BLASTX nr result
ID: Bupleurum21_contig00011113
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00011113
(1508 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_003635424.1| PREDICTED: LOW QUALITY PROTEIN: putative rib... 278 3e-76
emb|CCA66036.1| hypothetical protein [Beta vulgaris subsp. vulga... 241 6e-64
emb|CCA66050.1| hypothetical protein [Beta vulgaris subsp. vulga... 232 5e-63
gb|AAD21778.1| putative non-LTR retroelement reverse transcripta... 224 8e-58
ref|XP_003612608.1| Replication protein A 70 kDa DNA-binding sub... 214 9e-57
>ref|XP_003635424.1| PREDICTED: LOW QUALITY PROTEIN: putative ribonuclease H protein
At1g65750-like [Vitis vinifera]
Length = 820
Score = 278 bits (712), Expect(2) = 3e-76
Identities = 163/438 (37%), Positives = 221/438 (50%), Gaps = 14/438 (3%)
Frame = +1
Query: 1 GFRKLRQFNLALLAKQGWRLLNNPDSLVGKFYKAVYYPDCSFLEAAIQDNCSFIWRSIMD 180
GFRKL FNLALLAKQGWR L NPDSLV + ++A Y+ + SFL A + N S++WRSI+
Sbjct: 322 GFRKLXDFNLALLAKQGWRFLRNPDSLVTRIFQARYFRNSSFLNAELGSNPSYMWRSILA 381
Query: 181 AKPVLLKGICRFIGSGVDTRIWEDPWLPDRENPFIESVRLEGFELEKVADLLKERRWDLD 360
A+ +L +G I SG ++W D WLPD N I + + GF+ KV +L+ E W D
Sbjct: 382 AQGLLKRGCYWSIASGTKVQVWGDSWLPDSSNRLIITPPVAGFDGIKVDELITEGLWRED 441
Query: 361 LIRGVFSPRDQQLIAQIPLSARSSMDGWYWAEDRKGIYSVKSGY----------KLIMTD 510
IR F RD LI IPL S D W+ D +G Y+ +SGY L+ +D
Sbjct: 442 FIRDKFMARDADLILSIPLPMSSREDQISWSFDARGEYTARSGYGALRCFRQSTALVASD 501
Query: 511 FMNNIPHHSSPHWPSVWNLVVPPKVKNFLWRAISHCLPTMVNLQNRHVPVSELCPLCHQE 690
+N W +W + PPK+ NF WRA +CLPT L RHV CP+C E
Sbjct: 502 VDSNFV------WAQLWKVTAPPKILNFAWRAARNCLPTRFALTIRHVDTPMCCPICRSE 555
Query: 691 EEDTFHVLCDCSQARAVWRSTPMGDXXXXXXXXXXXXXLLVNKSREVVDLA-AVICWVLW 867
E T H L +C AR VW + + + VV +CW LW
Sbjct: 556 LETTLHALVECVAARDVWDESGLAMLQGNFGSFVDWLATMFAYCDFVVFAKYLAVCWGLW 615
Query: 868 CHRNEVVWENKISPTWLLLNRAGAALDAWRQAQVKGGTDGVAVVSV---WTAPPRGLLKL 1038
RN+VVW +I + ++N L++W A T V V S W P G +K+
Sbjct: 616 WRRNDVVWNGRIWHSQQVVNGCFTMLESWFHANETLAT-AVTVPSYSSKWQKPDYGWIKI 674
Query: 1039 NIDAAVFVDEGKAGFGCLLRDATGSFVAACNGSVEFHPDATMVEALSIREALSWIKANGW 1218
N+D AVF D+G G + RD G F+ +VEAL +RE LSWI
Sbjct: 675 NVDGAVFPDKG--AIGAVFRDHQGRFMGGFAKPFPHQTLPKVVEALGVREVLSWIHERSR 732
Query: 1219 TNVFIESDSMVVVQSMAH 1272
+ + +E+D + VVQ++ H
Sbjct: 733 SRIVVETDCLRVVQAIQH 750
Score = 35.0 bits (79), Expect(2) = 3e-76
Identities = 19/59 (32%), Positives = 28/59 (47%)
Frame = +3
Query: 1302 FNAIVRDCWCLLNELSNSSIAFVRRSANQAAHVLARNVLNSFAREECFVVPPAFLCNTL 1478
F I+ DC +L L + + + RRSAN AAH LA + + PP + + L
Sbjct: 758 FGFIIVDCLDVLQHLVDVQVVYARRSANSAAHCLANGACSFTSLHIWGYTPPQCILSCL 816
>emb|CCA66036.1| hypothetical protein [Beta vulgaris subsp. vulgaris]
Length = 1369
Score = 241 bits (614), Expect(2) = 6e-64
Identities = 145/429 (33%), Positives = 218/429 (50%), Gaps = 15/429 (3%)
Frame = +1
Query: 1 GFRKLRQFNLALLAKQGWRLLNNPDSLVGKFYKAVYYPDCSFLEAAIQDNCSFIWRSIMD 180
G R FN ALLAKQ WR+L PDSL+ + K Y+P +FLEA + N SF +SI+
Sbjct: 863 GIRNFDVFNRALLAKQAWRILTKPDSLMARVIKGKYFPRSNFLEARVSPNMSFTCKSILS 922
Query: 181 AKPVLLKGICRFIGSGVDTRIWEDPWLPDRENPFIESVR--LEGFELEKVADLLKERRWD 354
A+ V+ KG+CR IG G DT IW DPW+P E I + E +KV +L+ RW+
Sbjct: 923 ARAVIQKGMCRVIGDGRDTTIWGDPWVPSLERYSIAATEGVSEDDGPQKVCELISNDRWN 982
Query: 355 LDLIRGVFSPRDQQLIAQIPLSARSSMDGWYWAEDRKGIYSVKSGYKLIMTDFMNNIPHH 534
++L+ +F P + I +IP++ + D W W + G ++V+S Y + + P
Sbjct: 983 VELLNTLFQPWESTAIQRIPVALQKKPDQWMWMMSKNGQFTVRSAYYHELLEDRKTGPST 1042
Query: 535 S-SPH---WPSVWNLVVPPKVKNFLWRAISHCLPTMVNLQNRHVPVSELCPLCHQEEEDT 702
S P+ W +W +PPKVK F W+AI + L N++ R + + CP C ++EE T
Sbjct: 1043 SRGPNLKLWQKIWKAKIPPKVKLFSWKAIHNGLAVYTNMRKRGMNIDGACPRCGEKEETT 1102
Query: 703 FHVLCDCSQARAVWRSTPM----GDXXXXXXXXXXXXXLLVNKSREVVDLAAVICWVLWC 870
H++ C ++ W +P+ G+ L +K E L +ICW +W
Sbjct: 1103 EHLIWGCDESSRAWYISPLRIHTGNIEAGSFRIWVESLLDTHKDTEWWALFWMICWNIWL 1162
Query: 871 HRNEVVWENKISPTWLLLNRAGAALDAWRQAQVKGGTDGVAVVSV----WTAPPRGLLKL 1038
RN+ V+E K ++ RA + + + T V ++ W+ PP G++KL
Sbjct: 1163 GRNKWVFEKKKLAFQEVVERAVRGVMEFEEECAH--TSPVETLNTHENGWSVPPVGMVKL 1220
Query: 1039 NIDAAVFVDEGKAGFGCLLRDATGS-FVAACNGSVEFHPDATMVEALSIREALSWIKANG 1215
N+DAAVF G G G ++RDA G +A C G D M EA S+R L G
Sbjct: 1221 NVDAAVFKHVG-IGMGGVVRDAEGDVLLATCCGGWAME-DPAMAEACSLRYGLKVAYEAG 1278
Query: 1216 WTNVFIESD 1242
+ N+ +E D
Sbjct: 1279 FRNLVVEMD 1287
Score = 31.6 bits (70), Expect(2) = 6e-64
Identities = 15/47 (31%), Positives = 26/47 (55%)
Frame = +3
Query: 1293 LSPFNAIVRDCWCLLNELSNSSIAFVRRSANQAAHVLARNVLNSFAR 1433
++PF +V D L ++ SN V+R N+ AH+LA+ N+ +
Sbjct: 1302 VTPFGRVVDDILYLASKCSNVVFEHVKRHCNKVAHLLAQMCKNAMEK 1348
>emb|CCA66050.1| hypothetical protein [Beta vulgaris subsp. vulgaris]
Length = 1357
Score = 232 bits (592), Expect(2) = 5e-63
Identities = 149/430 (34%), Positives = 215/430 (50%), Gaps = 8/430 (1%)
Frame = +1
Query: 1 GFRKLRQFNLALLAKQGWRLLNNPDSLVGKFYKAVYYPDCSFLEAAIQDNCSFIWRSIMD 180
GF+ L FN ALL KQ WRLL+N +SL+ + A YYP A + + S+ WRSI
Sbjct: 861 GFKDLAVFNDALLGKQVWRLLHNKESLLSRVMSAKYYPHGDVRYARLGYSHSYSWRSIWG 920
Query: 181 AKPVLLKGICRFIGSGVDTRIWEDPWLPDRENPFIESVRLEGFELEKVADLL--KERRWD 354
AK ++L+G+ +G G IW PW+ D E FI+S R+EG LE V DL+ + + W+
Sbjct: 921 AKSLVLEGLIWRVGDGTKIDIWSAPWVGDEEGRFIKSARVEG--LEVVGDLMDVERKEWN 978
Query: 355 LDLIRGVFSPRDQQLIAQIPLSARSSMDGWYWAEDRKGIYSVKSGYKLIMTDFMNNIPHH 534
++LI F+ RDQQ I IPLS R D WA + G YSVK+ Y L +++
Sbjct: 979 VELIERHFNERDQQCILAIPLSTRCLQDELTWAYSKDGTYSVKTAYMLGKGGNLDDFHR- 1037
Query: 535 SSPHWPSVWNLVVPPKVKNFLWRAISHCLPTMVNLQNRHVPVSELCPLCHQEEEDTFHVL 714
W +W+L V PKV++FLWRA + LP LQ RH+ CP C +E+E FH+
Sbjct: 1038 ---VWNILWSLNVSPKVRHFLWRACTSSLPVRKVLQRRHLIDEAGCPCCAREDETQFHLF 1094
Query: 715 CDCSQARAVWRSTPMGDXXXXXXXXXXXXXLL--VNKSREVVDLAAVICWVLWCHRNEVV 888
C + +W L+ +VV I W +W RN V
Sbjct: 1095 YRCPMSLKLWEELGSYILLPGIEDEAMCDTLVRWSQMDAKVVQKGCYILWNVWVERNRRV 1154
Query: 889 WENKISPTWLLLNRAGAALDAWRQAQVK--GGTDGVAVVSV--WTAPPRGLLKLNIDAAV 1056
+E+ P ++ R ++ + VK GG A +S W APP G +KLN DA++
Sbjct: 1155 FEHTSQPATVVGQRIMRQVEDFNNYAVKIYGGMRSSAALSPSRWYAPPVGAIKLNTDASL 1214
Query: 1057 FVDEGKAGFGCLLRDATGSFVAACNGSVEFHPDATMVEALSIREALSWIKANGWTNVFIE 1236
+EG G G + RD+ G A V + + E +I A +A+G+ +V E
Sbjct: 1215 -AEEGWVGLGVIARDSEGKVCFAATRRVRAYWPPEVAECKAIYMATRLAQAHGYGDVIFE 1273
Query: 1237 SDSMVVVQSM 1266
SDS+V + +
Sbjct: 1274 SDSLVATKRL 1283
Score = 37.0 bits (84), Expect(2) = 5e-63
Identities = 27/72 (37%), Positives = 36/72 (50%), Gaps = 6/72 (8%)
Frame = +3
Query: 1287 ILLSPFNAIVRDCWCLLNELSNSSIAFVRRSANQAAHVLARNVLNSFAREECF------V 1448
I S +AI+ D + N S+ S + V+R N AH LAR V F E+C+
Sbjct: 1288 IFFSDLDAILGDILSMCNAFSSVSFSHVKRDGNTVAHNLARVV--PFGVEQCWEHHCPSS 1345
Query: 1449 VPPAFLCNTLSL 1484
V P L +TLSL
Sbjct: 1346 VTPYVLMDTLSL 1357
>gb|AAD21778.1| putative non-LTR retroelement reverse transcriptase [Arabidopsis
thaliana]
Length = 1715
Score = 224 bits (571), Expect(2) = 8e-58
Identities = 139/447 (31%), Positives = 210/447 (46%), Gaps = 31/447 (6%)
Frame = +1
Query: 1 GFRKLRQFNLALLAKQGWRLLNNPDSLVGKFYKAVYYPDCSFLEAAIQDNCSFIWRSIMD 180
GF+ L QFN ALLAKQ WR+L NP SL+ + YK +YYP+ ++L A + S+ W SI +
Sbjct: 1198 GFKDLHQFNRALLAKQAWRILTNPQSLLARLYKGLYYPNTTYLRANKGGHASYGWNSIQE 1257
Query: 181 AKPVLLKGICRFIGSGVDTRIWEDPWLP-----DRENPFIESVRLEGFELEKVADLLKE- 342
K +L +G+ +G G T+IWEDPWLP P ++ E KVADL +E
Sbjct: 1258 GKLLLQQGLRVRLGDGQTTKIWEDPWLPTLPPRPARGPILD-------EDMKVADLWREN 1310
Query: 343 -RRWDLDLIRGVFSPRDQQLIAQIPLSARSSMDGWYWAEDRKGIYSVKSGY------KLI 501
R WD + GV +P DQQL + LS ++ D + WA R Y+V+SGY L
Sbjct: 1311 KREWDPVIFEGVLNPEDQQLAKSLYLSNYAARDSYKWAYTRNTQYTVRSGYWVATHVNLT 1370
Query: 502 MTDFMNNIPHHSSPHWPSVWNLVVPPKVKNFLWRAISHCLPTMVNLQNRHVPVSELCPLC 681
+ +N + P +W L + PK+K+F+WR +S L T L+NR++P C C
Sbjct: 1371 EEEIINPL-EGDVPLKQEIWRLKITPKIKHFIWRCLSGALSTTTQLRNRNIPADPTCQRC 1429
Query: 682 HQEEEDTFHVLCDCSQARAVWRSTPM-GDXXXXXXXXXXXXXLLVNKSREVVDLAAV--- 849
+E H++ CS A+ VWRS G L+ + ++ +L +
Sbjct: 1430 CNADETINHIIFTCSYAQVVWRSANFSGSNRLCFTDNLEENIRLILQGKKNQNLPILNGL 1489
Query: 850 ----ICWVLWCHRNEVVWENKISPTWLLLNRAGAALDAWRQAQV----------KGGTDG 987
I W LW RNE +++ W + +A W + V +
Sbjct: 1490 MPFWIMWRLWKSRNEYLFQQLDRFPWKVAQKAEQEATEWVETMVNDTAISHNTAQSNDRP 1549
Query: 988 VAVVSVWTAPPRGLLKLNIDAAVFVDEGKAGFGCLLRDATGSFVAACNGSVEFHPDATMV 1167
++ W++PP G LK N D+ G +LRD G + + ++ A
Sbjct: 1550 LSRSKQWSSPPEGFLKCNFDSGYVQGRDYTSTGWILRDCNGRVLHSGCAKLQQSYSALQA 1609
Query: 1168 EALSIREALSWIKANGWTNVFIESDSM 1248
EAL AL + G+ V+ E D++
Sbjct: 1610 EALGFLHALQMVWIRGYCYVWFEGDNL 1636
Score = 27.7 bits (60), Expect(2) = 8e-58
Identities = 16/44 (36%), Positives = 23/44 (52%)
Frame = +3
Query: 1335 LNELSNSSIAFVRRSANQAAHVLARNVLNSFAREECFVVPPAFL 1466
+ +L SSI +V R N AA L + + + E F VPP +L
Sbjct: 1662 MTKLPFSSIGYVNRERNLAADKLTKYANSMSSLYETFHVPPRWL 1705
>ref|XP_003612608.1| Replication protein A 70 kDa DNA-binding subunit [Medicago
truncatula] gi|355513943|gb|AES95566.1| Replication
protein A 70 kDa DNA-binding subunit [Medicago
truncatula]
Length = 1723
Score = 214 bits (545), Expect(2) = 9e-57
Identities = 131/431 (30%), Positives = 202/431 (46%), Gaps = 10/431 (2%)
Frame = +1
Query: 1 GFRKLRQFNLALLAKQGWRLLNNPDSLVGKFYKAVYYPDCSFLEAAIQDNCSFIWRSIMD 180
GF+ LR FN +L KQ W+ + P ++V + +KA Y+ C FL++ I N S++WR+I
Sbjct: 1256 GFKNLRAFNEGMLGKQAWKFMTEPHNIVTRLFKAKYFNKCDFLDSKIGHNPSYVWRNIWG 1315
Query: 181 AKPVLLKGICRFIGSGVDTRIWEDPWLPDR---ENPFIESVRLEGFELEKVADLL--KER 345
AK V+ +G IGSG D +W+ WL D + P ES+ E EL VADL + R
Sbjct: 1316 AKRVVSEGYKWSIGSGTDICVWDQRWLVDGGVLQKP--ESLPEEFKELT-VADLFISQTR 1372
Query: 346 RWDLDLIRGVFSPRDQQLIAQIPLSARSSMDGWYWAEDRKGIYSVKSGYKLIMTDFMNNI 525
W++ L+R + S D I P+ + + + ++
Sbjct: 1373 FWNVGLLRNLVSNEDTNRILNTPIFEPNHL-----------------------SQHVDLS 1409
Query: 526 PHHSSPHWPSVWNLVVPPKVKNFLWRAISHCLPTMVNLQNRHVPVSELCPLCHQEEEDTF 705
H +W +WNL VPPKVK FLWR+ + LPT V LQ+R V ++ C LC E+ED+
Sbjct: 1410 THRCDGNWVLLWNLKVPPKVKTFLWRSCRNALPTRVRLQDRGVNCTKTCALCENEDEDSM 1469
Query: 706 HVLCDCSQARAVWRSTPMGDXXXXXXXXXXXXXLLVNKSREVVD-----LAAVICWVLWC 870
H+ C+++R W+ + + + + + +D + A + W +W
Sbjct: 1470 HLFFYCTKSRQCWQQLGLWSKVQQKAQLNLSFGVTMFEILQELDSSQRVIWACVMWSIWK 1529
Query: 871 HRNEVVWENKISPTWLLLNRAGAALDAWRQAQVKGGTDGVAVVSVWTAPPRGLLKLNIDA 1050
RN+ +W N++ T + +R L W+ AQ +W P G K N+DA
Sbjct: 1530 QRNDCIWRNEVMTTAAVRDRGLNLLTGWQNAQ-----------DIWRKPDEGHFKCNVDA 1578
Query: 1051 AVFVDEGKAGFGCLLRDATGSFVAACNGSVEFHPDATMVEALSIREALSWIKANGWTNVF 1230
A F + + G G +RD +G V A D EA+ + A+ W K N+
Sbjct: 1579 AFFKESNRVGIGICIRDDSGRLVKARTSWSTLLLDVPEGEAIGLLYAIRWAKEQNLNNIT 1638
Query: 1231 IESDSMVVVQS 1263
E DS VV S
Sbjct: 1639 FELDSKRVVYS 1649
Score = 34.3 bits (77), Expect(2) = 9e-57
Identities = 16/40 (40%), Positives = 24/40 (60%), Gaps = 1/40 (2%)
Frame = +3
Query: 1293 LSPFNAIVRDCWCLLNEL-SNSSIAFVRRSANQAAHVLAR 1409
+S AI+R+C + +NS + F+RR AN+ H LAR
Sbjct: 1657 VSDLGAIIRECRTTFSSFFTNSRVEFIRRQANEVVHSLAR 1696