BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00011856
         (1137 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AED99874.1| cytochrome P450 [Panax notoginseng]                    449   e-124
gb|ADD84651.1| CYP81B36 [Scoparia dulcis]                             367   2e-99
ref|XP_002285105.1| PREDICTED: isoflavone 2'-hydroxylase-like [V...   367   2e-99
ref|XP_002510130.1| cytochrome P450, putative [Ricinus communis]...   363   3e-98
ref|XP_002283235.1| PREDICTED: isoflavone 2'-hydroxylase [Vitis ...   353   4e-95

>gb|AED99874.1| cytochrome P450 [Panax notoginseng]
          Length = 509

 Score =  449 bits (1155), Expect = e-124
 Identities = 218/328 (66%), Positives = 262/328 (79%)
 Frame = +3

Query: 72   MDIAHRFFMYFPIITVVYVITQHLLRKRLNYPPTPFPILPLIGHLYLLKKSLQETLSNLS 251
            M++ H FF+Y PI   +YV+TQH L K  N+PPTPFP LPLIGHLYLLKK L++TLS LS
Sbjct: 1    MEMEHIFFLYLPIFIALYVLTQHALHKFQNFPPTPFPSLPLIGHLYLLKKPLKQTLSKLS 60

Query: 252  DRYGPVFFLWLGXXXXXXXXXXXAAEECFTKNDITFANRPHLLIGKYFGYNYSSIVWGPY 431
             +YGPVFFLW G           AAEECFTKNDI FANRPHLL+GK+ GYNY+SIVWGPY
Sbjct: 61   HKYGPVFFLWFGSRRVLVISSASAAEECFTKNDIIFANRPHLLVGKHLGYNYTSIVWGPY 120

Query: 432  GDHWRNLRRISTIEILSSHRLQKLSQIRADEVQKLVHNLFQPCIEGKYQPLQMKTVFYKL 611
            GDHWRNLRRIS+IE+LSSHRLQKLS IR DEV+ LVH+ ++  +E   QPL +K +FY L
Sbjct: 121  GDHWRNLRRISSIEVLSSHRLQKLSYIRVDEVKTLVHHFYRAYVEKSNQPLLIKPLFYDL 180

Query: 612  MFNVMTRMVAGKRYFVENTSSISEEAKRFQEIVTEASKLAGASNIGDFMPVVRWFMYNKM 791
            MFNVMTRM+AGK+Y+ ENTS  S EAKRFQEIV E ++LAG SN+ DFMP++  F ++ +
Sbjct: 181  MFNVMTRMIAGKKYYGENTSQ-SAEAKRFQEIVIETARLAGESNLADFMPILXRFXFSGI 239

Query: 792  EKRFQATFKKRDELMQKWIQDFRDKKSDESADPKEKSMIEILLSLQQSEPEYYTDEIIKS 971
             KR+ A +KKRDEL+QKWI +FR+  S+ESA  +EKSMIEILLSL ++EPEYYTDE IKS
Sbjct: 240  VKRYVALYKKRDELVQKWIDEFREMGSNESAGRREKSMIEILLSLHEAEPEYYTDEDIKS 299

Query: 972  LMLALLHAGVSTSIDTMEWAMACLLNNP 1055
            LML+LL AGVSTS+DTMEWA + LLN P
Sbjct: 300  LMLSLLQAGVSTSVDTMEWATSLLLNRP 327


>gb|ADD84651.1| CYP81B36 [Scoparia dulcis]
          Length = 502

 Score =  367 bits (943), Expect = 2e-99
 Identities = 184/347 (53%), Positives = 242/347 (69%)
 Frame = +3

Query: 90   FFMYFPIITVVYVITQHLLRKRLNYPPTPFPILPLIGHLYLLKKSLQETLSNLSDRYGPV 269
            F +Y P+   +Y+IT HLL K  N PP+PFP LP++GHLYLLKK L   L+++S+RYGP+
Sbjct: 4    FLLYIPLAIALYIITNHLLHKISNLPPSPFPSLPILGHLYLLKKPLYRALASVSNRYGPI 63

Query: 270  FFLWLGXXXXXXXXXXXAAEECFTKNDITFANRPHLLIGKYFGYNYSSIVWGPYGDHWRN 449
             FL LG            AEECFTKNDI FANRPHLL GKY GYNY+S+VW  YGDHWRN
Sbjct: 64   VFLNLGSRRVLVVSSPSLAEECFTKNDIVFANRPHLLSGKYVGYNYTSMVWSSYGDHWRN 123

Query: 450  LRRISTIEILSSHRLQKLSQIRADEVQKLVHNLFQPCIEGKYQPLQMKTVFYKLMFNVMT 629
            LR+++++E+LS++RLQ L  IRADEV+ ++  L +   E K  P+ MK V ++L  NV+ 
Sbjct: 124  LRKVASLEVLSTYRLQTLHGIRADEVKSMIKKLNRASDEKK--PVDMKEVCFELTMNVVM 181

Query: 630  RMVAGKRYFVENTSSISEEAKRFQEIVTEASKLAGASNIGDFMPVVRWFMYNKMEKRFQA 809
            RM+AGKRY+ EN   + EEAKRFQEIV E  +L G S +GDF+P+VRW      EK    
Sbjct: 182  RMIAGKRYYGENVEDV-EEAKRFQEIVKETLRLGGTS-MGDFLPIVRWLGIGGAEKGLAE 239

Query: 810  TFKKRDELMQKWIQDFRDKKSDESADPKEKSMIEILLSLQQSEPEYYTDEIIKSLMLALL 989
              +KRD  MQ+ +++ + +  + S D    +MIE+LL LQ+ EPEYYTD++I+SLML LL
Sbjct: 240  LQRKRDGFMQELVEECKRRIRNSSGDSGPMTMIEMLLPLQEKEPEYYTDKLIRSLMLTLL 299

Query: 990  HAGVSTSIDTMEWAMACLLNNPDVLKKAQNEIDNCVPHYRLIDEADM 1130
             AG  T+  TMEW ++ LLNNP V+KKAQ EIDN + H  LIDE+D+
Sbjct: 300  VAGTDTTAGTMEWVVSLLLNNPHVIKKAQVEIDNHIGHGHLIDESDI 346


>ref|XP_002285105.1| PREDICTED: isoflavone 2'-hydroxylase-like [Vitis vinifera]
          Length = 499

 Score =  367 bits (943), Expect = 2e-99
 Identities = 183/347 (52%), Positives = 246/347 (70%)
 Frame = +3

Query: 93   FMYFPIITVVYVITQHLLRKRLNYPPTPFPILPLIGHLYLLKKSLQETLSNLSDRYGPVF 272
            + Y P+  V+YV T H L K  N PP+PFP LPLIGHLYLLKK L  TLS +SDR+GP+ 
Sbjct: 5    YFYIPLFFVLYVFTSHFLHKFRNLPPSPFPTLPLIGHLYLLKKPLHRTLSKISDRHGPIL 64

Query: 273  FLWLGXXXXXXXXXXXAAEECFTKNDITFANRPHLLIGKYFGYNYSSIVWGPYGDHWRNL 452
            FL  G           A+EECFTKND+ FANRP L+ GK+ GYNY+S+ W P+GDHWRNL
Sbjct: 65   FLRFGSRPVLLVSSPSASEECFTKNDVVFANRPRLIAGKHLGYNYTSMSWAPHGDHWRNL 124

Query: 453  RRISTIEILSSHRLQKLSQIRADEVQKLVHNLFQPCIEGKYQPLQMKTVFYKLMFNVMTR 632
            RRIS+ EILSS+RLQ LS IR+DEV+ LV  LF    + + Q ++MK+ F+++  NVM +
Sbjct: 125  RRISSFEILSSNRLQTLSGIRSDEVRSLVRWLF----KNQSQMVEMKSAFFEMTLNVMMK 180

Query: 633  MVAGKRYFVENTSSISEEAKRFQEIVTEASKLAGASNIGDFMPVVRWFMYNKMEKRFQAT 812
            M+ GKRY+ EN   + EEA+ F+E+V+E  +LAGA+N+ DF+P++ W      E+R    
Sbjct: 181  MIGGKRYYGENIGEV-EEARMFREMVSETFQLAGATNMVDFLPILGWLGLKGTERRLIKL 239

Query: 813  FKKRDELMQKWIQDFRDKKSDESADPKEKSMIEILLSLQQSEPEYYTDEIIKSLMLALLH 992
             K+R+  +Q  I++ R K S  + + ++K+MIE+LLSLQ++EPEYYTDEII+ LML++L 
Sbjct: 240  QKRRESFIQNLIEEHRRKGS--NCEGRQKTMIEVLLSLQETEPEYYTDEIIRGLMLSMLT 297

Query: 993  AGVSTSIDTMEWAMACLLNNPDVLKKAQNEIDNCVPHYRLIDEADMA 1133
             G  TS  TMEWA++ LLNNP VLKKA  EID+ + H RLI+E D+A
Sbjct: 298  GGTDTSAGTMEWALSLLLNNPKVLKKAHQEIDDRLGHDRLIEELDLA 344


>ref|XP_002510130.1| cytochrome P450, putative [Ricinus communis]
            gi|223550831|gb|EEF52317.1| cytochrome P450, putative
            [Ricinus communis]
          Length = 500

 Score =  363 bits (933), Expect = 3e-98
 Identities = 186/344 (54%), Positives = 241/344 (70%)
 Frame = +3

Query: 96   MYFPIITVVYVITQHLLRKRLNYPPTPFPILPLIGHLYLLKKSLQETLSNLSDRYGPVFF 275
            ++F +  V+Y++T H   K  N+PP+PFP LPL+GHL+LLKK L  +LS LS+RYGPV  
Sbjct: 4    LHFLLFFVLYILTNHFFNKIRNHPPSPFPTLPLLGHLHLLKKPLHRSLSTLSNRYGPVLL 63

Query: 276  LWLGXXXXXXXXXXXAAEECFTKNDITFANRPHLLIGKYFGYNYSSIVWGPYGDHWRNLR 455
            L  G           AAEEC TK D  FANRP LL GK+ GYNY+S+ W PYGD WRNLR
Sbjct: 64   LQFGYRRVLVVSSPSAAEECLTKCDTVFANRPRLLAGKHIGYNYTSLAWAPYGDLWRNLR 123

Query: 456  RISTIEILSSHRLQKLSQIRADEVQKLVHNLFQPCIEGKYQPLQMKTVFYKLMFNVMTRM 635
            ++S +EILSSHRLQ LS IR DEV+ L+  LF+   + + + + MK+ F++LM NVM RM
Sbjct: 124  KVSALEILSSHRLQLLSSIRRDEVKLLIQRLFRNNKDCR-EKVDMKSAFFELMLNVMMRM 182

Query: 636  VAGKRYFVENTSSISEEAKRFQEIVTEASKLAGASNIGDFMPVVRWFMYNKMEKRFQATF 815
            +AGKRY+ EN   + EEAKRF+EIV E    AG SN+GDF+P+V   +    EKR +   
Sbjct: 183  IAGKRYYGENVEQV-EEAKRFREIVRETFLAAGTSNMGDFLPLVA--VVGGQEKRLKELG 239

Query: 816  KKRDELMQKWIQDFRDKKSDESADPKEKSMIEILLSLQQSEPEYYTDEIIKSLMLALLHA 995
            K+RD  +Q  I + R + +  S++ + K+MIE+LLSLQ+SEPEYYTDE IKSLML LL A
Sbjct: 240  KRRDGFIQDLIDEHRKRMAACSSEERNKTMIEVLLSLQESEPEYYTDETIKSLMLVLLAA 299

Query: 996  GVSTSIDTMEWAMACLLNNPDVLKKAQNEIDNCVPHYRLIDEAD 1127
            G  TS  TMEWAM+ L+NNP+ LKKAQ EID+ + H RLI+E+D
Sbjct: 300  GTDTSAATMEWAMSLLVNNPEALKKAQTEIDSVIGHDRLINESD 343


>ref|XP_002283235.1| PREDICTED: isoflavone 2'-hydroxylase [Vitis vinifera]
          Length = 498

 Score =  353 bits (906), Expect = 4e-95
 Identities = 184/347 (53%), Positives = 242/347 (69%)
 Frame = +3

Query: 93   FMYFPIITVVYVITQHLLRKRLNYPPTPFPILPLIGHLYLLKKSLQETLSNLSDRYGPVF 272
            ++  PI   +YV T H L K  N PP+PFPILPLIGHLYLLKK L   LS +SDR+GP+ 
Sbjct: 5    YLCIPIFLALYVFTWHFLHKLHNLPPSPFPILPLIGHLYLLKKPLHRNLSKISDRHGPIL 64

Query: 273  FLWLGXXXXXXXXXXXAAEECFTKNDITFANRPHLLIGKYFGYNYSSIVWGPYGDHWRNL 452
            FL  G           AAEECFTKNDI FANRP L+ GK+ GYNY++I   PYGDHWRNL
Sbjct: 65   FLRFGYRPVLIVSSHSAAEECFTKNDIIFANRPRLIAGKHLGYNYTAIGTAPYGDHWRNL 124

Query: 453  RRISTIEILSSHRLQKLSQIRADEVQKLVHNLFQPCIEGKYQPLQMKTVFYKLMFNVMTR 632
            RRIS+ EILSS+RLQ LS IR+DEV+ L+  L    ++ + Q ++MK+ F++L  NV+ R
Sbjct: 125  RRISSFEILSSNRLQMLSGIRSDEVRTLICRL----VKNQNQVVEMKSAFFELTLNVIMR 180

Query: 633  MVAGKRYFVENTSSISEEAKRFQEIVTEASKLAGASNIGDFMPVVRWFMYNKMEKRFQAT 812
            M+ GKRY+ EN   + EEA++F+E+V++A +LAG +N+ DF+P++ W      EKR    
Sbjct: 181  MIGGKRYYGENVGEV-EEARKFREMVSKAFRLAG-TNMVDFLPILGWLGLKGTEKRLMEL 238

Query: 813  FKKRDELMQKWIQDFRDKKSDESADPKEKSMIEILLSLQQSEPEYYTDEIIKSLMLALLH 992
             K RD  +Q  I++ R K S    + + K+MIE+LLSLQ++EPE YTDEII  LML++L 
Sbjct: 239  QKMRDSFIQNLIEEHRIKGS--KCERRPKTMIEVLLSLQETEPENYTDEIIGGLMLSMLT 296

Query: 993  AGVSTSIDTMEWAMACLLNNPDVLKKAQNEIDNCVPHYRLIDEADMA 1133
            AG  TS  TMEWA++ LLN+P+VLKKAQ EID  + H RLI+E D+A
Sbjct: 297  AGTDTSAGTMEWALSLLLNSPEVLKKAQQEIDVHLGHDRLIEEVDLA 343