BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00011693
         (730 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ABC69422.1| CYP72A58 [Nicotiana tabacum]                           229   4e-58
dbj|BAF98466.1| cytochrome P450 [Coptis japonica var. dissecta]       228   8e-58
ref|XP_002265455.1| PREDICTED: secologanin synthase isoform 1 [V...   226   4e-57
ref|NP_188081.1| cytochrome P450, family 72, subfamily A, polype...   224   1e-56
dbj|BAB02395.1| cytochrome P450-like protein [Arabidopsis thaliana]   224   1e-56

>gb|ABC69422.1| CYP72A58 [Nicotiana tabacum]
          Length = 513

 Score =  229 bits (584), Expect = 4e-58
 Identities = 115/233 (49%), Positives = 161/233 (69%), Gaps = 29/233 (12%)
 Frame = +3

Query: 117 GFRYIPIKANRRMNTVCNEIQVLLRGIISKQQ*AIQVGEVQTDDLLGLLLKSNSSEIKEK 296
           G R++P K NRRM  +  +++  ++GII K+  A++ G+   +DLLG+LL+SN  EI++ 
Sbjct: 242 GRRFLPTKRNRRMKEIKKDVRASIKGIIDKRLKAMKAGDTNNEDLLGILLESNIKEIEQH 301

Query: 297 G---FGMTIEEVMKECKLFYFAGSETTSNLQVWTLIMLSTHPEWQIRAREEVVQAFGSKI 467
           G   FGM+IEEV++ECKLFYFAG ETTS L +W+L++LS + +WQ RAREE++Q FGS+ 
Sbjct: 302 GNKDFGMSIEEVIEECKLFYFAGQETTSVLLLWSLVLLSRYQDWQARAREEILQVFGSRK 361

Query: 468 PDFDGPINHKGC-------------------KICN-------INLPARIIITFPVILLHQ 569
           PDFDG +NH                      + CN       ++LPA ++++ P+ILLH 
Sbjct: 362 PDFDG-LNHLKIVTMILYESLRLYPSLITLTRRCNEDIVLGELSLPAGVLVSLPLILLHH 420

Query: 570 DPEVWGQNSLEFRPERFSEEISSATKGKFSYIPFGRGPRICIGQNFAMIEAKM 728
           D E+WG+++ EF+PERF E ISSATKG+ +Y PF  GPRICIGQNFAM+EAKM
Sbjct: 421 DEEIWGEDAKEFKPERFREGISSATKGQLTYFPFSWGPRICIGQNFAMLEAKM 473


>dbj|BAF98466.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 518

 Score =  228 bits (582), Expect = 8e-58
 Identities = 115/234 (49%), Positives = 154/234 (65%), Gaps = 30/234 (12%)
 Frame = +3

Query: 117 GFRYIPIKANRRMNTVCNEIQVLLRGIISKQQ*AIQVGEVQTDDLLGLLLKSNSSEIKE- 293
           G R++P K N RM  +  E +VLLR I++K++ A+++GE   DDLLGLL++SN  EI + 
Sbjct: 246 GLRFLPTKRNNRMKEIYGEARVLLRDIVNKREKAMKIGEAHNDDLLGLLMESNFKEITDN 305

Query: 294 ---KGFGMTIEEVMKECKLFYFAGSETTSNLQVWTLIMLSTHPEWQIRAREEVVQAFGSK 464
              K FGMTI+EV+ ECKLFYFAG ETTS L VWT+++LS HP+WQ +AREEV+Q FG K
Sbjct: 306 VNSKNFGMTIDEVIDECKLFYFAGQETTSTLLVWTMVVLSMHPDWQEKAREEVLQVFGGK 365

Query: 465 IPDFDGPINH--------------------------KGCKICNINLPARIIITFPVILLH 566
            PDFDG +NH                          K  K+  I LPA + +  P +L+H
Sbjct: 366 DPDFDG-LNHLKIVTMILYEVLRLYPPAVLMARGTYKTMKLGEITLPAGVHLAMPTLLVH 424

Query: 567 QDPEVWGQNSLEFRPERFSEEISSATKGKFSYIPFGRGPRICIGQNFAMIEAKM 728
            D E+WG+++ +F PERFS  +S ATK + S+ PF  GPRICIGQNFA++E+KM
Sbjct: 425 HDRELWGEDAEDFNPERFSGGVSKATKNQVSFFPFSWGPRICIGQNFALLESKM 478


>ref|XP_002265455.1| PREDICTED: secologanin synthase isoform 1 [Vitis vinifera]
          Length = 515

 Score =  226 bits (576), Expect = 4e-57
 Identities = 111/234 (47%), Positives = 157/234 (67%), Gaps = 30/234 (12%)
 Frame = +3

Query: 117 GFRYIPIKANRRMNTVCNEIQVLLRGIISKQQ*AIQVGEVQTDDLLGLLLKSNSSEIKEK 296
           G+R++P K N+RM  + NE+  LL+GII +++ A++VGE   DDLLGLL++SN  E++E 
Sbjct: 243 GWRFVPTKTNKRMRQISNEVNALLKGIIERREKAMKVGETANDDLLGLLMESNYKEMQEH 302

Query: 297 G----FGMTIEEVMKECKLFYFAGSETTSNLQVWTLIMLSTHPEWQIRAREEVVQAFGSK 464
           G     GM+ ++V++ECKLFYFAG ETTS L +WT+++LS H  WQ RAREEV+Q FG+K
Sbjct: 303 GERKNVGMSNKDVIEECKLFYFAGQETTSVLLLWTMVLLSKHSNWQARAREEVLQVFGNK 362

Query: 465 IPDFDGPINH--------------------------KGCKICNINLPARIIITFPVILLH 566
            PD DG +NH                             ++  + LP  + ++ P++LLH
Sbjct: 363 KPDGDG-LNHLKIVTMIFHEVLRLYPPASMLIRSVYADTEVGGMYLPDGVQVSLPILLLH 421

Query: 567 QDPEVWGQNSLEFRPERFSEEISSATKGKFSYIPFGRGPRICIGQNFAMIEAKM 728
            D E+WG ++ +F PERFSE +S ATKG+F++ PFG GPR+CIGQNFAM+EAKM
Sbjct: 422 HDHEIWGDDAKDFNPERFSEGVSKATKGQFAFFPFGYGPRVCIGQNFAMMEAKM 475


>ref|NP_188081.1| cytochrome P450, family 72, subfamily A, polypeptide 9 [Arabidopsis
           thaliana] gi|332642028|gb|AEE75549.1| cytochrome P450,
           family 72, subfamily A, polypeptide 9 [Arabidopsis
           thaliana]
          Length = 508

 Score =  224 bits (572), Expect = 1e-56
 Identities = 113/228 (49%), Positives = 151/228 (66%), Gaps = 25/228 (10%)
 Frame = +3

Query: 120 FRYIPIKANRRMNTVCNEIQVLLRGIISKQQ*AIQVGEVQTDDLLGLLLKSNSSEIKEKG 299
           +R+ P K NRRM T+  EIQV+LRGIIS ++ A   GE  +DDLLG+LLKSNS +   KG
Sbjct: 243 YRHFPTKNNRRMKTIVKEIQVILRGIISHREKARDAGEAPSDDLLGILLKSNSEQ--SKG 300

Query: 300 FGMTIEEVMKECKLFYFAGSETTSNLQVWTLIMLSTHPEWQIRAREEVVQAFGSKIPDFD 479
            G+ +EE+M+ECKLFYFAG ETTS L  WT+++LS H +WQ RAREEV+Q FG   PD  
Sbjct: 301 NGLNMEEIMEECKLFYFAGQETTSVLLAWTMVLLSQHQDWQARAREEVMQVFGHNKPDLQ 360

Query: 480 G-------------------PI------NHKGCKICNINLPARIIITFPVILLHQDPEVW 584
           G                   P+       HK  K+ ++ LP  I +  PV+L+H+D ++W
Sbjct: 361 GINQLKVMTMIIYEVLRLYPPVIQMNRATHKEIKLGDMTLPGGIQVHMPVLLIHRDTKLW 420

Query: 585 GQNSLEFRPERFSEEISSATKGKFSYIPFGRGPRICIGQNFAMIEAKM 728
           G ++ EF+PERF + I+ ATK +  ++PFG GPRICIGQNFA++EAKM
Sbjct: 421 GDDAAEFKPERFKDGIAKATKNQVCFLPFGWGPRICIGQNFALLEAKM 468


>dbj|BAB02395.1| cytochrome P450-like protein [Arabidopsis thaliana]
          Length = 506

 Score =  224 bits (572), Expect = 1e-56
 Identities = 113/228 (49%), Positives = 151/228 (66%), Gaps = 25/228 (10%)
 Frame = +3

Query: 120 FRYIPIKANRRMNTVCNEIQVLLRGIISKQQ*AIQVGEVQTDDLLGLLLKSNSSEIKEKG 299
           +R+ P K NRRM T+  EIQV+LRGIIS ++ A   GE  +DDLLG+LLKSNS +   KG
Sbjct: 241 YRHFPTKNNRRMKTIVKEIQVILRGIISHREKARDAGEAPSDDLLGILLKSNSEQ--SKG 298

Query: 300 FGMTIEEVMKECKLFYFAGSETTSNLQVWTLIMLSTHPEWQIRAREEVVQAFGSKIPDFD 479
            G+ +EE+M+ECKLFYFAG ETTS L  WT+++LS H +WQ RAREEV+Q FG   PD  
Sbjct: 299 NGLNMEEIMEECKLFYFAGQETTSVLLAWTMVLLSQHQDWQARAREEVMQVFGHNKPDLQ 358

Query: 480 G-------------------PI------NHKGCKICNINLPARIIITFPVILLHQDPEVW 584
           G                   P+       HK  K+ ++ LP  I +  PV+L+H+D ++W
Sbjct: 359 GINQLKVMTMIIYEVLRLYPPVIQMNRATHKEIKLGDMTLPGGIQVHMPVLLIHRDTKLW 418

Query: 585 GQNSLEFRPERFSEEISSATKGKFSYIPFGRGPRICIGQNFAMIEAKM 728
           G ++ EF+PERF + I+ ATK +  ++PFG GPRICIGQNFA++EAKM
Sbjct: 419 GDDAAEFKPERFKDGIAKATKNQVCFLPFGWGPRICIGQNFALLEAKM 466