BLASTX nr result
ID: Bupleurum21_contig00011125
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00011125
(1670 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|ADC80920.1| delta-9 fatty acid desaturase [Vernicia fordii] 394 e-111
ref|XP_002517641.1| delta 9 desaturase, putative [Ricinus commun... 392 e-109
ref|XP_004152346.1| PREDICTED: palmitoyl-monogalactosyldiacylgly... 383 e-106
ref|NP_566529.1| palmitoyl-monogalactosyldiacylglycerol delta-7 ... 374 e-105
gb|AAK92773.1| putative delta 9 desaturase [Arabidopsis thaliana] 372 e-104
>gb|ADC80920.1| delta-9 fatty acid desaturase [Vernicia fordii]
Length = 312
Score = 394 bits (1012), Expect(2) = e-111
Identities = 177/241 (73%), Positives = 193/241 (80%)
Frame = +2
Query: 428 NFGKILLSDVVVKRRRNVYWGRKWSSMDVITITVVGSMHVLSLXXXXXXXXXXXXXXXGL 607
NF +ILLSDV VKR R V+ GRKW+++D+ T VV +MH+LSL GL
Sbjct: 17 NFRRILLSDVAVKRERKVFLGRKWNTLDISTAGVVLAMHLLSLFAPFYFNWSAFWVAFGL 76
Query: 608 YLVTGLLGITLSFHRNLSHRSFKLPKWLEYLFAYCGAQALQGHPIDWVSTHRYHHQFCDS 787
Y+VTGLLGITLSFHRNLSHRSFKLPKWLEY FAYCG QALQG+PIDWVSTHRYHHQFCDS
Sbjct: 77 YVVTGLLGITLSFHRNLSHRSFKLPKWLEYSFAYCGVQALQGNPIDWVSTHRYHHQFCDS 136
Query: 788 ERDPHSPFEGFWYSHISWFFDNKTIVERCGGPNNASDLEKQPFYKFLEKTYAFHPXXXXX 967
ERDPHSP EGFWYSH+SW FD I+ERCGGPNN DLEKQPFYKF++ TY FHP
Sbjct: 137 ERDPHSPIEGFWYSHMSWLFDTSAIIERCGGPNNVGDLEKQPFYKFIQNTYIFHPIALGV 196
Query: 968 XXXXXXXFPFLVWGMGVRTVWVYHITWLVNSACHVWGKQAWNTGDLSRNNWWVAVLAFGE 1147
FPFLVWGMGVR +WV+HITWLVNSACHVWGKQAWNTGDLSRNNWWVAVLAFGE
Sbjct: 197 LLYAIGGFPFLVWGMGVRIIWVFHITWLVNSACHVWGKQAWNTGDLSRNNWWVAVLAFGE 256
Query: 1148 G 1150
G
Sbjct: 257 G 257
Score = 35.4 bits (80), Expect(2) = e-111
Identities = 15/20 (75%), Positives = 19/20 (95%)
Frame = +3
Query: 1269 IGLATDVKLPTEVQKQRVGF 1328
IGLATDVK+P+E+QKQR+ F
Sbjct: 291 IGLATDVKVPSEMQKQRMAF 310
>ref|XP_002517641.1| delta 9 desaturase, putative [Ricinus communis]
gi|223543273|gb|EEF44805.1| delta 9 desaturase, putative
[Ricinus communis]
Length = 386
Score = 392 bits (1006), Expect(2) = e-109
Identities = 190/333 (57%), Positives = 224/333 (67%), Gaps = 10/333 (3%)
Frame = +2
Query: 182 VMVAPLPGTKPIQFSALRHPKLIKFVPSQSLTIKNSPHCHNVAKISILDNCNWRN--IIV 355
++ +P+ +KP F +R K +K S + N N + + +C N +
Sbjct: 3 LVTSPVAKSKPYPFLPIRFSKALKL----SSLVLNPSDQKNPKSVHLKTHCKRENSSLFA 58
Query: 356 CSKRVSPIVKA-------AXXXXXXXXXXXGNFGK-ILLSDVVVKRRRNVYWGRKWSSMD 511
CSK+ + A G G+ ILLSDVVVKR RNV+ GRKW+ +D
Sbjct: 59 CSKKNTRNAAAQAIRGDDGSLFESEAEVKNGMIGRRILLSDVVVKRERNVFLGRKWNLLD 118
Query: 512 VITITVVGSMHVLSLXXXXXXXXXXXXXXXGLYLVTGLLGITLSFHRNLSHRSFKLPKWL 691
+ T VV +MH+LSL GLY++TGLLGITLSFHRNLSHRSFKLPKWL
Sbjct: 119 IATAGVVLAMHLLSLFAPFYFNWSAFWVAFGLYVITGLLGITLSFHRNLSHRSFKLPKWL 178
Query: 692 EYLFAYCGAQALQGHPIDWVSTHRYHHQFCDSERDPHSPFEGFWYSHISWFFDNKTIVER 871
EYLFAYCG QALQG+PIDWVSTHRYHHQFCDSERDPHSP EGFWYSH+SW FD +I++R
Sbjct: 179 EYLFAYCGVQALQGNPIDWVSTHRYHHQFCDSERDPHSPIEGFWYSHMSWLFDTNSIIKR 238
Query: 872 CGGPNNASDLEKQPFYKFLEKTYAFHPXXXXXXXXXXXXFPFLVWGMGVRTVWVYHITWL 1051
CG PNN DLEKQPFYKF+++TY HP FPFLVWGMGVR +WV+HITWL
Sbjct: 239 CGEPNNVGDLEKQPFYKFIQQTYILHPIALATLLYAVGGFPFLVWGMGVRIIWVFHITWL 298
Query: 1052 VNSACHVWGKQAWNTGDLSRNNWWVAVLAFGEG 1150
VNSACHVWGKQ WNTGDLSRNNWWVA+LAFGEG
Sbjct: 299 VNSACHVWGKQEWNTGDLSRNNWWVALLAFGEG 331
Score = 32.3 bits (72), Expect(2) = e-109
Identities = 14/20 (70%), Positives = 16/20 (80%)
Frame = +3
Query: 1269 IGLATDVKLPTEVQKQRVGF 1328
IGLATDVK+P E QKQ+ F
Sbjct: 365 IGLATDVKVPNETQKQKKAF 384
>ref|XP_004152346.1| PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase,
chloroplastic-like [Cucumis sativus]
gi|449515927|ref|XP_004164999.1| PREDICTED:
palmitoyl-monogalactosyldiacylglycerol delta-7
desaturase, chloroplastic-like [Cucumis sativus]
Length = 380
Score = 383 bits (984), Expect(2) = e-106
Identities = 170/238 (71%), Positives = 189/238 (79%)
Frame = +2
Query: 437 KILLSDVVVKRRRNVYWGRKWSSMDVITITVVGSMHVLSLXXXXXXXXXXXXXXXGLYLV 616
+ILLSDVVVKRRR V+WGRKW+S+D+ T VV + H+L+L LY++
Sbjct: 86 RILLSDVVVKRRREVFWGRKWNSLDIGTAGVVVATHLLTLFAPFQFNWAAFWVAISLYII 145
Query: 617 TGLLGITLSFHRNLSHRSFKLPKWLEYLFAYCGAQALQGHPIDWVSTHRYHHQFCDSERD 796
TGL GITLSFHRNLSHRSFKLPKWLEYLFAYCG QALQG+PIDWVSTHRYHHQFCDSERD
Sbjct: 146 TGLFGITLSFHRNLSHRSFKLPKWLEYLFAYCGVQALQGNPIDWVSTHRYHHQFCDSERD 205
Query: 797 PHSPFEGFWYSHISWFFDNKTIVERCGGPNNASDLEKQPFYKFLEKTYAFHPXXXXXXXX 976
PHSP EGFW+SH+SW FD ++VERCG PNN DLEKQPFYKF++ TY HP
Sbjct: 206 PHSPIEGFWFSHMSWLFDTNSVVERCGEPNNVGDLEKQPFYKFIQTTYLLHPIALGALLY 265
Query: 977 XXXXFPFLVWGMGVRTVWVYHITWLVNSACHVWGKQAWNTGDLSRNNWWVAVLAFGEG 1150
FPF+VWGMGVR VWVYHITWLVNSACHVWG QAW TGDLSRNNWWVA+LAFGEG
Sbjct: 266 SMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGSQAWKTGDLSRNNWWVALLAFGEG 323
Score = 31.6 bits (70), Expect(2) = e-106
Identities = 12/18 (66%), Positives = 18/18 (100%)
Frame = +3
Query: 1269 IGLATDVKLPTEVQKQRV 1322
IGLATDVK+PT++QK+++
Sbjct: 357 IGLATDVKIPTQLQKEKL 374
>ref|NP_566529.1| palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase
[Arabidopsis thaliana] gi|62510457|sp|Q949X0.2|ADS3_ARATH
RecName: Full=Palmitoyl-monogalactosyldiacylglycerol
delta-7 desaturase, chloroplastic; AltName: Full=FAD5;
AltName: Full=Monogalactosyldiacylglycerol-specific
palmitic acid desaturase; Flags: Precursor
gi|11994357|dbj|BAB02316.1| delta 9 desaturase-like
protein [Arabidopsis thaliana] gi|24030393|gb|AAN41357.1|
putative delta 9 desaturase [Arabidopsis thaliana]
gi|48927606|dbj|BAD23903.1|
monogalactosyldiacylglycerol-specific palmitic acid
desaturase [Arabidopsis thaliana]
gi|57546098|gb|AAW51920.1|
palmitoyl-monogalactosyldiacylglycerol delta7-desaturase
[Arabidopsis thaliana] gi|332642216|gb|AEE75737.1|
palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase
[Arabidopsis thaliana]
Length = 371
Score = 374 bits (960), Expect(2) = e-105
Identities = 166/243 (68%), Positives = 191/243 (78%), Gaps = 1/243 (0%)
Frame = +2
Query: 425 GNFGKILLSDVVVKRRRNV-YWGRKWSSMDVITITVVGSMHVLSLXXXXXXXXXXXXXXX 601
G++ +I+LSDV+VK++ V +W R+W +MD + VV SMH+LSL
Sbjct: 73 GDYRRIMLSDVLVKKKEKVVWWEREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAF 132
Query: 602 GLYLVTGLLGITLSFHRNLSHRSFKLPKWLEYLFAYCGAQALQGHPIDWVSTHRYHHQFC 781
GLY+VTGLLGITLSFHRNLSH++FKLPKWLEYLFAYCGAQALQG+PIDWVSTHRYHHQFC
Sbjct: 133 GLYIVTGLLGITLSFHRNLSHKAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFC 192
Query: 782 DSERDPHSPFEGFWYSHISWFFDNKTIVERCGGPNNASDLEKQPFYKFLEKTYAFHPXXX 961
DS+RDPHSP +GFW+SH++W FD TI +RCG PNN DLEKQPFY+FL TY HP
Sbjct: 193 DSDRDPHSPLDGFWFSHMNWMFDTNTITQRCGEPNNVGDLEKQPFYRFLRTTYILHPLAL 252
Query: 962 XXXXXXXXXFPFLVWGMGVRTVWVYHITWLVNSACHVWGKQAWNTGDLSRNNWWVAVLAF 1141
FPF+VWGMGVR VWVYHITWLVNSACHVWGKQAWNTGDLS+NNWWVA LAF
Sbjct: 253 AVALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLSKNNWWVAALAF 312
Query: 1142 GEG 1150
GEG
Sbjct: 313 GEG 315
Score = 36.2 bits (82), Expect(2) = e-105
Identities = 16/20 (80%), Positives = 18/20 (90%)
Frame = +3
Query: 1269 IGLATDVKLPTEVQKQRVGF 1328
IGLATDVKLP+E QKQR+ F
Sbjct: 349 IGLATDVKLPSEAQKQRMAF 368
>gb|AAK92773.1| putative delta 9 desaturase [Arabidopsis thaliana]
Length = 371
Score = 372 bits (956), Expect(2) = e-104
Identities = 165/243 (67%), Positives = 190/243 (78%), Gaps = 1/243 (0%)
Frame = +2
Query: 425 GNFGKILLSDVVVKRRRNV-YWGRKWSSMDVITITVVGSMHVLSLXXXXXXXXXXXXXXX 601
G++ +I+LSDV+VK++ V +W R+W +MD + VV SMH+LSL
Sbjct: 73 GDYRRIMLSDVLVKKKEKVVWWEREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAF 132
Query: 602 GLYLVTGLLGITLSFHRNLSHRSFKLPKWLEYLFAYCGAQALQGHPIDWVSTHRYHHQFC 781
GLY+VTGLLGITLSFHRNLSH++FKLPKWLEYLFAYCGAQALQG+PIDWVSTHRYHHQFC
Sbjct: 133 GLYIVTGLLGITLSFHRNLSHKAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFC 192
Query: 782 DSERDPHSPFEGFWYSHISWFFDNKTIVERCGGPNNASDLEKQPFYKFLEKTYAFHPXXX 961
DS+RDPHSP +GFW+ H++W FD TI +RCG PNN DLEKQPFY+FL TY HP
Sbjct: 193 DSDRDPHSPLDGFWFGHMNWMFDTNTITQRCGEPNNVGDLEKQPFYRFLRTTYILHPLAL 252
Query: 962 XXXXXXXXXFPFLVWGMGVRTVWVYHITWLVNSACHVWGKQAWNTGDLSRNNWWVAVLAF 1141
FPF+VWGMGVR VWVYHITWLVNSACHVWGKQAWNTGDLS+NNWWVA LAF
Sbjct: 253 AVALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLSKNNWWVAALAF 312
Query: 1142 GEG 1150
GEG
Sbjct: 313 GEG 315
Score = 36.2 bits (82), Expect(2) = e-104
Identities = 16/20 (80%), Positives = 18/20 (90%)
Frame = +3
Query: 1269 IGLATDVKLPTEVQKQRVGF 1328
IGLATDVKLP+E QKQR+ F
Sbjct: 349 IGLATDVKLPSEAQKQRMAF 368