BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00011303
         (1705 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002278843.1| PREDICTED: l-2-hydroxyglutarate dehydrogenas...   307   e-137
ref|XP_003618905.1| L-2-hydroxyglutarate dehydrogenase [Medicago...   311   e-133
ref|XP_002308844.1| predicted protein [Populus trichocarpa] gi|2...   300   e-132
gb|AFK34722.1| unknown [Lotus japonicus]                              296   e-130
ref|XP_003525735.1| PREDICTED: l-2-hydroxyglutarate dehydrogenas...   286   e-129

>ref|XP_002278843.1| PREDICTED: l-2-hydroxyglutarate dehydrogenase, mitochondrial [Vitis
           vinifera] gi|297741744|emb|CBI32876.3| unnamed protein
           product [Vitis vinifera]
          Length = 424

 Score =  307 bits (787), Expect(2) = e-137
 Identities = 146/199 (73%), Positives = 167/199 (83%)
 Frame = -3

Query: 938 GEAEHHGTTFCFNTTVTGGHLEGNQIHLHISGSKDLESWDGRSSLHPELILIPKLVVNSA 759
           GEAE+H TTFC+NT V GGHLEG+ I LHIS SKDLE+WD R  LHPE+IL+ KLVVNSA
Sbjct: 189 GEAENHRTTFCYNTAVIGGHLEGDHICLHISESKDLENWDRRFPLHPEVILMAKLVVNSA 248

Query: 758 GLSAPVLAKRFSGLDNRAIPRSYFARGSYFTVTNIKSPPFKHLIYPIPEDGGLGVHVTLD 579
           GLSAP LAKRF GL++  IP S++ARG YF ++ +K+PPFKHLIYPIPEDGGLGVHVTLD
Sbjct: 249 GLSAPALAKRFDGLNSTVIPASHYARGCYFVLSKMKTPPFKHLIYPIPEDGGLGVHVTLD 308

Query: 578 LNGQVKFGPDVEWIDGIDDISNFLNKFNYSVCADRANQFYHEIRKYYPELKDGSLEPSYA 399
           L+GQ+KFGPDVEWI+G DDIS+FLNKF+YSVCA R  +FY EIRKYYP LKD SLEP YA
Sbjct: 309 LDGQIKFGPDVEWINGADDISSFLNKFDYSVCAKRVERFYPEIRKYYPNLKDDSLEPGYA 368

Query: 398 GIRPKLSGPGHGFSDFVIK 342
           GIRPKLSG      DFVI+
Sbjct: 369 GIRPKLSGAQQSAVDFVIQ 387



 Score =  208 bits (529), Expect(2) = e-137
 Identities = 107/164 (65%), Positives = 126/164 (76%)
 Frame = -2

Query: 1488 DNKISKEKVECXXXXXXXXXXXXARELCCKHGKKHVLVLESAATFGTGTSSRNSEVIHAG 1309
            +  +++E V+C            AREL  K   + V+V+E A+TFGTGTSSRNSEVIHAG
Sbjct: 28   ERAVARESVDCVVIGAGVVGIAVARELALKG--REVVVIEFASTFGTGTSSRNSEVIHAG 85

Query: 1308 IYYPTNSLKARFCVRGREMLYNYCKEFEIPHKKIGKLIVATGSSEIPKLNSLMDRGLQNG 1129
            IYYP NSLKA FC RGRE+LY YC E E+PHK+IGKLIVAT SSE+PKLN LM RG +NG
Sbjct: 86   IYYPRNSLKAIFCARGRELLYKYCSEHEVPHKQIGKLIVATRSSEVPKLNDLMIRGNENG 145

Query: 1128 VGGLKMMEGCEAKSLEPGLKCVKALLSSASGIVDTHSLMLSLVG 997
            V GL+M+EG EA  +EP L+CV+ALLS ASGIVDTHSLMLSLVG
Sbjct: 146  VDGLRMLEGFEAMEMEPELQCVRALLSPASGIVDTHSLMLSLVG 189


>ref|XP_003618905.1| L-2-hydroxyglutarate dehydrogenase [Medicago truncatula]
           gi|355493920|gb|AES75123.1| L-2-hydroxyglutarate
           dehydrogenase [Medicago truncatula]
          Length = 483

 Score =  311 bits (798), Expect(2) = e-133
 Identities = 146/199 (73%), Positives = 173/199 (86%)
 Frame = -3

Query: 938 GEAEHHGTTFCFNTTVTGGHLEGNQIHLHISGSKDLESWDGRSSLHPELILIPKLVVNSA 759
           GEAE+HG TF +N+TV GGH+EGN+I LH+S +K L+ W+G+SSL PEL+LIPKLVVNSA
Sbjct: 249 GEAENHGATFTYNSTVIGGHMEGNEICLHVSETKSLKEWNGKSSLQPELVLIPKLVVNSA 308

Query: 758 GLSAPVLAKRFSGLDNRAIPRSYFARGSYFTVTNIKSPPFKHLIYPIPEDGGLGVHVTLD 579
           GLSA  LAKRF+GL+N+ IP +Y+ARG YFT++N K+ PF+HLIYPIPEDGGLGVHVTLD
Sbjct: 309 GLSALALAKRFTGLENKVIPPAYYARGCYFTLSNTKASPFRHLIYPIPEDGGLGVHVTLD 368

Query: 578 LNGQVKFGPDVEWIDGIDDISNFLNKFNYSVCADRANQFYHEIRKYYPELKDGSLEPSYA 399
           LNGQVKFGPDVEWIDG+DDIS+F NKF+YSV A+RA +FY EIRKYYP LKDGSLEP Y+
Sbjct: 369 LNGQVKFGPDVEWIDGVDDISSFQNKFDYSVQANRAEKFYPEIRKYYPNLKDGSLEPGYS 428

Query: 398 GIRPKLSGPGHGFSDFVIK 342
           GIRPKLSGP     DFVI+
Sbjct: 429 GIRPKLSGPCQSPVDFVIQ 447



 Score =  192 bits (489), Expect(2) = e-133
 Identities = 101/193 (52%), Positives = 135/193 (69%)
 Frame = -2

Query: 1578 RNMKASCSYNNNYYRTFKRESESVNCNYYNDNKISKEKVECXXXXXXXXXXXXARELCCK 1399
            RN++++   + N   ++    E+          + +E+V+C            AR L  K
Sbjct: 38   RNIRSTTQNDTNTTTSYPVSRETTT-----SYSVPRERVDCVVIGAGVVGIAVARALALK 92

Query: 1398 HGKKHVLVLESAATFGTGTSSRNSEVIHAGIYYPTNSLKARFCVRGREMLYNYCKEFEIP 1219
               + V+V+ESA +FGTGTSSRNSEV+HAGIYYP +SLKA FCV+GREMLY YC + +IP
Sbjct: 93   G--REVIVIESAPSFGTGTSSRNSEVVHAGIYYPHHSLKAIFCVKGREMLYEYCAKHDIP 150

Query: 1218 HKKIGKLIVATGSSEIPKLNSLMDRGLQNGVGGLKMMEGCEAKSLEPGLKCVKALLSSAS 1039
            H++ GKLIVAT SSEIPKL+ +++ G+QNGV GLKMM+G +A  +EP L+CVKA+LS  S
Sbjct: 151  HEQTGKLIVATRSSEIPKLSVILNHGIQNGVDGLKMMDGVDAMKMEPELQCVKAILSPLS 210

Query: 1038 GIVDTHSLMLSLV 1000
            GIVD+HSLMLSLV
Sbjct: 211  GIVDSHSLMLSLV 223


>ref|XP_002308844.1| predicted protein [Populus trichocarpa] gi|222854820|gb|EEE92367.1|
           predicted protein [Populus trichocarpa]
          Length = 430

 Score =  300 bits (769), Expect(2) = e-132
 Identities = 141/199 (70%), Positives = 168/199 (84%)
 Frame = -3

Query: 938 GEAEHHGTTFCFNTTVTGGHLEGNQIHLHISGSKDLESWDGRSSLHPELILIPKLVVNSA 759
           GEAE++GTTF +N+TV  GHLEGN +HL+I  SKDLE+W+G   LHPEL+L+PKLVVNSA
Sbjct: 194 GEAENNGTTFSYNSTVISGHLEGNCLHLYIVESKDLENWNGDYPLHPELVLVPKLVVNSA 253

Query: 758 GLSAPVLAKRFSGLDNRAIPRSYFARGSYFTVTNIKSPPFKHLIYPIPEDGGLGVHVTLD 579
           GLS+  LAKRF GLDN  IP  +FARG YFT+++ K PPF+HLIYPIPEDGGLGVHVTLD
Sbjct: 254 GLSSLALAKRFHGLDNGIIPPGFFARGCYFTLSSTKVPPFEHLIYPIPEDGGLGVHVTLD 313

Query: 578 LNGQVKFGPDVEWIDGIDDISNFLNKFNYSVCADRANQFYHEIRKYYPELKDGSLEPSYA 399
           L+G +KFGPDVEWIDGIDD+S+FLNK++YSV A RA +FY EIRKYYP LKDGSL+P Y+
Sbjct: 314 LDGHLKFGPDVEWIDGIDDVSSFLNKYDYSVSASRAERFYPEIRKYYPNLKDGSLQPGYS 373

Query: 398 GIRPKLSGPGHGFSDFVIK 342
           GIRPK+SGP     DFVI+
Sbjct: 374 GIRPKISGPRQSPIDFVIQ 392



 Score =  198 bits (503), Expect(2) = e-132
 Identities = 106/161 (65%), Positives = 120/161 (74%)
 Frame = -2

Query: 1479 ISKEKVECXXXXXXXXXXXXARELCCKHGKKHVLVLESAATFGTGTSSRNSEVIHAGIYY 1300
            +  EK++C            AREL  K   + VLVL+SA+TFGTGTSSRNSEVIHAGIYY
Sbjct: 36   VPTEKLDCVVIGAGVVGIAIARELASKG--REVLVLDSASTFGTGTSSRNSEVIHAGIYY 93

Query: 1299 PTNSLKARFCVRGREMLYNYCKEFEIPHKKIGKLIVATGSSEIPKLNSLMDRGLQNGVGG 1120
            P +SLKA FCVRGRE+LY YC E  +P K+IGKLIVATG  EI KLN LMDRG QNGV  
Sbjct: 94   PPDSLKALFCVRGRELLYRYCYEHGVPCKQIGKLIVATGPLEISKLNELMDRGTQNGVDN 153

Query: 1119 LKMMEGCEAKSLEPGLKCVKALLSSASGIVDTHSLMLSLVG 997
            LKM+EG EA  +EP L+C KALLS  SGIVD+HSLMLSLVG
Sbjct: 154  LKMLEGFEAMKMEPELQCKKALLSPVSGIVDSHSLMLSLVG 194


>gb|AFK34722.1| unknown [Lotus japonicus]
          Length = 418

 Score =  296 bits (758), Expect(2) = e-130
 Identities = 141/199 (70%), Positives = 164/199 (82%)
 Frame = -3

Query: 938 GEAEHHGTTFCFNTTVTGGHLEGNQIHLHISGSKDLESWDGRSSLHPELILIPKLVVNSA 759
           GEAE+H TTF +N+TV GGHLEG +I LHI  +K L+ WDG S LHPELIL+PKL+VNS 
Sbjct: 183 GEAENHRTTFTYNSTVIGGHLEGKEICLHILETKCLKEWDGTSILHPELILVPKLLVNST 242

Query: 758 GLSAPVLAKRFSGLDNRAIPRSYFARGSYFTVTNIKSPPFKHLIYPIPEDGGLGVHVTLD 579
           GLSAP LAKRF+ L +  IP SY+ARG YFT+++ K+ PF+HLIYPIPEDGGLGVHVTLD
Sbjct: 243 GLSAPALAKRFTSLQSEVIPPSYYARGCYFTLSDTKASPFRHLIYPIPEDGGLGVHVTLD 302

Query: 578 LNGQVKFGPDVEWIDGIDDISNFLNKFNYSVCADRANQFYHEIRKYYPELKDGSLEPSYA 399
           LNGQ+KFGPDVEWIDG+DDIS+F NKF+YSV A+RA QFY  IR YYP LKD +LEP YA
Sbjct: 303 LNGQIKFGPDVEWIDGVDDISSFQNKFDYSVHANRAEQFYPAIRSYYPNLKDRALEPGYA 362

Query: 398 GIRPKLSGPGHGFSDFVIK 342
           GIRPKLS PG    DFVI+
Sbjct: 363 GIRPKLSRPGQSPVDFVIQ 381



 Score =  198 bits (503), Expect(2) = e-130
 Identities = 101/163 (61%), Positives = 123/163 (75%)
 Frame = -2

Query: 1485 NKISKEKVECXXXXXXXXXXXXARELCCKHGKKHVLVLESAATFGTGTSSRNSEVIHAGI 1306
            + + +EKV+C            AR L  K   + VLV+ES +TFGTGTSSRNSEVIHAGI
Sbjct: 23   DSVPREKVDCVVIGAGVVGIAVARALALKG--REVLVIESGSTFGTGTSSRNSEVIHAGI 80

Query: 1305 YYPTNSLKARFCVRGREMLYNYCKEFEIPHKKIGKLIVATGSSEIPKLNSLMDRGLQNGV 1126
            YYP +SLKA FCVRGREMLY YC   +IPHK++GKLIVAT SSEIPKLN +++ G+QNGV
Sbjct: 81   YYPRDSLKAIFCVRGREMLYEYCSMHDIPHKQVGKLIVATRSSEIPKLNDILNHGIQNGV 140

Query: 1125 GGLKMMEGCEAKSLEPGLKCVKALLSSASGIVDTHSLMLSLVG 997
              L+M+ G +A  +EP L+CVKA+LS  SGIVD+HSLMLSLVG
Sbjct: 141  DSLEMINGAKAMKMEPELQCVKAILSPLSGIVDSHSLMLSLVG 183


>ref|XP_003525735.1| PREDICTED: l-2-hydroxyglutarate dehydrogenase, mitochondrial-like
           [Glycine max]
          Length = 410

 Score =  286 bits (731), Expect(2) = e-129
 Identities = 135/199 (67%), Positives = 165/199 (82%)
 Frame = -3

Query: 938 GEAEHHGTTFCFNTTVTGGHLEGNQIHLHISGSKDLESWDGRSSLHPELILIPKLVVNSA 759
           GEAE+ GTTF +N+TV GGHLEG++I LH++ +  L  W G S L PEL+LIPKLVVNS 
Sbjct: 176 GEAENQGTTFTYNSTVIGGHLEGSEICLHVTETDRLTEWKGTSILQPELLLIPKLVVNST 235

Query: 758 GLSAPVLAKRFSGLDNRAIPRSYFARGSYFTVTNIKSPPFKHLIYPIPEDGGLGVHVTLD 579
           GLS+PVLAKRF+G  +  +P +Y+ARG YFT++N K+ PF+ LIYPIPEDGG+GVHVT+D
Sbjct: 236 GLSSPVLAKRFNGPKSGVVPPAYYARGCYFTLSNTKNSPFRRLIYPIPEDGGIGVHVTID 295

Query: 578 LNGQVKFGPDVEWIDGIDDISNFLNKFNYSVCADRANQFYHEIRKYYPELKDGSLEPSYA 399
           LNGQVKFGP+VEWID +DDIS+F NK++YSV A+RA +FY EIRKYYP LKDGSLEP Y+
Sbjct: 296 LNGQVKFGPNVEWIDSVDDISSFQNKYDYSVNANRAERFYPEIRKYYPNLKDGSLEPGYS 355

Query: 398 GIRPKLSGPGHGFSDFVIK 342
           GIRPKLSGP    SDFVI+
Sbjct: 356 GIRPKLSGPLQPPSDFVIQ 374



 Score =  204 bits (520), Expect(2) = e-129
 Identities = 108/170 (63%), Positives = 128/170 (75%), Gaps = 5/170 (2%)
 Frame = -2

Query: 1491 NDNK-----ISKEKVECXXXXXXXXXXXXARELCCKHGKKHVLVLESAATFGTGTSSRNS 1327
            NDN+     + KE+VEC            AR L  K   + VLV+ESA TFGTGTSSRNS
Sbjct: 9    NDNRTTWDSVPKERVECVVIGAGVVGIAVARALALKG--REVLVVESAPTFGTGTSSRNS 66

Query: 1326 EVIHAGIYYPTNSLKARFCVRGREMLYNYCKEFEIPHKKIGKLIVATGSSEIPKLNSLMD 1147
            EVIHAGIYYP NS KA FCVRGREMLY YC + +IPHK+IGKLIVAT SSEIPKLN +++
Sbjct: 67   EVIHAGIYYPLNSFKAIFCVRGREMLYEYCSKHDIPHKQIGKLIVATRSSEIPKLNDILN 126

Query: 1146 RGLQNGVGGLKMMEGCEAKSLEPGLKCVKALLSSASGIVDTHSLMLSLVG 997
             G+QNGV GLK+++G EA  +EP L+CVKA+LS  +GIVD+HSLMLSLVG
Sbjct: 127  CGIQNGVDGLKIVDGVEAMKMEPELQCVKAILSPLTGIVDSHSLMLSLVG 176