BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00011540
         (1731 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI32840.3| unnamed protein product [Vitis vinifera]              466   e-151
ref|XP_002278394.1| PREDICTED: uncharacterized protein LOC100246...   459   e-149
ref|XP_002319188.1| predicted protein [Populus trichocarpa] gi|2...   433   e-138
ref|XP_002525693.1| conserved hypothetical protein [Ricinus comm...   431   e-136
ref|XP_003624694.1| hypothetical protein MTR_7g086460 [Medicago ...   414   e-135

>emb|CBI32840.3| unnamed protein product [Vitis vinifera]
          Length = 502

 Score =  466 bits (1200), Expect(2) = e-151
 Identities = 231/371 (62%), Positives = 278/371 (74%), Gaps = 2/371 (0%)
 Frame = +3

Query: 192  SSLKGVDVENPIINFTPSLLVG--PSQGSKDAVACERIQVSGKSRLKLESYANTYRISLV 365
            SS KGV + NP ++  PS L G   S+GSKD + CER+QV+G SRLKL  YA+++R+++ 
Sbjct: 25   SSFKGVGLGNPTLDVIPSTLPGHLSSRGSKDVLLCERVQVAGLSRLKLGHYASSFRVTVA 84

Query: 366  PSVVIPERLHSKIQICFHRNASLGSCQCQSDDWKHVHNGLWNSVMSPYEDRYLDVKYSGE 545
            PSVVIPERLHSKI +CFH NASLGSCQC+ D+WK    GLWN VMSPYEDRY+DVK+ GE
Sbjct: 85   PSVVIPERLHSKILVCFHGNASLGSCQCEKDEWKTSQKGLWNFVMSPYEDRYVDVKFIGE 144

Query: 546  VPGSVTVTIEEDFQQWRIFCLVLGFMLQLLAPIVSSWVPFYYSSSMAIGVFLVVIILLFQ 725
            + GSVTV++EEDFQ+WR+FCL LGF L LLAP+VSSWVPFYYSSSMAIG+ LV+IILLFQ
Sbjct: 145  ISGSVTVSVEEDFQRWRLFCLALGFFLLLLAPVVSSWVPFYYSSSMAIGILLVIIILLFQ 204

Query: 726  GMKLLPTGRKNFFYISIYTSFFGAGSYLLHQFSVMINSILLNFGFSEEMHNPVSVLVFLL 905
            GMKLLPTGRKNFFY++IY S  GAGS+LLHQFS+++N+IL+NFG SEEMHNPVS+   + 
Sbjct: 205  GMKLLPTGRKNFFYLTIYGSVLGAGSFLLHQFSMLVNTILVNFGLSEEMHNPVSIFALVG 264

Query: 906  IVLAGAALGYWLVRKFVIXXXXXXXXXXAQFVKWAMRIIGFTCIFQSTLDTPLALAAQCC 1085
            ++LAGAALGYW+VRKFVI          AQFVKW MR+I  T I QSTLDTPLAL A   
Sbjct: 265  LILAGAALGYWIVRKFVISEDGSVDVGVAQFVKWGMRLIATTFILQSTLDTPLALGALVS 324

Query: 1086 CLMICFTFSRFKWHSLEHLSTHGNRNHQGPTRGRATVKHTRAEFLSRPGTTSRRGNIWNS 1265
            C +I    +  KW    +     NR+  G     AT KH RAEFLSR G T  RG  W+S
Sbjct: 325  CWVIWILITSPKWRRRVYKKYSENRSPWGWKSREATAKHNRAEFLSRSGKTIPRGTPWDS 384

Query: 1266 PNSTKSSAKWT 1298
            P   KSS+ W+
Sbjct: 385  P---KSSSAWS 392



 Score = 98.2 bits (243), Expect(2) = e-151
 Identities = 45/86 (52%), Positives = 61/86 (70%)
 Frame = +1

Query: 1405 TFHKTPNRKKFTKQEWEDFTEESTRQALADWASTPEFTDWIVKNADRVKVVPEEXXXXXX 1584
            TFHKTP+RKKF+K++WEDFT ESTRQA+ D  S+PEFTDWIV++ADR++V+P++      
Sbjct: 417  TFHKTPSRKKFSKKDWEDFTRESTRQAMGDMVSSPEFTDWIVEHADRIQVLPDDSSDETI 476

Query: 1585 XXXXXXXXEAAVENSNNGNVFYKWQQ 1662
                    E AV + N   +F KWQ+
Sbjct: 477  GSGSDSTEETAVLSGNRPGLF-KWQR 501


>ref|XP_002278394.1| PREDICTED: uncharacterized protein LOC100246460 [Vitis vinifera]
          Length = 537

 Score =  459 bits (1181), Expect(2) = e-149
 Identities = 227/366 (62%), Positives = 274/366 (74%), Gaps = 2/366 (0%)
 Frame = +3

Query: 207  VDVENPIINFTPSLLVG--PSQGSKDAVACERIQVSGKSRLKLESYANTYRISLVPSVVI 380
            V + NP ++  PS L G   S+GSKD + CER+QV+G SRLKL  YA+++R+++ PSVVI
Sbjct: 65   VGLGNPTLDVIPSTLPGHLSSRGSKDVLLCERVQVAGLSRLKLGHYASSFRVTVAPSVVI 124

Query: 381  PERLHSKIQICFHRNASLGSCQCQSDDWKHVHNGLWNSVMSPYEDRYLDVKYSGEVPGSV 560
            PERLHSKI +CFH NASLGSCQC+ D+WK    GLWN VMSPYEDRY+DVK+ GE+ GSV
Sbjct: 125  PERLHSKILVCFHGNASLGSCQCEKDEWKTSQKGLWNFVMSPYEDRYVDVKFIGEISGSV 184

Query: 561  TVTIEEDFQQWRIFCLVLGFMLQLLAPIVSSWVPFYYSSSMAIGVFLVVIILLFQGMKLL 740
            TV++EEDFQ+WR+FCL LGF L LLAP+VSSWVPFYYSSSMAIG+ LV+IILLFQGMKLL
Sbjct: 185  TVSVEEDFQRWRLFCLALGFFLLLLAPVVSSWVPFYYSSSMAIGILLVIIILLFQGMKLL 244

Query: 741  PTGRKNFFYISIYTSFFGAGSYLLHQFSVMINSILLNFGFSEEMHNPVSVLVFLLIVLAG 920
            PTGRKNFFY++IY S  GAGS+LLHQFS+++N+IL+NFG SEEMHNPVS+   + ++LAG
Sbjct: 245  PTGRKNFFYLTIYGSVLGAGSFLLHQFSMLVNTILVNFGLSEEMHNPVSIFALVGLILAG 304

Query: 921  AALGYWLVRKFVIXXXXXXXXXXAQFVKWAMRIIGFTCIFQSTLDTPLALAAQCCCLMIC 1100
            AALGYW+VRKFVI          AQFVKW MR+I  T I QSTLDTPLAL A   C +I 
Sbjct: 305  AALGYWIVRKFVISEDGSVDVGVAQFVKWGMRLIATTFILQSTLDTPLALGALVSCWVIW 364

Query: 1101 FTFSRFKWHSLEHLSTHGNRNHQGPTRGRATVKHTRAEFLSRPGTTSRRGNIWNSPNSTK 1280
               +  KW    +     NR+  G     AT KH RAEFLSR G T  RG  W+SP   K
Sbjct: 365  ILITSPKWRRRVYKKYSENRSPWGWKSREATAKHNRAEFLSRSGKTIPRGTPWDSP---K 421

Query: 1281 SSAKWT 1298
            SS+ W+
Sbjct: 422  SSSAWS 427



 Score = 98.2 bits (243), Expect(2) = e-149
 Identities = 45/86 (52%), Positives = 61/86 (70%)
 Frame = +1

Query: 1405 TFHKTPNRKKFTKQEWEDFTEESTRQALADWASTPEFTDWIVKNADRVKVVPEEXXXXXX 1584
            TFHKTP+RKKF+K++WEDFT ESTRQA+ D  S+PEFTDWIV++ADR++V+P++      
Sbjct: 452  TFHKTPSRKKFSKKDWEDFTRESTRQAMGDMVSSPEFTDWIVEHADRIQVLPDDSSDETI 511

Query: 1585 XXXXXXXXEAAVENSNNGNVFYKWQQ 1662
                    E AV + N   +F KWQ+
Sbjct: 512  GSGSDSTEETAVLSGNRPGLF-KWQR 536


>ref|XP_002319188.1| predicted protein [Populus trichocarpa] gi|222857564|gb|EEE95111.1|
            predicted protein [Populus trichocarpa]
          Length = 501

 Score =  433 bits (1113), Expect(2) = e-138
 Identities = 217/372 (58%), Positives = 266/372 (71%), Gaps = 3/372 (0%)
 Frame = +3

Query: 192  SSLKGVDVENPIINFTPSLL---VGPSQGSKDAVACERIQVSGKSRLKLESYANTYRISL 362
            ++LKG+D ENP ++ TPS     V    GSKDA  CER++VSG  R KL  YA+++R++L
Sbjct: 31   TTLKGIDSENPAVDVTPSHFHERVFSHDGSKDAFFCERVKVSGHLRWKLSRYASSFRVTL 90

Query: 363  VPSVVIPERLHSKIQICFHRNASLGSCQCQSDDWKHVHNGLWNSVMSPYEDRYLDVKYSG 542
             PS +IPERLH  IQ+CFHRNASLG CQC+ DDW+ V  GLW SVMSPYE+RY+DVK+ G
Sbjct: 91   APSALIPERLHINIQVCFHRNASLGLCQCEKDDWRIVQKGLWTSVMSPYEERYVDVKFIG 150

Query: 543  EVPGSVTVTIEEDFQQWRIFCLVLGFMLQLLAPIVSSWVPFYYSSSMAIGVFLVVIILLF 722
            +  GSV++ ++ED QQWR+ CL +GF+L LLAPIVSSWVPFYYS+SMAIGVFLV+IILLF
Sbjct: 151  DTSGSVSIAVDEDLQQWRLMCLAVGFVLLLLAPIVSSWVPFYYSTSMAIGVFLVIIILLF 210

Query: 723  QGMKLLPTGRKNFFYISIYTSFFGAGSYLLHQFSVMINSILLNFGFSEEMHNPVSVLVFL 902
            QGMKLLPTGRKNFFY+SIY S  GAG+++LHQ S ++NSIL+NFG SE+MHNPV + + +
Sbjct: 211  QGMKLLPTGRKNFFYLSIYGSVLGAGTFILHQISTLVNSILVNFGLSEDMHNPVYIFILV 270

Query: 903  LIVLAGAALGYWLVRKFVIXXXXXXXXXXAQFVKWAMRIIGFTCIFQSTLDTPLALAAQC 1082
             IVL GA LG+W+VRKFVI          AQFVKWAMRII  T I QSTLDTPLA+ A  
Sbjct: 271  GIVLTGAGLGFWMVRKFVISKDGSVDDGVAQFVKWAMRIIASTFILQSTLDTPLAMGALL 330

Query: 1083 CCLMICFTFSRFKWHSLEHLSTHGNRNHQGPTRGRATVKHTRAEFLSRPGTTSRRGNIWN 1262
                IC      KW      S  G  +      G+ T +  RAEF SR G  S +G +WN
Sbjct: 331  SSCAICSV--TLKWDQ----SDSGGGSAWLQPAGQTTARFRRAEFHSRSGKMSPQGKMWN 384

Query: 1263 SPNSTKSSAKWT 1298
            SP   KSS+ WT
Sbjct: 385  SP---KSSSAWT 393



 Score = 88.6 bits (218), Expect(2) = e-138
 Identities = 37/84 (44%), Positives = 53/84 (63%)
 Frame = +1

Query: 1405 TFHKTPNRKKFTKQEWEDFTEESTRQALADWASTPEFTDWIVKNADRVKVVPEEXXXXXX 1584
            TFHKTP RKKFTK++WEDFT EST +A+ +WA++PE T+WI+ NADR++++P        
Sbjct: 418  TFHKTPRRKKFTKKQWEDFTRESTHEAVMEWAASPEVTNWIINNADRIQLLPSNYGSEEM 477

Query: 1585 XXXXXXXXEAAVENSNNGNVFYKW 1656
                    +A+V  S      + W
Sbjct: 478  VGSESDSTDASVAGSGKPFSLFNW 501


>ref|XP_002525693.1| conserved hypothetical protein [Ricinus communis]
            gi|223534993|gb|EEF36676.1| conserved hypothetical
            protein [Ricinus communis]
          Length = 502

 Score =  431 bits (1107), Expect(2) = e-136
 Identities = 214/377 (56%), Positives = 273/377 (72%), Gaps = 11/377 (2%)
 Frame = +3

Query: 201  KGVDVENPIINFTPSLLVG--PSQGSKDAVACERIQVSGKSRLKLESYANTYRISLVPSV 374
            KG+D+ NP+I+ TPS L G  P++ S+D + CER++VSG SR++  SYAN++R++L PS 
Sbjct: 39   KGIDLGNPVIDITPSFLNGKFPNRVSRDVLYCERVKVSGHSRIEWGSYANSFRVTLAPSA 98

Query: 375  VIPERLHSKIQICFHRNASLGSCQCQSDDWKHVHNGLWNSVMSPYEDRYLDVKYSGEVPG 554
            VIPERLHSKIQ+CFHRN+SL  C C+ D+W+ V  G+W+SVMSPY+++Y+DVK+ GEV G
Sbjct: 99   VIPERLHSKIQVCFHRNSSLALCHCEKDEWRTVQKGMWSSVMSPYDEKYVDVKFIGEVAG 158

Query: 555  SVTVTIEEDFQQWRIFCLVLGFMLQLLAPIVSSWVPFYYSSSMAIGVFLVVIILLFQGMK 734
             V +++EE FQQWR+ CL +GF+L LLAPI+SSWVPFYY++SMAIGVFLVVIILLFQGMK
Sbjct: 159  PVKISVEEVFQQWRLLCLAVGFLLLLLAPIISSWVPFYYTTSMAIGVFLVVIILLFQGMK 218

Query: 735  LLPTGRKNFFYISIYTSFFGAGSYLLHQFSVMINSILLNFGFSEEMHNPVSVLVFLLIVL 914
            LLPTGRKN FY+SIY S  GAG+++LH  S+++NSIL+NFG SEEMHNPV V V + IVL
Sbjct: 219  LLPTGRKNIFYLSIYGSVLGAGTFILHHISMLVNSILINFGLSEEMHNPVYVFVVVGIVL 278

Query: 915  AGAALGYWLVRKFVIXXXXXXXXXXAQFVKWAMRIIGFTCIFQSTLDTPLALAAQCCCLM 1094
            AGAALGYW+VRKFVI          AQFVKWAMRI+  T IFQSTLD  LAL       +
Sbjct: 279  AGAALGYWIVRKFVISKDGSVDVGIAQFVKWAMRIVAATLIFQSTLDILLALITFQSLYV 338

Query: 1095 ICFTFSRFKWHSLEHLSTHGNRNHQGPTRG---------RATVKHTRAEFLSRPGTTSRR 1247
            +CF  +           T  N+ +Q  +RG         + T KH R EFLSR G  S  
Sbjct: 339  VCFLINGL---------TRRNQVYQSHSRGGSPWLQQGRQVTAKHNRTEFLSRSGKMSSG 389

Query: 1248 GNIWNSPNSTKSSAKWT 1298
            G +W SP   KS++ W+
Sbjct: 390  GKMWGSP---KSASGWS 403



 Score = 82.4 bits (202), Expect(2) = e-136
 Identities = 31/54 (57%), Positives = 46/54 (85%)
 Frame = +1

Query: 1405 TFHKTPNRKKFTKQEWEDFTEESTRQALADWASTPEFTDWIVKNADRVKVVPEE 1566
            TFHKTP +KKFT+ EWEDFT +ST+QA+ +WA++PE   W+V+NADR++++P +
Sbjct: 419  TFHKTPKQKKFTETEWEDFTRDSTQQAVGEWAASPEVAAWLVENADRIQLIPSD 472


>ref|XP_003624694.1| hypothetical protein MTR_7g086460 [Medicago truncatula]
            gi|87162736|gb|ABD28531.1| protein F1K23.6 - , putative
            [Medicago truncatula] gi|355499709|gb|AES80912.1|
            hypothetical protein MTR_7g086460 [Medicago truncatula]
          Length = 518

 Score =  414 bits (1063), Expect(2) = e-135
 Identities = 217/378 (57%), Positives = 270/378 (71%), Gaps = 7/378 (1%)
 Frame = +3

Query: 183  SSCSSLKGVDVENPIINFTPSLLVGPS--QGSKDAVACERIQVSGKSRLKLESYANTYRI 356
            SS  +LKGVD+E P ++ +PSLL+G S  QG+K+ + CER+QVSG SRLKL SYAN+  +
Sbjct: 34   SSPITLKGVDIERPTLDVSPSLLLGQSNTQGAKNIMRCERVQVSGISRLKLGSYANSLHV 93

Query: 357  SLVPSVVIPERLHSKIQICFHRNASLGSCQCQSDDWKHVHNGLWNSVMSPYEDRYLDVKY 536
            +L PSV IPERLH+KI++CFHRN +LG CQC+ D+W+ V  G+W++VMSPYE RY+DVK 
Sbjct: 94   TLAPSVAIPERLHNKIKVCFHRNNTLGWCQCEKDEWRGVQKGIWSAVMSPYETRYVDVKI 153

Query: 537  SGEVPGSVTVTIEEDFQQWRIFCLVLGFMLQLLAPIVSSWVPFYYSSSMAIGVFLVVIIL 716
              E+  SVTV ++EDFQQWR+  LV+G +L LLAPIVSSWVPFYYSSSMAIG+FLVVII+
Sbjct: 154  DCEILRSVTVALKEDFQQWRLISLVVGLILLLLAPIVSSWVPFYYSSSMAIGIFLVVIII 213

Query: 717  LFQGMKLLPTGRKNFFYISIYTSFFGAGSYLLHQFSVMINSILLNFGFSEEMHNPVSVLV 896
            LFQGMKLLPTGRKNF YI+IY S  GAGS LLHQFS+++NSIL   G SEEMHNPV++ V
Sbjct: 214  LFQGMKLLPTGRKNFLYITIYGSVLGAGSILLHQFSMIVNSILQTLGMSEEMHNPVALSV 273

Query: 897  FLLIVLAGAALGYWLVRKFVI-XXXXXXXXXXAQFVKWAMRIIGFTCIFQSTLDTPLALA 1073
             L I+LAGAALGYW+VRKFVI           AQFVKWAMR+IG T + QSTLD  LA+ 
Sbjct: 274  LLGIILAGAALGYWIVRKFVISKEDGSVDAGVAQFVKWAMRVIGTTFVLQSTLDNTLAVG 333

Query: 1074 AQCCCLMICFTFSRF----KWHSLEHLSTHGNRNHQGPTRGRATVKHTRAEFLSRPGTTS 1241
            A   C  +C   S      +W+       +     +G TRG       RAEFLS+   + 
Sbjct: 334  ALVFCGAVCKLISSIQLLHEWYETSENDDYSLEWVRG-TRG-------RAEFLSK---SP 382

Query: 1242 RRGNIWNSPNSTKSSAKW 1295
             +G +WNSP S   +  W
Sbjct: 383  PKGKMWNSPKSGSKAKMW 400



 Score = 95.1 bits (235), Expect(2) = e-135
 Identities = 39/54 (72%), Positives = 50/54 (92%)
 Frame = +1

Query: 1405 TFHKTPNRKKFTKQEWEDFTEESTRQALADWASTPEFTDWIVKNADRVKVVPEE 1566
            TFHKT NRKKFTK+EW+DFT +ST+QALA+WA++PEFTDWIV +ADR+K++P E
Sbjct: 439  TFHKTGNRKKFTKEEWDDFTRDSTKQALAEWAASPEFTDWIVDHADRIKLLPSE 492