XXXXXXXXXXSVNAVFAYLLVAIRACSVLSNSYILEEGKVAVFLLATTAILYLRYSIQNK 824
                      S   +F+  +V IRACS LSNSYILEEGKVA FLLATT IL  R SI  K
Sbjct: 518  NFPSPGDSWTSFGLIFSIFIVMIRACSFLSNSYILEEGKVASFLLATTGILKFRNSILKK 577

Query: 823  KMLME 809
            KML+E
Sbjct: 578  KMLLE 582



 Score =  194 bits (492), Expect(2) = e-140
 Identities = 114/238 (47%), Positives = 139/238 (58%), Gaps = 5/238 (2%)
 Frame = -1

Query: 699  RFTIEFGLSKQATNSTLITVHLSRIVRIDKDSFVWMYIANIVPIXXXXXXXXXXXXXXAH 520
            R TIE GLSKQA +S   ++ L            WMYIA IVP+                
Sbjct: 594  RLTIELGLSKQAFSSGFTSIPL------------WMYIAEIVPMLALVLLACFLYKSIDD 641

Query: 519  RNCQIIMRYVINGTIFSYILIGVHWILENDLIS--LSHIGIKTDFIPRTIYAIGLAQLLS 346
              C  ++++VI  TI SY+LI VHW +E++L+   L   G     IPR IYAIG+ QLL 
Sbjct: 642  TACVGLLKFVIAVTILSYLLIAVHWTMESNLVGTPLMLQGTGKGLIPRIIYAIGIGQLLI 701

Query: 345  LALIR---EEKLSGWKDSIVFKTVTMLSAWSSTIIVLSGKQGVLVALASIAAGWLILRLQ 175
            LAL R   +EK    K  ++ K V MLSAWSST+I++SGKQG LVALASI  GW I+RL+
Sbjct: 702  LALGRLFGKEKALDSKKGLILKVVAMLSAWSSTVIIVSGKQGPLVALASIVGGWCIMRLE 761

Query: 174  SLDQXXXXXXXXXXXXXXXXXTQWSLLAACLFFGTGHWCAFDGLRYAAAFIGFDEFNL 1
            +L+                  TQWSLLA  LFF TGHWCAFDGLRY AAFIGFD+F L
Sbjct: 762  NLEHESRDGSVGVLNLSPLPVTQWSLLAVSLFFCTGHWCAFDGLRYGAAFIGFDDFIL 819


>ref|XP_002530304.1| phosphatidylinositol glycan, putative [Ricinus communis]
            gi|223530160|gb|EEF32071.1| phosphatidylinositol glycan,
            putative [Ricinus communis]
          Length = 967

 Score =  315 bits (808), Expect(2) = e-130
 Identities = 168/304 (55%), Positives = 213/304 (70%), Gaps = 1/304 (0%)
 Frame = -3

Query: 1717 HGQTLNGDHGGGSPEEVETSIFAMSMQPPP-SASTEQDSSYCKLDTAGRNVCINFIEQLD 1541
            HGQTLNGDHGGGS EEVETSIFAMS +  P S  +E D+S C+ D  G  +C + + QLD
Sbjct: 294  HGQTLNGDHGGGSAEEVETSIFAMSSKRQPFSIPSELDTSSCEQDLDGNEICTSSLHQLD 353

Query: 1540 FAVTVSALLGVPSPYGSIGCINPELYALVPGTWNRESYNEGNSQQIDTEAWMCNYVDALC 1361
            FAVT+SALLGV  P+GSIG +NPELYAL  GTWN E    G+ +    E W+ NYV+ LC
Sbjct: 354  FAVTLSALLGVSFPFGSIGRVNPELYALGSGTWNLEETKVGDCKLSKLEDWVQNYVNVLC 413

Query: 1360 INSWQVKKYIDVYSASSVIGFSKDDLLLVEEMYAQAEKGRSNTVNNSVHYKGDSTHISVN 1181
            INSWQVK+YIDVYSASS+IGFS +DLL + ++Y QAE+   + + + + YK +S H  + 
Sbjct: 414  INSWQVKRYIDVYSASSMIGFSSEDLLHISDVYNQAEENWLH-IKDLLSYKNESCHSLLP 472

Query: 1180 ALKRQIDLYDKFLLSVAELARTKWTEFNLEKMAFGFGVMLTSIVIHFLVIKRLDDLQKAX 1001
             L RQID Y  FL +V+ELAR+KWTEFNL+ M  G G+ML S+++ FL I++ +      
Sbjct: 473  DLLRQIDTYFNFLSNVSELARSKWTEFNLKMMGIGLGIMLMSLLVMFLAIQQANRPYAVF 532

Query: 1000 XXXXXXXXXSVNAVFAYLLVAIRACSVLSNSYILEEGKVAVFLLATTAILYLRYSIQNKK 821
                     S + VFA+ +VAIRA S+ SNSYILEEGKVA FLLATT I+ LRYSI  +K
Sbjct: 533  RPTPGNSMISFDLVFAFFIVAIRAGSLFSNSYILEEGKVASFLLATTGIIKLRYSIMKRK 592

Query: 820  MLME 809
            M+ E
Sbjct: 593  MIYE 596



 Score =  177 bits (448), Expect(2) = e-130
 Identities = 107/240 (44%), Positives = 140/240 (58%), Gaps = 7/240 (2%)
 Frame = -1

Query: 699  RFTIEFGLSKQATNSTLITVHLSRIVRIDKDSFVWMYIANIVPIXXXXXXXXXXXXXXAH 520
            RF+IE GLSKQA  S  ++   S ++ I     +WM ++ I PI              + 
Sbjct: 608  RFSIEVGLSKQAATSLFMSASPSWMLGIAPGHPIWMCMSEIGPILAIISLAWLLYRTTSS 667

Query: 519  RNCQIIMRYVINGTIFSYILIGVHWILENDLISLSHI--GIKTDFIPRTIYAIGLAQLLS 346
             +   I + +I GTI SY+LI VHW+ E+  +SL  +  GI   +IPR +Y IGL QL  
Sbjct: 668  SHYWGIWKCIIMGTISSYLLIAVHWLSESSTLSLVLLLRGIGKSYIPRLVYGIGLGQLTL 727

Query: 345  LAL----IREEKLSG-WKDSIVFKTVTMLSAWSSTIIVLSGKQGVLVALASIAAGWLILR 181
            +A      +E+ L G W  S++ KTV + SAWSSTII+LSGKQG LVALA I  G+ I +
Sbjct: 728  VAFGQCFTKEKPLDGNW--SLIIKTVALSSAWSSTIILLSGKQGSLVALAFIIGGYCIKK 785

Query: 180  LQSLDQXXXXXXXXXXXXXXXXXTQWSLLAACLFFGTGHWCAFDGLRYAAAFIGFDEFNL 1
            L++++                   QW+LLA CLFF TGHWCAFDGLRY AAFIGFDEF L
Sbjct: 786  LENMEDAISGTATMLNFNPLAVT-QWNLLAVCLFFATGHWCAFDGLRYGAAFIGFDEFIL 844


>ref|XP_003541326.1| PREDICTED: GPI ethanolamine phosphate transferase 3-like [Glycine
            max]
          Length = 949

 Score =  303 bits (775), Expect(2) = e-124
 Identities = 161/305 (52%), Positives = 206/305 (67%), Gaps = 2/305 (0%)
 Frame = -3

Query: 1717 HGQTLNGDHGGGSPEEVETSIFAMSMQPP-PSASTEQDSSYCKLDTAGRNVCINFIEQLD 1541
            HGQTLNGDHGGGS EEVET+IFAMS + P  S  +E DS  C+LD  G+NVCI+ ++QLD
Sbjct: 292  HGQTLNGDHGGGSAEEVETAIFAMSFKKPLSSVPSEFDSCSCQLDLDGKNVCISTMQQLD 351

Query: 1540 FAVTVSALLGVPSPYGSIGCINPELYALVPGTWNRESYNEGNSQQI-DTEAWMCNYVDAL 1364
            FAVTVSALLG+P PYGSIG INPELYAL   +WN ++     SQ++ +++ WM NY +AL
Sbjct: 352  FAVTVSALLGIPFPYGSIGHINPELYALGADSWNSDA-----SQKLSESDIWMQNYANAL 406

Query: 1363 CINSWQVKKYIDVYSASSVIGFSKDDLLLVEEMYAQAEKGRSNTVNNSVHYKGDSTHISV 1184
            CINSWQVK+Y+D YS SS +GFS DDL  +  +YAQ E   S++    +  + + +   V
Sbjct: 407  CINSWQVKRYVDAYSTSSAVGFSHDDLSRIASVYAQVENHWSHSTKKLLLDRQNDSDTLV 466

Query: 1183 NALKRQIDLYDKFLLSVAELARTKWTEFNLEKMAFGFGVMLTSIVIHFLVIKRLDDLQKA 1004
             ALKRQID Y KFL +V+ELAR+KWTEF+L  M  G G+ML S++     I R +     
Sbjct: 467  PALKRQIDAYFKFLTTVSELARSKWTEFDLNMMGTGIGIMLVSLIFQVFTILRANKKHGV 526

Query: 1003 XXXXXXXXXXSVNAVFAYLLVAIRACSVLSNSYILEEGKVAVFLLATTAILYLRYSIQNK 824
                         ++F   L+ IRACS LSNSYILEEGKVA FLL+T+ I+ LR S+   
Sbjct: 527  MFSSSGDSCIITGSIFTIFLLGIRACSFLSNSYILEEGKVANFLLSTSGIVTLRQSVIQG 586

Query: 823  KMLME 809
            K+L E
Sbjct: 587  KLLKE 591



 Score =  169 bits (428), Expect(2) = e-124
 Identities = 104/237 (43%), Positives = 132/237 (55%), Gaps = 4/237 (1%)
 Frame = -1

Query: 699  RFTIEFGLSKQATNSTLITVHLSRIVRIDKDSFVWMYIANIVPIXXXXXXXXXXXXXXAH 520
            RF IE GLSKQA  S  +  + S I+ I     VW Y A ++P+              + 
Sbjct: 603  RFAIEVGLSKQAATSAFMKDYTSWIINIASGLPVWDYAAEVIPMVVLILLAAWLYKATSG 662

Query: 519  RNCQIIMRYVINGTIFSYILIGVHWILENDLI--SLSHIGIKTDFIPRTIYAIGLAQLLS 346
                   +YVI GTI SY+LI VHWI ++D    +L    I   +IPR IYAI L QLL 
Sbjct: 663  SLFDWPWKYVILGTILSYMLIIVHWITDSDRFGGTLMSQNIGRTYIPRIIYAIALGQLLL 722

Query: 345  LALIREEKLSGW--KDSIVFKTVTMLSAWSSTIIVLSGKQGVLVALASIAAGWLILRLQS 172
            L   +  K S    K  +V KT+ +LSAWSST+I+LSGKQG +VA ASI  G+ I++  +
Sbjct: 723  LTFGQLFKNSSLDCKTILVAKTMAILSAWSSTVILLSGKQGPMVAFASIVGGYFIMKFVN 782

Query: 171  LDQXXXXXXXXXXXXXXXXXTQWSLLAACLFFGTGHWCAFDGLRYAAAFIGFDEFNL 1
            ++                   QWSL A CLFF +GHWCAFDGLRY AAFIGF+EF L
Sbjct: 783  VE------GGKDEPHRSFSIMQWSLFATCLFFCSGHWCAFDGLRYGAAFIGFEEFVL 833


>ref|XP_004135734.1| PREDICTED: GPI ethanolamine phosphate transferase 3-like [Cucumis
            sativus]
          Length = 955

 Score =  290 bits (742), Expect(2) = e-123
 Identities = 159/304 (52%), Positives = 201/304 (66%), Gaps = 1/304 (0%)
 Frame = -3

Query: 1717 HGQTLNGDHGGGSPEEVETSIFAMSMQP-PPSASTEQDSSYCKLDTAGRNVCINFIEQLD 1541
            HGQTLNGDHGGGS EEVETS+FAMS      S  +E  +S C+LD+ GR +C + I+QLD
Sbjct: 295  HGQTLNGDHGGGSAEEVETSLFAMSFNKLSASIPSEFGTSSCQLDSQGREICTSSIQQLD 354

Query: 1540 FAVTVSALLGVPSPYGSIGCINPELYALVPGTWNRESYNEGNSQQIDTEAWMCNYVDALC 1361
            F VT+SALLG+P PYGSIG +NPELYAL  G+   +    G S    +  WM NYV+ LC
Sbjct: 355  FPVTLSALLGIPFPYGSIGRVNPELYALGAGSMKLDGTKVG-SYLNQSGGWMQNYVNVLC 413

Query: 1360 INSWQVKKYIDVYSASSVIGFSKDDLLLVEEMYAQAEKGRSNTVNNSVHYKGDSTHISVN 1181
            +NSWQVK+YID Y+ASSVIGFS +DLL    +Y  A +  S+     +    D    ++ 
Sbjct: 414  VNSWQVKRYIDNYTASSVIGFSDEDLLHTRSLYDDAMESWSHIRKGLL--SNDDGSDNIP 471

Query: 1180 ALKRQIDLYDKFLLSVAELARTKWTEFNLEKMAFGFGVMLTSIVIHFLVIKRLDDLQKAX 1001
            +LKRQID Y  FL SVAELAR+KWTEFNL+ M  GF +ML S+ +HFL IKR+  L  + 
Sbjct: 472  SLKRQIDAYSNFLASVAELARSKWTEFNLKMMTLGFSLMLASLFVHFLAIKRISKLCSS- 530

Query: 1000 XXXXXXXXXSVNAVFAYLLVAIRACSVLSNSYILEEGKVAVFLLATTAILYLRYSIQNKK 821
                     +   + +  LVAIRACS LSNS+ILEEGK   FLLAT+ I+ LRYSI  +K
Sbjct: 531  SFANEDCGTTFELMLSCFLVAIRACSFLSNSFILEEGKATSFLLATSGIIMLRYSIAERK 590

Query: 820  MLME 809
              ++
Sbjct: 591  HFLK 594



 Score =  181 bits (460), Expect(2) = e-123
 Identities = 110/239 (46%), Positives = 140/239 (58%), Gaps = 6/239 (2%)
 Frame = -1

Query: 699  RFTIEFGLSKQATNSTLITVHLSRIVRIDKDSFVWMYIANIVPIXXXXXXXXXXXXXXAH 520
            RFTIE GL KQA  S  + V+ S ++ I      W Y+   VPI              + 
Sbjct: 606  RFTIEVGLLKQADTSAFLKVYPSWVLEIASLLPGWTYVTEAVPIIALILLVQLLLKNVSG 665

Query: 519  RNCQIIMRYVINGTIFSYILIGVHWILENDLISLSHI--GIKTDFIPRTIYAIGLAQLLS 346
               + + ++V+ GTIF YIL GVHW LEND++       GI  + +PR IYAIGL QL S
Sbjct: 666  SQSKGMWQFVVYGTIFCYILTGVHWALENDMLHFVPAVEGIGKNCLPRIIYAIGLGQL-S 724

Query: 345  LALIR----EEKLSGWKDSIVFKTVTMLSAWSSTIIVLSGKQGVLVALASIAAGWLILRL 178
            L L R    E+K    + ++V KTVTML+A S T+I+L+GKQG LVALAS+  G+ I+ +
Sbjct: 725  LLLFRQLFGEDKPLNCRKTLVTKTVTMLAACSPTVIILAGKQGSLVALASVLGGYCIISM 784

Query: 177  QSLDQXXXXXXXXXXXXXXXXXTQWSLLAACLFFGTGHWCAFDGLRYAAAFIGFDEFNL 1
             +L +                 TQWSL A CLFF +GHWCAFDGLRYAAAFIGFDEF L
Sbjct: 785  DNL-RHGGDGNDRVLTVDSLPVTQWSLFAICLFFSSGHWCAFDGLRYAAAFIGFDEFVL 842