BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00011224
         (1050 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAD56579.1|AF184272_1 dihydroflavonol 4-reductase like [Daucu...   241   3e-61
emb|CAN80655.1| hypothetical protein VITISV_011650 [Vitis vinifera]   227   3e-57
emb|CBI19727.3| unnamed protein product [Vitis vinifera]              216   7e-54
ref|XP_002281250.2| PREDICTED: bifunctional dihydroflavonol 4-re...   173   7e-41
ref|XP_002281758.2| PREDICTED: dihydroflavonol-4-reductase-like ...   173   7e-41

>gb|AAD56579.1|AF184272_1 dihydroflavonol 4-reductase like [Daucus carota]
          Length = 332

 Score =  241 bits (614), Expect = 3e-61
 Identities = 120/179 (67%), Positives = 145/179 (81%)
 Frame = -1

Query: 741 MYVVAKTSAEKAAWEYAEKNGIDMISILPSLVLGHFISPSISFSVEAANAIYTAITSCSK 562
           MYVVAKT+AE+AAW+YAE+NGIDM+++ PS+VLGHF +P  SFS EAA A+YT      K
Sbjct: 163 MYVVAKTTAERAAWKYAEENGIDMVTVHPSIVLGHFNTPHTSFSTEAATALYT------K 216

Query: 561 DESTMAFLKRMNATPAVHLDDVCNAEIHLFEHPHAKGRYICSSHTFDIRDIAHSLSLKYP 382
           +E+ MA LK+++ +PAVHLDDVCNA I+LFEHP AKGRYICSSHT+++ +I HSLSLKYP
Sbjct: 217 NEANMALLKKLHGSPAVHLDDVCNAHIYLFEHPLAKGRYICSSHTYNVFEIGHSLSLKYP 276

Query: 381 DKNIQTEFEGLNEDVPNEDVPCSSRKLMMETGFEFAHKNKDVGDLCAETVESCRAKGLL 205
           ++NI TEFEGL  D      P SS+KL M  GFEFAHKNK VGDLCAET+ESCR KGLL
Sbjct: 277 ERNIPTEFEGL--DKSQRINPVSSKKL-MSLGFEFAHKNKSVGDLCAETIESCREKGLL 332



 Score =  154 bits (390), Expect = 3e-35
 Identities = 76/91 (83%), Positives = 80/91 (87%)
 Frame = -1

Query: 1017 TVCVTGASGYIGSWLVMRLLERGYHVRATVRDPGNIRKVKHLLELPYASTNLSLWKADFA 838
            TVCVTGASGYIGSWLV  LLERGYHVRATVRDPGN RKVK+LLELP AST+LSLWKAD A
Sbjct: 4    TVCVTGASGYIGSWLVKTLLERGYHVRATVRDPGNERKVKNLLELPNASTHLSLWKADLA 63

Query: 837  EESSYDDALQGCDGLFHVAAPMELLNLQEQA 745
            EESSYDDA+QGC G+FHVA PMELL   E A
Sbjct: 64   EESSYDDAVQGCHGVFHVATPMELLYQDEPA 94


>emb|CAN80655.1| hypothetical protein VITISV_011650 [Vitis vinifera]
          Length = 440

 Score =  227 bits (579), Expect = 3e-57
 Identities = 125/244 (51%), Positives = 166/244 (68%), Gaps = 24/244 (9%)
 Frame = -1

Query: 1017 TVCVTGASGYIGSWLVMRLLERGYHVRATVRDPGNIRKVKHLLELPYASTNLSLWKADFA 838
            TVCVTGASG+IGSWLVM+LL+RGY+V ATVRDPGN+ KVKHLLELP AST+LSLW+AD  
Sbjct: 181  TVCVTGASGFIGSWLVMKLLQRGYYVHATVRDPGNVEKVKHLLELPKASTHLSLWRADLK 240

Query: 837  EESSYDDALQGCDGLFHVAAPMELLNLQEQAGMYVVAKTSAEKAAWEY--AEKNGIDMIS 664
            EE S+DDA+QGC G+FHVA+PM++    + A MY +AKT+AEKAAWE    ++ G+D+++
Sbjct: 241  EEGSFDDAIQGCIGVFHVASPMDIST--QDAQMYFIAKTTAEKAAWEVRRLKEKGLDVVT 298

Query: 663  IL----------------PSLVLGHFISPSI------SFSVEAANAIYTAITSCSKDEST 550
            I                 PS  L +    +I       F+V  +  +Y ++     +E+ 
Sbjct: 299  IRTPCCGGAICYTFKGLPPSAKLDYSRYSNILVRLQKFFAVLVSGNLYISL----GEEAG 354

Query: 549  MAFLKRMNATPAVHLDDVCNAEIHLFEHPHAKGRYICSSHTFDIRDIAHSLSLKYPDKNI 370
               L R     AVH+DD+C+A I+LFEHP AKGRYICSSH F+I ++A SLSLKY + NI
Sbjct: 355  CNLLARGR---AVHVDDLCDAHIYLFEHPEAKGRYICSSHCFNIIELARSLSLKYSEYNI 411

Query: 369  QTEF 358
             T++
Sbjct: 412  PTKY 415



 Score = 73.9 bits (180), Expect = 6e-11
 Identities = 34/49 (69%), Positives = 40/49 (81%)
 Frame = -1

Query: 918 GNIRKVKHLLELPYASTNLSLWKADFAEESSYDDALQGCDGLFHVAAPM 772
           GN  KVKHLLEL  AST+ SLW+AD  EE S+DDA+QGC G+FHVA+PM
Sbjct: 30  GNAEKVKHLLELHKASTHPSLWRADLNEEGSFDDAIQGCIGVFHVASPM 78


>emb|CBI19727.3| unnamed protein product [Vitis vinifera]
          Length = 931

 Score =  216 bits (550), Expect = 7e-54
 Identities = 136/334 (40%), Positives = 190/334 (56%), Gaps = 63/334 (18%)
 Frame = -1

Query: 1017 TVCVTGASGYIGSWLVMRLLERGYHVRATVRDPGNIRKVKHLLELPYASTNLSLWKA--- 847
            TVCVTGASG+IGSWLVMRLLERGY VRATVRDP N++KVKHLL+LP A T+L+LWKA   
Sbjct: 601  TVCVTGASGFIGSWLVMRLLERGYTVRATVRDPTNVKKVKHLLDLPKAETHLTLWKADLA 660

Query: 846  ----------------------DFAEESSYDDAL----QGCDGLFHVAAPMELL------ 763
                                  DF  +   ++ +    +G  G+    A  + +      
Sbjct: 661  DEGSFDEAIKGCTGVFHVATPMDFESKDPENEVIKPTIEGMLGIMKSCAAAKTVRRLVFT 720

Query: 762  ------NLQEQ----------------------AGMYVVAKTSAEKAAWEYAEKNGIDMI 667
                  N+QE                       A MY V+KT AE+AAW+YA++N ID I
Sbjct: 721  SSAGTVNIQEHQLPVYDESCWSDMEFCRAKKMTAWMYFVSKTLAEQAAWKYAKENNIDFI 780

Query: 666  SILPSLVLGHFISPSISFSVEAANAIYTAITSCSKDESTMAFLKRMNATPAVHLDDVCNA 487
            +I+P+LV+G FI  S+  S      + TA++  + +E+  + +++      VHLDD+CNA
Sbjct: 781  TIIPTLVVGPFIMSSMPPS------LITALSPITGNEAHYSIIRQGQF---VHLDDLCNA 831

Query: 486  EIHLFEHPHAKGRYICSSHTFDIRDIAHSLSLKYPDKNIQTEFEGLNEDVPNEDVPCSSR 307
             I+LFE+P A+GRYICSSH   I D+A  L  KYP+ NI TEF+G++E++ +    C S 
Sbjct: 832  HIYLFENPKAEGRYICSSHDCIILDLAKMLREKYPEYNIPTEFKGVDENLKS---VCFSS 888

Query: 306  KLMMETGFEFAHKNKDVGDLCAETVESCRAKGLL 205
            K + + GFEF +    + D+    V++CRAKGLL
Sbjct: 889  KKLTDLGFEFKY---SLEDMFTGAVDTCRAKGLL 919



 Score =  173 bits (438), Expect = 7e-41
 Identities = 88/183 (48%), Positives = 132/183 (72%), Gaps = 2/183 (1%)
 Frame = -1

Query: 747 AGMYVVAKTSAEKAAWEYAEKNGIDMISILPSLVLGHFISPSISFSVEAANAIYTAITSC 568
           A MY +AKT+AEKAAWE+A++ G+D+++I P +V+G F++PS+  S +   A+ T     
Sbjct: 162 AWMYFIAKTTAEKAAWEFAKEKGLDVVTIQPPVVVGPFVTPSLPPSAKLVLAVLTG---- 217

Query: 567 SKDESTMAFLKRMNATPAVHLDDVCNAEIHLFEHPHAKGRYICSSHTFDIRDIAHSLSLK 388
             +E+    L R  A   VH+DD+C+A I+LFEHP AKGRYICSSH F+I ++A SLSLK
Sbjct: 218 --EEAGCNLLARGRA---VHVDDLCDAHIYLFEHPEAKGRYICSSHCFNIIELARSLSLK 272

Query: 387 YPDKNIQTEFEGLNEDVPNEDVPCSSRKLMMETGFEFAHKNK--DVGDLCAETVESCRAK 214
           Y + NI T+FEG++E +  + +PCSSRKL ++ G++F + ++  D+GDLC+  +ESC+ K
Sbjct: 273 YSEYNIPTKFEGVDESL--KSIPCSSRKL-LDLGYKFKYNSEEYDIGDLCSGAIESCKEK 329

Query: 213 GLL 205
           GL+
Sbjct: 330 GLM 332



 Score =  143 bits (360), Expect = 8e-32
 Identities = 64/84 (76%), Positives = 77/84 (91%)
 Frame = -1

Query: 1017 TVCVTGASGYIGSWLVMRLLERGYHVRATVRDPGNIRKVKHLLELPYASTNLSLWKADFA 838
            TVCVTGASG+IGSWLVM+LL+RGY+V ATVRDPGN+ KVKHLLELP AST+LSLW+AD  
Sbjct: 8    TVCVTGASGFIGSWLVMKLLQRGYYVHATVRDPGNVEKVKHLLELPKASTHLSLWRADLK 67

Query: 837  EESSYDDALQGCDGLFHVAAPMEL 766
            EE S+DDA+QGC G+FHVA+PM++
Sbjct: 68   EEGSFDDAIQGCIGVFHVASPMDI 91


>ref|XP_002281250.2| PREDICTED: bifunctional dihydroflavonol 4-reductase/flavanone
           4-reductase-like [Vitis vinifera]
          Length = 430

 Score =  173 bits (438), Expect = 7e-41
 Identities = 91/183 (49%), Positives = 128/183 (69%), Gaps = 2/183 (1%)
 Frame = -1

Query: 747 AGMYVVAKTSAEKAAWEYAEKNGIDMISILPSLVLGHFISPSISFSVEAANAIYTAITSC 568
           A MY VAKT+AEK AWE+A++ G+D+++I P  V+G FISPS+S   + + A+ T     
Sbjct: 254 AWMYYVAKTTAEKVAWEFAKEKGLDLVTIHPPFVIGPFISPSLSVGAKISLALLTG---- 309

Query: 567 SKDESTMAFLKRMNATPAVHLDDVCNAEIHLFEHPHAKGRYICSSHTFDIRDIAHSLSLK 388
             DE +   L R  A   VH++D+CNA I+LFEHP A+GRYICSSH F+I ++A SLS K
Sbjct: 310 --DERSYVLLTRGQA---VHVEDLCNAHIYLFEHPEARGRYICSSHCFEITELARSLSNK 364

Query: 387 YPDKNIQTEFEGLNEDVPNEDVPCSSRKLMMETGFEFAHKNK--DVGDLCAETVESCRAK 214
           YP+ NI  +FEG+  D   + VP SS+KL ++ G++F + ++  D+GD+CAE +ESCR K
Sbjct: 365 YPEYNIPAKFEGM--DQFPKPVPLSSKKL-LDLGYKFQYNSEEYDIGDVCAEAIESCREK 421

Query: 213 GLL 205
           GLL
Sbjct: 422 GLL 424



 Score =  131 bits (329), Expect = 3e-28
 Identities = 59/84 (70%), Positives = 73/84 (86%)
 Frame = -1

Query: 1017 TVCVTGASGYIGSWLVMRLLERGYHVRATVRDPGNIRKVKHLLELPYASTNLSLWKADFA 838
            +VCVTGA+G+IGSWLVMRLL+RGY+V  TVRDP +  KVKHLLELP A T+LSLW+AD  
Sbjct: 100  SVCVTGATGFIGSWLVMRLLQRGYYVHGTVRDPADHGKVKHLLELPKAGTHLSLWRADLK 159

Query: 837  EESSYDDALQGCDGLFHVAAPMEL 766
            EE S+DDA+QGC G+FHVA+PM++
Sbjct: 160  EEGSFDDAIQGCVGVFHVASPMDI 183


>ref|XP_002281758.2| PREDICTED: dihydroflavonol-4-reductase-like [Vitis vinifera]
          Length = 337

 Score =  173 bits (438), Expect = 7e-41
 Identities = 88/183 (48%), Positives = 132/183 (72%), Gaps = 2/183 (1%)
 Frame = -1

Query: 747 AGMYVVAKTSAEKAAWEYAEKNGIDMISILPSLVLGHFISPSISFSVEAANAIYTAITSC 568
           A MY +AKT+AEKAAWE+A++ G+D+++I P +V+G F++PS+  S +   A+ T     
Sbjct: 161 AWMYFIAKTTAEKAAWEFAKEKGLDVVTIQPPVVVGPFVTPSLPPSAKLVLAVLTG---- 216

Query: 567 SKDESTMAFLKRMNATPAVHLDDVCNAEIHLFEHPHAKGRYICSSHTFDIRDIAHSLSLK 388
             +E+    L R  A   VH+DD+C+A I+LFEHP AKGRYICSSH F+I ++A SLSLK
Sbjct: 217 --EEAGCNLLARGRA---VHVDDLCDAHIYLFEHPEAKGRYICSSHCFNIIELARSLSLK 271

Query: 387 YPDKNIQTEFEGLNEDVPNEDVPCSSRKLMMETGFEFAHKNK--DVGDLCAETVESCRAK 214
           Y + NI T+FEG++E +  + +PCSSRKL ++ G++F + ++  D+GDLC+  +ESC+ K
Sbjct: 272 YSEYNIPTKFEGVDESL--KSIPCSSRKL-LDLGYKFKYNSEEYDIGDLCSGAIESCKEK 328

Query: 213 GLL 205
           GL+
Sbjct: 329 GLM 331



 Score =  143 bits (360), Expect = 8e-32
 Identities = 64/84 (76%), Positives = 77/84 (91%)
 Frame = -1

Query: 1017 TVCVTGASGYIGSWLVMRLLERGYHVRATVRDPGNIRKVKHLLELPYASTNLSLWKADFA 838
            TVCVTGASG+IGSWLVM+LL+RGY+V ATVRDPGN+ KVKHLLELP AST+LSLW+AD  
Sbjct: 7    TVCVTGASGFIGSWLVMKLLQRGYYVHATVRDPGNVEKVKHLLELPKASTHLSLWRADLK 66

Query: 837  EESSYDDALQGCDGLFHVAAPMEL 766
            EE S+DDA+QGC G+FHVA+PM++
Sbjct: 67   EEGSFDDAIQGCIGVFHVASPMDI 90