BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00011505
         (645 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ADG02944.1| putative C-8,7 sterol isomerase [Gossypium hirsutum]   251   1e-64
ref|XP_002285453.1| PREDICTED: probable 3-beta-hydroxysteroid-De...   249   2e-64
ref|XP_003534175.1| PREDICTED: probable 3-beta-hydroxysteroid-De...   247   2e-63
gb|ACU24634.1| unknown [Glycine max]                                  247   2e-63
gb|ABU95101.1| putative C-8,7 sterol isomerase [Solanum tuberosum]    247   2e-63

>gb|ADG02944.1| putative C-8,7 sterol isomerase [Gossypium hirsutum]
          Length = 217

 Score =  251 bits (640), Expect = 1e-64
 Identities = 122/181 (67%), Positives = 135/181 (74%)
 Frame = +2

Query: 101 HPYVPEDLSLPGFVPGFLPMSTXXXXXXXXXXXXXXXXXXXXXRVAKLAKLDRLLMCWWV 280
           HPYVP DL LPG+VP  L  ST                     R   ++KLDRLLMCWW 
Sbjct: 3   HPYVPRDLQLPGYVPVSLSQSTILTVYGLSSLLVVSLVWFLSGRSRSISKLDRLLMCWWA 62

Query: 281 FTGLTHIILEGYFAFSPKFYQEKTPHYLAEVWKEYSKGDSRYAGRDSGVVAVEAITAVLE 460
           FTGLTHIILEGYFAFSP+FY++KT  YLAEVWKEYSKGDSRYAGRDS +VAVE IT+VLE
Sbjct: 63  FTGLTHIILEGYFAFSPEFYKDKTGFYLAEVWKEYSKGDSRYAGRDSTIVAVEGITSVLE 122

Query: 461 GPACLLVAYAIAKRKSYSNILQVAISLGQLYGTAVYFITSYLEGDNFATNSFYYYSYYIF 640
           GP CLL  YAIAK K YS ILQ AISLGQLYGT VYFIT+YLEGDNF+ + FYYY+YY+ 
Sbjct: 123 GPPCLLAVYAIAKGKGYSYILQFAISLGQLYGTFVYFITAYLEGDNFSASPFYYYAYYVL 182

Query: 641 A 643
           A
Sbjct: 183 A 183


>ref|XP_002285453.1| PREDICTED: probable
           3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase
           isoform 2 [Vitis vinifera]
           gi|225435456|ref|XP_002285450.1| PREDICTED: probable
           3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase
           isoform 1 [Vitis vinifera] gi|297746323|emb|CBI16379.3|
           unnamed protein product [Vitis vinifera]
          Length = 222

 Score =  249 bits (637), Expect = 2e-64
 Identities = 119/182 (65%), Positives = 138/182 (75%)
 Frame = +2

Query: 98  AHPYVPEDLSLPGFVPGFLPMSTXXXXXXXXXXXXXXXXXXXXXRVAKLAKLDRLLMCWW 277
           +HPYVP+DL LPG+VP  L  ST                     R  K +K+DRLLMCWW
Sbjct: 7   SHPYVPKDLKLPGYVPVVLSQSTIVGVYGLSSLLVVSVIWIISGRFPKTSKIDRLLMCWW 66

Query: 278 VFTGLTHIILEGYFAFSPKFYQEKTPHYLAEVWKEYSKGDSRYAGRDSGVVAVEAITAVL 457
            FTGLTH ILEGYF FSP+FY++KT  YLAEVWKEYSKGDSRYA RD+GV+AVE ITAVL
Sbjct: 67  AFTGLTHAILEGYFVFSPEFYKDKTACYLAEVWKEYSKGDSRYAARDAGVIAVEGITAVL 126

Query: 458 EGPACLLVAYAIAKRKSYSNILQVAISLGQLYGTAVYFITSYLEGDNFATNSFYYYSYYI 637
           EGPA LL  Y IA ++SYS ILQ+ ISLGQLYGTAVYFITSYL+GDNFA +++YYY+YY+
Sbjct: 127 EGPASLLALYTIATKRSYSYILQLTISLGQLYGTAVYFITSYLDGDNFAASAYYYYAYYV 186

Query: 638 FA 643
           FA
Sbjct: 187 FA 188


>ref|XP_003534175.1| PREDICTED: probable
           3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase-like
           [Glycine max]
          Length = 219

 Score =  247 bits (630), Expect = 2e-63
 Identities = 124/182 (68%), Positives = 136/182 (74%)
 Frame = +2

Query: 98  AHPYVPEDLSLPGFVPGFLPMSTXXXXXXXXXXXXXXXXXXXXXRVAKLAKLDRLLMCWW 277
           AHPYVP DL LPG+ P FL MS                      R  K  K+DR+LMCWW
Sbjct: 3   AHPYVPRDLHLPGYAPCFLSMSNILSVFASSSLLIVTLVWIFSGRFKK-TKVDRVLMCWW 61

Query: 278 VFTGLTHIILEGYFAFSPKFYQEKTPHYLAEVWKEYSKGDSRYAGRDSGVVAVEAITAVL 457
            FTGLTHIILEGYF FSP+F+++KT  YLAEVWKEYSKGDSRYAGRD+GVV VE ITAVL
Sbjct: 62  AFTGLTHIILEGYFVFSPEFFKDKTGFYLAEVWKEYSKGDSRYAGRDAGVVTVEGITAVL 121

Query: 458 EGPACLLVAYAIAKRKSYSNILQVAISLGQLYGTAVYFITSYLEGDNFATNSFYYYSYYI 637
           EGPA LL  YAIA  KSYS ILQ AISLGQLYGTAVY+IT+ LEGDNF+TNSFYYY+YYI
Sbjct: 122 EGPASLLAVYAIATGKSYSYILQFAISLGQLYGTAVYYITAILEGDNFSTNSFYYYAYYI 181

Query: 638 FA 643
            A
Sbjct: 182 GA 183


>gb|ACU24634.1| unknown [Glycine max]
          Length = 219

 Score =  247 bits (630), Expect = 2e-63
 Identities = 124/182 (68%), Positives = 136/182 (74%)
 Frame = +2

Query: 98  AHPYVPEDLSLPGFVPGFLPMSTXXXXXXXXXXXXXXXXXXXXXRVAKLAKLDRLLMCWW 277
           AHPYVP DL LPG+ P FL MS                      R  K  K+DR+LMCWW
Sbjct: 3   AHPYVPRDLHLPGYAPCFLSMSNILSVFASSSLLIVTLVWIFSGRFKK-TKVDRVLMCWW 61

Query: 278 VFTGLTHIILEGYFAFSPKFYQEKTPHYLAEVWKEYSKGDSRYAGRDSGVVAVEAITAVL 457
            FTGLTHIILEGYF FSP+F+++KT  YLAEVWKEYSKGDSRYAGRD+GVV VE ITAVL
Sbjct: 62  AFTGLTHIILEGYFVFSPEFFKDKTGFYLAEVWKEYSKGDSRYAGRDAGVVTVEGITAVL 121

Query: 458 EGPACLLVAYAIAKRKSYSNILQVAISLGQLYGTAVYFITSYLEGDNFATNSFYYYSYYI 637
           EGPA LL  YAIA  KSYS ILQ AISLGQLYGTAVY+IT+ LEGDNF+TNSFYYY+YYI
Sbjct: 122 EGPASLLAVYAIAAGKSYSYILQFAISLGQLYGTAVYYITAILEGDNFSTNSFYYYAYYI 181

Query: 638 FA 643
            A
Sbjct: 182 GA 183


>gb|ABU95101.1| putative C-8,7 sterol isomerase [Solanum tuberosum]
          Length = 221

 Score =  247 bits (630), Expect = 2e-63
 Identities = 114/185 (61%), Positives = 139/185 (75%)
 Frame = +2

Query: 89  QEAAHPYVPEDLSLPGFVPGFLPMSTXXXXXXXXXXXXXXXXXXXXXRVAKLAKLDRLLM 268
           Q  AHPYVP DL LPGFVP FL  S                       V KL+K DR+LM
Sbjct: 4   QGEAHPYVPHDLKLPGFVPLFLSQSEIVGVYGISSLLVVVFMWVFSGFVPKLSKTDRVLM 63

Query: 269 CWWVFTGLTHIILEGYFAFSPKFYQEKTPHYLAEVWKEYSKGDSRYAGRDSGVVAVEAIT 448
           CWW+FTGLTH++LEGYF F+P FYQ+ +P YLAEVWKEYSKGDSRY GRD+ V++VE +T
Sbjct: 64  CWWIFTGLTHMVLEGYFVFTPDFYQKTSPVYLAEVWKEYSKGDSRYVGRDAAVLSVEGVT 123

Query: 449 AVLEGPACLLVAYAIAKRKSYSNILQVAISLGQLYGTAVYFITSYLEGDNFATNSFYYYS 628
           AV+EGPACLL  YAIA +K+Y ++LQ++I LGQLYGTA+YFIT+ LEGDNF+T+ FYYY+
Sbjct: 124 AVIEGPACLLAVYAIATKKAYRHVLQLSICLGQLYGTAIYFITAVLEGDNFSTSPFYYYA 183

Query: 629 YYIFA 643
           YY+FA
Sbjct: 184 YYVFA 188