BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00011121
         (1543 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003631454.1| PREDICTED: aspartic proteinase nepenthesin-2...   605   e-171
ref|XP_002528927.1| pepsin A, putative [Ricinus communis] gi|223...   593   e-167
ref|XP_002304273.1| predicted protein [Populus trichocarpa] gi|2...   583   e-164
ref|XP_002334311.1| predicted protein [Populus trichocarpa] gi|2...   578   e-162
ref|XP_002326638.1| predicted protein [Populus trichocarpa] gi|2...   577   e-162

>ref|XP_003631454.1| PREDICTED: aspartic proteinase nepenthesin-2-like [Vitis vinifera]
          Length = 485

 Score =  605 bits (1561), Expect = e-171
 Identities = 307/494 (62%), Positives = 370/494 (74%), Gaps = 5/494 (1%)
 Frame = -3

Query: 1472 MASSVLITFLTLLLAHNIYAXXXXXXXXXXXXXXXXSQFNSTHHLLKSTSATSAAXXXXX 1293
            MASS L  F+T+ L H +++                 QFNST HLLK TSA SA      
Sbjct: 1    MASSFLFLFMTIFLTHYVFSCSAIVLLPLTHSLSKS-QFNSTPHLLKFTSARSATRFHHR 59

Query: 1292 XXXXXXHVSLPLSPGTDYTLSFSLSS---QTISLYMDTGSDVVWLPCSPFSCMLCEGK-S 1125
                   +SLPLSPG+DYTLSF+L S   Q ISLYMDTGSD+VW PC+PF C+LCEGK  
Sbjct: 60   HRQ----ISLPLSPGSDYTLSFNLGSHPPQPISLYMDTGSDLVWFPCAPFECILCEGKYD 115

Query: 1124 PSTISNLKPLNISSAAAANKVTCKSRACAAVHSHLPSSDICAMAKCPLEEIEISDCAAFS 945
             +    L P NI+S+A+   V+CKS AC+A H+ L SSD+CAMA+CPLE IE SDC++FS
Sbjct: 116  TAATGGLSPPNITSSAS---VSCKSPACSAAHTSLSSSDLCAMARCPLELIETSDCSSFS 172

Query: 944  CPSFYYAYGDGSFVAKLYSDTLNIPMSNPSLNLYNFTFGCAHAALGEPIGVAGFGRGILS 765
            CP FYYAYGDGS VA+LY D+L++P S+P L L+NFTFGCAH ALGEP+GVAGFGRG+LS
Sbjct: 173  CPPFYYAYGDGSLVARLYRDSLSMPASSP-LVLHNFTFGCAHTALGEPVGVAGFGRGVLS 231

Query: 764  LPAQLATSNPHLGNQFSYCLISHSFDSVRVRRPSPLILGRYSSDNKNKRVKKSEEELVEF 585
            LPAQLA+ +PHLGNQFSYCL+SHSFD+ RVRRPSPLILGRYS D++ K  K+   +  EF
Sbjct: 232  LPAQLASFSPHLGNQFSYCLVSHSFDADRVRRPSPLILGRYSLDDEKK--KRVGHDRGEF 289

Query: 584  TYTPLLENPTHPYFYLVGLQAISVGNRRFPAPASLTAVDKKGNGGMVVDSGTTYTMLPVE 405
             YT +L+NP HPYFY VGL+ I+VGNR+ P P  L  VD++GNGGMVVDSGTT+TMLP  
Sbjct: 290  VYTAMLDNPKHPYFYCVGLEGITVGNRKIPVPEILKRVDRRGNGGMVVDSGTTFTMLPAG 349

Query: 404  MYKLVVTEFSRRVEVFYKRASHVEDRTGLGPCYYV-DSVRKVPQLIFHFVGNSSSVVMPR 228
            +Y+ +VTEF+ R+   YKRA+ +E+RTGLGPCYY  DS  KVP +  HFVGN S+V++PR
Sbjct: 350  LYESLVTEFNHRMGRVYKRATQIEERTGLGPCYYSDDSAAKVPAVALHFVGN-STVILPR 408

Query: 227  RNYFYQFLDGGDDGKSKRKVGCVMLMNGGDFPESDGPAGLLGNYQQQGFEVMYDLENKRV 48
             NY+Y+F DG D  K KRKVGC+MLMNGGD  ES GPA  LGNYQQQGFEV+YDLE  RV
Sbjct: 409  NNYYYEFFDGRDGQKKKRKVGCLMLMNGGDEAESGGPAATLGNYQQQGFEVVYDLEKHRV 468

Query: 47   GFAERKCASLWDTL 6
            GFA RKCA LWD+L
Sbjct: 469  GFARRKCALLWDSL 482


>ref|XP_002528927.1| pepsin A, putative [Ricinus communis] gi|223531629|gb|EEF33456.1|
            pepsin A, putative [Ricinus communis]
          Length = 493

 Score =  593 bits (1529), Expect = e-167
 Identities = 287/460 (62%), Positives = 355/460 (77%), Gaps = 8/460 (1%)
 Frame = -3

Query: 1361 QFNSTHHLLKSTSATSAAXXXXXXXXXXXH----VSLPLSPGTDYTLSFSLSS---QTIS 1203
            QF STHHLLKSTS+ SA+                VSLPLSPG+DYTLSF+L+S   Q +S
Sbjct: 38   QFTSTHHLLKSTSSRSASRFQHQHQKRHLRNRHQVSLPLSPGSDYTLSFTLNSNPPQHVS 97

Query: 1202 LYMDTGSDVVWLPCSPFSCMLCEGKSPSTISNLKPLNISSAAAANKVTCKSRACAAVHSH 1023
            LY+DTGSD+VW PC PF C+LCEGK+ +T ++  P  +SS A +  V CKS AC+A HS+
Sbjct: 98   LYLDTGSDLVWFPCKPFECILCEGKAENTTASTPPPRLSSTARS--VHCKSSACSAAHSN 155

Query: 1022 LPSSDICAMAKCPLEEIEISDCAAFSCPSFYYAYGDGSFVAKLYSDTLNIPMSNPSLNLY 843
            LP+SD+CA+A CPLE IE SDC +FSCPSFYYAYGDGS VA+LY D++ +P++ PSL+L+
Sbjct: 156  LPTSDLCAIADCPLESIETSDCHSFSCPSFYYAYGDGSLVARLYHDSIKLPLATPSLSLH 215

Query: 842  NFTFGCAHAALGEPIGVAGFGRGILSLPAQLATSNPHLGNQFSYCLISHSFDSVRVRRPS 663
            NFTFGCAH AL EP+GVAGFGRG+LSLPAQLA+  P LGN+FSYCL+SHSF+S R+R PS
Sbjct: 216  NFTFGCAHTALAEPVGVAGFGRGVLSLPAQLASFAPQLGNRFSYCLVSHSFNSDRLRLPS 275

Query: 662  PLILGRYSSDNKNKRVKKSEEELVEFTYTPLLENPTHPYFYLVGLQAISVGNRRFPAPAS 483
            PLILG   SD+K KRV K +   V+F YT +L+NP HPYFY VGL+ IS+G ++ PAP  
Sbjct: 276  PLILGH--SDDKEKRVNKDD---VQFVYTSMLDNPKHPYFYCVGLEGISIGKKKIPAPEF 330

Query: 482  LTAVDKKGNGGMVVDSGTTYTMLPVEMYKLVVTEFSRRVEVFYKRASHVEDRTGLGPCYY 303
            L  VD++G+GG+VVDSGTT+TMLP  +Y  VV EF  RV   Y+RA  VED+TGLGPCYY
Sbjct: 331  LKRVDREGSGGVVVDSGTTFTMLPASLYNSVVAEFDNRVGRVYERAKEVEDKTGLGPCYY 390

Query: 302  VDSVRKVPQLIFHFVGNSSSVVMPRRNYFYQFLDGGDDGKSKRKVGCVMLMNGGDFPE-S 126
             D+V  +P L+ HFVGN SSVV+P++NYFY FLDGGD  + KR+VGC+MLMNGG+  E +
Sbjct: 391  YDTVVNIPSLVLHFVGNESSVVLPKKNYFYDFLDGGDGVRRKRRVGCLMLMNGGEEAELT 450

Query: 125  DGPAGLLGNYQQQGFEVMYDLENKRVGFAERKCASLWDTL 6
             GP   LGNYQQ GFEV+YDLE +RVGFA RKCASLW++L
Sbjct: 451  GGPGATLGNYQQHGFEVVYDLEQRRVGFARRKCASLWESL 490


>ref|XP_002304273.1| predicted protein [Populus trichocarpa] gi|222841705|gb|EEE79252.1|
            predicted protein [Populus trichocarpa]
          Length = 496

 Score =  583 bits (1502), Expect = e-164
 Identities = 288/460 (62%), Positives = 348/460 (75%), Gaps = 8/460 (1%)
 Frame = -3

Query: 1361 QFNSTHHLLKSTSATSAAXXXXXXXXXXXH----VSLPLSPGTDYTLSFSLSSQTISLYM 1194
            QF STHHLLKSTS  S             H    VSLPLSPG+DYTLSF+++SQ ISLY+
Sbjct: 38   QFTSTHHLLKSTSTRSTTRFHHHHHNKNSHNHRQVSLPLSPGSDYTLSFTINSQPISLYL 97

Query: 1193 DTGSDVVWLPCSPFSCMLCEGKSPS-TISNLKPLNISSAAAANKVTCKSRACAAVHSHLP 1017
            DTGSD+VW PC PF C+LCEGK+ + ++++  P  +S  A    V+CKS AC+AVHS+LP
Sbjct: 98   DTGSDLVWFPCQPFECILCEGKAENASLASTPPPKLSKTATP--VSCKSSACSAVHSNLP 155

Query: 1016 SSDICAMAKCPLEEIEISDCAAFSCPSFYYAYGDGSFVAKLYSDTLNIPMSNPSLNLYN- 840
            SSD+CA++ CPLE IEISDC   SCP FYYAYGDGS +A+LY D++ +P+SN +  ++N 
Sbjct: 156  SSDLCAISNCPLESIEISDCRKHSCPQFYYAYGDGSLIARLYRDSIRLPLSNQTNLIFNN 215

Query: 839  FTFGCAHAALGEPIGVAGFGRGILSLPAQLATSNPHLGNQFSYCLISHSFDSVRVRRPSP 660
            FTFGCAH  L EPIGVAGFGRG+LSLPAQLAT +P LGNQFSYCL+SHSFDS RVRRPSP
Sbjct: 216  FTFGCAHTTLAEPIGVAGFGRGVLSLPAQLATLSPQLGNQFSYCLVSHSFDSDRVRRPSP 275

Query: 659  LILGRYSSDNKNKRVKKSEEELVEFTYTPLLENPTHPYFYLVGLQAISVGNRRFPAPASL 480
            LILGRY  D K +RV   ++    F YT +L+NP HPYFY VGL+ IS+G ++ PAP  L
Sbjct: 276  LILGRYDHDEKERRVNGVKKP--SFVYTSMLDNPRHPYFYCVGLEGISIGRKKIPAPDFL 333

Query: 479  TAVDKKGNGGMVVDSGTTYTMLPVEMYKLVVTEFSRRVEVFYKRASHVEDRTGLGPCYYV 300
              VD+KG+GG+VVDSGTT+TMLP  +Y  VV EF  RV    +RAS +E+ TGL PCYY 
Sbjct: 334  RKVDRKGSGGVVVDSGTTFTMLPASLYDFVVAEFENRVGRVNERASVIEENTGLSPCYYF 393

Query: 299  D-SVRKVPQLIFHFVGNSSSVVMPRRNYFYQFLDGGDDGKSKRKVGCVMLMNGGDFPE-S 126
            D +V  VP+++ HFVGN SSVV+PRRNYFY+FLDGG     KRKVGC+MLMNGGD  E S
Sbjct: 394  DNNVVNVPRVVLHFVGNGSSVVLPRRNYFYEFLDGGHGKGKKRKVGCLMLMNGGDEAELS 453

Query: 125  DGPAGLLGNYQQQGFEVMYDLENKRVGFAERKCASLWDTL 6
             GP   LGNYQQQGFEV+YDLEN+RVGFA R+CASLW+ L
Sbjct: 454  GGPGATLGNYQQQGFEVVYDLENRRVGFARRQCASLWEAL 493


>ref|XP_002334311.1| predicted protein [Populus trichocarpa] gi|222871031|gb|EEF08162.1|
            predicted protein [Populus trichocarpa]
          Length = 496

 Score =  578 bits (1490), Expect = e-162
 Identities = 288/460 (62%), Positives = 345/460 (75%), Gaps = 8/460 (1%)
 Frame = -3

Query: 1361 QFNSTHHLLKSTSATSAAXXXXXXXXXXXH----VSLPLSPGTDYTLSFSLSSQTISLYM 1194
            QF STHHL+KSTS +S             H    VSLPLSPG+DYTLSF+L SQ I LY+
Sbjct: 38   QFTSTHHLIKSTSTSSITRFRRHHHQKNTHNHRQVSLPLSPGSDYTLSFTLDSQPIFLYL 97

Query: 1193 DTGSDVVWLPCSPFSCMLCEGKSPST-ISNLKPLNISSAAAANKVTCKSRACAAVHSHLP 1017
            DTGSD+VW PC PF C+LCEGK+ +T +++  P  +S  A    V+CKS AC+A HS+LP
Sbjct: 98   DTGSDLVWFPCQPFECILCEGKAENTSLASTPPPKLSKTATP--VSCKSSACSAAHSNLP 155

Query: 1016 SSDICAMAKCPLEEIEISDCAAFSCPSFYYAYGDGSFVAKLYSDTLNIPMSNPS-LNLYN 840
            SSD+CA++ CPLE IE SDC   SCP FYYAYGDGS +A+LY D++++P+SNP+ L + N
Sbjct: 156  SSDLCAISNCPLESIETSDCQKHSCPQFYYAYGDGSLIARLYRDSISLPLSNPTNLIVNN 215

Query: 839  FTFGCAHAALGEPIGVAGFGRGILSLPAQLATSNPHLGNQFSYCLISHSFDSVRVRRPSP 660
            FTFGCAH AL EPIGVAGFGRG+LSLPAQLAT +P LGNQFSYCL+SHSFDS R+RRPSP
Sbjct: 216  FTFGCAHTALAEPIGVAGFGRGVLSLPAQLATLSPQLGNQFSYCLVSHSFDSDRLRRPSP 275

Query: 659  LILGRYSSDNKNKRVKKSEEELVEFTYTPLLENPTHPYFYLVGLQAISVGNRRFPAPASL 480
            LILGRY  D K +RV    +    F YT +L+N  HPYFY VGL+ IS+G ++ PAP  L
Sbjct: 276  LILGRYDHDEKERRVNGVNKP--RFVYTSMLDNLEHPYFYCVGLEGISIGRKKIPAPGFL 333

Query: 479  TAVDKKGNGGMVVDSGTTYTMLPVEMYKLVVTEFSRRVEVFYKRASHVEDRTGLGPCYYV 300
              VD +G+GG+VVDSGTT+TMLP  +Y  VV EF  RV    +RA  +E+ TGL PCYY 
Sbjct: 334  RKVDGEGSGGLVVDSGTTFTMLPASLYGSVVAEFENRVGRVNERARVIEEDTGLSPCYYF 393

Query: 299  D-SVRKVPQLIFHFVGNSSSVVMPRRNYFYQFLDGGDDGKSKRKVGCVMLMNGGDFPE-S 126
            D +V  VP ++ HFVGN SSVV+PRRNYFY+FLDGGD    KRKVGC+MLMNGGD  E S
Sbjct: 394  DNNVVNVPSVVLHFVGNGSSVVLPRRNYFYEFLDGGDGKGKKRKVGCLMLMNGGDEAELS 453

Query: 125  DGPAGLLGNYQQQGFEVMYDLENKRVGFAERKCASLWDTL 6
             GP   LGNYQQQGFEV+YDLENKRVGFA R+CASLW+TL
Sbjct: 454  GGPGATLGNYQQQGFEVVYDLENKRVGFARRQCASLWETL 493


>ref|XP_002326638.1| predicted protein [Populus trichocarpa] gi|222833960|gb|EEE72437.1|
            predicted protein [Populus trichocarpa]
          Length = 496

 Score =  577 bits (1486), Expect = e-162
 Identities = 287/460 (62%), Positives = 345/460 (75%), Gaps = 8/460 (1%)
 Frame = -3

Query: 1361 QFNSTHHLLKSTSATSAAXXXXXXXXXXXH----VSLPLSPGTDYTLSFSLSSQTISLYM 1194
            QF STHHL+KSTS +S             H    VSLPLSPG+DYTLSF+L SQ I LY+
Sbjct: 38   QFTSTHHLIKSTSTSSITRFRRHHHQKNTHNHRQVSLPLSPGSDYTLSFTLDSQPIFLYL 97

Query: 1193 DTGSDVVWLPCSPFSCMLCEGKSPST-ISNLKPLNISSAAAANKVTCKSRACAAVHSHLP 1017
            DTGSD+VW PC PF C+LCEGK+ +T +++  P  +S  A    V+CKS AC+A HS+LP
Sbjct: 98   DTGSDLVWFPCQPFECILCEGKAENTSLASTPPPKLSKTATP--VSCKSSACSAAHSNLP 155

Query: 1016 SSDICAMAKCPLEEIEISDCAAFSCPSFYYAYGDGSFVAKLYSDTLNIPMSNPS-LNLYN 840
            SSD+CA++ CPLE IE SDC   SCP FYYAYGDGS +A+LY D++++P+SNP+ L + N
Sbjct: 156  SSDLCAISNCPLESIETSDCQKHSCPQFYYAYGDGSLIARLYRDSISLPLSNPTNLIVNN 215

Query: 839  FTFGCAHAALGEPIGVAGFGRGILSLPAQLATSNPHLGNQFSYCLISHSFDSVRVRRPSP 660
            FTFGCAH AL EPIGVAGFGRG+LSLPAQLAT +P LGNQFSYCL+SHSFDS R+RRPSP
Sbjct: 216  FTFGCAHTALAEPIGVAGFGRGVLSLPAQLATLSPQLGNQFSYCLVSHSFDSDRLRRPSP 275

Query: 659  LILGRYSSDNKNKRVKKSEEELVEFTYTPLLENPTHPYFYLVGLQAISVGNRRFPAPASL 480
            LILGRY  D K +RV    +    F YT +L+N  HPYFY VGL+ IS+G ++ PAP  L
Sbjct: 276  LILGRYDHDEKERRVNGVNKP--RFVYTSMLDNLEHPYFYCVGLEGISIGRKKIPAPGFL 333

Query: 479  TAVDKKGNGGMVVDSGTTYTMLPVEMYKLVVTEFSRRVEVFYKRASHVEDRTGLGPCYYV 300
              VD +G+GG+VVDSGTT+TMLP  +Y  VV EF  RV    +RA  +E+ TGL PCYY 
Sbjct: 334  RKVDGEGSGGLVVDSGTTFTMLPASLYGSVVAEFENRVGRVNERARVIEEDTGLSPCYYF 393

Query: 299  D-SVRKVPQLIFHFVGNSSSVVMPRRNYFYQFLDGGDDGKSKRKVGCVMLMNGGDFPE-S 126
            D +V  VP ++ HFVGN SSVV+PRRNYFY+FLDGGD    KRKVGC+MLMNGG+  E S
Sbjct: 394  DNNVVNVPSVVLHFVGNGSSVVLPRRNYFYEFLDGGDGKGKKRKVGCLMLMNGGEEAELS 453

Query: 125  DGPAGLLGNYQQQGFEVMYDLENKRVGFAERKCASLWDTL 6
             GP   LGNYQQQGFEV+YDLENKRVGFA R+CASLW+TL
Sbjct: 454  GGPGATLGNYQQQGFEVVYDLENKRVGFARRQCASLWETL 493