BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00011178
         (1822 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003602077.1| 4-coumarate-coa ligase [Medicago truncatula]...   494   e-156
ref|XP_003528115.1| PREDICTED: 4-coumarate--CoA ligase-like 9-li...   491   e-154
ref|XP_002279522.2| PREDICTED: 4-coumarate--CoA ligase-like 9-li...   499   e-154
ref|XP_002511221.1| AMP dependent CoA ligase, putative [Ricinus ...   484   e-154
ref|XP_003523137.1| PREDICTED: 4-coumarate--CoA ligase-like 9-li...   489   e-153

>ref|XP_003602077.1| 4-coumarate-coa ligase [Medicago truncatula]
            gi|355491125|gb|AES72328.1| 4-coumarate-coa ligase
            [Medicago truncatula]
          Length = 562

 Score =  494 bits (1272), Expect(3) = e-156
 Identities = 250/404 (61%), Positives = 309/404 (76%), Gaps = 4/404 (0%)
 Frame = -2

Query: 1209 AKHLPKLPLGTIVVDSPEFQSLLTSSSETPP--RQINISQSNTAAILYSSGTTGRVKGVE 1036
            A  +PKLPLGT++ DSP F S++ S+++T    R +  SQS+TAAIL+SSGTTGRVKGV 
Sbjct: 160  ATKIPKLPLGTVITDSPSFLSVINSTTDTHTELRPVVTSQSDTAAILFSSGTTGRVKGVL 219

Query: 1035 LTHRNFIVVIANLFHNKISWPE--EELPEQSVALFTLPLFHVFGFFMLIRGFAMGETLVL 862
            LTH NFI +I      K ++ +  E+ P   V+LF LPLFHVFGFFM++R  AMGETLVL
Sbjct: 220  LTHGNFIALIGGFCFMKHAYDDGIEDEPHP-VSLFPLPLFHVFGFFMMVRALAMGETLVL 278

Query: 861  LEKFDFIKMLESVEKYKVTYMPVSPPIVVAMAKSEFVKKYDXXXXXXXXXXXXXXXXXXS 682
            +++FDF  ML++VEKY++TYMPVSPP++ A  KSE VKKYD                  +
Sbjct: 279  MQRFDFEGMLKAVEKYRITYMPVSPPLITAFTKSELVKKYDISSIRLLGSGGAPLGKEVA 338

Query: 681  ERFTARFPNVEIVQXXXXXXXXXXXXGMNSPEEGKRYGSGGRLSANLQGKIVDPGTGEAL 502
            E F A+FPNVEIVQ             M   +E KR+GS GRL+ N++ KIVDP TGEAL
Sbjct: 339  ESFKAKFPNVEIVQGYGLTESGGGAARMIGFDEAKRHGSVGRLAENMEAKIVDPVTGEAL 398

Query: 501  PPGQQGELWLRGPIVMKGYVGDNAATAATLTSDGWLKTGDLCYFDSDGFLYIVDRLKELI 322
             PGQ+GELWLRGP +MKGYVGD+ AT  TL S+GWLKTGDLCYFDSDG+L+IVDRLKELI
Sbjct: 399  SPGQKGELWLRGPTIMKGYVGDDKATVETLDSEGWLKTGDLCYFDSDGYLFIVDRLKELI 458

Query: 321  KYKAYQVAPAELEHIIHSIPGVADVAVIPYPDEDAGQIPMAYVVRSPGSNISEAQIKDFV 142
            KYKAYQV PAELEHI+H+ P +AD AV+PYPDEDAGQIPMA+VVR PGSNI+ AQ+ D+V
Sbjct: 459  KYKAYQVPPAELEHILHTNPEIADAAVVPYPDEDAGQIPMAFVVRKPGSNITAAQVMDYV 518

Query: 141  TRQVSPYKKVRRVAFINAIPKSPAGKILRRELVNHALSAGSARL 10
             +QV+PYKK+RRV+FIN+IPKSPAGKILRRELV+ AL++GS++L
Sbjct: 519  AKQVTPYKKIRRVSFINSIPKSPAGKILRRELVDIALTSGSSKL 562



 Score = 70.9 bits (172), Expect(3) = e-156
 Identities = 43/98 (43%), Positives = 56/98 (57%)
 Frame = -1

Query: 1552 QPLSVTQYADSLLRSSTTFSPETTAFLIDSATSRRVSYSEFXXXXXXXXXXXXXXXXXXX 1373
            +PLS+T YA SLL ++T  S  TTA LID+AT   ++Y  F                   
Sbjct: 43   EPLSLTDYALSLLSTTTAVSNNTTA-LIDAATDHHLTYPTFLRQIKSLSSFLQSHTTLSK 101

Query: 1372 XXXXXQNVALILSPPSLHIPVLYFSLMSLNVVVSPANP 1259
                  +VALIL P S+H+PVLYFSL+SL V ++PANP
Sbjct: 102  G-----HVALILIPSSIHVPVLYFSLLSLGVTIAPANP 134



 Score = 37.0 bits (84), Expect(3) = e-156
 Identities = 13/25 (52%), Positives = 20/25 (80%)
 Frame = -2

Query: 1701 LQQSTGAVDPNSGYCSETKTFYSLR 1627
            +Q  +  +DPN+GYCS ++TF+SLR
Sbjct: 9    MQSQSTTIDPNTGYCSHSRTFHSLR 33


>ref|XP_003528115.1| PREDICTED: 4-coumarate--CoA ligase-like 9-like [Glycine max]
          Length = 597

 Score =  491 bits (1264), Expect(3) = e-154
 Identities = 249/405 (61%), Positives = 311/405 (76%), Gaps = 5/405 (1%)
 Frame = -2

Query: 1209 AKHLPK-LPLGTIVVDSPEFQSLLTSS----SETPPRQINISQSNTAAILYSSGTTGRVK 1045
            AK++P  L  GTI++DSP F S+L ++    +++  R++ +SQS++AAIL+SSGTTGRVK
Sbjct: 196  AKNIPSSLKFGTILLDSPLFLSMLNNNEYVNADSRTRRVEVSQSDSAAILFSSGTTGRVK 255

Query: 1044 GVELTHRNFIVVIANLFHNKISWPEEELPEQSVALFTLPLFHVFGFFMLIRGFAMGETLV 865
            GV LTHRNFI +I   +H ++   ++  P   V+LFTLPLFHVFGFFML+R  A+GETLV
Sbjct: 256  GVLLTHRNFIALIGGFYHLRMVVDDDPHP---VSLFTLPLFHVFGFFMLVRAIAVGETLV 312

Query: 864  LLEKFDFIKMLESVEKYKVTYMPVSPPIVVAMAKSEFVKKYDXXXXXXXXXXXXXXXXXX 685
             + +FDF  ML++VE+Y++TYMPVSPP+VVA+AKSE VKKYD                  
Sbjct: 313  FMHRFDFEGMLKAVERYRITYMPVSPPLVVALAKSELVKKYDMSSLRYLGSGGAPLGKEV 372

Query: 684  SERFTARFPNVEIVQXXXXXXXXXXXXGMNSPEEGKRYGSGGRLSANLQGKIVDPGTGEA 505
            +E F A+FPNVEI Q             +  P+E KR+GS GRLS N++ KIVDP TGEA
Sbjct: 373  AEDFRAQFPNVEIGQGYGLTESGGGAARVLGPDESKRHGSVGRLSENMEAKIVDPVTGEA 432

Query: 504  LPPGQQGELWLRGPIVMKGYVGDNAATAATLTSDGWLKTGDLCYFDSDGFLYIVDRLKEL 325
            L PGQ+GELWLRGP +MKGYVGD  ATA TL S+GWLKTGDLCYFDSDGFLYIVDRLKEL
Sbjct: 433  LSPGQKGELWLRGPTIMKGYVGDEKATAETLDSEGWLKTGDLCYFDSDGFLYIVDRLKEL 492

Query: 324  IKYKAYQVAPAELEHIIHSIPGVADVAVIPYPDEDAGQIPMAYVVRSPGSNISEAQIKDF 145
            IKYKAYQV PAELEHI+H+ P +AD AV+PYPDE+AGQIP+A+VVR  GSNI+  Q+ +F
Sbjct: 493  IKYKAYQVPPAELEHILHTNPEIADAAVVPYPDEEAGQIPIAFVVRKSGSNITADQVMEF 552

Query: 144  VTRQVSPYKKVRRVAFINAIPKSPAGKILRRELVNHALSAGSARL 10
            V +QVSPYKK+RRV+FI +IPKSPAGKILRRELV++ALS GS++L
Sbjct: 553  VAKQVSPYKKIRRVSFIKSIPKSPAGKILRRELVDYALSCGSSKL 597



 Score = 68.6 bits (166), Expect(3) = e-154
 Identities = 43/99 (43%), Positives = 55/99 (55%), Gaps = 2/99 (2%)
 Frame = -1

Query: 1549 PLSVTQYADSLLRSSTTF--SPETTAFLIDSATSRRVSYSEFXXXXXXXXXXXXXXXXXX 1376
            PLS+T Y  SLL S+ T   S   T+ +ID+AT R +SYS                    
Sbjct: 77   PLSLTDYVFSLLPSAATATASSSITSAIIDAATDRHLSYS-----LLLRQVKSLTSSLQS 131

Query: 1375 XXXXXXQNVALILSPPSLHIPVLYFSLMSLNVVVSPANP 1259
                   +VALIL+P SLH+PVLYFSL+SL V ++PANP
Sbjct: 132  LTPLSKGHVALILTPSSLHVPVLYFSLLSLGVTIAPANP 170



 Score = 37.4 bits (85), Expect(3) = e-154
 Identities = 18/35 (51%), Positives = 25/35 (71%), Gaps = 5/35 (14%)
 Frame = -2

Query: 1716 ISMASLQQS-----TGAVDPNSGYCSETKTFYSLR 1627
            ISMA+  Q+     T  +DPNSG+CS ++TF+SLR
Sbjct: 32   ISMATQTQTQSHPNTTTIDPNSGFCSHSRTFHSLR 66


>ref|XP_002279522.2| PREDICTED: 4-coumarate--CoA ligase-like 9-like [Vitis vinifera]
          Length = 851

 Score =  499 bits (1284), Expect(3) = e-154
 Identities = 250/388 (64%), Positives = 304/388 (78%)
 Frame = -2

Query: 1209 AKHLPKLPLGTIVVDSPEFQSLLTSSSETPPRQINISQSNTAAILYSSGTTGRVKGVELT 1030
            A  LP L LGT++VDS EF S++T +     +++ +SQS+ AAILYSSGTTGRVKGV LT
Sbjct: 155  AHKLPSLRLGTVLVDSLEFDSMMTENGAGYGKRVEVSQSDPAAILYSSGTTGRVKGVLLT 214

Query: 1029 HRNFIVVIANLFHNKISWPEEELPEQSVALFTLPLFHVFGFFMLIRGFAMGETLVLLEKF 850
            HRN I +IA   HN++   +EE   + V+LFTLPLFHVFGFFML+R  ++ ETLVL+E+F
Sbjct: 215  HRNLITLIAGFHHNRLE--KEEDGPRPVSLFTLPLFHVFGFFMLVRAVSLAETLVLMERF 272

Query: 849  DFIKMLESVEKYKVTYMPVSPPIVVAMAKSEFVKKYDXXXXXXXXXXXXXXXXXXSERFT 670
            DF  ML++VEKY++TYMPVSPP+VVA+AKSEFV KYD                  SERF+
Sbjct: 273  DFENMLKAVEKYRITYMPVSPPLVVALAKSEFVGKYDLSSLQLLGSGGAPLGKEVSERFS 332

Query: 669  ARFPNVEIVQXXXXXXXXXXXXGMNSPEEGKRYGSGGRLSANLQGKIVDPGTGEALPPGQ 490
            ARFPNV++VQ            GM   +E KR+GS GRL  +++ KIVDP TGEALPPGQ
Sbjct: 333  ARFPNVQMVQGYGLTESGGGAAGMADLDEAKRHGSVGRLMHDMEAKIVDPKTGEALPPGQ 392

Query: 489  QGELWLRGPIVMKGYVGDNAATAATLTSDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKA 310
            QGELWLRGP +MKGYVGD+ ATAATL  +GWLKTGDLCYFDSDGFL+IVDRLKELIKYKA
Sbjct: 393  QGELWLRGPTIMKGYVGDDEATAATLDQEGWLKTGDLCYFDSDGFLFIVDRLKELIKYKA 452

Query: 309  YQVAPAELEHIIHSIPGVADVAVIPYPDEDAGQIPMAYVVRSPGSNISEAQIKDFVTRQV 130
            YQV PAELEH++ S P +AD AVIPYPDE+AGQIPMA+VVR PGS+I+EAQ+ +F+ +QV
Sbjct: 453  YQVPPAELEHLLQSNPEIADAAVIPYPDEEAGQIPMAFVVRKPGSSITEAQVMEFIAKQV 512

Query: 129  SPYKKVRRVAFINAIPKSPAGKILRREL 46
            +PYKK+RRVAF+N+IPKSPAGKILRREL
Sbjct: 513  APYKKIRRVAFVNSIPKSPAGKILRREL 540



 Score = 69.7 bits (169), Expect(3) = e-154
 Identities = 41/98 (41%), Positives = 54/98 (55%)
 Frame = -1

Query: 1552 QPLSVTQYADSLLRSSTTFSPETTAFLIDSATSRRVSYSEFXXXXXXXXXXXXXXXXXXX 1373
            +PLS+ +Y  SLLRS+     ETT  LID+++  R+SYS+F                   
Sbjct: 37   RPLSLAEYVISLLRSAPDSPLETTNVLIDASSDYRLSYSQFLRQVQSLAASLQRITGLSK 96

Query: 1372 XXXXXQNVALILSPPSLHIPVLYFSLMSLNVVVSPANP 1259
                  + A IL PPSL IPV+YFSL+SL V +SP NP
Sbjct: 97   G-----DTAFILCPPSLKIPVIYFSLLSLGVTISPTNP 129



 Score = 27.3 bits (59), Expect(3) = e-154
 Identities = 10/18 (55%), Positives = 14/18 (77%)
 Frame = -2

Query: 1680 VDPNSGYCSETKTFYSLR 1627
            +DP SG+   TKT++SLR
Sbjct: 10   IDPKSGFSPTTKTYHSLR 27


>ref|XP_002511221.1| AMP dependent CoA ligase, putative [Ricinus communis]
            gi|223550336|gb|EEF51823.1| AMP dependent CoA ligase,
            putative [Ricinus communis]
          Length = 549

 Score =  484 bits (1246), Expect(3) = e-154
 Identities = 244/400 (61%), Positives = 300/400 (75%)
 Frame = -2

Query: 1209 AKHLPKLPLGTIVVDSPEFQSLLTSSSETPPRQINISQSNTAAILYSSGTTGRVKGVELT 1030
            A  +P  P GTI++DSPEF S L +S+      ++++QS+ AAILYSSGTTG+VKGV LT
Sbjct: 152  AHKIPSPPSGTILIDSPEFLSFLHNSNTAT--YVSVNQSDPAAILYSSGTTGKVKGVSLT 209

Query: 1029 HRNFIVVIANLFHNKISWPEEELPEQSVALFTLPLFHVFGFFMLIRGFAMGETLVLLEKF 850
            HRN I +I+  +HNK      E   + V+LFTLPLFHVFGFFML+R FAMGET+VL+E+F
Sbjct: 210  HRNIIALISGFYHNKGQTDPNEPEPEPVSLFTLPLFHVFGFFMLVRAFAMGETVVLMERF 269

Query: 849  DFIKMLESVEKYKVTYMPVSPPIVVAMAKSEFVKKYDXXXXXXXXXXXXXXXXXXSERFT 670
            DF  ML +VEKYKV +MPVSPP++VA+ KS+  KKYD                  S+RF 
Sbjct: 270  DFEGMLRAVEKYKVAFMPVSPPLIVALVKSDLTKKYDLSSLLFLGCGGAPLGKDVSDRFK 329

Query: 669  ARFPNVEIVQXXXXXXXXXXXXGMNSPEEGKRYGSGGRLSANLQGKIVDPGTGEALPPGQ 490
             +FP VEI Q             M SPEE K++GS GRL+ N++ KIVDP  GEALPPGQ
Sbjct: 330  DKFPQVEISQGYGLTETGGGAARMISPEEFKQHGSVGRLAENMEAKIVDPVNGEALPPGQ 389

Query: 489  QGELWLRGPIVMKGYVGDNAATAATLTSDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKA 310
            +GELWLRGP +MKGYV +  ATA TL S+GWLKTGD+CYFDS GFLYIVDRLKELIKYKA
Sbjct: 390  RGELWLRGPTLMKGYVKNEKATAETLDSEGWLKTGDICYFDSQGFLYIVDRLKELIKYKA 449

Query: 309  YQVAPAELEHIIHSIPGVADVAVIPYPDEDAGQIPMAYVVRSPGSNISEAQIKDFVTRQV 130
            YQV PAELE ++HS   +AD AV+PY DE+AGQIPMAY+VR PGS+I+EA++ DF+ +QV
Sbjct: 450  YQVPPAELEQLLHSHLEIADAAVVPYADEEAGQIPMAYIVRKPGSDITEAEVMDFIAKQV 509

Query: 129  SPYKKVRRVAFINAIPKSPAGKILRRELVNHALSAGSARL 10
            +PYKK+RRVAFINAIPKSPAGKILRRELV+ A+S  S++L
Sbjct: 510  APYKKIRRVAFINAIPKSPAGKILRRELVDLAISDHSSKL 549



 Score = 74.3 bits (181), Expect(3) = e-154
 Identities = 44/99 (44%), Positives = 58/99 (58%)
 Frame = -1

Query: 1555 DQPLSVTQYADSLLRSSTTFSPETTAFLIDSATSRRVSYSEFXXXXXXXXXXXXXXXXXX 1376
            DQPLS+ QYA SLL +STT +   T FLIDS T+  +++S+F                  
Sbjct: 34   DQPLSIAQYAVSLL-NSTTANLTATTFLIDSNTAESLTFSQFLNRTRSLSFSLKTHFSLS 92

Query: 1375 XXXXXXQNVALILSPPSLHIPVLYFSLMSLNVVVSPANP 1259
                   +VA IL P SLH+P+LYFSL+SL + +SPANP
Sbjct: 93   PN-----DVAFILCPASLHVPLLYFSLLSLGLTISPANP 126



 Score = 37.4 bits (85), Expect(3) = e-154
 Identities = 14/21 (66%), Positives = 18/21 (85%)
 Frame = -2

Query: 1689 TGAVDPNSGYCSETKTFYSLR 1627
            T  +DPN+G+C ETKTF+SLR
Sbjct: 5    TSNIDPNNGFCKETKTFHSLR 25


>ref|XP_003523137.1| PREDICTED: 4-coumarate--CoA ligase-like 9-like [Glycine max]
          Length = 580

 Score =  489 bits (1260), Expect(3) = e-153
 Identities = 250/406 (61%), Positives = 306/406 (75%), Gaps = 6/406 (1%)
 Frame = -2

Query: 1209 AKHLPKLPLGTIVVDSPEFQSLLTSSSETPPRQIN------ISQSNTAAILYSSGTTGRV 1048
            AK++P L  GTI++DSP F S+L    ET  R         +SQS++AAIL+SSGTTGRV
Sbjct: 179  AKNIPSLKFGTILLDSPFFLSML-DDDETVNRDSRAHRVEEVSQSDSAAILFSSGTTGRV 237

Query: 1047 KGVELTHRNFIVVIANLFHNKISWPEEELPEQSVALFTLPLFHVFGFFMLIRGFAMGETL 868
            KGV LTHRNFI +I   +H +     +  P   V+LFTLPLFHVFGFFML+R  A+GETL
Sbjct: 238  KGVLLTHRNFITLIGGFYHLRNVADGDPHP---VSLFTLPLFHVFGFFMLVRAIAVGETL 294

Query: 867  VLLEKFDFIKMLESVEKYKVTYMPVSPPIVVAMAKSEFVKKYDXXXXXXXXXXXXXXXXX 688
            V +++FDF  ML++VE+Y +TYMPVSPP+VVA+AKSE VKKYD                 
Sbjct: 295  VFMQRFDFEGMLKAVERYGITYMPVSPPLVVALAKSELVKKYDLSSLRYLGCGGAPLGKE 354

Query: 687  XSERFTARFPNVEIVQXXXXXXXXXXXXGMNSPEEGKRYGSGGRLSANLQGKIVDPGTGE 508
             ++ F  +FPNVEI Q             +  P+E KR+GS GRL+ N++ KIVDP TGE
Sbjct: 355  VADDFRGKFPNVEIGQGYGLTESGGGAARVLGPDESKRHGSVGRLAENMEAKIVDPVTGE 414

Query: 507  ALPPGQQGELWLRGPIVMKGYVGDNAATAATLTSDGWLKTGDLCYFDSDGFLYIVDRLKE 328
            ALPPGQ+GELWLRGP +MKGYVGD  ATA TL S+GWLKTGDLCYFDSDGFLYIVDRLKE
Sbjct: 415  ALPPGQKGELWLRGPTIMKGYVGDEKATAETLDSEGWLKTGDLCYFDSDGFLYIVDRLKE 474

Query: 327  LIKYKAYQVAPAELEHIIHSIPGVADVAVIPYPDEDAGQIPMAYVVRSPGSNISEAQIKD 148
            LIKYKAYQV PAELEHI+H+ P +AD AV+PYPDE+AGQIPMA+VVR PGSN++  Q+ +
Sbjct: 475  LIKYKAYQVPPAELEHILHTNPEIADAAVVPYPDEEAGQIPMAFVVRKPGSNVTADQVME 534

Query: 147  FVTRQVSPYKKVRRVAFINAIPKSPAGKILRRELVNHALSAGSARL 10
            FV +QVSPYKK+RRV+FI +IPKSPAGKILRRELV++ALS+GS++L
Sbjct: 535  FVAKQVSPYKKIRRVSFIKSIPKSPAGKILRRELVDYALSSGSSKL 580



 Score = 70.9 bits (172), Expect(3) = e-153
 Identities = 44/97 (45%), Positives = 56/97 (57%)
 Frame = -1

Query: 1549 PLSVTQYADSLLRSSTTFSPETTAFLIDSATSRRVSYSEFXXXXXXXXXXXXXXXXXXXX 1370
            PLS+T YA SLL ++ T    TT+ LID+AT R +SYS                      
Sbjct: 66   PLSLTDYAFSLLPAAAT----TTSALIDAATDRHLSYS-----LLLRQVQSLASSLQSLT 116

Query: 1369 XXXXQNVALILSPPSLHIPVLYFSLMSLNVVVSPANP 1259
                 +VALIL+P SLH+PVLYFSL+SL V ++PANP
Sbjct: 117  PLSKGHVALILTPSSLHVPVLYFSLLSLGVTIAPANP 153



 Score = 33.1 bits (74), Expect(3) = e-153
 Identities = 11/18 (61%), Positives = 17/18 (94%)
 Frame = -2

Query: 1680 VDPNSGYCSETKTFYSLR 1627
            +DPNSG+CS ++TF++LR
Sbjct: 38   IDPNSGFCSHSRTFHTLR 55