BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00011954
         (1065 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ADO51748.1| alcohol dehydrogenase [Camellia sinensis]              401   e-132
pdb|4HFJ|A Chain A, X-ray Crystal Structure Of A Double Bond Red...   394   e-132
dbj|BAA89423.1| allyl alcohol dehydrogenase [Nicotiana tabacum]       394   e-132
ref|XP_002279359.1| PREDICTED: NADP-dependent alkenal double bon...   403   e-131
gb|ACN65116.1| 2-alkenal reductase [Artemisia annua]                  385   e-130

>gb|ADO51748.1| alcohol dehydrogenase [Camellia sinensis]
          Length = 347

 Score =  401 bits (1031), Expect(2) = e-132
 Identities = 191/230 (83%), Positives = 214/230 (93%)
 Frame = -2

Query: 812 TGWEEYSLITNVASLFKIEHTDVPLSYYTGLLGMPGMTAYAGFYEICAPKKGEYVYVSAA 633
           TGWEEY++IT  A++FKI++TDVPLSYYTG+LGM GMTAY GFYEIC+PK+G+YV++SAA
Sbjct: 107 TGWEEYTIITEPANVFKIQNTDVPLSYYTGILGMHGMTAYVGFYEICSPKQGDYVFISAA 166

Query: 632 SGAVGQLVGQFAKLLGCYVVGSAGSKEKVDLLKNKFGFDEAFNYKEEKDLAAALKRYFPD 453
           SGAVGQLVGQFAKLLGCYVVGSAG+KEKVDLLKNKFGFDEAFNYKEEKDL AALKRYFP+
Sbjct: 167 SGAVGQLVGQFAKLLGCYVVGSAGTKEKVDLLKNKFGFDEAFNYKEEKDLEAALKRYFPN 226

Query: 452 GIDIYFENVGGKMLDAVLVNMRSHGRIAVCGMISQYNLENPEGVHNLSYVITKQLRLQGF 273
           GI+IYFENVGGKMLDAVL NMR H RIAVCGMISQYNLE PEGVHNL  +ITK++R++GF
Sbjct: 227 GINIYFENVGGKMLDAVLANMRLHSRIAVCGMISQYNLERPEGVHNLFCLITKRVRMEGF 286

Query: 272 LVFDYYHQYPKFLEMVLPAIKEGKIKYLEDTAEGLESAPAALIGLFSGRN 123
           +VFDYYH YPKFLEM+LP IK GKI Y+ED AEGLESAPAALIGLFSGRN
Sbjct: 287 IVFDYYHLYPKFLEMILPCIKGGKITYVEDVAEGLESAPAALIGLFSGRN 336



 Score = 97.4 bits (241), Expect(2) = e-132
 Identities = 44/58 (75%), Positives = 48/58 (82%)
 Frame = -3

Query: 1063 LVKNLYLSCDPYMRSRMTKTEGSYVSSFTPNQPIEGYGVAKVLDSGNPKFKKGDLVWG 890
            LVKNLYLSCDPYM  RM K E SYV SFTP  PI G+GVA+VLDSG+P FKKGDL+WG
Sbjct: 48   LVKNLYLSCDPYMCRRMRKLEDSYVESFTPGSPITGFGVARVLDSGHPNFKKGDLIWG 105


>pdb|4HFJ|A Chain A, X-ray Crystal Structure Of A Double Bond Reductase From
           Nicotiana Tabacum gi|444302247|pdb|4HFJ|B Chain B, X-ray
           Crystal Structure Of A Double Bond Reductase From
           Nicotiana Tabacum gi|444302248|pdb|4HFM|A Chain A, X-ray
           Crystal Structure Of A Nadp(h)-bound Double Bond
           Reductase From Nicotiana Tabacum gi|444302249|pdb|4HFM|B
           Chain B, X-ray Crystal Structure Of A Nadp(h)-bound
           Double Bond Reductase From Nicotiana Tabacum
           gi|444302250|pdb|4HFN|A Chain A, X-ray Crystal Structure
           Of A Ternary Complex Of Double Bond Reductase From
           Nicotiana Tabacum gi|444302251|pdb|4HFN|B Chain B, X-ray
           Crystal Structure Of A Ternary Complex Of Double Bond
           Reductase From Nicotiana Tabacum
          Length = 351

 Score =  394 bits (1012), Expect(2) = e-132
 Identities = 188/230 (81%), Positives = 211/230 (91%)
 Frame = -2

Query: 812 TGWEEYSLITNVASLFKIEHTDVPLSYYTGLLGMPGMTAYAGFYEICAPKKGEYVYVSAA 633
           TGWEEYS+IT   +LFKI   DVPLSYYTG+LGMPGMTAYAGF+E+C+PKKGE V+VSAA
Sbjct: 103 TGWEEYSIITPTQTLFKIHDKDVPLSYYTGILGMPGMTAYAGFHEVCSPKKGETVFVSAA 162

Query: 632 SGAVGQLVGQFAKLLGCYVVGSAGSKEKVDLLKNKFGFDEAFNYKEEKDLAAALKRYFPD 453
           SGAVGQLVGQFAK+LGCYVVGSAGSKEKVDLLK+KFGFDEAFNYKEE+DL+AALKRYFPD
Sbjct: 163 SGAVGQLVGQFAKMLGCYVVGSAGSKEKVDLLKSKFGFDEAFNYKEEQDLSAALKRYFPD 222

Query: 452 GIDIYFENVGGKMLDAVLVNMRSHGRIAVCGMISQYNLENPEGVHNLSYVITKQLRLQGF 273
           GIDIYFENVGGKMLDAVLVNM+ +GRIAVCGMISQYNLE  EGVHNL  +ITK++R++GF
Sbjct: 223 GIDIYFENVGGKMLDAVLVNMKLYGRIAVCGMISQYNLEQTEGVHNLFCLITKRIRMEGF 282

Query: 272 LVFDYYHQYPKFLEMVLPAIKEGKIKYLEDTAEGLESAPAALIGLFSGRN 123
           LVFDYYH YPK+LEMV+P IK GK+ Y+ED A GLESAP AL+GLFSGRN
Sbjct: 283 LVFDYYHLYPKYLEMVIPQIKAGKVVYVEDVAHGLESAPTALVGLFSGRN 332



 Score =  103 bits (258), Expect(2) = e-132
 Identities = 47/58 (81%), Positives = 51/58 (87%)
 Frame = -3

Query: 1063 LVKNLYLSCDPYMRSRMTKTEGSYVSSFTPNQPIEGYGVAKVLDSGNPKFKKGDLVWG 890
            +VKNLYLSCDPYMRSRM K EGSYV SF P  PI GYGVAKVL+SG+PKF+KGDLVWG
Sbjct: 44   VVKNLYLSCDPYMRSRMRKIEGSYVESFAPGSPITGYGVAKVLESGDPKFQKGDLVWG 101


>dbj|BAA89423.1| allyl alcohol dehydrogenase [Nicotiana tabacum]
          Length = 343

 Score =  394 bits (1012), Expect(2) = e-132
 Identities = 188/230 (81%), Positives = 211/230 (91%)
 Frame = -2

Query: 812 TGWEEYSLITNVASLFKIEHTDVPLSYYTGLLGMPGMTAYAGFYEICAPKKGEYVYVSAA 633
           TGWEEYS+IT   +LFKI   DVPLSYYTG+LGMPGMTAYAGF+E+C+PKKGE V+VSAA
Sbjct: 103 TGWEEYSIITPTQTLFKIHDKDVPLSYYTGILGMPGMTAYAGFHEVCSPKKGETVFVSAA 162

Query: 632 SGAVGQLVGQFAKLLGCYVVGSAGSKEKVDLLKNKFGFDEAFNYKEEKDLAAALKRYFPD 453
           SGAVGQLVGQFAK+LGCYVVGSAGSKEKVDLLK+KFGFDEAFNYKEE+DL+AALKRYFPD
Sbjct: 163 SGAVGQLVGQFAKMLGCYVVGSAGSKEKVDLLKSKFGFDEAFNYKEEQDLSAALKRYFPD 222

Query: 452 GIDIYFENVGGKMLDAVLVNMRSHGRIAVCGMISQYNLENPEGVHNLSYVITKQLRLQGF 273
           GIDIYFENVGGKMLDAVLVNM+ +GRIAVCGMISQYNLE  EGVHNL  +ITK++R++GF
Sbjct: 223 GIDIYFENVGGKMLDAVLVNMKLYGRIAVCGMISQYNLEQTEGVHNLFCLITKRIRMEGF 282

Query: 272 LVFDYYHQYPKFLEMVLPAIKEGKIKYLEDTAEGLESAPAALIGLFSGRN 123
           LVFDYYH YPK+LEMV+P IK GK+ Y+ED A GLESAP AL+GLFSGRN
Sbjct: 283 LVFDYYHLYPKYLEMVIPQIKAGKVVYVEDVAHGLESAPTALVGLFSGRN 332



 Score =  103 bits (258), Expect(2) = e-132
 Identities = 47/58 (81%), Positives = 51/58 (87%)
 Frame = -3

Query: 1063 LVKNLYLSCDPYMRSRMTKTEGSYVSSFTPNQPIEGYGVAKVLDSGNPKFKKGDLVWG 890
            +VKNLYLSCDPYMRSRM K EGSYV SF P  PI GYGVAKVL+SG+PKF+KGDLVWG
Sbjct: 44   VVKNLYLSCDPYMRSRMRKIEGSYVESFAPGSPITGYGVAKVLESGDPKFQKGDLVWG 101


>ref|XP_002279359.1| PREDICTED: NADP-dependent alkenal double bond reductase P2 [Vitis
           vinifera] gi|147792339|emb|CAN61471.1| hypothetical
           protein VITISV_043825 [Vitis vinifera]
          Length = 345

 Score =  403 bits (1036), Expect(2) = e-131
 Identities = 191/230 (83%), Positives = 213/230 (92%)
 Frame = -2

Query: 812 TGWEEYSLITNVASLFKIEHTDVPLSYYTGLLGMPGMTAYAGFYEICAPKKGEYVYVSAA 633
           TGWEEYSLIT    LFKI+HTDVPLSYYTG+LGM GMTAY+GFYEIC+PKKGEYV++SAA
Sbjct: 105 TGWEEYSLITTDERLFKIQHTDVPLSYYTGILGMAGMTAYSGFYEICSPKKGEYVFISAA 164

Query: 632 SGAVGQLVGQFAKLLGCYVVGSAGSKEKVDLLKNKFGFDEAFNYKEEKDLAAALKRYFPD 453
           SGAVGQLVGQFAKLLGCYVVGSAG+KEKVDLLKNKFGFDEAFNYKEE+DL A LKRYFP+
Sbjct: 165 SGAVGQLVGQFAKLLGCYVVGSAGTKEKVDLLKNKFGFDEAFNYKEEQDLEACLKRYFPE 224

Query: 452 GIDIYFENVGGKMLDAVLVNMRSHGRIAVCGMISQYNLENPEGVHNLSYVITKQLRLQGF 273
           GIDIYFENVGGKMLDAVLVNMR HGRIAVCGMISQYNLE PEGV NL  ++TK++R++GF
Sbjct: 225 GIDIYFENVGGKMLDAVLVNMRLHGRIAVCGMISQYNLEEPEGVRNLFTIVTKRIRMEGF 284

Query: 272 LVFDYYHQYPKFLEMVLPAIKEGKIKYLEDTAEGLESAPAALIGLFSGRN 123
           LVFDYYH YPKFL++++P I+EGKI Y+ED AEGLESAP ALIGL+SGRN
Sbjct: 285 LVFDYYHLYPKFLDLIMPYIREGKIVYVEDIAEGLESAPTALIGLYSGRN 334



 Score = 94.0 bits (232), Expect(2) = e-131
 Identities = 43/58 (74%), Positives = 46/58 (79%)
 Frame = -3

Query: 1063 LVKNLYLSCDPYMRSRMTKTEGSYVSSFTPNQPIEGYGVAKVLDSGNPKFKKGDLVWG 890
            LVKNLYLSCDPYMRSRM   +GSYV  F P  PI GYGVAKVLDS +P F+ GDLVWG
Sbjct: 46   LVKNLYLSCDPYMRSRMRNIQGSYVEPFKPGSPITGYGVAKVLDSRHPDFRTGDLVWG 103


>gb|ACN65116.1| 2-alkenal reductase [Artemisia annua]
          Length = 347

 Score =  385 bits (989), Expect(2) = e-130
 Identities = 182/230 (79%), Positives = 207/230 (90%)
 Frame = -2

Query: 812 TGWEEYSLITNVASLFKIEHTDVPLSYYTGLLGMPGMTAYAGFYEICAPKKGEYVYVSAA 633
           TGWEEYS+I     LFKIEHTDVPLSYYTG+LGMPGMTAY GFYEIC PKKGEYV+VSAA
Sbjct: 107 TGWEEYSIINAPEGLFKIEHTDVPLSYYTGILGMPGMTAYVGFYEICTPKKGEYVFVSAA 166

Query: 632 SGAVGQLVGQFAKLLGCYVVGSAGSKEKVDLLKNKFGFDEAFNYKEEKDLAAALKRYFPD 453
           SGAVGQLVGQFAKL GCYVVGSAG+KEKVDLLKNKFGFDEAFNYKEE+DL AALKRYFP+
Sbjct: 167 SGAVGQLVGQFAKLSGCYVVGSAGTKEKVDLLKNKFGFDEAFNYKEEQDLDAALKRYFPE 226

Query: 452 GIDIYFENVGGKMLDAVLVNMRSHGRIAVCGMISQYNLENPEGVHNLSYVITKQLRLQGF 273
           GIDIYFENVGG+MLDAVL+NMR  GRI+VCGMISQYNLE  EGV NL  ++TK++ ++GF
Sbjct: 227 GIDIYFENVGGRMLDAVLLNMRLDGRISVCGMISQYNLEQSEGVRNLFTLVTKRVTMKGF 286

Query: 272 LVFDYYHQYPKFLEMVLPAIKEGKIKYLEDTAEGLESAPAALIGLFSGRN 123
           +VFD+YH+YPK+LEM++P IK G I Y+ED  EGLE+APAALIGL+SG+N
Sbjct: 287 IVFDHYHKYPKYLEMIIPLIKNGTINYIEDIVEGLENAPAALIGLYSGKN 336



 Score =  107 bits (266), Expect(2) = e-130
 Identities = 48/58 (82%), Positives = 52/58 (89%)
 Frame = -3

Query: 1063 LVKNLYLSCDPYMRSRMTKTEGSYVSSFTPNQPIEGYGVAKVLDSGNPKFKKGDLVWG 890
            LVKNLYLSCDPYMRSRMTKTEGSYV SFTP  P+ GYGVAKVL+SG+  FKKGDL+WG
Sbjct: 48   LVKNLYLSCDPYMRSRMTKTEGSYVESFTPGSPLTGYGVAKVLESGHANFKKGDLIWG 105