BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00011581
         (1072 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI16383.3| unnamed protein product [Vitis vinifera]              260   4e-67
ref|XP_002282827.1| PREDICTED: tRNA-specific 2-thiouridylase mnm...   260   4e-67
ref|XP_004142116.1| PREDICTED: tRNA-specific 2-thiouridylase Mnm...   235   2e-59
ref|XP_002298404.1| predicted protein [Populus trichocarpa] gi|2...   220   4e-55
ref|XP_002513510.1| tRNA (5-methylaminomethyl-2-thiouridylate)-m...   217   5e-54

>emb|CBI16383.3| unnamed protein product [Vitis vinifera]
          Length = 544

 Score =  260 bits (665), Expect = 4e-67
 Identities = 126/201 (62%), Positives = 162/201 (80%), Gaps = 3/201 (1%)
 Frame = +1

Query: 151 YVVEKDTKHNVVYVSRSYFSVDKRRRSFRVGSLKWLCEKHPNEINHLRCKVRHGPGFYDC 330
           YVVEKD K+NVV+VSR+YFS+DKRRR FRVGSLKW+  + P +IN L+CKVRHGPGFY+C
Sbjct: 344 YVVEKDVKNNVVFVSRNYFSLDKRRRLFRVGSLKWISREPPGQINQLQCKVRHGPGFYNC 403

Query: 331 SLTMEPGENTHDKVAVVQLSEDDQGLAAGQFAAFYDEKTCLGSGVILESWDDSGFPICPK 510
           S TMEPGE+ H+ VAVVQLSEDDQGLAAGQF AFY  ++C+GSGVILESWDD GFP+C K
Sbjct: 404 SFTMEPGEDGHEDVAVVQLSEDDQGLAAGQFTAFYQGRSCIGSGVILESWDDQGFPVCAK 463

Query: 511 ALEIAKMEDKSKLGKPVKIKVKPSTISDTNE--DSREANQYMVDSKSAISAK-RRPVEHQ 681
           ALEIA+MEDKSKLGKPVKIKV+P T    ++  D  + ++ + +S++A++ +  R  E +
Sbjct: 464 ALEIARMEDKSKLGKPVKIKVRPETPPKGSDQIDGIKLDRELANSQAAVTDEISRSSEGE 523

Query: 682 KKIRTFPFHWLQQFTKKWMQI 744
            + R FP +WLQ+  +KW+Q+
Sbjct: 524 ARSR-FPMNWLQKIREKWLQM 543


>ref|XP_002282827.1| PREDICTED: tRNA-specific 2-thiouridylase mnmA-like [Vitis vinifera]
          Length = 540

 Score =  260 bits (665), Expect = 4e-67
 Identities = 126/201 (62%), Positives = 162/201 (80%), Gaps = 3/201 (1%)
 Frame = +1

Query: 151 YVVEKDTKHNVVYVSRSYFSVDKRRRSFRVGSLKWLCEKHPNEINHLRCKVRHGPGFYDC 330
           YVVEKD K+NVV+VSR+YFS+DKRRR FRVGSLKW+  + P +IN L+CKVRHGPGFY+C
Sbjct: 340 YVVEKDVKNNVVFVSRNYFSLDKRRRLFRVGSLKWISREPPGQINQLQCKVRHGPGFYNC 399

Query: 331 SLTMEPGENTHDKVAVVQLSEDDQGLAAGQFAAFYDEKTCLGSGVILESWDDSGFPICPK 510
           S TMEPGE+ H+ VAVVQLSEDDQGLAAGQF AFY  ++C+GSGVILESWDD GFP+C K
Sbjct: 400 SFTMEPGEDGHEDVAVVQLSEDDQGLAAGQFTAFYQGRSCIGSGVILESWDDQGFPVCAK 459

Query: 511 ALEIAKMEDKSKLGKPVKIKVKPSTISDTNE--DSREANQYMVDSKSAISAK-RRPVEHQ 681
           ALEIA+MEDKSKLGKPVKIKV+P T    ++  D  + ++ + +S++A++ +  R  E +
Sbjct: 460 ALEIARMEDKSKLGKPVKIKVRPETPPKGSDQIDGIKLDRELANSQAAVTDEISRSSEGE 519

Query: 682 KKIRTFPFHWLQQFTKKWMQI 744
            + R FP +WLQ+  +KW+Q+
Sbjct: 520 ARSR-FPMNWLQKIREKWLQM 539


>ref|XP_004142116.1| PREDICTED: tRNA-specific 2-thiouridylase MnmA-like [Cucumis
           sativus] gi|449524848|ref|XP_004169433.1| PREDICTED:
           tRNA-specific 2-thiouridylase MnmA-like [Cucumis
           sativus]
          Length = 538

 Score =  235 bits (599), Expect = 2e-59
 Identities = 117/201 (58%), Positives = 145/201 (72%), Gaps = 2/201 (0%)
 Frame = +1

Query: 151 YVVEKDTKHNVVYVSRSYFSVDKRRRSFRVGSLKWLCEKHPNEINHLRCKVRHGPGFYDC 330
           YVVEKD K+NVV+VSR+YFS DKRRR FRVGSLKWL    P++   L+CKVRHGP FY C
Sbjct: 338 YVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLSGFPPDQFTELQCKVRHGPSFYSC 397

Query: 331 SLTMEPGENTHDKVAVVQLSEDDQGLAAGQFAAFYDEKTCLGSGVILESWDDSGFPICPK 510
           +LTME  E+ H+ VAVV LSEDDQGLAAGQFAAFY  ++C+GSGVILESWDD GFP+C K
Sbjct: 398 NLTMEHSEDGHEDVAVVHLSEDDQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQK 457

Query: 511 ALEIAKMEDKSKLGKPVKIKVKPST--ISDTNEDSREANQYMVDSKSAISAKRRPVEHQK 684
           A+E A+MEDKSKLGKP+KIKVKP +       +D  E +  + + ++ +  + R V   +
Sbjct: 458 AIENAQMEDKSKLGKPIKIKVKPESGMKESKAKDDTEIHDKLRNEEATVVEQIREVSPDE 517

Query: 685 KIRTFPFHWLQQFTKKWMQIF 747
            + T    WL    KK +QIF
Sbjct: 518 AMATPSMKWLGSLKKKLLQIF 538


>ref|XP_002298404.1| predicted protein [Populus trichocarpa] gi|222845662|gb|EEE83209.1|
           predicted protein [Populus trichocarpa]
          Length = 434

 Score =  220 bits (561), Expect = 4e-55
 Identities = 106/170 (62%), Positives = 132/170 (77%), Gaps = 2/170 (1%)
 Frame = +1

Query: 151 YVVEKDTKHNVVYVSRSYFSVDKRRRSFRVGSLKWLCEKHPNEINHLRCKVRHGPGFYDC 330
           YVVEKD K+NVV+VSR+YFS+DKRRRSFRVGSLKWL     +    L+CKVRHGP FYDC
Sbjct: 264 YVVEKDVKNNVVFVSRNYFSLDKRRRSFRVGSLKWLSGSPTDMTTQLQCKVRHGPTFYDC 323

Query: 331 SLTMEPGENTHDKVAVVQLSEDDQGLAAGQFAAFYDEKTCLGSGVILESWDDSGFPICPK 510
           S+ +E GE++ ++V  V L EDDQGLAAGQF AFY  +TC+GSGVILESWDD GFP+C K
Sbjct: 324 SVKIELGEDSPEEVVAVHLCEDDQGLAAGQFTAFYQGRTCIGSGVILESWDDKGFPVCQK 383

Query: 511 ALEIAKMEDKSKLGKPVKIKVKPSTI--SDTNEDSREANQYMVDSKSAIS 654
           ALE+A++EDKSKLGKPVKIKVKP T       +D  E ++ + + + A++
Sbjct: 384 ALELARLEDKSKLGKPVKIKVKPETSDQESVQKDGIEPDKGLAEPRVAVT 433


>ref|XP_002513510.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase,
           putative [Ricinus communis] gi|223547418|gb|EEF48913.1|
           tRNA
           (5-methylaminomethyl-2-thiouridylate)-methyltransferase,
           putative [Ricinus communis]
          Length = 497

 Score =  217 bits (552), Expect = 5e-54
 Identities = 101/143 (70%), Positives = 120/143 (83%)
 Frame = +1

Query: 151 YVVEKDTKHNVVYVSRSYFSVDKRRRSFRVGSLKWLCEKHPNEINHLRCKVRHGPGFYDC 330
           YVVEKD K+NVV+VSR+YFS DKRRR  RVGSL+W     P E + L+CKVRHGPGFY+C
Sbjct: 327 YVVEKDVKNNVVFVSRNYFSFDKRRRLLRVGSLRWFNGSPPKETSELQCKVRHGPGFYNC 386

Query: 331 SLTMEPGENTHDKVAVVQLSEDDQGLAAGQFAAFYDEKTCLGSGVILESWDDSGFPICPK 510
           S+ +EPGE     VA+VQL EDDQGLAAGQFAAFY  +TC+GSGVILESW+D GFP+C +
Sbjct: 387 SVVIEPGEVDDQDVAIVQLCEDDQGLAAGQFAAFYKGRTCIGSGVILESWNDQGFPVCER 446

Query: 511 ALEIAKMEDKSKLGKPVKIKVKP 579
           ALE+A++EDKS+LGKPVKIKVKP
Sbjct: 447 ALELARIEDKSELGKPVKIKVKP 469